Scientia Agricola最新文献

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Estimating the relationship of sugarcane borer larvae and crop damage based on adult captures and climate variables 基于成虫捕获量和气候变量估算甘蔗螟虫幼虫与作物危害的关系
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-03-23 DOI: 10.1590/1678-992x-2021-0274
Henrico Luís Bizão de Assis, P. Paiva, L. L. Dinardo-Miranda, P. Yamamoto
{"title":"Estimating the relationship of sugarcane borer larvae and crop damage based on adult captures and climate variables","authors":"Henrico Luís Bizão de Assis, P. Paiva, L. L. Dinardo-Miranda, P. Yamamoto","doi":"10.1590/1678-992x-2021-0274","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0274","url":null,"abstract":": Sugarcane borer Diatraea saccharalis (F.) is the primary sugarcane pest in Brazil. To estimate the relationship between larvae in sugarcane stalks and captures of male adults of D. saccharalis , we collected samples weekly: (1) adults with one delta trap with three virgin females and three female pupae and (2) larvae in 120 stalks per plot of 12.6 hectares (355 × 355 m). The study was conducted in two sites with five plots each, in the municipalities of Nova Ponte and Tupaciguara, Minas Gerais State, Brazil, from July 2016 to May 2017. Relationships between (1) males trapped per week and the number of larvae outside of stalks (LOS) were estimated and (2) we evaluated climate variables, namely average temperature, average relative air humidity, hours with relative air humidity below 30 %, rainfall and number of rainy days, and adults and larvae of D. saccharalis . We obtained generalized linear models for LOS in autumn and for larvae inside the stalks (LIS) in spring and autumn and trapped males in both sites. A significant and direct relationship between LIS and males trapped allows predicting larvae density based on captures of males. In addition, plant damage can be estimated based on accumulated captures of males. There was a negative relationship between hours of air humidity < 30 % and larvae outside of stalks. Densities of LIS can be estimated from male captures and by the humidity variables in the trapping week. Nevertheless, the models require validation in the field.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67588920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Population dynamics, vertical distribution and damage characterization of burrower bug in peanut 花生螟虫种群动态、垂直分布及危害特征
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-03-23 DOI: 10.1590/1678-992x-2021-0161
M. D. Michelotto, D. Bolonhezi, R. S. Freitas, J. F. Santos, I. J. Godoy, C. Schwertner
{"title":"Population dynamics, vertical distribution and damage characterization of burrower bug in peanut","authors":"M. D. Michelotto, D. Bolonhezi, R. S. Freitas, J. F. Santos, I. J. Godoy, C. Schwertner","doi":"10.1590/1678-992x-2021-0161","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0161","url":null,"abstract":": Although the burrower bug ( Cyrtomenus mirabilis ) is considered a vitalsoil pest in peanut crops, Arachis hypogaea L., in South America, there is little information on its occurrence and damage characterization. This study aimed to evaluate the vertical distribution and fluctuation of the burrower bug in the soil and the damage this species causes in peanuts. Two peanut cultivars (IAC OL3 and IAC 503) were evaluated in three locations in the state of São Paulo: Pindorama, Ribeirão Preto and Votuporanga, in the 2017/18 and 2018/19 harvests. Trenches were dug 0.5 m long, 0.3 m wide and 0.3 m deep, along plant lines at four spots on each sampling date, and stratified in layers 10 cm deep. Plant samples (0.5 m) were collected and evaluated for number of pods and percentage of kernels with symptoms of insect damage. More than 85 % of the C. mirabilis population was found in depths of up to 10 cm, especially after plant fructification, and an increase in nymphs from 100 days after sowing (DAS) was seen when an increased number of maturing pods was observed. The occurrence of nymphs and adults of C. mirabilis and their damage to peanut kernels was similar in both cultivars (IAC OL3 and IAC 503), when these were harvested according to their developmental cycles.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"40 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67588364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage 饲喂高水平黄颡鱼青贮羔羊的营养、生理、血液学和生化反应
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-02-18 DOI: 10.1590/1678-992x-2021-0037
Jaíne Santos Amorim, G. C. Gois, A. F. E. Silva, Milenna Alves dos Santos, Priscila Izidro de Figuêiredo, Juliana Oliveira de Miranda, R. Rodrigues, G. G. Araújo, T. V. Voltolini
{"title":"Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage","authors":"Jaíne Santos Amorim, G. C. Gois, A. F. E. Silva, Milenna Alves dos Santos, Priscila Izidro de Figuêiredo, Juliana Oliveira de Miranda, R. Rodrigues, G. G. Araújo, T. V. Voltolini","doi":"10.1590/1678-992x-2021-0037","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0037","url":null,"abstract":": Pornunça ( Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay ( Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter – DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg −1 DM and 76.78 mg kg −1 DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67587642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Monitoring performance indicators of mechanized agricultural operations through a systemic method 通过系统的方法监测农业机械化经营绩效指标
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-02-18 DOI: 10.1590/1678-992x-2021-0143
P. Peloia, M. Milan, T. L. Romanelli, L. M. Gimenez
{"title":"Monitoring performance indicators of mechanized agricultural operations through a systemic method","authors":"P. Peloia, M. Milan, T. L. Romanelli, L. M. Gimenez","doi":"10.1590/1678-992x-2021-0143","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0143","url":null,"abstract":": Key performance indicators (KPI) are essential to decision-making in an organization, but the approach to analysis and composition used in the formulation of the KPIs can lead to errors. Analysis based only on averages does not allow for discriminating between variations that are natural to the process or special cases which require investigation. The use of control charts can identify this differentiation. However, when several charts are presented encompassing different measurement units and scales, systemic interpretation can be impaired. To assist in this interpretation, this research study aimed at proposing a method to facilitate the analysis of control charts when multiple indicators are employed in the monitoring of agricultural operations. Based on the data obtained over 26 weeks from a mechanized sugarcane ( Saccharum officinarum L.) harvesting front, six indicators were defined and analyzed through individual control charts and, systemically, through a standardized group control chart. Results show that the points identified as being outside the control zone (special causes of variation) according to the standardized group control chart were the same as those identified by the six individual charts, which demonstrates the potential of this method to summarize the information with no loss of quality of analysis.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67588614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In memoriam of Paulo Cesar Sentelhas 纪念保罗·塞萨尔·森特尔哈斯
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-02-18 DOI: 10.1590/1678-992x-v80obituary
T. L. Romanelli, J. Monteiro
{"title":"In memoriam of Paulo Cesar Sentelhas","authors":"T. L. Romanelli, J. Monteiro","doi":"10.1590/1678-992x-v80obituary","DOIUrl":"https://doi.org/10.1590/1678-992x-v80obituary","url":null,"abstract":"","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67591357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soybean extracts can improve plant development 大豆提取物具有促进植物发育的作用
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-02-18 DOI: 10.1590/1678-992x-2021-0102
André May, E. H. Silva, Nilson Aparecido Vieira Junior, E. S. D. Vilela, M. S. Santos, L. F. Coelho, Alexandre Pedrinho, B. D. Batista, R. D. S. Viana
{"title":"Soybean extracts can improve plant development","authors":"André May, E. H. Silva, Nilson Aparecido Vieira Junior, E. S. D. Vilela, M. S. Santos, L. F. Coelho, Alexandre Pedrinho, B. D. Batista, R. D. S. Viana","doi":"10.1590/1678-992x-2021-0102","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0102","url":null,"abstract":": Microbial biodiversity of an environment can contribute to plant growth and increase crop yield. Plant extracts from soybean ( Glycine max (L.) Merrill) were investigated on soybean plants grown after inoculation with these extracts. Soil samples were collected from two important Brazilian soybean-growing regions to produce the extracts used in the experiments. The extracts were produced with material collected from aboveground biomass and rhizosphere of soybean plants cultivated in a controlled greenhouse (phase 1). The extracts produced in phase 1 were applied in a sequential experiment (phase 2). Phase 2 was conducted to examine the plant microbiome after the microbial alteration process in the greenhouse through seed inoculation with the extracts produced previously. Samples of aboveground biomass were collected to determine root dry matter and crop yield. Bacterial 16S rRNA sequences were processed to determine the final microbial content of soybean. The inoculated treatments had lower species diversity; however, the phyla Firmicutes and Bacteroidetes were more abundant in the treatments than in the non-inoculated treatment. The soybean plant stem in the inoculated treatment also had a positive response to enrichment of the bacterial classes Betaproteobacteria, Bacilli and Flavobacteria. Inoculation affected the microbial composition of soybean plants. The alteration of microbiome changes revealed differences for crop yield between the inoculated and non-inoculated treatments, with up to 93.5 % higher crop yields per plant according to the extract applied.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67588050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fast-growing forest management to regulate the balance between wood production and water supply 速生林管理调节木材生产和水供应之间的平衡
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-02-18 DOI: 10.1590/1678-992x-2021-0148
C. Cassiano, R. Moreira, S. Ferraz
{"title":"Fast-growing forest management to regulate the balance between wood production and water supply","authors":"C. Cassiano, R. Moreira, S. Ferraz","doi":"10.1590/1678-992x-2021-0148","DOIUrl":"https://doi.org/10.1590/1678-992x-2021-0148","url":null,"abstract":": Increasingly, fast-growing forest plantations are able to support the wood supply but may simultaneously reduce water availability. The trade-off between wood production and water supply is more evident in areas with low water availability, high seasonal variation, or high water demand from local communities. The management regime adopted in forest plantations can either increase or reduce this trade-off. Thus, we assess herein the water and wood supply under different fast-growing forest plantation management regimes to understand how forest management practices can balance the provision of these services. The study was conducted at two catchments with a predominance of fast-growing forest plantations, namely, the mosaic management catchment (MMC) and the intensive management catchment (IMC). Rainfall and streamflow were monitored for three water years. Hydrological indexes were calculated to assess the hydrological regime of both catchments, and make inventories of the forest to assess forest growth rates. MMC had streamflow coefficients, baseflow index and baseflow stability higher than those of IMC. Mean annual wood increment was 32.73 m 3 ha –1 yr –1 in MMC, with a mean age of 15 years, and 44.40 m 3 ha –1 yr –1 in IMC at coppice in the second year. MMC hydrological indexes remained stable over the period studied, while in IMC the hydrological indexes were affected by climatic variations, mainly in drier years. MMC showed potential for supplying both water and wood. However, in IMC there was a trade-off between wood supply at the expense of the water supply. Thus, the intensity of fast-growing management can be adjusted to achieve a balance between water and wood supply on a catchment scale.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67588695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
First record of Plectris aliena Chapin, 1934 (Coleoptera: Melolonthidae) as a potential sugarcane pest in Brazil 作为潜在的甘蔗害虫,1934年巴西首次记录了一种甘蔗害虫(鞘翅目:蜜蛾科)
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-01-01 DOI: 10.1590/1678-992X-2020-0128
Gilmar V Coutinho, E. S. Gomes, C. J. Ávila, I. F. D. Silva, E. N. Costa, M. Cherman
{"title":"First record of Plectris aliena Chapin, 1934 (Coleoptera: Melolonthidae) as a potential sugarcane pest in Brazil","authors":"Gilmar V Coutinho, E. S. Gomes, C. J. Ávila, I. F. D. Silva, E. N. Costa, M. Cherman","doi":"10.1590/1678-992X-2020-0128","DOIUrl":"https://doi.org/10.1590/1678-992X-2020-0128","url":null,"abstract":"ABSTRACT: Plectris alienaChapin, 1934, a white grub that promotes serious damage to a variety of crops in North America and Australia, has been reported for the first time in Brazil. Larvae and adults of P. aliena were recorded in sugarcane crops in the state of Mato Grosso do Sul, sampled by trenches in the soil with light traps, respectively, during 2012 and 2013. Root consumption was also determined for the first, second and third instar larvae. The species is univoltine; adults were found mainly in Aug and the peak of third instar larval density was in Apr in all fields sampled. Pupae and adults of P. aliena were observed in the soil profile at depths of up to 60 and 80 cm, respectively. Larvae of P. aliena of the second and third instar consumed the primary and secondary roots of sugarcane, causing serious damage. Third instar larvae consumed 916 mg of root fresh matter, representing 72 % of the sugarcane root system. These results assist in furthering the understanding of its larval dynamics in the soil and provide support for Integrated Pest Management.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"79 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67583383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genetic structure analysis of Mauritia flexuosa natural population from the Lençóis Maranhenses region using microsatellite markers 利用微卫星标记分析Lençóis Maranhenses地区弯毛毛里求斯自然居群的遗传结构
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-01-01 DOI: 10.1590/1678-992X-2020-0112
Domingos Augusto Mendonça Castro, T. S. Costa, Ariane Santos Cardoso, H. C. C. Ramos, Jorge A. López, L. E. C. Diniz
{"title":"Genetic structure analysis of Mauritia flexuosa natural population from the Lençóis Maranhenses region using microsatellite markers","authors":"Domingos Augusto Mendonça Castro, T. S. Costa, Ariane Santos Cardoso, H. C. C. Ramos, Jorge A. López, L. E. C. Diniz","doi":"10.1590/1678-992X-2020-0112","DOIUrl":"https://doi.org/10.1590/1678-992X-2020-0112","url":null,"abstract":"ABSTRACT Buriti (Mauritia flexuosa L.f), a palm tree native to South America and widely distributed in Brazil, displays significant ecological, economic and biotechnological importance. However, the disorderly extractivism and environmental degradation of its endemic areas are leading to reductions in, and/or extinction of the buriti palm tree, causing ecological imbalance with significant economic losses for rural communities and genetic diversity. Consequently, populational genetic diversity studies have become relevant as a strategy for conserving the species. Therefore, this study evaluated the genetic structure of 10 populations from the Lencois Maranhenses region, in the state of Maranhao, Brazil, using microsatellite markers. Results indicated that eight pairs displayed a high level of polymorphism in the populations evaluated (98.5 %). The genetic diversity estimations allowed for identifying 220 alleles (average of 9.5 alleles/loci), and the heterozygosity averages observed (Ho) were lower than the heterozygosity expected (He) in the population (0.16 and 0.64) and loci levels (0.15 and 0.65), respectively. The Shannon Index (I) mean value of 1.36 indicated high diversity and genotypic richness in the populations evaluated, while the population index (FST) indicated a low value (0.05) and the fixation index pertaining to individuals indicated a high value (FIS = 0.79) exhibiting a moderate population distribution structure, and pointing to greater diversity between individuals. Based on these results, populations denominated as PA and PB presented a high genetic similarity (0.219), while populations denominated as PF and PJ exhibited more distant genetic characteristics (0.519). These results can be correlated based on prioritization of conservation of this non–domesticated species, influenced by environmental characteristics, suggesting that the genetic diversity found should be conserved in a germplasm bank, and subsequently exploited in breeding programs.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"47 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67583651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants 与紫绒芽增殖相关的16SrVII-B亚群植原体
IF 2.6 3区 农林科学
Scientia Agricola Pub Date : 2022-01-01 DOI: 10.1590/1678-992x-2020-0277
J. Ferreira, Cláudia Alves de Almeida, F. F. Oliveira, Arnaldo Fariña, E. Kitajima, I. Bedendo
{"title":"Phytoplasma of 16SrVII-B subgroup associated to shoot proliferation in Physalis peruviana plants","authors":"J. Ferreira, Cláudia Alves de Almeida, F. F. Oliveira, Arnaldo Fariña, E. Kitajima, I. Bedendo","doi":"10.1590/1678-992x-2020-0277","DOIUrl":"https://doi.org/10.1590/1678-992x-2020-0277","url":null,"abstract":"Physalis is an herbaceous species native to the Andes region. Currently, it is cultivated in various Brazilian states due to the economic interest of growers for this new fruit. Physalis plants grown in the field showed symptoms of shoot proliferation, leaf malformation, and chlorosis. Since these symptoms are commonly induced by phytoplasmas, this study investigated to confirm the presence of these prokaryotes in symptomatic plants. After DNA extraction from symptomatic and asymptomatic plants, phytoplasmas were found in all affected plants through the nested PCR. Examination by transmission electron microscopy (TEM) using appropriately prepared segments of leaf veins allowed the visualization of typical pleomorphic cells of phytoplasmas in the phloem of symptomatic plants. The computer-simulated RFLP patterns and the phylogenetic analysis allowed identifying the detected phytoplasmas as a ‘Candidatus Phytoplasma fraxini’-related strain belonging to the 16SrVII-B subgroup. Moreover, physalis was identified as an additional host species for phytoplasmas in the 16SrVII group, expanding the current knowledge on the host range of phytoplasmas in this group.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"1 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67585679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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