International Journal of Radiation Research最新文献

{"title":"Multislice CT combined with miR-let-7 for colon cancer diagnosis and miR-let-7 targeting HMGA2 to regulate cell cycle alterations in colon cancer cells","authors":"Kun Yang, Zheng Chen","doi":"10.61882/ijrr.23.3.9","DOIUrl":"https://doi.org/10.61882/ijrr.23.3.9","url":null,"abstract":"Background: Colon cancer (CC) is one of the most common malignancies in the digestive tract.In this study, we analysed the diagnostic value of multislice CT (MSCT) in combination with miR-let-7 in CC and the mechanism of action of miR-let-7 in CC. Materials and Methods:A total of 108 patients with suspected CC admitted to our hospital between March 2020 and May 2022 were retrospectively analysed; 37 patients were diagnosed with CC, and the remaining 71 were determined to have benign colonic lesions.To compare the diagnostic value of MSCT, miR-let-7, and MSCT combined with miR-let-7 detection for CC, we detected the expression of miR-let-7 and high mobility group AT hook protein 2 (HMGA2) in normal human colonic epithelial FHC cells and human colonic mucosal epithelial HCT116 cells and observed the effect of miR-let-7 on the biological behaviour of CC cells.Results: Compared with the results of pathological biopsy, the diagnostic sensitivity of MSCT combined with miR-let-7 for CC was 97.30%, the specificity was 98.59%, and the accuracy was 98.15%, with a misdiagnosis rate of only 1.85%.After miR-let-7 expression was silenced, the viability of colon cancer cells increased, and the inflammatory response and oxidative stress increased.In addition, there was a targeted regulatory relationship between HMGA2 and miR-let-7, which could reverse the effect of miR-let-7 on colon cancer cells.Conclusion: The combination of MSCT and the miR-let-7 assay is accurate for the diagnosis of CC, and miR-let-7 regulates the biological behaviour of CC cells through HMGA2.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"23 3","pages":"569-576"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1369-e1b68db.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary parotid gland malignant melanoma complicated with lung and bone metastasis; a case report","authors":"S Qin, Rui Li, Dan Zhou, Ming Li, Guangfu Li, Maxine Sun","doi":"10.61186/ijrr.23.2.36","DOIUrl":"https://doi.org/10.61186/ijrr.23.2.36","url":null,"abstract":"Background: Malignant melanoma of the primary parotid gland (PGMM) is extremely rare, and most of the previous reports are case reports.This study describes the clinical and histological characteristics of a single case of PGMM.Materials and methods: The patient was diagnosed following the detection of a mass in the parotid region.Examination of the resected tissue specimens demonstrated cells with copious eosinophilic cytoplasm, significant pleomorphic in the nuclei, and distinct nucleoli.Immunohistochemical analysis was performed to measure HMB45, S-100, SOX-10, and Melan-A markers expression in tumor tissue.The clinicopathological features of a series of parotid gland malignant melanomas were analyzed.Results: Despite undergoing surgical intervention, immunotherapy, and targeted therapy, this case occurred distant metastases.The patient survived 2 years and 3 months after surgery due to multiple metastases throughout the body.Conclusion: This article reported the clinical characteristics of the case and provided an overview of primary PGMM cases documented over the last three decades.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"23 2","pages":"509-512"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1359-3b774fa.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-18a-5p targets Sec61 translocon alpha 1 subunit to repress hepatocellular carcinoma cell growth before chromradiotherapy","authors":"Qian Chen, Mengmeng Wang, X-K. Meng, Qiong Wu","doi":"10.61186/ijrr.23.1.175","DOIUrl":"https://doi.org/10.61186/ijrr.23.1.175","url":null,"abstract":"Background: Hepatocellular carcinoma (HCC) cell development was investigated in relation to the regulation of Sec61 translocon alpha 1 subunit (SEC61A1) by microRNA (miR)-18a-5p.Materials and Methods: After collection of clinical samples, the transfection of interfering vectors of miR-18a-5p or SEC61A1 was into HCC cells to figure out their roles in development of HCC.The Pearson test and starBase analyzed the association and target prediction of miR-18a-5p.Subsequently, through the dual luciferase reporter experiment, SEC61A1 can be regulated via miR-18a-5p.The salvage experiment revealed that miR-18a-5p influence the degradation process of Hepatocellular carcinoma through combining SEC61A1.Results: In HCC cells, we found that SEC61A1 was elevated and miR-18a-5p was downregulated.HCC cells deteriorating was considerably slowed down by the enhanced miR-18a-5p level.The outcomes demonstrated that miR-18a-5p can interact and regulate SEC61A1.Additionally, the effects of earlier therapy on HCC cell proliferation can be restored by overexpressing SEC61A1.Conclusion: Overall, in HCC cells and tissues miR-18a-5p was significantly downregulated, and inhibited the proliferative ability of HCC cells by targeting SEC61A1.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"23 1","pages":"175-183"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1308-6accb39.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-intensity focused ultrasound inhibits lncRNA MAFA-AS1-modulated stability of HIF-1 mRNA in an m6A-dependent manner to suppress aerobic glycolysis and adriamycin resistance in ovarian cancer","authors":"M. Huang, Yuan Peng, Yuhui Sun, Wen Gao, J. Fei, Caicun Zhou, Li Xie, Li Zhou, Wei Wu","doi":"10.61186/ijrr.23.1.201","DOIUrl":"https://doi.org/10.61186/ijrr.23.1.201","url":null,"abstract":"Background: High-intensity focused ultrasound (HIFU) represents a therapeutic medical procedure that operates by inducing ablation and mechanical disruption.Despite its established efficacy, its potential impact on tumor chemotherapy remains uncertain.Long noncoding RNA (lncRNA) MAFA-AS1 facilitated cancer cell proliferation and fostering drug resistance, but the precise significance and functional implications of MAFA-AS1 in the context of ovarian cancer (OC) remain largely unexplored.The objective of this experiment is to further investigate the potential of HIFU in inhibiting the chemotherapy resistance mechanism of OC. Materials and Methods: Five types of human ovarian cancer cells were employed in this study.There were four different groups, namely MAFA-AS1 siRNA, si-NC, pcDNA3.1-HIF-1(hypoxiainducible factor-1), and pcDNA3.1-control.Quantitative real-time PCR, cell proliferation assay, apoptosis assay, western blot assay, subcellular fractionation, aerobic glycolysis analysis, RNA immunoprecipitation (RIP), and luciferase reporter assay were conducted for experimentation and validation.Results: Significant upregulation of MAFA-AS1 in OC cells was observed.Through loss-of-function experiments based on HIFU, we unraveled its oncogenic functions in OC.MAFA-AS1 was discovered to bind to HIF-1 mRNA, thereby enhancing its expressed stability.Further investigations revealed an interaction between MAFA-AS1 and fat mass and obesity-associated protein (FTO), positively modulating HIF-1 mRNA stability in an FTO -dependent manner.Importantly, MAFA-AS1 exerts its effects on OC by acting through HIF-1.Conclusion: The study underscores the role of MAFA-AS1 in promoting aerobic glycolysis and chemical resistance in OC by up-regulating HIF-1 expression, suggesting that targeting MAFA-AS1 holds promise as a therapeutic strategy for OC patients undergoing chemotherapy.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"23 1","pages":"201-210"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1312-bafa462.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNA SSC4D as a potential diagnostic biomarker promotes gastric cell proliferation and metastasis","authors":"Jingyi Zhou, Tao Jiang, Qi Qi","doi":"10.61186/ijrr.22.4.963","DOIUrl":"https://doi.org/10.61186/ijrr.22.4.963","url":null,"abstract":"","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"22 4","pages":"963-970"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-delivered miRNA-339 represses the progression of gastric cancer via targeting ZNF689 before chemoradiotherapy","authors":"Linhui Liang, Zhen Zhang, Ziqiang Cheng, H. Li, Huimin Jiang","doi":"10.61186/ijrr.22.2.443","DOIUrl":"https://doi.org/10.61186/ijrr.22.2.443","url":null,"abstract":"","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"22 2","pages":"443-453"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value and clinical relevance of tertiary lymphoid structures in non-muscle-invasive bladder cancer","authors":"JB. Ji, P. Li, TT. Yuan, ZK. Qu, GX. Song, ML. Bao, XD. Liang, Binbin Jiang, X.L. Yin, Yingying Wang, Hongli Ji","doi":"10.61186/ijrr.22.2.277","DOIUrl":"https://doi.org/10.61186/ijrr.22.2.277","url":null,"abstract":"Background:The presence of tertiary lymphoid structures (TLS) is related with good prognosis of various cancers including bladder cancer.The majority of bladder tumors are identified as non-muscle-invasive bladder cancer (NMIBC) when they are discovered in their early stages.We aimed to evaluate the prognostic value and clinical relevance of TLS in NMIBC.Materials and Methods: This cohort included 130 NMIBC samples, with 39 (30%) having TLS, as confirmed by hematoxylin and eosin and immunohistochemistry staining.The chi-square test was utilized to examine the relationship between TLS and the biomarkers and clinicopathologic characteristics of NMIBC.Results: It was found that TLS has significant association with pT stage, Ki-67 expression, and COX-2 expression.Using Kaplan-Meier analysis and Gehan-Breslow-Wilcoxon test, we found that the presence of TLS is associated with longer recurrencefree survival and overall survival of NMIBC patients.Moreover, we used bioinformatics to analyze the association of TLS marker L1CAM and bladder cancer.L1CAM is downregulated in bladder cancer, linked to an advanced stage of the disease, and indicates a poorer prognosis for those who have bladder cancer.In bladder cancer, there is a positive correlation between its expression and B cell infiltration.We also indicated that NMIBC with TLS have a higher level of TLS than that without TLS. Conclusion:To conclude, the presence of TLS is an important favorable prognostic indicator in NMIBC.A further understanding on TLS can provide new insights to improve immunotherapy for bladder cancer.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"22 2","pages":"277-282"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1170-9d625af.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"m6A methylation pattern of SLC7A11 mediates the effects of cinobufagin on hepatocellular carcinoma cell proliferation and radioresistance","authors":"Fei Ma, Wen Zhang, Xuesong Sun","doi":"10.61186/ijrr.22.2.481","DOIUrl":"https://doi.org/10.61186/ijrr.22.2.481","url":null,"abstract":"Background: To determine whether and how cinobufagin regulates hepatocellular carcinoma (HCC) cell proliferation and radioresistance.Materials and Methods: Radiosensitive (HepG2-NC) and radioresistant (HepG2-SR) HCC cells were treated with cinobufagin, X-ray ionizing radiation (IR) or a combination of cinobufagin and IR at different doses.Cell counting was performed using the Cell Counting Kit-8 assay.Key ferroptosis marker levels were determined using the indicated methods.RNA immunoprecipitation using an anti-m6A antibody followed by quantitative polymerase chain reaction was performed to determine the m6A levels in SLC7A11 mRNA.Results: Cinobufagin inhibited the proliferation of HepG2-NC and HepG2-SR cells.Exposure to X-rays decreased the HepG2-NC cell count in a time-and dose-dependent manner, but did not affect HepG2-SR cells.A low dose of cinobufagin did not change the cell count without IR exposure, but re-sensitized HepG2-SR cells to IR.The combination of low-dose cinobufagin and IR increased ferroptosis and decreased SLC7A11 expression levels.Mechanistically, the combination of a low dose of cinobufagin and IR decreased m6A levels in the 3' UTR of SLC7A11 in a METTL3-dependent manner.Conclusion: A low dose of cinobufagin exerted synergistic effects with IR and re-sensitized radioresistant HCC cells to IR via a METTL3/m6A-dependent pathway.","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"22 2","pages":"481-486"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://ijrr.com/files/site1/user_files_fad21f/admin-A-10-1-1197-7bd866c.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation on the therapeutic effect of chemotherapy combined with Banxia Xiexin decoction on duodenal cancer under CT enhanced scanning","authors":"Y. Gou, H. Zhou","doi":"10.61186/ijrr.21.4.633","DOIUrl":"https://doi.org/10.61186/ijrr.21.4.633","url":null,"abstract":"","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"8 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139327285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed tomography guided nerve interventional therapy in ischemic cerebrovascular disease","authors":"Y. Quan, Y. Jin, Y. Liu, S. Hu, X. Yin, M. Jin, W. Jiang","doi":"10.61186/ijrr.21.4.707","DOIUrl":"https://doi.org/10.61186/ijrr.21.4.707","url":null,"abstract":"","PeriodicalId":48815,"journal":{"name":"International Journal of Radiation Research","volume":"18 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139329529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}