Ticks and Tick-borne Diseases最新文献

{"title":"Characteristics of patients consulted for suspected Lyme neuroborreliosis in an endemic area","authors":"Hélène Frahier ,&nbsp;Timothée Klopfenstein ,&nbsp;Anne-Sophie Brunel ,&nbsp;Catherine Chirouze ,&nbsp;Kevin Bouiller","doi":"10.1016/j.ttbdis.2024.102353","DOIUrl":"10.1016/j.ttbdis.2024.102353","url":null,"abstract":"<div><h3>Introduction</h3><p>Some patients with unexplained neurological symptoms sought care for presumed Lyme neuroborreliosis (LNB). We aimed to compare patients’ characteristics with and without LNB.</p></div><div><h3>Material and methods</h3><p>All patients consulting for LNB suspicion and having a lumbar puncture between 2014 and 2020 in a high endemic area of Lyme borreliosis were included in the study.</p></div><div><h3>Results</h3><p>One hundred fifty-five patients were included. Forty-five patients (29 %) had LNB (mean age: 57.6 years, 28.9 % of women) including 17 with isolated intrathecal synthesis. One hundred and ten patients had no LNB (mainly neurological (29 %) and rheumatological diseases (19 %)).</p><p>Non-neurological symptoms were similar in patients with LNB and patients with no LNB (asthenia, 31 % vs. 46 %, <em>p</em> = 0.14, arthralgia 20 % vs. 31 %, <em>p</em> = 0.14) with the exception of myalgia, which was less frequent in patients with LNB (4.4 % vs. 19.1 % <em>p</em> = 0.02). In multivariable analysis, factors associated with LNB were presence of facial nerve palsy (OR = 5.7), radiculopathy (OR = 11.3), positive Lyme serology (OR = 5.4) and duration of symptoms less than 3 months (OR = 4.48). Patients with isolated intrathecal synthesis had a longer duration of symptoms (3 vs 1 months) than patients with pleocytosis. Asthenia (5.9 % vs. 32.1 %), headaches (0 % vs. 39.3 %) neuropathic pain (17.6 % vs. 50 %) and facial palsy (11.8 % vs. 39.3 %) were less frequent in patients with isolated intrathecal synthesis than patients with pleocytosis. The presence of isolated subjective neurological symptoms (paresthesia, memory disorders, insomnia, irritability, asthenia, headaches) was reported in 7/17 (41 %) of patients with isolated intrathecal synthesis, 2/28 (7.1 %) in patients with pleocytosis and 75/110 (68 %) in patients without LNB (<em>p</em> &lt; 0.001).</p></div><div><h3>Conclusion</h3><p>More than one quarter of patients consulted for suspected LNB had non-neurologic symptoms, whether or not they have a LNB. Concerning patients with isolated intrathecal synthesis, the question of presence of sequelae with a spontaneously cured disease or an active Lyme borreliosis requiring antibiotic remain.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 5","pages":"Article 102353"},"PeriodicalIF":3.2,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000463/pdfft?md5=18060f5e01efaf20c3ef36166824ee26&pid=1-s2.0-S1877959X24000463-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Colpodella spp. in ticks (Hyalomma dromedarii) infesting camels in southern Egypt","authors":"Ahmed M. Soliman ,&nbsp;Hassan Y.A.H. Mahmoud ,&nbsp;Tatsuro Hifumi ,&nbsp;Tetsuya Tanaka","doi":"10.1016/j.ttbdis.2024.102352","DOIUrl":"10.1016/j.ttbdis.2024.102352","url":null,"abstract":"<div><p>In Egypt, tick-borne diseases pose a significant threat to human and animal health, and the threat to dromedaries (<em>Camelus dromedarius</em>), the country's dominant camelid species, is of particular concern. These animals are frequently infested with ticks, and may thus develop tick-borne diseases or become reservoirs of tick-borne pathogens. However, there is a paucity of data on tick infestation in Egyptian camels, especially in the south of the country. Accordingly, we aimed to determine the prevalence of tick infestation in southern Egyptian camel populations (in Luxor and Aswan governorates), and identify the hemoprotozoan parasites carried by camel-infesting ticks. Camels were checked for ticks during veterinary examination at quarantine and household checks, and ticks were collected from infested camels for species identification using morphological examination and PCR analyses. Tick and hemoprotozoan species were identified using Basic Local Alignment Search Tool analysis with subsequent confirmation in phylogenetic analyses. All camel-infesting ticks belonged to the species <em>Hyalomma dromedarii</em>, and were clustered with ticks of this species previously found in Egypt in a phylogenetic tree based on the 16S rRNA gene. Molecular analysis targeting the 18S rRNA gene revealed the presence of hitherto undetected hemoprotozoan parasites, <em>Colpodella</em> spp<em>.</em>, in 30/297 (10.1 %) camel-infesting ticks. In phylogenetic analysis, these <em>Colpodella</em> spp<em>.</em> were highly homologous (94–98.6 %) with <em>Colpodella</em> spp<em>.</em> previously deposited in GenBank with accession numbers OQ540590Q, MH208621, and GQ411073, which relate to <em>Colpodella</em> spp. previously detected from <em>Haemaphysalis longicornis, Rhipicephalus haemaphysaloides</em>, and humans in China. PCR analyses with spherical body protein-4 (<em>SBP-4</em>) gene-specific primers revealed <em>Babesia bovis</em> in 16/297 (5 %) of camel-infesting ticks, however, <em>Babesia bigemina</em> and <em>Theileria annulata</em> were not detected. Here, we report the first detection of <em>Colpodella</em> spp<em>.</em> in <em>H. dromedarii</em> in Egypt. Further epidemiological studies are needed to assess the risk to camels and humans, and the transmission dynamics. Based on the high tick infestation rates in Egyptian camels and the identification of previously unreported protozoan hemoparasites in ticks, we consider that the dromedary should be subject to surveillance as a sentinel species for tick-borne diseases in Egypt. Our findings underline the need for surveillance and collecting data on lesser known pathogens circulating in camel-infesting ticks, as part of a public health strategy for dealing with tick-borne diseases in Egypt.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 5","pages":"Article 102352"},"PeriodicalIF":3.2,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000451/pdfft?md5=9889e938e865eb5bc272e5cb154b0f8e&pid=1-s2.0-S1877959X24000451-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaplasma phagocytophilum in urban and peri-urban passerine birds in Ile-de-France","authors":"Clotilde Rouxel ,&nbsp;Adrien Etienne ,&nbsp;Pascal Arné ,&nbsp;Cécile Le Barzic ,&nbsp;Guillaume Girault ,&nbsp;Henri-Jean Boulouis ,&nbsp;Nadia Haddad ,&nbsp;Anne-Claire Lagrée ,&nbsp;Pierre Lucien Deshuillers","doi":"10.1016/j.ttbdis.2024.102350","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102350","url":null,"abstract":"<div><p>Wild animals in general, birds in particular, play a key role in transporting ticks and propagating tick-borne pathogens. Several studies have confirmed the infection of birds with <em>Anaplasma phagocytophilum</em>, with overall prevalence varying widely from country to country and/or study to study. This zoonotic bacterium, transmitted mainly by ticks of the genus <em>Ixodes</em>, is responsible for granulocytic anaplasmosis in humans (HGA) and domestic animals (cats, dogs, horses). The disease is also called tick-borne fever (TBF) in ruminants. Extremely rare in the USA, TBF is very common in Europe, where it causes economic losses in livestock. Conversely, HGA is well established in the USA whereas only a few less severe cases have been observed in Europe. Current typing techniques support the existence of multiple variants with differences in virulence/pathogenicity and tropism for certain tick and host species. However, epidemiological cycles remain difficult to characterize in Europe. Several studies describe a cycle apparently involving only birds in Europe, but no such study has been conducted in mainland France. Our objectives were to search for <em>A. phagocytophilum</em> in passerine birds in the Ile-de-France region and to explore their diversity using <em>groEL</em> and <em>ankA</em> gene typing and multilocus sequence typing (MLST). Various tissues (spleen, liver, and skin) were collected from cadavers of 680 passerines between March and December 2021. The presence of <em>A. phagocytophilum</em> was detected by qPCR Taqman targeting the <em>msp2</em> gene. Three blackbirds (<em>Turdus merula</em>) were found positive, representing detection rates of 0.4 % in all birds tested and 3.3 % in blackbirds. The higher frequency of detection in blackbirds could be at least partially explained by their lifestyle, as they feed on the ground. Analysis of the results of <em>groEL</em> and <em>ankA</em> typing and MLST from positive blackbirds support the hypothesis that the avian <em>A. phagocytophilum</em> strains in Ile-de-France are distinct from those found in mammals, and that they form their own cluster in Europe.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102350"},"PeriodicalIF":3.2,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000438/pdfft?md5=27248b994f1cc342e5f74b372b05b428&pid=1-s2.0-S1877959X24000438-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140893898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic patterns of serum inflammatory protein markers in children with Lyme neuroborreliosis","authors":"Tobias Fredriksson ,&nbsp;Lars Brudin ,&nbsp;Anna J. Henningsson ,&nbsp;Barbro H. Skogman ,&nbsp;Ivar Tjernberg","doi":"10.1016/j.ttbdis.2024.102349","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102349","url":null,"abstract":"<div><p>Definite diagnosis of Lyme neuroborreliosis (LNB) requires investigation of serum and cerebrospinal fluid (CSF). Thus, lumbar puncture is necessary, and requires administration of sedating drugs in children. This study aimed to investigate if a pattern of different inflammatory biomarkers in serum could contribute to the selection of children for lumbar puncture in suspected LNB.</p><p>Patients were included from a cohort of children who was previously investigated for LNB including serum and CSF sampling during the years 2010–2014. The multiplex proximity extension assay (PEA) inflammation panel Target 96 (Olink Bioscience, Uppsala, Sweden) was used to examine 92 biomarkers in serum.</p><p>Based on the presence of CSF pleocytosis and <em>Borrelia</em>-specific antibodies, patients were divided into a definite LNB group (n=61) and a non-LNB control group (n=58). Following PEA and statistical analysis with multivariate logistic regression, five biomarkers remained significant (<em>p</em> &lt; 0.001), which were included in a calculation of protein index. The index biomarkers were CST5, IL-15RA, CXCL10, DNER and CX3CL1. A receiver operating characteristic curve was constructed from the index, which showed an 80 % sensitivity and 81 % specificity. Area under the curve was 0.889.</p><p>We offer evidence that, with further refinements, patterns of serum biomarkers might help identify those children more or less likely to have LNB, perhaps ultimately decreasing the need for lumbar punctures.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102349"},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000426/pdfft?md5=ba22a16f53c7d45992aaa6cf6ae7c128&pid=1-s2.0-S1877959X24000426-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140893897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The case of Mediterranean spotted fever of the traveler returned from Zambia","authors":"Takahiro Ichikawa ,&nbsp;Yongjin Qiu ,&nbsp;Shuji Ando ,&nbsp;Yuto Takeuchi ,&nbsp;Atsushi Nagasaka","doi":"10.1016/j.ttbdis.2024.102347","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102347","url":null,"abstract":"<div><p>We report the case of a traveler who returned from Zambia and was diagnosed with Mediterranean spotted fever (MSF), an infectious disease caused by <em>Rickettsia conorii conorii.</em> The patient presented to Sapporo City General Hospital with symptoms of fever, malaise, headache, and rash. The pathogen was identified by Polymerase Chain Reaction assays and subsequent analyses. The patient improved with 10-day treatment of oral doxycycline. Although some cases of MSF have been reported in sub-Saharan Africa, none have been reported in Zambia. <em>Rhipicephalus sanguineus</em> sensu lato, the vector of the <em>Rickettsia conorii conorii</em>, has been found in various areas of Zambia. Our case report highlights the potential threat of Mediterranean spotted fever in urban areas of Zambia.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102347"},"PeriodicalIF":3.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000402/pdfft?md5=3aae0d755ea8b2109503d3df61de0f43&pid=1-s2.0-S1877959X24000402-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introducing a new Editor-in-Chief","authors":"Olaf Kahl (Managing Editor of Ticks and Tick-borne Diseases),&nbsp;Ard Nijhof (Editor-in-Chief of Ticks and Tick-borne Diseases)","doi":"10.1016/j.ttbdis.2024.102348","DOIUrl":"10.1016/j.ttbdis.2024.102348","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102348"},"PeriodicalIF":3.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000414/pdfft?md5=ddf5877a63428ec80b44fbd9c9225167&pid=1-s2.0-S1877959X24000414-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mutation associated with resistance to synthetic pyrethroids is widespread in US populations of the tropical lineage of Rhipicephalus sanguineus s.l","authors":"Nathan E. Stone ,&nbsp;Rebecca Ballard ,&nbsp;Reanna M. Bourgeois ,&nbsp;Grant L. Pemberton ,&nbsp;Ryelan F. McDonough ,&nbsp;Megan C. Ruby ,&nbsp;Laura H. Backus ,&nbsp;Andrés M. López-Pérez ,&nbsp;Darrin Lemmer ,&nbsp;Zane Koch ,&nbsp;Maureen Brophy ,&nbsp;Christopher D. Paddock ,&nbsp;Gilbert J. Kersh ,&nbsp;William L. Nicholson ,&nbsp;Jason W. Sahl ,&nbsp;Joseph D. Busch ,&nbsp;Johanna S. Salzer ,&nbsp;Janet E. Foley ,&nbsp;David M. Wagner","doi":"10.1016/j.ttbdis.2024.102344","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102344","url":null,"abstract":"<div><p>The brown dog tick, <em>Rhipicephalus sanguineus</em> sensu lato (s.l.), is an important vector for <em>Rickettsia rickettsii</em>, causative agent of Rocky Mountain spotted fever. Current public health prevention and control efforts to protect people involve preventing tick infestations on domestic animals and in and around houses. Primary prevention tools rely on acaricides, often synthetic pyrethroids (SPs); resistance to this chemical class is widespread in ticks and other arthropods. <em>Rhipicephalus sanguineus</em> s.l. is a complex that likely contains multiple unique species and although the distribution of this complex is global, there are differences in morphology, ecology, and perhaps vector competence among these major lineages. Two major lineages within <em>Rh. sanguineus</em> s.l., commonly referred to as temperate and tropical, have been documented from multiple locations in North America, but are thought to occupy different ecological niches. To evaluate potential acaricide resistance and better define the distributions of the tropical and temperate lineages throughout the US and in northern Mexico, we employed a highly multiplexed amplicon sequencing approach to characterize sequence diversity at: 1) three loci within the voltage-gated sodium channel (<em>VGSC</em>) gene, which contains numerous genetic mutations associated with resistance to SPs; 2) a region of the gamma-aminobutyric acid-gated chloride channel gene (<em>GABA-Cl</em>) containing several mutations associated with dieldrin/fipronil resistance in other species; and 3) three mitochondrial genes (COI, 12S, and 16S). We utilized a geographically diverse set of <em>Rh sanguineus</em> s.l. collected from domestic pets in the US in 2013 and a smaller set of ticks collected from canines in Baja California, Mexico in 2021. We determined that a single nucleotide polymorphism (T2134C) in domain III segment 6 of the <em>VGSC</em>, which has previously been associated with SP resistance in <em>Rh. sanguineus</em> s.l., was widespread and abundant in tropical lineage ticks (&gt;50 %) but absent from the temperate lineage, suggesting that resistance to SPs may be common in the tropical lineage. We found evidence of multiple copies of <em>GABA-Cl</em> in ticks from both lineages, with some copies containing mutations associated with fipronil resistance in other species, but the effects of these patterns on fipronil resistance in <em>Rh. sanguineus</em> s.l. are currently unknown. The tropical lineage was abundant and geographically widespread, accounting for 79 % of analyzed ticks and present at 13/14 collection sites. The temperate and tropical lineages co-occurred in four US states, and as far north as New York. None of the ticks we examined were positive for <em>Rickettsia rickettsii</em> or <em>Rickettsia massiliae</em>.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102344"},"PeriodicalIF":3.2,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000372/pdfft?md5=23fca2cbfeba744047fc69acab4c1e1f&pid=1-s2.0-S1877959X24000372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with perceived Anaplasma marginale infection and clinical anaplasmosis cases on beef operations in California","authors":"SY Chen ,&nbsp;L Forero ,&nbsp;J Davy ,&nbsp;J Stackhouse ,&nbsp;D Harvey ,&nbsp;E Abdelfattah ,&nbsp;G Maier","doi":"10.1016/j.ttbdis.2024.102346","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102346","url":null,"abstract":"<div><p>Bovine anaplasmosis is a tick-borne disease caused by <em>Anaplasma marginale</em> in the United States. The objective of this study was to use a survey tool to generate information for beef operations in California on anaplasmosis prevention and control management, including to what extent management activities were informed by perceived herd-level exposure to <em>A. marginale</em> infection or occurrence of clinical anaplasmosis cases. We mailed 2,621 questionnaires with questions on <em>Anaplasma</em> status, herd demographics, anaplasmosis control and prevention measures, and environmental factors to beef ranchers in California in October 2020. Survey-weighted chi-square tests were used to compare management differences according to perceived <em>Anaplasma</em> infection status. Generalized estimating equations (GEEs) were used to analyze whether region of California, management practices, or environmental factors were associated with reported clinical cases of anaplasmosis in the previous five years. A total of 466 questionnaires describing 749 herds were obtained and used in this study. Use of management measures, including deliberate exposure of calves to ticks, vaccination for <em>Anaplasma</em>, infection control through antibiotics in feed, maintaining a completely closed herd, blood testing for <em>Anaplasma</em> on all herd additions, and taking no anaplasmosis control and prevention measures, were significantly different between herds with or without perceived <em>A. marginale</em> infection based on producers’ self-declared status. The overall perceived prevalence for <em>Anaplasma</em> infection and reported clinical cases of anaplasmosis at the herd level was 26.0 % (95 % CI: 24.3–27.7 %) and 17.1 % (95 % CI: 15.6–18.6 %) respectively, with the highest perceived infection and case numbers reported in the Central Coast region. In the GEE model, higher odds of reporting clinical cases of anaplasmosis in the previous five years were observed in cattle located in the Central Coast region, cattle within a large herd, cattle that are treated with tick/fly control, cattle in a completely closed herd, and cattle receiving <em>Anaplasma</em> vaccine. <em>Anaplasma</em> infection and bovine anaplasmosis status may be underestimated in beef herds in California based on previous study results. Changing needles between cattle after injections and conducting blood testing for <em>Anaplasma</em> on herd additions are important <em>Anaplasma</em> management measures that are infrequently implemented in beef herds in California. The results show a need for producer education to improve producers’ awareness of bovine anaplasmosis and implement proper measures for disease control and prevention.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102346"},"PeriodicalIF":3.2,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000396/pdfft?md5=714a0a474141c27962d19479b0d03f46&pid=1-s2.0-S1877959X24000396-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk of heart failure among individuals tested for Borrelia burgdorferi sensu lato antibodies, and serum Borrelia burgdorferi sensu lato seropositive individuals; a nationwide population-based, registry-based matched cohort study","authors":"Malte M. Tetens ,&nbsp;Lars Haukali Omland ,&nbsp;Ram B. Dessau ,&nbsp;Svend Ellermann-Eriksen ,&nbsp;Nanna S. Andersen ,&nbsp;Charlotte Sværke Jørgensen ,&nbsp;Christian Østergaard ,&nbsp;Jacob Bodilsen ,&nbsp;Kirstine K. Søgaard ,&nbsp;Jette Bangsborg ,&nbsp;Alex Christian Yde Nielsen ,&nbsp;Jens Kjølseth Møller ,&nbsp;Ming Chen ,&nbsp;Jesper Hastrup Svendsen ,&nbsp;Niels Obel ,&nbsp;Anne-Mette Lebech","doi":"10.1016/j.ttbdis.2024.102345","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102345","url":null,"abstract":"<div><h3>Background</h3><p>Lyme borreliosis is a tick-borne disease caused by the bacterium Borrelia burgdorferi (Bb) sensu lato complex. Previous studies have suggested an association between Lyme borreliosis and heart failure, which have been suggested to be a possible manifestation of Lyme carditis. We aimed to investigate the risk of heart failure among individuals tested for serum Bb antibodies, and serum Bb seropositive individuals.</p></div><div><h3>Methods</h3><p>We performed a matched nationwide cohort study (Denmark, 1993–2020) and included 52,200 Bb seropositive individuals, and two age- and sex-matched comparison cohorts: 1) 104,400 Bb seronegative comparison cohort members, and 2) 261,000 population controls. We investigated the risk associated with 1) being tested for serum Bb antibodies, and 2) being Bb seropositive. Outcomes were: 1) a composite of heart failure, cardiomyopathy, and/or myocarditis diagnosis, and 2) redemption of cardiovascular medicine used for treatment of heart failure. We calculated short-term odds ratios (aOR) (within 1 month) and long-term hazard rates (aHR) (after 1 month) adjusted for age, sex, diabetes, pre-existing heart failure, and kidney disease.</p></div><div><h3>Results</h3><p>Compared with the population controls, individuals tested for Bb antibodies, regardless of the test result, had increased short-term risk of heart failure, cardiomyopathy, and myocarditis (aOR 8.3, 95 %CI: 6.7–10.2), and both increased short- and long-term risk of redemption of cardiovascular medicine (aOR 4.3, 95 %CI: 3.8–4.8, aHR 1.13, 95 % CI: 1.11–1.15). The Bb seropositive individuals had no increased short- or long-term risk of any outcome compared with Bb seronegative comparison cohort members.</p></div><div><h3>Conclusions</h3><p>In conclusion, Bb antibody tests seemed to be performed in the diagnostic work-up of heart failure, but Bb seropositivity was not associated with heart failure.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102345"},"PeriodicalIF":3.2,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000384/pdfft?md5=0362cdd6b144bef6c36e06c333963d27&pid=1-s2.0-S1877959X24000384-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140606913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A next generation sequencing assay combining Ixodes species identification with pathogen detection to support tick surveillance efforts in the United States","authors":"Lynn M. Osikowicz,&nbsp;Sarah E. Maes,&nbsp;Rebecca J. Eisen,&nbsp;Andrias Hojgaard","doi":"10.1016/j.ttbdis.2024.102343","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102343","url":null,"abstract":"<div><p>The burden of tick-borne diseases continues to increase in the United States. Tick surveillance has been implemented to monitor changes in the distribution and prevalence of human disease-causing pathogens in ticks that frequently bite humans. Such efforts require accurate identification of ticks to species and highly sensitive and specific assays that can detect and differentiate pathogens from genetically similar microbes in ticks that have not been demonstrated to be pathogenic in humans. We describe a modification to a next generation sequencing pathogen detection assay that includes a target that accurately identifies <em>Ixodes</em> ticks to species. We show that the replacement of internal control primers used to ensure assay performance with primers that also act as an internal control and can additionally differentiate tick species, retains high sensitivity and specificity, improves efficiency, and reduces costs by eliminating the need to run separate assays to screen for pathogens and for tick identification.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 4","pages":"Article 102343"},"PeriodicalIF":3.2,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000360/pdfft?md5=0db3c2f76927109901c0f598d209dbc9&pid=1-s2.0-S1877959X24000360-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140549234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}