Cell Journal最新文献

{"title":"Identification and Verification of Key Genes Underlying Methamphetamine Sensitization Using Network-Based Analysis.","authors":"Ali Mohammadian, Afsaneh Mokaram Bakhtajerdi, Zahra Mortezaei","doi":"10.22074/cellj.2024.2018281.1470","DOIUrl":"https://doi.org/10.22074/cellj.2024.2018281.1470","url":null,"abstract":"<p><strong>Objective: </strong>Methamphetamine (METH) is a highly addictive psychostimulant that alters gene expression in brain reward circuits. This study aimed to identify METH-associated transcriptional changes in the nucleus accumbens (NAc) and explore potential pharmacological interventions.</p><p><strong>Materials and methods: </strong>We conducted an <i>in silico</i> analysis of publicly available microarray data (GSE46717) from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified using limma and analyzed for functional enrichment via EnrichR. Protein-protein interaction (PPI) networks were constructed using STRING to identify hub genes, validated <i>in silico</i> with jackknife resampling. Adult male Wistar rats were injected with METH (10 mg/ kg, followed by 2.5 mg/kg after one month), and expression of selected hub genes was measured in NAc tissue using quantitative polymerase chain reaction (qPCR). Connectivity mapping was applied to identify candidate drugs reversing METH-induced transcriptional changes.</p><p><strong>Results: </strong>We identified 280 DEGs (210 upregulated, 70 downregulated). Upregulated pathways included caffeine metabolism, long-term potentiation, and cocaine addiction, whereas GABAergic and glutamatergic synapse genes were downregulated. Network analysis highlighted Fos, Crh, Oprl1, and Slc17a6 as hub genes, validated both computationally and experimentally. Connectivity mapping identified D-64131 and Mebendazole as potential therapeutics.</p><p><strong>Conclusion: </strong>METH induces substantial transcriptional alterations in the NAc, affecting synaptic signaling and addiction pathways. Integrating <i>in silico</i> network analysis with experimental validation identified robust hub genes and suggested candidate compounds for therapeutic intervention.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory T Lymphocyte-Derived Extracellular Vesicles Play A Role in Chronic Kidney Diseases and Kidney Transplantation: A Review.","authors":"Reza Hajiasghar-Sharbaf, Esmaeil Atashbasteh, Hamed Fouladseresht, Nahid Eskandari","doi":"10.22074/cellj.2026.2065015.1884","DOIUrl":"https://doi.org/10.22074/cellj.2026.2065015.1884","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) significantly affects kidney function, often leading to end-stage kidney disease (ESKD). Due to its high rates of morbidity, mortality, and economic effects, CKD is becoming the ninth most important risk factor for mortality worldwide, presenting serious healthcare issues. Regulatory T lymphocytes (Tregs) play a role in regulation, particularly in transplant tolerance and autoimmune conditions. Recent research has highlighted the potential of Tregderived vehicles (EVs) to modulate responses and promote graft tolerance during kidney transplantation. These EVs can suppress effector T lymphocytes (T effect), trigger apoptosis, and modify cytokine production to maintain balance and prevent graft rejection. Moreover, Treg-EVs have emerged as potential biomarkers for diagnosing CKD, paving the way for new diagnostic and therapeutic strategies. This study explores how Treg-derived EVs influence processes underlying CKD and kidney transplantation, highlighting their significance as useful tools.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on: miR-129-5p Modulates Fndc5 Expression in Adipose Tissue of Diabetic Mice.","authors":"Baharuddin Baharuddin, Lilies Handayani, Maher Monir Akl","doi":"10.22074/cellj.2026.2086933.2014","DOIUrl":"https://doi.org/10.22074/cellj.2026.2086933.2014","url":null,"abstract":"<p><p>A study demonstrated that increased miR-129-5p expression is associated with reduced Fndc5 expression in adipose tissue of a type 2 diabetes mellitus (T2DM) mouse model, suggesting a post-transcriptional regulatory mechanism contributing to metabolic dysfunction. This Letter discusses the strengths and limitations of these findings. Although the dual-luciferase assay provided strong evidence of a direct interaction between miR-129-5p and the <i>Fndc5</i> 3'UTR, the absence of <i>in vivo</i> loss-of-function or gain-of-function experiments limits causal interpretation. In addition, downstream metabolic consequences of reduced FNDC5/irisin signaling, including insulin signaling pathways, GLUT4 regulation, and thermogenic activity, were not comprehensively evaluated. Methodological concerns, including sample size and the lack of reported blinding procedures, may also affect reproducibility. Future studies integrating miRNA manipulation strategies, circulating irisin measurement, and pathway-based metabolic analyses are needed to strengthen the biological and translational relevance of the miR-129-5p-FNDC5 axis in T2DM.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-2"},"PeriodicalIF":1.7,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein Alleviates Brain Tissue Injury in Rats with Posterior Circulation Ischemic Vertigo by Repressing The TLR4/NF-κB Pathway.","authors":"Xinru Zhang, Yanyan Ye","doi":"10.22074/cellj.2026.2045778.1715","DOIUrl":"https://doi.org/10.22074/cellj.2026.2045778.1715","url":null,"abstract":"<p><strong>Objective: </strong>Posterior circulation ischemic vertigo (PCIV) is a common clinical condition with limited effective treatments. Baicalein, a flavonoid derived from <i>Scutellaria baicalensis</i>, possesses anti-inflammatory and neuroprotective properties; however, its effects on PCIV remain unclear. This study aimed to investigate the function of baicalein on brain tissue injury in a rat model of PCIV and to explore its underlying mechanism.</p><p><strong>Materials and methods: </strong>In this experimental study, 80 rats were randomly divided into sham operation, PCIV, Baicalin, TAK242, and Baicalin+TAK242 groups. The latency to escape from electrical stimulation, blood flow in the vestibular nucleus, and hemorheological indices were measured. Pathological changes and neuronal apoptosis were assessed by HE staining and TUNEL staining, respectively. Western blotting was used to evaluate the protein expression of toll-like receptor 4 (TLR4), phosphorylated nuclear factor-kappa B p65 (p-NF-κB p65), and phosphorylated inhibitor of kappa B alpha (p-IκBα) in rat brain tissue.</p><p><strong>Results: </strong>The Baicalin, TAK242, and Baicalin+TAK242 groups showed significantly lower values for latency to escape electrical stimulation, reduction rate of vestibular nucleus blood flow, whole blood viscosity, plasma viscosity, hematocrit, erythrocyte aggregation index, erythrocyte deformability index, erythrocyte sedimentation rate, number of TUNELpositive cells, levels of LAC, LDH, IL-1β, IL-6, TNF-α, and protein expression of TLR4, p-NF-κB p65, and p-IκBα. The reductions in these indicators were more pronounced in the Baicalein+TAK242 group compared to the Baicalein or TAK242 groups alone. The brain structure and the number of nerve cells were partially restored in the Baicalein and TAK242 groups compared to the PCIV group, and this restoration was more evident in the Baicalein+TAK242 group. The number of nerve cells in the Baicalin+TAK242 group were significantly reduced.</p><p><strong>Conclusion: </strong>Baicalein alleviates vertigo symptoms, improves vestibular nucleus blood circulation, inhibits inflammatory responses, and reduces brain tissue damage in PCIV rats, at least in part through inhibition of the TLR4/NF-κB pathway.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Anti-Glioblastoma Effects of Magnolol Are Mediated through Paradoxical Enhancement of Antioxidant Defenses and Induction of Apoptosis.","authors":"Mohammad Zand, Seyyed Amirhossein Latifi, Mehdi Salehi, Hadi Karami","doi":"10.22074/cellj.2026.2072455.1932","DOIUrl":"https://doi.org/10.22074/cellj.2026.2072455.1932","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the antitumor effects of magnolol on T98G glioblastoma multiform cells and explored its underlying mechanisms, with a focus on oxidative stress modulation.</p><p><strong>Materials and methods: </strong>In this experimental study, cytotoxicity (MTT assay), proliferation (trypan blue), migration (wound healing), clonogenicity (colony formation), and apoptosis (Hoechst 33342) were assessed. Gene expression of antioxidant enzymes [superoxide dismutase (<i>SOD</i>), catalase (<i>CAT</i>), glutathione peroxidase (<i>GPX</i>)], apoptotic (<i>BAD, SURVIVIV</i>), cell cycle (<i>CYCLIN D1</i>), and metastatic (<i>MMP-9</i>) markers was evaluated viareverse transcription quantitative polymerase chain reaction (RT-qPCR). Antioxidant enzyme activity and total antioxidant capacity (TAC) were measured using commercial kits.</p><p><strong>Results: </strong>Magnolol dose-dependently suppressed cell viability (IC₅₀=72.39 μM), proliferation, migration, and colony formation. It induced apoptosis, upregulated Bad, SOD, CAT, and GPX expression, and downregulated survivin, cyclin D1, and MMP-9. Crucially, magnolol significantly elevated SOD, CAT, and GPX activities, thereby enhancing total antioxidant capacity.</p><p><strong>Conclusion: </strong>Magnolol exerts potent anti-glioblastoma effects by inducing a paradoxical hyper-activation of the antioxidant system, disrupting pro-survival redox signaling, and triggering apoptosis. This novel mechanism highlights its potential as a therapeutic agent.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of An Optimized Collagen Extraction Protocol forRegenerative Medicine Application: A Comparative Studyof Eleven Methods.","authors":"Tayebeh Sadat Tabatabai, Arian Ehterami, Tahereh Sadat Tabatabai, Majid Salehi","doi":"10.22074/cellj.2026.2076915.1966","DOIUrl":"https://doi.org/10.22074/cellj.2026.2076915.1966","url":null,"abstract":"<p><strong>Objective: </strong>Collagen plays a critical role in tissue engineering and regenerative medicine due to its biocompatibility and structural features. Identifying an optimal extraction method that preserves both functional and structural integrity remains challenging. This study aimed to compare eleven collagen extraction protocols to determine the most effective method for producing high-quality collagen.</p><p><strong>Materials and methods: </strong>In this a comparative experimental study, collagen was extracted using eleven protocols and characterized using scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), XRD, Raman spectroscopy, MTT and anti-inflammatory assays, lactate dehydrogenase (LDH) cytotoxicity, total antioxidant capacity (TAC), blood compatibility tests, protein quantification (BCA and Bradford), sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and solubility evaluations at varying pH and salt concentrations. The most promising sample was further evaluated <i>In vivo</i> via subcutaneous transplantation in mice, followed by histological analysis at 30 days.</p><p><strong>Results: </strong>Among the evaluated protocols, protocol 4 consistently exhibited superior performance, yielding approximately 35 mg collagen per g tissue. SEM, FTIR, XRD, and Raman analyses confirmed preserved triple-helical structure and fiber morphology. Protocol 4 showed high cell viability, low cytotoxicity, optimal blood compatibility, significant antioxidant activity, and improved protein content (P<0.05). <i>In vivo</i> studies demonstrated normal tissue regeneration, including collagen deposition, angiogenesis, and epidermal restoration, without inflammatory response. Other samples exhibited incomplete structural features, reduced yield, or increased cytotoxicity.</p><p><strong>Conclusion: </strong>Protocol 4 provides a robust approach for extracting collagen with high structural integrity, functional stability, and excellent biocompatibility. This optimized collagen may be suitable for biomedical applications and could support further translational research for collagen-based therapeutic products.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-18"},"PeriodicalIF":1.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Converging Advances in Reproductive Biomedicine and Stem CellTechnology: Reflections from The 25th and 20thRoyan Congresses: A Commentary.","authors":"Samaneh Adhami, Mahsa Sheikhan, Zeinab Ghezelayagh, Rouhollah Fathi, Maryam Hafezi, Parvaneh Afsharian, Abdolhossein Shahverdi, Leila Satarian, Leila Taghiyar","doi":"10.22074/cellj.2026.2084389.2006","DOIUrl":"https://doi.org/10.22074/cellj.2026.2084389.2006","url":null,"abstract":"<p><p>The 25^th International Congress on Reproductive Biomedicine and the 20^th International Congress on Stem Cell Biology and Technology, held in Iran, brought together leading global experts to discuss pioneering advances in stem cell research and reproductive medicine. Key topics included recent progress in somatic and pluripotent stem cells toward clinical applications, developments in regenerative medicine for diverse diseases, innovations in tissue engineering, the integration of artificial intelligence (AI) in biology research, and novel strategies for treating infertility. The congresses fostered collaboration and knowledge exchange across these rapidly evolving fields, highlighting the transformative potential of stem cells in regenerative medicine and their applications in reproductive health. Participation of 33 scientists from the United States, the United Kingdom, Germany, Austria, Italy, Belgium, Turkey, China, Russia, Japan, Sweden, Switzerland, Qatar, and India facilitated a rich exchange of ideas and broadened international perspectives. The insights and outcomes from these congresses are expected to shape ongoing research initiatives and clinical practices worldwide, emphasizing the importance of continued investment in these critical areas of medicine.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"28 1","pages":"1-7"},"PeriodicalIF":1.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Efficacy of Adipose-Derived Stem Cells for Bleomycin-Induced Idiopathic Pulmonary Fibrosis: A Comparative Study of Intravenous versus Intratracheal Administration.","authors":"Yan-Yan Ng, Chen-Yu Chiang, Yung-Lun Ni, Huan-Ting Shen, Chien-Ying Lee, Tai-Ping Lee, Martin Sieber, Ching-Chi Tseng, Thomas Chang-Yao Tsao, Yu-Hsiang Kuan","doi":"10.22074/cellj.2026.2066514.1886","DOIUrl":"https://doi.org/10.22074/cellj.2026.2066514.1886","url":null,"abstract":"<p><strong>Objective: </strong>To compare the therapeutic effects of intravenous (IV) and intratracheal (IT) delivery of adipose-derived stem cells (ADSCs) in a mouse model of bleomycin (BLM)-induced idiopathic pulmonary fibrosis (IPF).</p><p><strong>Materials and methods: </strong>In this experimental study, BALB/c mice were divided into 4 groups: control, BLM only, BLM+ADSC(IV), BLM+ADSC(IT). ADSCs were administered on day 14 after BLM induction. Outcomes measured on day 42 included mortality, body weight, lung edema, collagen deposition, fibrosis scoring, cytokine levels (TGF-β, SDF-1, IL-6, IL-1β, TNF-α), hydroxyproline, and leukocyte infiltration.</p><p><strong>Results: </strong>ADSC(IT) reduced mortality more effectively than ADSC(IV). Both IV and IT mitigated weight loss and lung edema with no significant difference. Both routes significantly decreased collagen synthesis, collagen fiber accumulation, Ashcroft scores, and hydroxyproline levels. Both routes lowered TGF-β, α-SMA, TNF-α, IL-6, IL-1β, and SDF-1 levels, and reduced leukocyte infiltration and alveolar barrier dysfunction, with no significant difference between routes.</p><p><strong>Conclusion: </strong>This design enables discrimination between route-dependent and route-independent therapeutic ef fects of ADSCs, thereby providing new insight into the optimization of stem cell delivery strategies for pulmo nary fibrosis. Importantly, whether the choice of deliv ery route differentially affects survival and inflammatory resolution, independent of antifibrotic efficacy, remains insufficiently addressed in current preclinical studies.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of Pparγ target genes in testis of rats under theinfluence of paternal trans fatty acid and vitamin-E.","authors":"Soheil Vesal, Danial Hashemi Karoii, Amir Abbas Shams, Raha Favaedi, Samira Vesali, Alireza Alizadeh, Maryam Shahhoseini","doi":"10.22074/cellj.2026.2050175.1770","DOIUrl":"https://doi.org/10.22074/cellj.2026.2050175.1770","url":null,"abstract":"<p><strong>Objective: </strong>A paternal high-fat diet (HFD) has been shown to affect the expression of peroxisome proliferator-activated receptors (<i>Ppars</i>), particularly in offspring's testicle regions. One of its isoforms, <i>Pparγ</i>, participates in spermatogenesis as a transcription factor regulating fatty acid metabolism genes. This study aimed to investigate how paternal dietary intake of trans fatty acids and/or vitamin E influences the expression of Pparγ target genes in the testes of their offspring, with the goal of elucidating potential mechanisms related to altered lipid metabolism and reproductive health.</p><p><strong>Materials and methods: </strong>In this experimental study, adult male Wistar rats (F0) were fed for 60 days with one of the following four diets: control diets (C), control diets with trans fatty acids (CTH), diets containing vitamin E (E) and diets containing vitamin E and trans fatty acids (ETH). Then the male offspring (F1) were raised on standard chow, and their testicular tissue was later analyzed to assess the impact of paternal diet on gene expression, and the expression of the <i>Pparγ</i> target genes: <i>Elovl2, Muc1, Fads2, Scd1, Glut2</i> and <i>Lpl</i> were measured quantitatively.</p><p><strong>Results: </strong>The data revealed that paternal HFD can suppress the expression of <i>Pparγ</i> target genes in the testes of offspring. <i>Fads2, Elovl2</i> were significantly upregulated in response to paternal vitamin E supplementation. The changes in Lpl gene expression were not statistically significant. Also, co-expression analysis and functional enrichment approach indicated that the genes involved in cellular response to fatty acid, fatty acid metabolic process, lipid storage, and fatty acid biosynthetic process were overexpressed in up/down regulated <i>Pparγ</i> target genes.</p><p><strong>Conclusion: </strong>Our findings demonstrate the metabolic impact of paternal diet on offspring's, with focus on mechanisms related to altered lipid metabolism and reproductive health.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Daucus carota and Piper nigrum Extracts against Methotrexate-Induced Testicular Damage in Rats.","authors":"Fatemeh Taleahmad, Mohsen Khalili, Narges Haddadzadeh-Niri, Ensiyeh Joneidi, Sara Taleahmad, Hasti Jabbari, Mehrdad Roghani","doi":"10.22074/cellj.2026.2049992.1769","DOIUrl":"https://doi.org/10.22074/cellj.2026.2049992.1769","url":null,"abstract":"<p><strong>Objective: </strong>This research aimed to investigate the effects of alcoholic extracts from <i>Daucus carota</i> and <i>Piper nigrum</i> on testicular damage induced by methotrexate (MTX) in rats.</p><p><strong>Materials and methods: </strong>In this experimental study, a total of 49 rats were randomly divided into seven groups, receiving MTX (20 mg/kg) alone and in conjunction with different doses of <i>Daucus carota</i> extract (200 and 400 mg/kg), <i>Piper nigrum</i> extract (15 and 7.5 mg/kg), a combination of <i>Pipe nigrum</i> at 7.5 mg/kg, and <i>Daucus carota</i> at 200 mg/kg, and a control group. The extracts were given orally through gavage starting one day before MTX treatment and continuing for two weeks. The study measured blood testosterone levels, Johnson's index, and seminiferous tubule diameter from tissue sections. It also assessed markers of oxidative stress [reactive oxygen species (ROS), Nitrite, malondialdehyde (MDA), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), as well as levels of myeloperoxidase (MPO), mitochondrial membrane potential (MMP), and the consistency of testicular tissue.</p><p><strong>Results: </strong>The MTX-exposed group exhibited remarkable testicular tissue damage, as indicated by increased ROS, MDA, and Nitrite levels compared to the control group. Moreover, there were notable reductions in CAT, GSH, SOD, testosterone levels, Johnson index, and seminiferous tubule diameter in the MTX-treated rats. However, treatment with <i>Pipe nigrum</i> and the combination of <i>Pipe nigrum</i> with Daucus carota extracts significantly reduced testicular damage and reduced oxidative stress markers compared to the MTX group.</p><p><strong>Conclusion: </strong>The study suggests that alcoholic extracts of <i>Daucus carota</i> and <i>Pipe nigrum</i> may help alleviate the harmful effects of MTX on testicular tissue, potentially offering a therapeutic option for male infertility due to chemotherapyrelated testicular damage.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}