New Phytologist最新文献_第6页

Tropism dynamics in plant movement: insights into the mechanism of proprioception sensing in wood architecture and stem posture control in trees. 植物运动中的向性动力学：对木结构本体感觉感知和树木茎位控制机制的见解。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-02 DOI: 10.1111/nph.71436

Pramod Sivan

引用次数: 0

Tree mycorrhizal types differentially mediate soil organic carbon and its fractions at the aggregate scale: key roles of metal elements and microbial traits. 树木菌根类型在团聚体尺度上对土壤有机碳及其组分的调节差异：金属元素和微生物性状的关键作用。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-14 DOI: 10.1111/nph.71357

Qianru Ji, Haitong Liu, Mingshan Zhang, Jiazeng Song, Xue Fang, Wenjie Wang, Huimei Wang

{"title":"Tree mycorrhizal types differentially mediate soil organic carbon and its fractions at the aggregate scale: key roles of metal elements and microbial traits.","authors":"Qianru Ji, Haitong Liu, Mingshan Zhang, Jiazeng Song, Xue Fang, Wenjie Wang, Huimei Wang","doi":"10.1111/nph.71357","DOIUrl":"10.1111/nph.71357","url":null,"abstract":"Aggregates, fundamental structural units of soils, significantly affect soil organic carbon (SOC) dynamics. Yet, how tree mycorrhizal types drive SOC dynamics at the aggregate scale remains unclear. We investigated the impacts of three arbuscular mycorrhizal (AM) and three ectomycorrhizal (ECM) tree species on SOC and its particulate fractions, and the roles of metal elements and microbial communities across aggregate size classes in a temperate forest. AM tree soils exhibited higher macroaggregate proportions and aggregate stability, as well as increased contents of SOC, particulate organic carbon (POC), and mineral-associated organic carbon (MAOC) across all aggregate size fractions than those under ECM trees. AM tree soils were richer in Ca, Mg, Fe, and Al, whereas ECM tree soils had higher K and Na. Mycorrhizal types and aggregate size (macroaggregates vs microaggregates) directly or indirectly affected SOC fractions through soil metal elements, total nitrogen (TN), fungal richness, and Bacteroidota abundance. Our study demonstrates that AM and ECM trees influence SOC dynamics through aggregate-size-dependent changes in soil TN, metal elements, and microbial traits, and that Ca, fungal richness, and Bacteroidota are particularly influential mediators. These findings underscore the importance of incorporating tree mycorrhizal traits into SOC dynamics under global change.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2948-2963"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 3D genome of Gigaspora margarita unveils stable chromatin and nucleolar organization and symbiont-dependent genome dynamics. 玛格丽塔线虫的三维基因组揭示了稳定的染色质和核仁组织和共生体依赖的基因组动力学。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-03-20 DOI: 10.1111/nph.71100

Ken Mugambi, Jordana Oliveira, Franco Magurno, Alessandra Salvioli di Fossalunga, Mara Novero, Luisa Lanfranco, Stefano Ghignone, Gökalp Yildirir, Yan Wang, Paola Bonfante, Nicolas Corradi

{"title":"The 3D genome of Gigaspora margarita unveils stable chromatin and nucleolar organization and symbiont-dependent genome dynamics.","authors":"Ken Mugambi, Jordana Oliveira, Franco Magurno, Alessandra Salvioli di Fossalunga, Mara Novero, Luisa Lanfranco, Stefano Ghignone, Gökalp Yildirir, Yan Wang, Paola Bonfante, Nicolas Corradi","doi":"10.1111/nph.71100","DOIUrl":"10.1111/nph.71100","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are widespread plant symbionts that enhance nutrient acquisition and influence ecosystem productivity. Previous chromosome-level assemblies of the model species Rhizophagus irregularis revealed a two-compartment genome architecture (active A and repressed B chromatin compartments), yet its conservation across evolutionarily distant AMF lineages remains unresolved. Here, we present a chromosome-scale and 3D genome assembly of Gigaspora margarita isolate BEG34 - the largest and most repeat-rich AMF genome to date - alongside that of its obligate endobacterium, Candidatus Glomerobacter gigasporarum (CaGg), using PacBio HiFi and Hi-C sequencing. The G. margarita genome comprises 43 chromosomes (792 Mb) organized into A/B compartments and Topologically Associating Domains, structures that are conserved across two AMF orders and remain stable irrespective of the presence of endobacteria in germinating spores. We uncover 21 divergent rDNA operons distributed across six chromosomes and show that these physically interact, suggesting conserved nucleolar organization. We also reveal that the CaGg genome is tripartite and mobilome-rich, encoding prophages, an orphan CRISPR array, and complete pathways for many novel and essential cofactors, including heme, which may enhance host bioenergetics. We also find that the endobacterium's presence modulates transposable elements expression in G. margarita. These findings reveal conserved principles of chromatin architecture in AMF symbionts and highlight the tight molecular interplay between fungal hosts and their endosymbionts, offering new insights into genome evolution and symbiotic adaptation.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2799-2817"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147488088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term effects of clear-cutting forestry on ectomycorrhizal fungi in boreal forest. 森林采伐对北方森林外生菌根真菌的长期影响。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-04-03 DOI: 10.1111/nph.71165

Björn D Lindahl, Karina E Clemmensen, Johan Stendahl, Anders Dahlberg

{"title":"Long-term effects of clear-cutting forestry on ectomycorrhizal fungi in boreal forest.","authors":"Björn D Lindahl, Karina E Clemmensen, Johan Stendahl, Anders Dahlberg","doi":"10.1111/nph.71165","DOIUrl":"10.1111/nph.71165","url":null,"abstract":"Clear-cutting is detrimental to ectomycorrhizal fungi in a short time perspective, but long-term effects on species richness and community composition are uncertain. To evaluate ecological sustainability of rotation forestry, we examined to what extent communities similar to those in old forests develop within the time frame of a rotation period. We conducted DNA-based analyses of ectomycorrhizal fungal communities in > 1500 sites across Swedish boreal forests and used space for time substitution to analyse the development of species richness, community composition, and abundance with increasing stand age, ranging from recent clear-cuts to 200-yr-old forests. Ectomycorrhizal fungal species richness peaked in 30- to 40-yr-old secondary stands, but community composition remained altered in up to 100-yr-old stands. Certain species in the Atheliaceae family thrived in secondary forests, whereas many Cortinarius and Russula species linked to acidic and unfertile soils were more common in old, natural forests. While Swedish rotation forestry does not appear to decrease species richness, it will progressively change the composition of ectomycorrhizal fungal communities at the landscape level, with species characteristic of acidic and nutrient-poor old-growth boreal forests decreasing in abundance.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2818-2831"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147617091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CrAGL62 and potential paternally expressed gene CrbZIP contribute to paternal-excess postzygotic barriers in citrus. CrAGL62和潜在的父本表达基因CrbZIP参与了柑橘父本合子后屏障过剩的产生。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-17 DOI: 10.1111/nph.71369

Jing-Jing He, Ming-Yao Shen, Gang Hu, Qiang-Ming Xia, Yao-Yuan Duan, Gong-Ao Xiao, Xiao-Meng Wu, Wen-Wu Guo, Kai-Dong Xie

{"title":"CrAGL62 and potential paternally expressed gene CrbZIP contribute to paternal-excess postzygotic barriers in citrus.","authors":"Jing-Jing He, Ming-Yao Shen, Gang Hu, Qiang-Ming Xia, Yao-Yuan Duan, Gong-Ao Xiao, Xiao-Meng Wu, Wen-Wu Guo, Kai-Dong Xie","doi":"10.1111/nph.71369","DOIUrl":"10.1111/nph.71369","url":null,"abstract":"Interploidy hybridization in plants is constrained by a postzygotic barrier. Triploid production via 2x × 4x interploidy hybridization is an important strategy for obtaining seedless cultivar in some fruit tree crops including citrus. However, as the molecular mechanisms of postzygotic barrier in citrus remain unclear, triploid breeding efficiency is limited. Here, we compared seed development in citrus 2x × 4x and 2x × 2x crosses. Embryo and endosperm developed normally in 2x × 2x crosses, while endosperm cellularization completely failed in 2x × 4x crosses, resulting in embryo arrest at the globular stage, demonstrating that impaired endosperm cellularization causes triploid embryo abortion. Transcriptomic analysis of laser microdissection (LMD)-captured endosperm at pre- and post-cellularization stages revealed extensive transcriptional misregulation in 2x × 4x crosses, including persistent upregulation of type I MADS-box genes (CrAGLs) and the potential paternally expressed gene CrbZIP. Consistently, functional analyses showed that overexpression of CrAGL62, CrAGL80a, or CrbZIP delayed endosperm cellularization in precocious Mini-citrus (Fortunella hindsii), while cragl62 mutants exhibited severely impaired cellularization. Our findings showed CrAGL62 and CrbZIP as key regulators of endosperm cellularization in citrus, providing potential targets for modulating endosperm development to improve citrus triploid breeding efficiency.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2604-2619"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A root-soil association index reveals life-history strategies of arbuscular mycorrhizal fungi. 根-土壤关联指数揭示了丛枝菌根真菌的生活史策略。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-04 DOI: 10.1111/nph.71338

Iñaki Odriozola, Felix Wesener, Petr Baldrian, Florian Barbi, Lukas Bell-Dereske, Karel Klem, Lenka Meszárošová, Otso Ovaskainen, Jakub Skřivánek, Tomáš Větrovský, Zuzana Kolaříková, Petr Kohout

{"title":"A root-soil association index reveals life-history strategies of arbuscular mycorrhizal fungi.","authors":"Iñaki Odriozola, Felix Wesener, Petr Baldrian, Florian Barbi, Lukas Bell-Dereske, Karel Klem, Lenka Meszárošová, Otso Ovaskainen, Jakub Skřivánek, Tomáš Větrovský, Zuzana Kolaříková, Petr Kohout","doi":"10.1111/nph.71338","DOIUrl":"10.1111/nph.71338","url":null,"abstract":"Arbuscular mycorrhizal (AM) fungal taxa differ in their life-history strategies, including their preferential association with intraradical vs extraradical compartments. We introduce the arbuscular mycorrhizal root-soil association index (AMF-RSI), a novel quantitative index describing the relative association of AM fungi with root vs soil compartments, corresponding to the previously proposed rhizophilic and edaphophilic AM fungi. The AMF-RSI was calculated from metabarcoding data compiled in the GlobalAMFungi database using Hierarchical Modeling of Species Communities and was subsequently applied to assess shifts in AM fungal community traits driven by soil disturbance caused by tillage. We assessed 301 AM fungal taxa and found that AMF-RSI was strongly conserved at the genus level. While the majority of AM fungal taxa from families Archaeosporaceae, Diversisporaceae and Paraglomeraceae exhibited preferential association with soil, taxa within Glomeraceae exhibited high variability in edaphophilic vs rhizophilic strategies. AMF-RSI values predicted AM fungal community responses to soil disturbance, with rhizophilic taxa being more prevalent in tilled soils. The AMF-RSI represents a new quantitative trait distinguishing preferential association of AM fungi with intraradical vs extraradical compartments (edaphophilic vs rhizophilic life-history strategies). Applied at the community level, it provides a tool for characterizing shifts in AM fungal communities in response to environmental conditions.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2932-2947"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148165526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A drought stress-induced MYB transcription factor regulates pavement cell shape in leaves of European aspen (Populus tremula). 干旱胁迫诱导的MYB转录因子调节欧洲白杨叶片的路面细胞形状。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-07-05 DOI: 10.1111/nph.71399

Sijia Liu, Siamsa M Doyle, Kathryn M Robinson, Zahra Rahneshan, Nathaniel R Street, Stéphanie Robert

{"title":"A drought stress-induced MYB transcription factor regulates pavement cell shape in leaves of European aspen (Populus tremula).","authors":"Sijia Liu, Siamsa M Doyle, Kathryn M Robinson, Zahra Rahneshan, Nathaniel R Street, Stéphanie Robert","doi":"10.1111/nph.71399","DOIUrl":"10.1111/nph.71399","url":null,"abstract":"Leaf pavement cells of many plant species develop jigsaw puzzle-like shapes in which neighboring cells interdigitate, providing an ideal model for the study of cell shape regulation. Such shapes are likely to reduce tissue-wide mechanical stress, which is influenced by environmental conditions, such as drought stress. We analyzed pavement cell shape complexity in a natural population of European aspen (Populus tremula) genotypes and used a genome-wide association study (GWAS) to identify a candidate gene in cell shape regulation, Potra2n8c18226, encoding the transcription factor MYB305a. We subsequently validated a role for MYB305a in regulating aspen leaf pavement cell shape. We then demonstrated that drought stress strongly induces MYB305a promoter expression in these cells and provided evidence that MYB305a plays a role in regulating pavement cell shape in response to drought. Finally, we observed correlations of pavement cell shape complexity with water-use efficiency and average precipitation at the original sampling sites, in the natural aspen population. Taken together, our results suggest climatic variables affect shape complexity of pavement cells in aspen leaf and provide a foundation for future mechanistic studies on this process, by implicating the involvement of the transcription factor MY305a.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2688-2705"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homology of the dark cells of Paleozoic liverworts with the specialized oil body cells of modern liverworts (Marchantiophyta). 古生代苔类植物暗细胞与现代苔类植物特化油体细胞的同源性。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-08 DOI: 10.1111/nph.71332

Susan Tremblay, Josep Mercadal

{"title":"Homology of the dark cells of Paleozoic liverworts with the specialized oil body cells of modern liverworts (Marchantiophyta).","authors":"Susan Tremblay, Josep Mercadal","doi":"10.1111/nph.71332","DOIUrl":"10.1111/nph.71332","url":null,"abstract":"Oil bodies are a synapomorphy of liverworts (Marchantiophyta), a major group of land plants with a sparse fossil record. Paleozoic liverworts sometimes possess dark cells that appear similar in distribution to liverwort oil body cells. The Middle Devonian Metzgeriothallus sharonae provides an opportunity for comparison with modern liverworts. Shale samples were collected, and the carbonaceous fossils were isolated by acid maceration. Museum shale specimens of Pallaviciniites devonicus were also obtained and processed. The relative location, frequency, and spatial distribution of the fossil dark cells were compared to those of oil body cells in extant taxa. Quantitative analyses revealed that the frequency and spatial distribution of dark cells are comparable to those of oil body cells. Microscopy results show evidence of oil body membranes within dark cells. The dark cells of M. sharonae show clumping near the thallus margin, suggesting an anti-herbivore function. These results support the hypothesis that the dark cells of Paleozoic liverworts and the oil body cells of extant lineages are homologous structures with a shared developmental origin, providing a new character that can aid in the classification of fossils and that sheds light on the evolution and function of liverwort oil bodies.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2995-3007"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148206637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root anatomical traits modulate the assembly and nitrogen-transformation potential of root-associated microbiomes in a temperate steppe. 温带草原根系解剖性状调节根系相关微生物组的组装和氮转化潜力。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-11 DOI: 10.1111/nph.71342

Guangyuan Yuan, Meng Zhou, Chao Xiong, Ren Bai, Fengfeng Cao, Yujia Yuan, Wen-Hao Zhang, Wenming Bai

{"title":"Root anatomical traits modulate the assembly and nitrogen-transformation potential of root-associated microbiomes in a temperate steppe.","authors":"Guangyuan Yuan, Meng Zhou, Chao Xiong, Ren Bai, Fengfeng Cao, Yujia Yuan, Wen-Hao Zhang, Wenming Bai","doi":"10.1111/nph.71342","DOIUrl":"10.1111/nph.71342","url":null,"abstract":"Plants have evolved numerous belowground strategies to capture nutrients. While root functional differentiation between absorption and transportation has been inferred from order-based traits, the role of microbiomes in mediating this differentiation remains unclear. We measured traits (anatomical, chemical, and morphological) of lower order (absorption-dominated) and higher order (transportation-dominated) roots of 37 herbaceous species (13 monocots and 24 dicots) in a temperate grassland. Furthermore, we employed high-throughput quantitative polymerase chain reaction and 16S rRNA gene sequencing to investigate bacterial nitrogen-transformation gene abundance, diversity, and community assembly along the soil-root continuum (rhizosphere, rhizoplane, and endosphere) and analyzed their relationships with root traits. Monocot roots exhibited greater bacterial diversity and nitrogen-transformation gene abundances than dicots. Within dicots, lower order roots showed higher bacterial diversity and nitrogen-transformation gene abundances than higher order roots, a pattern not observed in monocots. Lower order roots, characterized by higher cortex proportion, facilitated the enrichment of diverse bacteria and recruitment of nitrogen-transformation microorganisms. These patterns were associated with a decrease in homogeneous selection from lower order to higher order roots. This study reveals the mechanisms of functional differences among herbaceous root orders from a microbiome perspective, offering further insights into how root-microbe interactions underpin nitrogen-transformation potential in terrestrial ecosystems.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2497-2517"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-delayed activator-repressor regulation of ginsenoside biosynthesis in Gynostemma pentaphyllum. 绞股蓝人参皂苷生物合成的时滞激活-抑制因子调控。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-12 DOI: 10.1111/nph.71346

Lijin Huang, Jinfa Du, Yuhong Chen, Dian Jin, Yuyin Zhao, Xinyue Xing, Long Wang, Yingping Wang, Peina Zhou, Yucheng Zhao, Ping Li, Xu Lu

{"title":"Time-delayed activator-repressor regulation of ginsenoside biosynthesis in Gynostemma pentaphyllum.","authors":"Lijin Huang, Jinfa Du, Yuhong Chen, Dian Jin, Yuyin Zhao, Xinyue Xing, Long Wang, Yingping Wang, Peina Zhou, Yucheng Zhao, Ping Li, Xu Lu","doi":"10.1111/nph.71346","DOIUrl":"10.1111/nph.71346","url":null,"abstract":"Jasmonic acid (JA) signaling is a key regulator of plant secondary metabolism, yet how it balances rapid activation with timely repression of ginsenoside biosynthesis remains unclear in Gynostemma pentaphyllum. Here, we investigated the transcriptional regulation mechanism of ginsenoside biosynthesis by JA-responsive basic helix-loop-helix (bHLH) transcription factors. By integrating transcriptome and phylogenetic analyses with yeast one-hybrid, electrophoretic mobility shift assays, dual-luciferase assays, transgenic hairy root overexpression, and RNA interference, together with metabolite profiling, we identified key regulators and defined their roles in the ginsenoside biosynthesis pathway of G. pentaphyllum. We characterized an antagonistic bHLH pair, GpGAB (g36559, Activator), and GpGIB (g21771, Inhibitor). GpGAB activated ginsenoside biosynthetic genes GpDS, GpPPDS, and GpUGT94AT1, whereas GpGIB repressed the expression of GpDS and GpPPDS. GpGAB also upregulated the expression of GpGIB, with GpGAB responding earlier than GpGIB to JA treatment. Taken together, our results support a model in which GpGAB and GpGIB form a time-delayed negative feedback loop that fine-tunes ginsenoside biosynthesis after JA induction, providing a mechanistic framework for dynamic transcriptional regulation of ginsenoside in G. pentaphyllum.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2725-2740"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148240506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0