PLoS Neglected Tropical Diseases最新文献

{"title":"The effects of mating and blood feeding on the immune defense of female Aedes aegypti mosquitoes.","authors":"Brendan J Kelly, Nicki C Joseph, Dom Magistrado, Sarah M Short","doi":"10.1371/journal.pntd.0013542","DOIUrl":"10.1371/journal.pntd.0013542","url":null,"abstract":"<p><p>Mating and consequent reproduction have variable effects on the immune defenses of female insects, ranging from immune enhancement to immune suppression. Blood feeding, which is necessary to induce egg production in anautogenous insect species, also impacts immunity, with variable effects in different insect species. Because immunity is a key component of vector competence, and mating and blood feeding can influence immunity, we investigated the effects of these processes on immune defense in the yellow fever mosquito, Aedes aegypti. To explore both aspects of reproduction, we measured survival, bacterial load, and bacterial prevalence in response to mating and blood feeding in adult female Ae. aegypti infected with various doses of the opportunistic bacterial pathogen, Serratia marcescens. When we challenged females with a high dose of S. marcescens, we found that mating induced significantly higher survival probabilities and bacterial loads in non-blood fed individuals. Mating improved survival after infection with a moderate dose of S. marcescens as well, but when we challenged females with a low dose, we observed no effect of mating on survival or bacterial load. Thus, the effects of mating on immune defense appear to be dose dependent. Blood feeding, on the other hand, consistently reduced survival regardless of dose. By exploring how key life history traits impact immune defense, our results continue to advance a thorough understanding of how Ae. aegypti defend against infection.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013542"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable digital microscopy with point-of-care testing for low-cost and efficient prevalence surveys for schistosomiasis control.","authors":"Isaac I Bogoch, Jean T Coulibaly, Kigbafori D Silue, Karla N Fisher, María Díaz de León Derby, Daniel A Fletcher, Nathan C Lo","doi":"10.1371/journal.pntd.0013444","DOIUrl":"10.1371/journal.pntd.0013444","url":null,"abstract":"","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013444"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital transmission of Chagas disease by vector circulation zone in Bolivia.","authors":"Beatriz Amparo Rodríguez, Freddy Tinajeros, Beth J Condori, Melissa Klein Cutshaw","doi":"10.1371/journal.pntd.0013591","DOIUrl":"10.1371/journal.pntd.0013591","url":null,"abstract":"<p><strong>Background: </strong>Bolivia has one of the highest burdens of Chagas disease. Transmission is most common through the triatomine bug vector carrying Trypanosoma cruzi infection, but a rising proportion of cases occurs through congenital transmission from mother to infant. Women living in endemic regions with high vector circulation are known to have an elevated risk of Chagas disease, but the relative risk of congenital transmission is unclear.</p><p><strong>Methods: </strong>We performed a prospective observational study of pregnant women with Chagas disease and their infants at 11 hospitals in Bolivia from September 2020 to March 2023. High vector circulation zones were defined as having triatomine infestation in >3% of local homes. Congenital Chagas disease was diagnosed in infants using polymerase chain reaction (PCR) at birth, micromethod at birth and 1 month, or serology at 9 months.</p><p><strong>Results: </strong>We enrolled 238 pregnant women, with a mean age of 28.7 years; 139 (58.4%) lived in high vector circulation areas. Of these, 19 women delivered infants who tested positive for T. cruzi infection (transmission rate 8.0%). Infants with congenital Chagas disease were significantly more likely to require hospitalization after birth (21.1% vs. 5.8%, p = 0.013). Women living in high vector circulation areas were more likely to have homes with mud walls (p < 0.001) and thatched roofs (p < 0.001) and to report having seen triatomine bugs in their home (p = 0.001). Congenital transmission rates did not significantly differ between women from low or high vector circulation zones (10.1% [9 of 139] vs. 6.5% [10 of 99], p = 0.31).</p><p><strong>Conclusions: </strong>Congenital transmission of Chagas disease remains common through multiple regions of Bolivia, regardless of local vector circulation control, and is associated with markedly higher rates of infant hospitalization after birth.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013591"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe dengue in adults: Clinical features from the 2022 dengue outbreak at a Vietnamese tertiary hospital.","authors":"Oanh Kim Pham, Thuy Bich Duong, Tho Vinh Phan, Trung Ngoc Truong, Duong Thi Hai Ha, Hao Van Nguyen, Vu Thi Minh Hien, Trinh Huu Khanh Dong, Nguyen Lam Vuong, Lam Minh Yen, Sophie Yacoub, Catherine Louise Thwaites, Dong Thi Hoai Tam","doi":"10.1371/journal.pntd.0013589","DOIUrl":"10.1371/journal.pntd.0013589","url":null,"abstract":"<p><strong>Background: </strong>In Vietnam, dengue has been endemic for many years, with most cases reported in children. Recently, epidemiological data show an increasing frequency in adults, especially for severe dengue. An unprecedented post-COVID-19 surge resulted in an exceptionally high number of hospitalized dengue cases. We aim to describe the clinical phenotypes and outcomes in Vietnamese adults with severe dengue during the 2022 outbreak and explore host-related factors associated with disease variability and severity, through a retrospective study.</p><p><strong>Findings: </strong>A total of 891 cases were included, with mean age 29 ± 10 years. 284/891 (31.9%) patients had a BMI ≥ 25 kg/m2, and 240/891 (26.9%) had comorbidities. The predominant severe clinical phenotype was dengue shock syndrome (DSS): 737/891 (82.7%) patients. 107/891 (12%) DSS cases were associated with other severe manifestations. Severe hemorrhage accounted for 90/891 (10.1%) patients. Among cases with organ involvement (211/891 - 23.7%), hepatic impairment was observed in 196/891 (22%) patients, renal impairment 25/891 (2.8%), cardiac impairment 14/891 (1.6%) and neurological impairment 13/891 (1.5%). 250/737 (33.9%) DSS patients developing ≥ 1 episode of recurrent shock. They were younger than those without recurrent shock (25.3 vs 28.4 years, p = 0.007). Factors associated with recurrent shock episodes were: having BMI ≥ 25 (OR: 1.65; 95% CI: 1.18; 2.3), day of illness ≤ 5 (OR: 2.16; 95% CI: 1.51; 3.09) and prior COVID-19 infection (OR: 2.57; 95% CI: 1.62-4.06). Indicators for the \"associated severe phenotypes\" (DSS associated with severe hemorrhage, with organ impairment, or both) were older age (p = 0.018) and presence of comorbidities (p < 0.001) compared to the DSS alone phenotype. Overall, 98.1% of patients had a good recovery.</p><p><strong>Conclusions: </strong>Understanding the variability and complexity of severe dengue clinical manifestations, along with the different host factors associated with these features, will contribute to formulating suitable treatment guidelines for this at-risk population.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013589"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and laboratory profiles of Oropouche virus disease from the 2024 outbreak in Manaus, Brazilian Amazon.","authors":"Maria Paula Gomes Mourão, Gisely Cardoso de Melo, Joabi Nascimento, Victor Irungu Mwangi, Livia Sacchetto, Luiz Gustavo Gardinassi, Rebeca Linhares Abreu Netto, Jady Mota, Sergio Damasceno Pinto, Mayara Tavares, Liz Moreira Cordeiro, Barbara Seffair de Castro, de Abreu Brasil, Dyandra Dos Santos Porto, Bianca Daniele Silva de Oliveira, Ana Carolina Shuan Laco, Lorenna Pereira de Souza, Karolaine Oliveira Bentes, Marcia Castilho, Carlos Eduardo Morais, Heline Silva Lira, Luís Felipe Alho, Flor Ernestina Martínez Espinosa, Vanderson Sampaio, Felipe Murta, Marco Aurélio Sartim, Maurício Lacerda Nogueira, Marcus Lacerda, Wuelton Monteiro","doi":"10.1371/journal.pntd.0013604","DOIUrl":"10.1371/journal.pntd.0013604","url":null,"abstract":"<p><strong>Background: </strong>The 2024 Oropouche virus (OROV) outbreak in Brazil raised public health concerns due to its unprecedented rapid spread, high incidence, and potential neurological complications. OROV symptoms overlap with locally endemic arbovirus diseases, like dengue virus (DENV), complicating diagnosis. The study aimed to compare clinical, laboratory, and immunological profiles in OROV and DENV cases, crucial for improving diagnosis and management.</p><p><strong>Methods: </strong>This study analyzed 51 OROV and 78 of DENV cases consecutively enrolled in Manaus, Amazonas, Brazil, and monitored for 28 days. OROV diagnosis was performed by real-time PCR (RT-PCR) using serum and urine samples. OROV RT-PCR positive samples were genotyped. A paired Plaque Reduction Neutralization Test (PRNT) was conducted on samples collected at D1 and D28. Patients with a ≥ 4-fold increase in neutralizing antibody titer between D1 and D28 were considered OROV-positive. Clinical manifestations, hematology, biochemistry, and cytokine profiles were analyzed. Statistical analysis included comparison between OROV and DENV patients.</p><p><strong>Results: </strong>Genome sequencing of OROV isolates confirmed presence of a previously reported novel reassortment event, consistent with ongoing localized transmission. Urine RT-PCR demonstrated low positivity compared to serum samples. The paired PRNT increased sensitivity in 45%. Clinically, OROV infection was associated with significantly higher frequencies of severe headache, myalgia, arthralgia, and rash compared to DENV infection (p < 0.001). Elevated alanine aminotransferase (ALT) levels were also observed in OROV patients (p < 0.001). Immunologically, OROV infection induced significantly increased levels of acute-phase CCL11 (eotaxin), CXCL10, IFN-γ, IL-1RA, and IL-10, which declined by day 28, while IL-5 increased during recovery. In contrast, DENV patients exhibited elevated levels of CCL2, G-CSF, and CCL3 in recovery phase.</p><p><strong>Conclusion: </strong>OROV symptoms overlap with DENV underscores the need for syndromic diagnostic approach in endemic regions. Continued genomic surveillance and expanded clinical studies are vital to assess long-term consequences. Given OROV's expanding geographic range, targeted public health measures are essential to mitigate future outbreaks and better understand its pathophysiology.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013604"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and spatial profiling of Aedes albopictus immune responses to chikungunya virus infection.","authors":"Maria Greta Dipaola, Claudia Fortuna, Francesco Severini, Giulia Bevivino, Marco Di Luca, Tony Nolan, Marco Salvemini, Bruno Arcà, Fabrizio Lombardo","doi":"10.1371/journal.pntd.0013588","DOIUrl":"10.1371/journal.pntd.0013588","url":null,"abstract":"<p><p>The global expansion of Aedes albopictus from Southeast Asia to various regions worldwide poses a significant public health concern due to its role as a vector for several pathogens, including chikungunya virus (CHIKV), which infects over one million people annually. In this study, aimed at understanding the molecular interactions between Ae. albopictus and CHIKV, we analyzed by RNA sequencing CHIKV-infected and uninfected control mosquitoes. We focused our attention on key mosquito organs at one- and five-days post-blood meal ingestion, which correspond to viral entry and dissemination, and found specific transcriptional changes involving various pathways during the CHIKV lifecycle. The mosquito midgut plays a crucial role in the early stages, when the virus enters along with human blood components, encounters the resident microbiota, interacts with the developing peritrophic matrix, and counteracts the mosquito's digestive enzymes. We found that RNA interference (RNAi) was predominantly activated in the midgut during the initial virus invasion. Additionally, several key enzymes involved in autophagy and ubiquitination were also more abundant in infected midguts compared to controls. At later time points, after viral dissemination into the hemocoel, key immune responses are triggered in the hemolymph and, accordingly, immune mechanisms such as the activation of leucine-rich repeats (LRRs) proteins, secretion of antimicrobial peptides (e.g., holotricin), and melanization (mediated by phenoloxidase, PO) were the most prominent. RNA-seq results were validated by RT-qPCR on selected candidates in different tissues and a catalogue of Ae. albopictus immune genes (891 contigs) grouped into 24 different immune and immune-related families was compiled. This study explores the molecular interactions between Ae. albopictus and CHIKV across developmental stages, providing key insights into arbovirus transmission dynamics and mosquito vector competence.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013588"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The probability of Plasmodium vivax acute illness following primary infection and relapse in Papua New Guinea.","authors":"Amanda Ross, Cristian Koepfli, Lincoln Timinao, Diggory Hardy, Tobias Thüring, Melanie Loeffel, Benson Kiniboro, Ingrid Felger, Ivo Mueller","doi":"10.1371/journal.pntd.0013567","DOIUrl":"10.1371/journal.pntd.0013567","url":null,"abstract":"<p><p>Inoculation with Plasmodium vivax malaria parasites can lead to blood-stage infections from the primary infection and relapses from liver-stage parasites or non-circulating merozoites. Understanding the risk of clinical illness following primary infection and relapse would inform surveillance and intervention strategies, but the probabilities are uncertain in people living in endemic areas. A major difficulty lies in the inability to distinguish primary infections and relapses. In this study, we estimate the probabilities of clinical illness using the different seasonal patterns of primary infection and relapse. Children aged one to three years in Ilaita, Papua New Guinea, were followed up over 16 months for illness (fever with ≥500 parasites/µl) with fortnightly active and passive case detection, and for blood-stage infection every two months. Estimates of the number of primary infections and relapses for each two-month time-period, age-group, village and ITN use category were derived from previous analyses using genotyping data. In this study, we use a Bayesian statistical model to relate the number of observed P. vivax clinical cases in each covariate category to the expected numbers of primary infections and relapses. We include the cumulative number of primary infections experienced since birth as a proxy for acquired immunity. To reflect uncertainty, we use varying assumptions about whether relapses can cause illness in different circumstances. The probability of illness decayed exponentially with increasing cumulative numbers of primary infections experienced. The estimated probability of illness following relapses was lower than that for primary infection, how much lower depended on how they were defined. Later relapses within the same brood tended to have lower probabilities than earlier ones. Varying seasonally, relapses were estimated to contribute half of P. vivax illness in this cohort despite accounting for 80% of the force of blood-stage infection. The results can inform estimates of the burden of P. vivax and provide building blocks for mathematical models for predicting the impact of interventions. Interventions triggered by clinical cases would focus on more recent infections and age-groups with less acquired immunity.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 10","pages":"e0013567"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixing methods: How listening to researchers, healthcare workers, and community members can improve the design of studies testing medical innovations for Neglected Tropical Diseases.","authors":"Astrid C Erber, Anthony Ablordey, Adamu Addissie, Evans K Ahortor, Doris Burtscher, Esther M Mukooza, Richard Creswell, Ben Lambert, Piero L Olliaro","doi":"10.1371/journal.pntd.0013547","DOIUrl":"10.1371/journal.pntd.0013547","url":null,"abstract":"","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 9","pages":"e0013547"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and practices on anthrax in selected game management areas in Zambia.","authors":"Exillia Kabbudula, Laila Gondwe, Chitwambi Makungu, Mtui-Malamsha N Jesse, Kezzy Besa, Suwilanji S Sichone, Noanga Mebelo, Mwila Kayula, Geoffrey Mainda, Fredrick M Kivaria, Charles Bebay, Baba Soumare, Suze P Filippini, Chisoni Mumba","doi":"10.1371/journal.pntd.0013592","DOIUrl":"10.1371/journal.pntd.0013592","url":null,"abstract":"<p><p>In this study, we investigated anthrax, a zoonotic disease, at the human-wildlife-livestock interface in Zambia, focusing on Simalaha Conservancy, Blue Lagoon, and Lochinvar National Parks. These areas represent key points where illegal wildlife trade and anthrax risk coexist. Although anthrax remains endemic in Zambia, there is limited data on community knowledge, attitudes, and practices (KAPs) related to anthrax transmission and the role of wildlife trade value chains in its spread and maintenance. Therefore, we examined how these community KAPs influence anthrax transmission through the illegal game meat trade. We conducted a cross-sectional study involving 1,187 participants and analyzed data using descriptive statistics and logistic regression. Our findings revealed significant variations between knowledge of anthrax and behavior. While more than 80% of respondents demonstrated positive attitudes and safe practices, their knowledge of anthrax remained limited. Specifically, 72.5% of participants had low awareness of the disease, yet 82.1% acknowledged its severity, and 59.4% recognized vaccination as a control measure. Despite these positive attitudes, misconceptions about anthrax transmission and treatment were widespread. Some community members relied on spiritual or herbal remedies rather than seeking medical intervention. We also identified several risky behaviors that contribute to anthrax transmission. Notably, 14.9% of respondents admitted to consuming meat from animals that had died suddenly, while 46.3% reported handling potentially infected carcasses or animal products without taking safety precautions. We found strong correlations between knowledge, attitudes, practices, and education levels. Our results showed that prior exposure to anthrax information was the strongest predictor of knowledge (β > 1). Our findings highlight the urgent need for targeted public health interventions, improved veterinary services, and stricter enforcement of wildlife trade regulations to reduce the risk of anthrax transmission in these communities.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 9","pages":"e0013592"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of panton valentine leukocidin-producing Staphylococcus aureus infections, Djibouti, 2018-2023.","authors":"Maxime Danjean, Virginie Courbin, Aurore Ho, Aurore Bousquet, Christophe Rodriguez, Hervé Jacquier, Paul-Louis Woerther","doi":"10.1371/journal.pntd.0013544","DOIUrl":"10.1371/journal.pntd.0013544","url":null,"abstract":"<p><p>Between 2018 and 2023, a genomic study was conducted in a military camp in Djibouti to investigate the molecular epidemiology of Panton-Valentine Leukocidin-producing Staphylococcus aureus. Among 43 isolates, Sequence Type 152 was predominant (72%), mainly associated with spa types t355 and t4235. Core-genome Multi-Locus Sequence Typing revealed two concurrent transmission dynamics: localized inter-human outbreaks and repeated introductions from external sources. Comparative genomics with other African Sequence Type 152 isolates showed similar levels of diversity, suggesting a widespread continental dissemination. These findings highlight the importance of genomic surveillance to better understand and control the spread of this virulent lineage in Africa.</p>","PeriodicalId":49000,"journal":{"name":"PLoS Neglected Tropical Diseases","volume":"19 9","pages":"e0013544"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}