Бюллетень физиологии и патологии дыхания最新文献

{"title":"The effect of <i>TRPV1</i> gene polymorphism on the development of progressive course of chronic obstructive pulmonary disease","authors":"D. A. Gassan, D. E. Naumov, O. O. Kotova, I. Yu. Sugaylo, E. G. Sheludko, E. Yu. Afanas’eva, Y. G. Gorchakova","doi":"10.36604/1998-5029-2023-89-36-43","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-36-43","url":null,"abstract":"Introduction. It is known that the course of chronic obstructive pulmonary disease (COPD) may vary depending on the rate of bronchial obstruction progression. It has been shown that TRPV1 channels play an important role in the formation of some pathological syndromes typical for COPD. Aim . To study the effect of single nucleotide polymorphisms (SNPs) of TRPV1 gene on the rate of bronchial obstruction progression in COPD patients. Materials and methods . We examined 103 patients with COPD, including 47 with rapid progression of bronchial obstruction (decrease in FEV1≥50 ml/year). SNPs rs460716, rs222749, rs222747, and rs8065080 were genotyped by LATE-PCR. Results . We established that rs460716 SNP had a significant effect on the development of progressive bronchial obstruction in COPD. The CC+TT genotypes were more common among patients with progressive disease (48.9% versus 23.2%), while the heterozygous genotype was protective (p=0.006). Differences remained significant after adjusting for sex, age, pack-year index and baseline FEV 1 (OR 3.2; 95%CI [1.32; 7.57], p=0.009). Conclusion . The obtained results indicate a negative influence of the homozygous genotypes carriage for TRPV1 rs460716 SNP on the course of COPD.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bronchopulmonary dysplasia in children of the Amur region: the structure of the disease","authors":"S. V. Suprun, N. O. Abdulina, S. V. Pichugina, T. A. Sorvina, G. P. Evseeva, O. A. Lebedko","doi":"10.36604/1998-5029-2023-89-95-101","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-95-101","url":null,"abstract":"Aim. Analysis of the structure of bronchopulmonary dysplasia (BPD) in children of the Amur region at the stage of medical care in outpatient and inpatient conditions of the Research Institute of MCP for the period from 20102021. Materials and methods. The study used a retrospective analysis of outpatient records and medical histories of 146 patients with BPD aged from 1 month to 13 years. Results. Among children with BPD, boys accounted for 56.6%, girls 44.3%. Preterm infants accounted for 84.9%, full-term babies – 15.1%. The classic form of the disease was diagnosed in 56% of children, the new form – in 44%. Since 2013, there has been a decrease in the frequency of occurrence of the classical form (in 2011 – 100%, in 2021 – 0%). The opposite pattern is typical for the new form (in 2010 – 14.3%, in 2021 − 100%). Since 2018, there has been a tendency for the new form to prevail over the classical form. Concomitant pathology of the respiratory system was diagnosed in 40.2% of patients (5.03% of them were malformations, stridor – 33.3%, their combination – 1.9%), and pathology of the cardiovascular system in 3.8% of cases. Conclusion. The assessment and dynamics of the structure of BPD disease in the Amur region at the present stage is presented. We report the predominance of a new form of BPD over the classical one. The study noted the frequency of concomitant pathology on the part of the respiratory and circulatory systems in children with BPD. The identified features will be used to search for risk factors for the development and prognosis of this pathology.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intergenic interactions of <i>ADRB2</i>, <i>ADRB3</i>, <i>FABP2</i> and <i>PPARG</i> genes in children with asthma associated with obesity","authors":"G. P. Evseeva, R. S. Telepneva, E. B. Nagovitsyna, S. V. Suprun, O. A. Lebed’ko","doi":"10.36604/1998-5029-2023-89-69-78","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-69-78","url":null,"abstract":"Introduction. The constant increase in the prevalence of bronchial asthma in children and adolescents raises concerns about a parallel increase in obesity-related asthma and suggests that obesity alters asthma towards a phenotype that is more difficult to control. The development of multifactorial diseases (asthma and obesity) is based on complex intergenic interactions that must be taken into account when predicting the risk of developing an unfavorable course of the pathological process. Aim. To study the contribution of polymorphic variants of the metabolism genes rs1042713 (Arg16Gly) and rs1042714 (Gln27Glu) of the β 2 -adrenoceptor gene ( ADRB2 ), rs4994 Trp64Arg of the β 3 -adrenoceptor gene ( ADRB3 ), rs1801282 of the PPARG gene, rs1799883 Ala54Thr of the FABP2 gene using Multifactor Dimensionality Reduction (MDR) in patients with asthma and obesity to identify significant intergenic interactions. Materials and methods. 161 children withasthma were examined, including 59 patients with obesity of 1-3 degrees. The examination included general clinical, laboratory, and functional methods. The level of asthma control was determined according to the GINA criteria (2018). The study of gene polymorphisms was carried out by real-time polymerase chain reaction using sets of “Metabolism” (Research and Production Company “Litekh”, Moscow) on the CFX-96 Biorat device (USA). Results. When comparing groups of children with asthma associated with obesity, a significant model of the interaction of the ADRB3 and FABP2 genes was determined. This two-locus model of intergenic interaction. According to this model, children with bronchial asthma with the following genotypes have an increased risk of obesity: Trp64Arg ADR Β 3 and Ala54Thr FABP2 ; Trp64Arg ADR Β 3 and Thr 54Thr FABP2 ; Trp64 Trp ADR Β 3 and Ala54Thr FABP2 ; Trp64 Trp ADR Β 3 and Thr 54Thr FABP2 . In patients with AD associated with obesity and lack of disease control, we identified another statistically significant two-locus model of the interaction of the rs1042713 genes of the ADRB2 gene and PPARG . According to this model, children with the following genotypes have an increased risk of lack of disease control in patients with obesity-associated asthma: Arg16Gly ADRB2 and Pro12Ala PPARG ; Arg16Gly ADRB2 and Ala12Ala PPARG ; Gly16Gly ADRB2 and Pro12Ala PPARG ; Gly16Gly ADRB2 and Ala12Ala PPARG . Conclusion. The obtained results of the analysis of intergenic interactions indicate that a key role in the formation of predisposition to obesity in asthma patients belongs to the association of polymorphic variants of the ADRB3 (rs4994) and FABP2 (rs1799883) genes, and the risk of uncontrolled asthma in obese children are patients with a combination of polymorphisms rs1042713 of the ADRB2 gene and rs1801282 of the PPARG gene, as evidenced by revealed two-locus models of intergenic interactions that determine the predisposition to obesity in children with asthma and affect the course of the disease. Id","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outpatient management of sarcoidosis in the COVID-19 pandemic","authors":"I. V. Demko, M. G. Mamaeva, N. V. Gordeeva, M. N. Chashchina, A. A. Novikova, N. Yu. Pavlova","doi":"10.36604/1998-5029-2023-89-51-58","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-51-58","url":null,"abstract":"Introduction. COVID-19 infection alters the body’s immune tolerance, which can affect the course of systemic diseases caused by alterations in immune function. Patients with sarcoidosis, just like patients with systemic diseases, have impaired immune system function. They receive immunosuppressive therapy, so they are at risk of infectious diseases, including the viral ones. Aim. To investigate the course of sarcoidosis in the period 2020-2022, the incidence of COVID-19 infection in patients treated by the pulmonologist of the sarcoidosis-polyclinic office of the Krasnoyarsk Regional Clinical Hospital and to evaluate the incidence of sarcoidosis after COVID-19. Materials and methods. 301 outpatient charts of patients diagnosed with sarcoidosis were analyzed. Anamnestic data, information on vaccination against COVID-19, results of physical examination, multispiral computed tomography of thoracic organs in dynamics, data and methods of morphological verification, the volume of the received therapy for sarcoidosis were assessed. Results. There was evidence that the combination of active sarcoidosis and COVID-19 did not contribute to a severe course of viral infection and progression in the course of granulomatosis. In patients with baseline moderate or severe pulmonary function impairment due to sarcoidosis and long-term use of systemic glucocorticosteroids, no increase in the incidence of adverse COVID-19 outcomes was noted. When analyzing fatal cases, it became known that in the first case, chronic recurrent sarcoidosis and prolonged use of systemic glucocorticosteroids were the factors determining the adverse prognosis in COVID- 19 In the second case, the combination of sarcoidosis with cardiovascular disease, diabetes mellitus, and obesity were adverse outcome factors. New cases of sarcoidosis after COVID-19 have been reported, this may be explained by the interaction between SARS-CoV-2 and human immune system, imbalance of proand anti-inflammatory cytokines, disruption of interferon production. However, this statement requires further research.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostics of disturbances in the functional state of the diaphragm in patients with chronic obstructive pulmonary disease after COVID-19","authors":"I. G. Menshikova, E. V. Magalyas, I. V. Sklyar","doi":"10.36604/1998-5029-2023-89-44-50","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-44-50","url":null,"abstract":"Aim. Assessment of the functional state of the diaphragm in patients with COPD who underwent COVID-19. Materials and methods. The study included 35 patients with COPD complicated by compensated chronic cor pulmonale (CCP): group 1 included 15 patients with COPD who had COVID-19, group 2 included 20 patients with COPD. Patients were examined 3 months after suffering COVID-19. The functional state of the diaphragm was studied using the VIVID S70N ultrasound diagnostic system. Results. The greatest decrease in the diaphragm dome height was detected in the 1st group (p=0.001). The excursion of the diaphragm during quiet breathing was increased in both groups, in the 2nd group the indicator was 11% more than in the 1st group. The diaphragm excursion during forced breathing was reduced in the 1st group (p=0.02). The rate of diaphragm excursion during inhalation and exhalation during quiet and forced breathing in both groups was significantly increased compared to the control group. However, in the 1st group, the rate of diaphragm excursion on expiration was 49% higher. These changes may indicate respiratory muscle fatigue in COPD patients who have had COVID-19. The thickness of the muscular part of the diaphragm during inhalation and exhalation during quiet breathing in both groups compared to the control group was significantly higher. These indicators in both groups did not significantly differ from each other. There was a decrease in the thickening fraction of the muscular part of the diaphragm during calm (p=0.01) and forced breathing (p=0.001) in both groups, which indicates the onset of fatigue of the muscles of the diaphragm in the 2nd group and the initial development of weakness of the diaphragm in the 1st group who had the greatest decrease in this indicator during forced breathing. Conclusion. Ultrasonic diagnostic methods significantly expand the possibilities for assessing the functional state of the diaphragm. Patients with COPD who have undergone COVID-19 are characterized by a significant increase in the thickness of the diaphragm, limitation of its mobility, and a slowdown in the rate of relaxation of the muscular part of the diaphragm.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognosis of the risk progression of post-inflammatory pneumofibrosis in children with chronic nonspecific lung diseases","authors":"E. V. Knizhnikova, G. P. Evseeva, S. V. Pichuginа, E. B. Nagovitsyna, S. V. Suprun, O. A. Lebed’ko","doi":"10.36604/1998-5029-2023-89-88-94","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-88-94","url":null,"abstract":"Introduction. Pneumofibrosis (PF) is a morphological outcome of acute and chronic lung diseases, the progression of which leads to organ deficiency. Aim. Development of a prognostic algorithm for assessing the risk of progression of post-inflammatory PF in children with chronic nonspecific lung diseases (CNSLD). Materials and methods. The examination of 52 children with CNSLD with focal post-inflammatory PF was conducted, of which 26 children with progressive PF and 26 children with non-progressive PF. The children were selected according to the “case-control” scheme. The patients had a dynamic clinical and laboratory examination with multispiral computed tomography of the lungs. Determination of the “zero” genotypes of the detoxification genes GSTM1 , GSTT1 was carried out by means of polymerase chain reaction. The odds ratio indicator was used to assess the relative risk. Results. Based on the analysis of medico-social, clinical characteristics and genetic polymorphism, an individual prognostic algorithm for the risk of progression of post-inflammatory PF in children with CNSLD was developed. The algorithm includes the calculation of the total score (TS) of 7 medical and social indicators (living in an urban area; the presence of passive smoking; the duration of a pulmonological history of 4-9 years; the presence of congenital lung malformation; the number of acute respiratory infections (ARI) 4 or more times/year; the duration of ARI 11 days or more; taking antibiotics for more than 3 once/year) and the presence of deletions in the detoxification genes GSTM1 and GSTT1 . With a value of TS 6.97 or more, a high risk of progression of PF is predicted, with TS 3.47-6.96 points, a moderate risk of progression of PF is diagnosed, with TS 3.46 or less points, a minimal risk of progression of PF. Conclusion. The proposed algorithm allows predicting the risk of progression of post-inflammatory PF in children with CNSLD at the stage of early diagnosis and will prevent the spread of the process in the lungs with the help of preventive measures and preventive therapy, which will increase the duration and improve the quality of life of the patient.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent effect of ethanolamine of eicosapentaenoic acid on the synthesis of oxylipins by blood cells of patients with bronchial asthma &lt;i&gt;in vitro&lt;/i&gt;","authors":"I. S. Kovalenko, N. V. Bocharova, T. P. Novgorodtseva, Yu. K. Denisenko","doi":"10.36604/1998-5029-2023-89-18-24","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-18-24","url":null,"abstract":"Introduction. The search for new highly effective methods for the treatment and control of bronchial asthma is an urgent task in pathophysiology and pharmacology. A promising substance for the regulation of systemic chronic inflammation is N-acylethanolamine (EPEA) of eicosapentaenoic acid, which exhibits immunoregulatory properties. Aim. To study the dose-dependent effect of eicosapentaenoic acid ethanolamine on the synthesis and metabolism of oxylipins by blood cells in patients with asthma under in vitro conditions. Materials and methods. The object of the study was the whole blood of 5 patients with controlled mild-to-moderate asthma and 6 healthy people. The in vitro experiment was carried out in lipopolysaccharide-stimulated blood after incubation for 30 minutes. Then the experimental substance N-acyl-ethanolamine of eicosapentaenoic acid (NAE 20:5) was added at concentrations of 1.0, 5.0 and 10.0 µM and incubated at 37ºC for 6 hours in gentle mixing mode. The level of oxylipins was studied by enzyme immunoassay. Results. Experimental exposure to N-acylethanolamines of eicosapentenoic acid had the following effects. Under the influence of ethanolamine of eicosapentaenoic acid at a concentration of 1 µM, the level of PGE2 decreased by 51% (p<0.001) and the amount of 15-HEPE increased by 32% (p<0.05) in lipopolysaccharide-induced blood plasma relative to the values before exposure to ethanolamine. Also EPEA at this dosage showed a tendency to increase the level of 18HEPE. Exposure to EPEA at 5µM resulted in a significant decrease in LTB4 levels by 34% (p<0.001), PGE2 levels by 51% (p<0.001), as well as an increase in 12-HEPE levels by 33% (p<0.01), 15-HEPE by 36% (p<0.05) and 18-HEPE by 87% (p<0.01). Under the influence of EPEA at a dosage of 10 µM, a statistically significant effect on the entire spectrum of the studied oxylipins was revealed. Thus, the use of this dose of ethanolamide in LPS-induced blood showed a decrease in the concentration of LTB4 by 37% (p<0.001), LXA4 by 22% (p<0.05), PGE2 by 50% (p<0.001) and an increase in 5HEPE concentration by 25% (p<0.05), 12-HEPE by 76% (p<0.001), 15-HEPE by 75% (p<0.001), 18-HEPE by 155% (p<0.001) relative to pre-EPEA values. Conclusion. Further study of NAE fatty acids opens up new perspectives in the study of targeted methods for correcting the inflammatory response in bronchial asthma.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phagocyte population in the inflammatory pattern of the bronchi of asthma patients regulated by interleukin-17A and interferon-gamma during the airway response to a cold stimulus","authors":"A. B. Pirogov, A. G. Prikhodko, D. E. Naumov, J. M. Perelman","doi":"10.36604/1998-5029-2023-89-8-17","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-8-17","url":null,"abstract":"Introduction. Based on the common effector functions of polymorphonuclear neutrophils and macrophages as phagocytic cells, their role in the formation of an acute reaction of the respiratory tract to a cold stimulus in patients with asthma is of concern. Aim. The study of the concentration of phagocytes, IL-17A and IFN-γ in the inflammatory pattern of the bronchi of asthma patients depending on the airway reaction to a cold stimulus. Materials and methods. 129 patients with asthma were examined. The design of the study included questioning patients using a validated questionnaire Asthma Control Test (ACT, Quality Metric Inc., 2002), collection of induced and spontaneously produced sputum, exhaled breath condensate (EBC), bronchoprovocation test with a 3-minute isocapnic hyperventilation with cold (-20ºС) air (IHCA) with an assessment of the airway response (ΔFEV 1 ) by spirometry. Results. Group 1 (n=55) included individuals with ΔFEV 1 -10% and below, group 2 (n=74) – with ΔFEV 1 above -10%: -15 (-21; -11) and -3.7 (-6.1; -0.38)%, respectively (p=0.0002). According to the level of ACT (17 [13; 21.5] and 19 [14; 22] points) and indicators of lung function (FEV1 [93.0±2.4 and 97.1±2.4%] and FEF25-75 [63.5±3.5 and 72.0±3.7%]), the patients had no significant intergroup differences. The pattern of bronchial inflammation in group 1 was mixed (neutrophils ≥40%), in group 2 – eosinophilic. In response to the IHCA test, the number of neutrophils significantly increased in the sputum of patients in group 1, the number of macrophages and the number of structurally intact epithelial cells decreased, in proportion to this, the level of IFN-γ and IFN-γ-inducible protein IP-10 (CXCL10) increased in the EBC in relation to patients of the 2nd group. A direct relationship was found between baseline concentrations of IP-10 and IFN-γ (Rs=0.7; p<0.01) in EBC. Conclusion. The airway response to a cold stimulus of patients with asthma is accompanied by functional activation of phagocytic cells with an escalation of neutrophilic inflammation and a decrease in the number of macrophages infiltrating the bronchi associated with an increase in the concentration of IFN-γ, which stimulates the processes of respiratory burst and triggers cell destruction and cytolysis.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of a rare pathogen &lt;i&gt;Chryseobacterium indologenes&lt;/i&gt; in patients with pneumonia","authors":"A. O. Golubeva, A. P. Bondarenko, Yu. A. Natykan, O. E. Trotsenko, O. P. Kurganova, N. Yu. Pshenichnaya, E. K. Taran","doi":"10.36604/1998-5029-2023-89-59-68","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-59-68","url":null,"abstract":"Introduction. Chryseobacterium indologenes bacteria are rarely detected, poorly studied opportunistic bacteria of the nonfermenting gram-negative bacilli group that are characterized by high intrinsic and acquired multidrug resistance and involvement in healthcare associated infections. Aim. To analyze detection rate of C. indologenes strains from various biomaterials obtained from patients with pneumonia, as well as levels of pathogen drug resistance to antimicrobial drugs. Materials and methods. The research included 1740 clinical samples obtained from patients with pneumonia hospitalized in in-patient facilities of the Khabarovsk city during the COVID-19 pandemic. Clinical samples included 1305 respiratory samples (744 sputum samples and 561 nasopharyngeal swabs), 435 samples of autopsy material (lung tissue) obtained from patients with a fatal outcome of the disease. The study was performed via classical bacteriological method. Pathogens were identified with Vitek 2 bacteriological analyzer and Vitek MS mass spectrometer. Sensitivity to antimicrobial drugs was determined by disk-diffusion method and in a bacteriological analyzer. Results. A comparative analysis of detection of the entire nonfermenting gram-negative bacteria (NFGNB) group and C. indologenes from various clinical samples was carried out. Detection rate of NFGNB equaled 17.3%. NFGNB were isolated from autopsy material most often ‒ in 31.7% of cases, less often ‒ from sputum and nasopharyngeal smears (13.8% and 10.7%, respectively). C. indologenes was detected in 0.5% of the cases (8 isolates out of 1740 samples). Isolation of this pathogen was most frequent in nasopharyngeal smears – in 1.1% (6 cases out of 561 samples), least frequent ‒ from sputum, which totaled 0.3% (2 isolates from 744 samples). The detection rate of the pathogen in respiratory samples equaled 0.6% (8 isolates out of 1305 samples). This pathogen was not found in the autopsy material. Carbapenem-resistant variants of C. indologenes were isolated in 75% (6 cases out of 8) of the cases. Conclusion. Further intensifying of diagnostic methods will result in more frequent detection of these unusual pathogens, clinical significance of which needs more justification.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}