Plasmid最新文献_第10页

Corrigendum to “De novo synthesis of the complete genome of coxsackievirus A10 based on Golden Gate cloning combined with promoter reconstruction” [Plasmid 103 (2019) 17–24] “基于金门克隆结合启动子重建的柯萨奇病毒A10全基因组从头合成”的勘误表[质粒103 (2019)17-24]

IF 2.6 4区生物学

Plasmid Pub Date : 2020-01-01 DOI: 10.1016/j.plasmid.2019.102439

Meng Li , Jing Chen

引用次数: 0

A novel expression vector for Corynebacterium glutamicum with an auxotrophy complementation system 谷氨酸棒状杆菌具有营养缺失互补系统的新型表达载体

IF 2.6 4区生物学

Plasmid Pub Date : 2020-01-01 DOI: 10.1016/j.plasmid.2019.102476

Ying Li , Yuqing Ai , Junzheng Zhang , Jingxuan Fei , Bingnan Liu , Jing Wang , Meng Li , Qiancheng Zhao , Jinzhu Song

{"title":"A novel expression vector for Corynebacterium glutamicum with an auxotrophy complementation system","authors":"Ying Li , Yuqing Ai , Junzheng Zhang , Jingxuan Fei , Bingnan Liu , Jing Wang , Meng Li , Qiancheng Zhao , Jinzhu Song","doi":"10.1016/j.plasmid.2019.102476","DOIUrl":"10.1016/j.plasmid.2019.102476","url":null,"abstract":"<div>Corynebacterium glutamicum is an important industrial strain used for the production of amino acids and vitamins. Most tools developed for overexpression of genes in C. glutamicum are based on the inducible promoter regulated by the lacIq gene or contain an antibiotic resistance gene as a selection marker. These vectors are essential for rapid identification of recombinant strains and detailed study of gene functions, but, as a considerable disadvantage, these vectors are not suitable for large-scale industrial production due to the need for the addition of isopropyl-β-D-thiogalactopyranoside (IPTG) and antibiotics. In this study, the novel Escherichia coli-C. glutamicum shuttle expression vector pLY-4, derived from the expression vector pXMJ19, was constructed. The constitutive vector pLY-4 contains a large multiple cloning site, the strong promoter tacM and two selective markers: the original chloramphenicol resistance gene cat is used for molecular cloning operations, and the auxotrophy complementation marker alr, which can be stably replicated in the auxotrophic host strain without antibiotic selection pressure, is used for industrial fermentation. Heterologous expression of the gapC gene using the vector pLY-4 in C. glutamicum for L-methionine production indicated the potential application of pLY-4 in the development of C. glutamicum strain engineering and industrial fermentation.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54892750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Two novel transcriptional reporter systems for monitoring Helicobacter pylori stress responses 监测幽门螺杆菌应激反应的两种新型转录报告系统

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102442

A.M. Belova , D.V. Basmanov , V.V. Babenko , O.V. Podgorny , T.V. Mitko , K.A. Prusakov , D.V. Klinov , V.N. Lazarev

{"title":"Two novel transcriptional reporter systems for monitoring Helicobacter pylori stress responses","authors":"A.M. Belova , D.V. Basmanov , V.V. Babenko , O.V. Podgorny , T.V. Mitko , K.A. Prusakov , D.V. Klinov , V.N. Lazarev","doi":"10.1016/j.plasmid.2019.102442","DOIUrl":"10.1016/j.plasmid.2019.102442","url":null,"abstract":"<div>Helicobacter pylori, a human pathogen linked to many stomach diseases, is well adapted to colonize aggressive gastric environments, and its virulence factors contribute this adaptation. Here, we report the construction of two novel H. pylori vectors, pSv2 and pSv4, carrying a reporter gene fused to the promoters of virulence factor genes for monitoring the response of single H. pylori cells to various stresses. H. pylori cryptic plasmids were modified by the introduction of the Escherichia coli origin of replication, chloramphenicol resistance cassette, and promoterless gfp gene to produce E. coli/H. pylori shuttle vectors. The promoter regions of vacA and ureA genes encoding well-characterized H. pylori virulence factors were fused to the promoterless gfp gene. Recording the GFP fluorescence signal from the genetically modified H. pylori cells immobilized in specifically designed microfluidic devices revealed the response of transcriptional reporter systems to osmotic stress, acidic stress, elevated Ni2+ concentration or iron chelation. Our observations validate the utility of the pSv2 and pSv4 vectors to monitor the regulation of virulence factor genes in diverse strains and clinical isolates of H. pylori.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48044333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid pSfr64a and the symbiotic plasmid pSfr64b of Sinorhizobium fredii GR64 control each other's conjugative transfer through quorum-sensing elements fredii Sinorhizobium GR64的质粒pSfr64a和共生质粒pSfr64b通过群体感应元件相互控制对方的共轭转移。

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102443

Laura Cervantes , Fabiola Miranda-Sánchez , Gonzalo Torres Tejerizo , David Romero , Susana Brom

{"title":"Plasmid pSfr64a and the symbiotic plasmid pSfr64b of Sinorhizobium fredii GR64 control each other's conjugative transfer through quorum-sensing elements","authors":"Laura Cervantes , Fabiola Miranda-Sánchez , Gonzalo Torres Tejerizo , David Romero , Susana Brom","doi":"10.1016/j.plasmid.2019.102443","DOIUrl":"10.1016/j.plasmid.2019.102443","url":null,"abstract":"<div>Rhizobia are nitrogen-fixing symbionts of plants. Their genomes frequently contain large plasmids, some of which are able to perform conjugative transfer. Plasmid pSfr64a from Sinorhizobium fredii GR64 is a conjugative plasmid, whose transfer is regulated by quorum sensing genes encoded by itself (traR64a, traI64a), in the symbiotic plasmid pSfr64b (traR64b, traI64b), and in the chromosome (ngrI). Also, transfer of pSfr64b requires quorum sensing elements encoded in this plasmid (traR64b, traI64b), in pSfr64a (traR64a), and in the chromosome (ngrI). These results demonstrate that pSfr64a and the symbiotic plasmid depend on each other for conjugative transfer. Plasmid pSfr64a from S. fredii GR64 is unable to transfer from the genomic background of Rhizobium etli CFN42. Our results show that the relaxase of pRet42a is able to process the oriT of pSfr64a, and viceversa, underlining their functional similarity and suggesting that in addition to the external signals, the “cytoplasmic environment” may pose a barrier to plasmid dissemination, even if the plasmids are functional in other aspects.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47515571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Synthetic promoter for efficient and muscle-specific expression of exogenous genes 外源基因高效和肌肉特异性表达的合成启动子

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102441

Yili Liu , Yutong He , Yong Wang , Ming Liu , Mingfeng Jiang , Rong Gao , Gang Wang

{"title":"Synthetic promoter for efficient and muscle-specific expression of exogenous genes","authors":"Yili Liu , Yutong He , Yong Wang , Ming Liu , Mingfeng Jiang , Rong Gao , Gang Wang","doi":"10.1016/j.plasmid.2019.102441","DOIUrl":"10.1016/j.plasmid.2019.102441","url":null,"abstract":"<div>Synthetic promoters (SPs) have many advantages over their natural counterparts, especially with regard to transcriptional activity and tissue specificity. Here, we report a new strategy to construct SPs for efficient and muscle-specific gene expression. First, 19 nucleic acid motifs classified to 3 kinds of transcriptional regulatory elements were rationally selected. A recombinant promoter library was constructed by randomly assembling these motifs. Second, the transcriptional activities of ~1200 SPs were screened by intramuscular expression of several reporter genes in different cell lines for activity higher than that of the cytomegalovirus (CMV) promoter, with SP-301 finally identified as the strongest. A single intramuscular injection of mice with an SP-301 plasmid expressing mouse growth hormone releasing hormone accelerated mouse growth significantly over 24 days. Third, the muscle specificity of SP-301 was confirmed in transgenic mice. Finally, in comparison with the CMV promoter, SP-301 accelerated translocation and increased the level of plasmid in the nuclei of myoblast cells to a greater extent than in non-muscle cells. Altogether, the study has provided a more rational strategy to construct efficient and tissue-specific promoters, with the promoter SP-301 exhibiting promising potential for establishing an intramuscular gene expression system for therapeutic applications.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42716271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dissemination of blaKPC-2 in an NTEKPC by an IncX5 plasmid 用IncX5质粒在NTEKPC中传播blaKPC-2

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102446

Rosineide Cardoso de Souza , Andrei Nicoli Gebieluca Dabul , Camila Maria dos Santos Boralli , Luíza Zuvanov , Ilana Lopes Baratella da Cunha Camargo

{"title":"Dissemination of blaKPC-2 in an NTEKPC by an IncX5 plasmid","authors":"Rosineide Cardoso de Souza , Andrei Nicoli Gebieluca Dabul , Camila Maria dos Santos Boralli , Luíza Zuvanov , Ilana Lopes Baratella da Cunha Camargo","doi":"10.1016/j.plasmid.2019.102446","DOIUrl":"10.1016/j.plasmid.2019.102446","url":null,"abstract":"<div>blaKPC-2 is disseminated worldwide usually in Tn4401, a Tn3-family transposon, and primarily in Klebsiella pneumoniae ST258, a well-known lineage that is distributed worldwide and responsible for several outbreaks. Although occurring rarely, blaKPC-2 has been described in non-Tn4401 elements (NTEKPCs), first in China and then in a few other countries. This study reports the dissemination of a blaKPC-2-carrying NTEKPC among ST11/CG258 K. pneumoniae strains and ST1642 K. quasipneumoniae subsp. quasipneumoniae AMKP9 in an Amazonian hospital. The dissemination was due to pAMKP10, an ~48 kbp IncX5 plasmid carrying ΔISKpn6/blaKPC-2/ISKpn27 in a Tn1722-based unit. Although similar to NTEKPC-Ia from pKP048 described in China, a different transposase is present upstream of ISKpn27. Additionally, mutations were identified downstream of ISKpn27 but did not affect the blaKPC-2 promoter regions. pAMKP10 conjugated in vitro only from CG258 isolates. Since CG258 strains are generally well adapted to the hospital environment, it is significant that pAMKP10 has found its way into this clinically significant clonal group. The impact of inter- and intraspecies dissemination of NTEKPCs and IncX5 plasmids harboring carbapenem resistance genes is unknown, but monitoring these plasmids could reveal their dissemination preferences.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102446","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42861912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Seamless insert-plasmid assembly at sub-terminal homologous sequences 亚末端同源序列的无缝插入-质粒组装

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102445

Anna-Sophia Krebs , Tobias Bierig , Gabriella Collu , Roger M. Benoit

{"title":"Seamless insert-plasmid assembly at sub-terminal homologous sequences","authors":"Anna-Sophia Krebs , Tobias Bierig , Gabriella Collu , Roger M. Benoit","doi":"10.1016/j.plasmid.2019.102445","DOIUrl":"10.1016/j.plasmid.2019.102445","url":null,"abstract":"<div>The engineering of fusion proteins for structural biology and protein nanotechnology often requires seamless DNA assembly with slight variations in the domain boundaries. To improve the molecular biology workflow for such projects, we evaluated the use of sub-terminal homologous sequences (HS) for co-transformation cloning and for T5 exonuclease / Phusion DNA polymerase mediated in vitro assembly. To quantify the effects of different HS-to-ends distances on cloning efficiency, we designed a blue-white-pink screening system that allowed us to easily identify positive clones (blue colonies), negative clones resulting from circular template plasmid (pink colonies) and negative colonies originating from linearized plasmids that have recircularized without an insert (white colonies). Our experiments show that both methods are feasible with HS-to-ends distances up to at least 10 base pairs. Using a combination of co-transformation cloning at sub-terminal HS and nucleotide insertions in non-annealing primer 5′-overhangs, we integrated a fusion protein into the third intracellular loop (ICL) of a G-protein-coupled receptor (GPCR) with nine different linker boundaries, using only a single plasmid linearization reaction. This molecular cloning approach is an invaluable tool for protein engineering, protein nanotechnology and synthetic biology that extends the range of applications of DNA assembly strategies.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45463865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The biology of IncI2 plasmids shown by whole-plasmid multi-locus sequence typing 全质粒多位点序列分型显示了IncI2质粒的生物学特性

IF 2.6 4区生物学

Plasmid Pub Date : 2019-11-01 DOI: 10.1016/j.plasmid.2019.102444

Richard J. Meinersmann

{"title":"The biology of IncI2 plasmids shown by whole-plasmid multi-locus sequence typing","authors":"Richard J. Meinersmann","doi":"10.1016/j.plasmid.2019.102444","DOIUrl":"10.1016/j.plasmid.2019.102444","url":null,"abstract":"<div>IncI2 type plasmids are medium-sized (~55–80 kb) conjugative plasmids that have been found carrying important antimicrobial resistance genes but have also been frequently found as cryptic plasmids. The DNA sequences for 147 fully sequenced IncI2 plasmids were studied by a whole-plasmid multi-locus sequence typing (wpMLST) scheme. A total of 171 loci were identified of which 52 were considered core (carried by greater than 95% of the plasmids). Most of the plasmids carrying the antimicrobial gene mcr-1 were in a distinct clade while most of the antimicrobial gene free plasmids were more distantly related. However, the host strains of bacteria were disparate for both groups of plasmids, showing that conjugal transfer of IncI2 plasmid is frequent. The mcr-1 gene was likely to have been introduced into IncI2 plasmids multiple times. It was also observed that the genes for conjugation showed significant linkage disequilibrium despite substantial diversity for most of those genes. Genes associated with biofilm formation were also among the core genes. The core genes can be considered the cohesive unit that defines the IncI2 plasmid group. Given the role conjugation can play in biofilm formation, it was concluded that conjugation is an active survival strategy for IncI2 plasmids. The IncI2 plasmid will have selective advantage when the plasmid-bearing bacteria are introduced to a new animal host that carries potential conjugal mates.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42498593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Inside or outside? A new collection of Gateway vectors allowing plant protein subcellular localization or over-expression 里面还是外面？允许植物蛋白亚细胞定位或过度表达的Gateway载体的新集合。

IF 2.6 4区生物学

Plasmid Pub Date : 2019-09-01 DOI: 10.1016/j.plasmid.2019.102436

François Berthold , David Roujol , Caroline Hemmer , Elisabeth Jamet , Christophe Ritzenthaler , Laurent Hoffmann , Corinne Schmitt-Keichinger

{"title":"Inside or outside? A new collection of Gateway vectors allowing plant protein subcellular localization or over-expression","authors":"François Berthold , David Roujol , Caroline Hemmer , Elisabeth Jamet , Christophe Ritzenthaler , Laurent Hoffmann , Corinne Schmitt-Keichinger","doi":"10.1016/j.plasmid.2019.102436","DOIUrl":"10.1016/j.plasmid.2019.102436","url":null,"abstract":"<div>Transient expression of proteins based on agro-infiltration techniques has proven very efficient and straightforward to study the intrinsic properties of proteins. The level of protein expression has been enhanced by the use of vector plasmids containing virus-derived sequences and the cloning step has been facilitated by recombination technologies. The pEAQ-HT-DEST series of vectors fulfilling these improvements are vectors of choice. However, they lack the possibility to directly and easily fuse the protein of interest to a fluorescent tag or to address it to the secretion pathway. In the present work we describe the production of 15 pEAQ-HT-DEST1-based plasmids designed to use the Gateway® cloning technology and to generate high levels of fluorescent fusion protein by agro-infiltration, in planta. This collection of plasmids includes binary vectors allowing N-terminal or C-terminal fusion to the bright tags EGFP or TagRFP for cytoplasmic accumulation or secretion and represents therefore a valuable tool for subcellular localization or biochemical studies. A viral protein, the blue fluorescent protein TagBFP, the green fluorescent protein variant T-Sapphire and an Arabidopsis protein were transiently expressed in N. benthamiana to demonstrate the potential of these vectors.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43072419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Identification of a novel conjugative plasmid carrying the multiresistance gene cfr in Proteus vulgaris isolated from swine origin in China 中国猪源普通变形杆菌多抗性基因cfr的一种新型共轭质粒的鉴定。

IF 2.6 4区生物学

Plasmid Pub Date : 2019-09-01 DOI: 10.1016/j.plasmid.2019.102440

Yu Zhang , Chang-Wei Lei , Hong-Ning Wang

{"title":"Identification of a novel conjugative plasmid carrying the multiresistance gene cfr in Proteus vulgaris isolated from swine origin in China","authors":"Yu Zhang , Chang-Wei Lei , Hong-Ning Wang","doi":"10.1016/j.plasmid.2019.102440","DOIUrl":"10.1016/j.plasmid.2019.102440","url":null,"abstract":"<div>The multiresistance gene cfr has a broad host range encompassing both Gram-positive and Gram-negative bacteria, and can be located on the chromosomes or on plasmids. In this study, a novel conjugative plasmid carrying cfr, designated as pPvSC3, was characterized in a Proteus vulgaris strain isolated from swine in China. Plasmid pPvSC3 is 284,528 bp in size and harbors 10 other antimicrobial resistance genes, making it a novel plasmid that differs from all known plasmids due to its unique backbone and repA gene. BLAST analysis of the plasmid sequence shows no significant homology to any known plasmid backbone, but shows high level homology to Providencia rettgeri strain CCBH11880 Contig_9, a strain isolated from surgical wound in Brazil, 2014. There are two resistance-determining regions in pPvSC3, a cfr-containing region and a multidrug-resistant (MDR) region. The cfr-containing region is flanked by IS26, which could be looped out via IS26-mediated recombination. The MDR region harbors 10 antimicrobial resistance genes carried by various DNA segments that originated from various sources. Plasmid pPvSC3 could be successfully transferred to Escherichia coli by conjugation. In summary, we have characterized a novel conjugative plasmid pPvSC3 carrying the multiresistance gene cfr and 10 other antimicrobial resistance genes, and consider that this novel type of plasmid deserves attention.</div>","PeriodicalId":49689,"journal":{"name":"Plasmid","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.plasmid.2019.102440","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47736880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11