Бюллетень физиологии и патологии дыхания最新文献

{"title":"Changes in the blood flow in the uterine and umbilical arteries during the second trimester of pregnancy in exacerbation of bronchial asthma associated with reactivation of cytomegalovirus infection","authors":"L. G. Nakhamchen, I. N. Gorikov, A. N. Odireev, A. A. Sinyuk, T. V. Smirnova","doi":"10.36604/1998-5029-2023-89-123-128","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-123-128","url":null,"abstract":"Aim. To evaluate changes in blood flow in the uterine and umbilical arteries in the second trimester of gestation in women with exacerbation of bronchial asthma associated with reactivation of cytomegalovirus infection (CMVI). Materials and methods. Using Doppler analysis, 115 patients were examined at 21-24 weeks of pregnancy, uncomplicated and complicated by exacerbation of mild to moderate asthma of cytomegalovirus etiology. The first group was represented by 30 seronegative women with uncomplicated pregnancy. The second group included 30 patients with mild asthma in the stage of exacerbation associated with CMVI, leading to chronic compensated placental insufficiency. The third group consisted of 30 patients with exacerbation of moderate asthma caused by CMVI reactivation, which initiated the development of chronic compensated placental insufficiency. The fourth group consisted of 25 women with moderate asthma in the acute stage against the background of the acute phase of CMVI, leading to the formation of chronic subcompensated placental insufficiency. Results. In women in the second group, in comparison with the first group, the following changes were determined: in the right uterine artery, the systolic-diastolic ratio (SDR) was, respectively, 2.75±0.07 and 2.04±0.03 rel. units (р<0.001), pulsation index (PI) – 1.15±0.04 and 0.76±0.02 rel. units (p<0.001), resistance index (RI) – 0.63±0.01 and 0.51±0.01 rel. units (p<0.001); in the left uterine artery, SDR was, respectively, 2.84±0.09 and 1.98±0.05 rel. units (p<0.001), PI – 1.20±0.05 and 0.74±0.03 rel. units (p<0.001), RI – 0.64±0.01 and 0.49±0.01 rel. units (p<0.001); in the absence of statistically significant differences in the indicators of SDR in the umbilical artery – 3.62±0.09 and 3.41±0.06 rel. units, respectively (р>0.05), PI – 1.23±0.03 and 1.19±0.03 rel. units (p>0.05) and RI – 0.73±0.01 and 0.70±0.01 rel. units (p>0.05). In the third group, in comparison with the second one, vascular resistance did not differ significantly in the right, left uterine arteries and the umbilical cord artery. In patients of the fourth group, in contrast to the third one, higher values of resistance to blood flow were observed: in the right uterine artery – SDR (3.41±0.07 and 2.87±0.07 rel. units, p<0.001), PI (1.48±0.07 and 1.18±0.03 rel. units, p<0.001), RI (0.70±0.01 and 0.65±0.01 relative units, p<0.001); in the left uterine artery – SDR (3.33±0.11 and 2.88±0.09 rel. units, p<0.01), PI (1.45±0.05 and 1.19±0.05 rel. units, p<0.001), RI (0.70±0.01 and 0.68±0.01 rel. units, p<0.01); and also in the umbilical artery – SDR (4.39±0.13 and 3.65±0.12 rel. units, p<0.001), PI (1.45±0.04 and 1.24±0.03 rel. units, p<0.001), RI (0.79±0.01 and 0.72±0.01 rel. units, p<0.001). Conclusion. In the pathogenesis of reduced blood flow in the basin of the uterine and umbilical arteries in women with exacerbation of moderately severe asthma of cytomegalovirus etiology, leading to the development of chronic subcompen","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in immunity during the second trimester of pregnancy in women with exacerbation of bronchial asthma associated with reactivation of cytomegalovirus infection","authors":"A. N. Odireev, I. N. Gorikov, L. G. Nakhamchen, I. A. Andrievskaya, N. A. Ishutina, A. V. Prokopenko","doi":"10.36604/1998-5029-2023-89-117-122","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-117-122","url":null,"abstract":"Aim. To evaluate changes in immunity in the second trimester of pregnancy in women with exacerbation of bronchial asthma associated with reactivation of cytomegalovirus infection (CMVI). Materials and methods. The concentration of secretory immunoglobulin A (sIgA), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM) and circulating immune complexes (CIC) was studied in 112 women at 21-24 weeks of uncomplicated pregnancy and pregnancy complicated by exacerbation of mild-to-moderate asthma associated with CMVI. The first group included 30 women seronegative for CMV, with an uncomplicated pregnancy. The second group consisted of 30 patients with exacerbation of mild asthma, initiated by the acute phase of CMVI, which induces the development of chronic compensated placental insufficiency. The third group consisted of 27 patients with moderate asthma in the acute stage against the background of the acute phase of CMVI, leading to the formation of chronic compensated placental insufficiency. The fourth group included 25 patients with exacerbation of moderate asthma with CMV etiology, initiating the development of chronic subcompensated placental insufficiency. Results. In women of the second group, the concentration of sIgA was 5.91±0.371 mg/L, IgA – 2.64±0.089 mg/mL, IgG – 15.4±0.791 mg/mL, IgM – 2.32±0.144 mg/mL, CIC – 0.176±0.004 optical density units (in the first group, respectively, 4.31±0.266 mg/L, p<0.001; 2.37±0.06 mg/mL, p<0.05; 13.3±0.293 mg/mL, p<0,05; 1.11±0.06 mg/mL, p<0.001; 0.092±0.005 optical density units, p<0.001). In patients of the third group, in contrast to the second one, no differences in immunity parameters were detected. In the fourth group, in comparison with the third one, lower parameters of sIgA (3.21±0.213 mg/L, p<0.001), IgA (1.43±0.081 mg/mL, p<0.001), as well as higher IgG values were detected (19.8±0.418 mg/mL, p<0.001), IgM (2.94±0.082 mg/mL, p<0.01) and CIC (0.198±0.005 optical density units, p<0.05). Conclusion. In patients with moderate asthma with exacerbation against the background of CMVI reactivation, which initiates the formation of chronic subcompensated placental insufficiency, in contrast to women with moderate asthma in the stage of CMVI exacerbation in the second trimester of pregnancy, leading to chronic compensated placental insufficiency in the third trimester of gestation, a more pronounced imbalance of humoral immunity and autoimmune disorders are found, which are important in the development of chronic placental dysfunction.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"223 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition of phospholipids of erythrocyte membranes in newborns from mothers with virus pneumonia caused by SARS-CoV-2 in the third trimester of pregnancy","authors":"N. A. Ishutina, I. A. Аndrievskaya, I. V. Dovzhikova, N. N. Dorofienko","doi":"10.36604/1998-5029-2023-89-129-134","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-129-134","url":null,"abstract":"Aim. To study the composition of phospholipids in erythrocyte membranes of newborns from mothers who had viral pneumonia caused by SARS-CoV-2 in the third trimester of pregnancy. Materials and methods. The study included newborns who were born to mothers diagnosed with COVID-19 and community-acquired pneumonia of viral etiology in the third trimester (main group). Of the total number of newborns (n=67), groups of children were formed from mothers with moderate pneumonia (group 1, n=34), and with severe pneumonia (group 2, n=33). The control group consisted of 35 newborns from practically healthy mothers. The quantitative composition of phospholipids in the membranes of erythrocytes of venous blood of the umbilical cord of newborns was determined by the method of two-dimensional thin-layer chromatography according to Kirchner. Results. The study found a statistically significant decrease in the concentration of phosphatidylethanolamine and phosphatidylcholine in erythrocyte membranes in group 1 by 19 and 20%, respectively (p<0.001), in group 2 – by 31 and 29%, respectively (p<0.001); a significant increase in the concentration of lysophosphatidylcholine, sphingomyelin, phosphatidylserine and phosphatidylinositol in group 1 by 43, 8, 15 and 26%, respectively (p<0.001), in group 2 – by 67, 14, 23 and 35%, respectively (p<0.001), compared with their concentration in newborns of the control group. Conclusion. Infection of the mother with SARS-CoV-2 during pregnancy is accompanied by structural disintegration of the erythrocyte membranes of their newborns, manifested by a change in the phospholipid profile in favor of an increase in the fractions of sphingo-, lyso-, inositoland serine phospholipids, which, ultimately, can disrupt the work of the oxygen transport system of the blood, contributing to the development of hypoxia.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the cytokine status in women with exacerbation of bronchial asthma due to reactivation of cytomegalovirus infection in the second trimester of pregnancy","authors":"I. A. Andrievskaya, L. G. Nakhamchen, I. N. Gorikov, N. A. Ishutina, A. N. Odireev, A. V. Prokopenko","doi":"10.36604/1998-5029-2023-89-111-116","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-111-116","url":null,"abstract":"Aim. To study changes in the cytokine status in women with exacerbation of bronchial asthma caused by reactivation of cytomegalovirus infection (CMVI) in the second trimester of gestation. Materials and methods. The concentration of pro-inflammatory cytokines (TNFα, IL-1β, IFNγ, IL-2) in blood serum was assessed in 112 women in the second trimester of pregnancy, uncomplicated and complicated by exacerbation of asthma caused by reactivation of CMVI. The first group included 30 women seronegative for CMVI with uncomplicated pregnancy. The second group consisted of 30 patients with exacerbation of mild asthma associated with CMVI, initiating the formation of chronic compensated placental insufficiency. The third group consisted of 27 patients with exacerbation of moderate asthma induced by CMVI reactivation leading to the development of chronic compensated placental insufficiency. The fourth group included 25 women with exacerbation of moderate asthma due to the acute phase of CMVI, which induces the development of chronic subcompensated placental insufficiency. Results. It was found that in the blood serum of women of the first group, the concentration of TNFα was (Me) 21.5 (13.8–30.1) pg/mL, IL-1β – 18.2 (13.6–34.0) pg/mL, IFNγ – 137.4 (109.5‒174.2) pg/mL and IL-2 – 29.8 (21.0‒38.9) pg/mL. In patients of the second group, compared with the first one, there was an increase in the level of TNFα by 3.79 times (p=0.000001), IL-1β – by 4.8 times (p=0.000001), IFNγ – by 1.73 times (p=0.000001) and IL-2 by 2.91 times (p=0.000001). In the third group, unlike the second one, no significant differences were found between the concentrations of TNFα, IL-1β, IFNγ, and IL-2. In patients of the fourth group, in comparison with the third group, higher values of TNFα (1.35 times, p=0.00507), IL-1β (1.86 times, p=0.000001), IFNγ (1.31 times, p=0.000167), and IL-2 (1.5 times, p=0.0056) were registered. Conclusion. During exacerbation of moderate asthma of cytomegalovirus etiology, leading to chronic subcompensated placental insufficiency, in comparison with exacerbation of moderate asthma caused by reactivation of CMVI, initiating the formation of chronic compensated placental insufficiency, activation of the systemic inflammatory response is most pronounced, leading to stimulation of the migration of monocytes, lymphocytes and neutrophils to the focus of inflammation at the level of small bronchi; also leading to disruption of intersystem relationships and to hemodynamic dysfunction of the placenta.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation of small cell neuroendocrine cancer of the tracheal bifurcation zone, with metastasis to the bone marrow, liver, spleen","authors":"V. V. Voitsekhovskiy, A. A. Grigorenko, T. V. Esenina, S. N. Roshchin, E. A. Filatova, N. A. Fedorova, T. A. Astasheva, M. I. Solovieva, N. V. Tuboltseva","doi":"10.36604/1998-5029-2023-89-135-145","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-135-145","url":null,"abstract":"Aim. Demonstration of a clinical case of a patient with small cell neuroendocrine cancer of the tracheal bifurcation zone, with metastases to the bone marrow, liver, spleen. Materials and methods. A brief review of the literature on the diagnosis of neuroendocrine tumors of the lungs and bronchi is presented, and a clinical observation of small cell neuroendocrine cancer of the tracheal bifurcation zone with metastases to the bone marrow, liver and spleen from the personal practice of the authors is presented. Results. The clinical picture of this patient was dominated by “hematological” manifestations: severe thrombocytopenia and hemorrhagic syndrome, anemia, due to which differential diagnosis with a systemic blood disease was initially carried out. There was no pain syndrome in the bones characteristic of metastatic lesions. Histological examination of the bronchial tumor biopsy and bone marrow trephine biopsy with immunohistochemical examination (IHC) helped in making the diagnosis. In both cases, IHC revealed tumor cells with the same phenotype. Conclusion. Metastasis of tumors to the bone marrow can proceed without characteristic ossalgia and manifest itself only in hematological syndromes (changes in blood tests). Cytological and histological examination of the bone marrow, with IHC, helps in the diagnosis.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"153 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact methods for registering respiratory rate: opportunities and perspectives","authors":"A. A. Garanin, A. O. Rubanenko, I. D. Shipunov, V. S. Rogova","doi":"10.36604/1998-5029-2023-89-159-173","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-159-173","url":null,"abstract":"Introduction. Respiratory rate is known to be one of the most important indicators reflecting the vital functions of a person. An increase in respiratory rate can be found in many diseases and pathological conditions, for example, in chronic obstructive pulmonary disease, pneumonia, bronchial asthma, myocardial infarction, heart failure, anaemia, etc. Due to the active introduction of telemedicine monitoring into clinical practice, the measurement of the abovementioned indicator is particularly relevant for the purpose of early detection and prevention of complications of chronic non-infectious diseases, as well as dynamic monitoring of the condition of patients in both inpatient and outpatient settings. Aim. To search and update information about existing and promising developments for the control of respiratory rate based on different physical principles. Materials and methods. For this review we used databases PubMed, Scopus, MedLine and eLIBRARY. The following keywords were used for the search: “respiratory rate”, “contact”, “measurement”, “sensor”. Results. Contact methods for measuring respiratory rate include a wide range of sensors based on various physical principles. All types of sensors have their own application, but also they have some drawbacks. In order to achieve maximum accuracy of respiratory rate monitoring, it is necessary to carefully assess the conditions in which the patient is located, selecting the most appropriate technological solutions for them. Probably, complex systems, including several different sensors, are able to overcome many shortcomings. In addition, the development of information analysis methods, machine learning and artificial intelligence technologies can increase the sensitivity and accuracy of methods of measuring respiratory rate, reducing the frequency of bias associated with various artefacts. Conclusion. Thus, technological development opens up wide opportunities for long-term monitoring of vital functions, prevention and timely response to adverse events.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of lipid rafts in the immune system and SARS-CoV-2 cell invasion","authors":"E. M. Ustinov, I. A. Andrievskaya, K. S. Lyazgiyan","doi":"10.36604/1998-5029-2023-89-146-158","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-146-158","url":null,"abstract":"Introduction. Glycosphingolipids are compounds composed of hydrophilic sugar structures and hydrophobic ceramides. These molecules form lipid rafts or microdomains in the cell membrane together with cholesterol, sphingomyelin, glycosylphosphatidylinositol and molecules, which determines their properties. Aim. To systematize data on the structure of lipid rafts, their involvement in the functioning of immunocompetent cells and the development of the immune response, and the mechanisms of SARS-CoV-2 viral invasion. Materials and methods. From these positions, literary sources for 1981-2023 are analyzed. Literature search was carried out in information systems: PubMed and Google Scholar. Results. There are separate works that reflect the role of lipid rafts as mediators of signal transduction in the development of innate and adaptive immune responses. Other studies describe their importance in pathogen-host interaction and avoidance of immune control. Recently, studies have appeared on the effect of lipid microdomains of the cell membrane on viral invasion, including that caused by SARS-CoV-2. Conclusion. This review makes a significant contribution to understanding the role of lipid rafts in the functioning of the immune system and viral invasion, which determines the prospects for further research and the possibility of their use as therapeutic targets in the development of immunomodulatory drugs.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology of placental insufficiency in women in the second trimester of gestation during the epidemic of influenza A(H3N2) and pandemic influenza(H1N1)pdm","authors":"I. N. Gorikov, V. I. Reznik, A. A. Sergievich, L. G. Nakhamchen, A. N. Odireev, A. V. Prokopenko, E. S. Таrasyuk, A. G. Sudakov, O. E. Коlomiets, A. V. Gaidarova","doi":"10.36604/1998-5029-2023-89-102-110","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-102-110","url":null,"abstract":"Aim. To study the etiology of placental insufficiency in women in the second trimester of gestation during the influenza A(H3N2) epidemic and the A(H1N1)pdm pandemic. Materials and methods. In 1865 patients in the second trimester of pregnancy, uncomplicated and complicated by exacerbation of chronic ENT pathology leading to the development of placental insufficiency, using laboratory methods in the blood, nasal epithelium and biological fluids, pathogens of monoand mixed infections (influenza A(H1N1)pdm), A(H3N2), influenza B, parainfluenza type 1-3, adenovirus, respiratory syncytial virus (RS), cytomegalovirus (CMV), herpes simplex virus type 1 and type 2 (HSV-1,2 type), candida infection ( Candida albicans ), chlamydia ( Chlamydia trachomatis ), mycoplasmosis ( Mycoplasma hominis ) and ureaplasmosis ( Ureaplasma urealyticum ) were detected. The first group consisted of 873 women with placental insufficiency diagnosed during the influenza A(H3N2) epidemic, the second group consisted of 890 patients with placental insufficiency diagnosed during circulation of the influenza A(H1N1)pdm in the population. The control group were 42 women without ENT diseases and placental insufficiency caused by monoand mixed viral infections during influenza A(H3N2) and 60 women with similar somatic, infectious and obstetric status, examined during pandemic influenza A(H1N1)pdm. Results. With the development of placental insufficiency in women of the second group, compared with the first one, mono-influenza B and parainfluenza types 1-3 are diagnosed less frequently, as well as a combination of influenza A(H1N1) pdm and influenza B; influenza A(H1N1)pdm and parainfluenza types 1-3; influenza A(H1N1)pdm and RS virus; CMV and Chlamydia trachomatis ; Mycoplasma hominis and Ureaplasma urealyticum ; HSV-1,2 type, Candida albicans and Chlamydia trachomatis ; as well as HSV-1,2 type, Candida albicans and Ureaplasma urealyticum . At the same time, exacerbations of mono-CMV, mono-HSV-1,2 type and Candida albicans are more common, as well as combinations of CMV+HSV-1,2 type; CMV+ Candida albicans ; CMV+ Ureaplasma urealyticum ; and CMV+ Mycoplasma hominis ; CMV+HSV-1,2 type+ Candida albicans . Conclusion. In the etiology of placental insufficiency in acute diseases of the ENT organs in women in the second trimester of gestation against the background of circulation of the A(H1N1)pdm influenza in the population, compared with placental dysfunction in pregnant women with a similar ENT pathology during the influenza A(H3N2) epidemic, there is an increase in the proportion of monocytomegalovirus, herpesvirus and candida infections, as well as mixed cytomegalovirus infection, the reactivation of which may be due to more pronounced virusinduced immunosuppression and hormonal status disorders, leading to local dysbiosis.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136343959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of association of the polymorphism rs1799883 of the &lt;i&gt;FABP2&lt;/i&gt; gene in children with bronchial asthma associated with obesity","authors":"R. S. Telepneva, G. P. Evseeva, E. B. Nagovitsyna, E. N. Suprun, S. V. Suprun, O. A. Lebed’ko","doi":"10.36604/1998-5029-2023-89-79-87","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-79-87","url":null,"abstract":"Introduction. Currently, there is a separate phenotype “bronchial asthma – obesity”, manifested by a more severe course of the disease, low rates of achieving asthma control, resistance to basic therapy. Asthma, like obesity, is recognized as a classic example of multifactorial diseases, which are based on a rather complex gene network. The active search for genetic markers characterizing individual characteristics of human metabolism continues. Of particular interest are the genes involved in the regulation of fat and carbohydrate metabolism. Aim. Analysis of associations of polymorphic loci Ala54Thr (G163A) of the FABP2 gene with bronchial asthma of varying severity and control of asthma in children. Materials and methods. 161 children with bronchial asthma in remission were examined by a continuous sampling method, of which 59 patients with obesity of 1-3 degrees without concomitant endocrine pathology. The examination included general clinical, functional, and instrumental methods. The level of asthma control was determined according to the GINA criteria (2018). The biochemical study was carried out on an automatic analyzer SAPPHIRE 400 (Japan). The study of gene polymorphisms was carried out by real-time polymerase chain reaction using sets of “Metabolism” (Research and Production Company “Litekh”, Moscow) on the CFX-96 Biorat device (USA). Results. We have not identified associations of the presence of polymorphic loci of the FABP2 gene with obesity. It was determined that in children with bronchial asthma, the frequency of carrying the homozygous genotype Thr/Thr and the minor allele Thr increased by 1.5 times compared to the control group (OR 9.043; 95%CI [2,093–39,073], p=0.0011 and OR 2.946; 95%CI [1,698‒5,111], p=0.001, respectively), and in children with bronchial asthma with and without asthma control, the carriage of the homozygous Thr/Thr genotype and the rare A allele increased the risk of uncontrolled bronchial asthma (OR 2.42; 95%CI [1.23‒4.79], p=0.03 and OR 1.75; 95%CI [1,119‒2,736], p=0.01), the frequency of the homozygous Ala/Ala genotype and the frequent Ala allele was detected 1.5 times more often in children with bronchial asthma associated with obesity (OR 2.176; 95%CI [1.001‒4.727], p=0.0008 and OR 2.378; 95%CI [1.495‒3.780], p=0.0002, respectively). Conclusion. Although we have not identified associations of the presence of polymorphic loci of the FABP2 gene with obesity, it has been shown that children with Ala54Thr+Thr54Тhr genotypes have significantly higher glucose levels (4.9±0.06 mmol/L compared with carriers of the Ala54Ala genotype 4.0±0.06 mmol/L, p<0.001), cholesterol (4.8±0.4 mmol/L compared with carriers of the Ala54Ala genotype 3.93±0.1 mmol/L, p<0.05) and low density lipoproteins (2.55±0.09 mmol/L compared with carriers of the Ala54Ala genotype 2.26±0.1 mmol/L, p<0.05). There is an obvious need for further investigation of the effect of gene polymorphism on the indicators of carbohydrate and lipid metabol","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The state of mitochondrial membrane potential in peripheral blood leukocytes of patients with chronic obstructive pulmonary disease","authors":"I. Yu. Sugaylo, D. A. Gassan, D. E. Naumov, O. O. Kotova, Y. G. Gorchakova, E. G. Sheludko","doi":"10.36604/1998-5029-2023-89-25-35","DOIUrl":"https://doi.org/10.36604/1998-5029-2023-89-25-35","url":null,"abstract":"Introduction. Mitochondria provide energy homeostasis of the cell by maintaining an optimal transmembrane electrochemical gradient (ΔΨm), which does not allow excessive formation of reactive oxygen species (ROS). However, under conditions of pathology, the normal functioning of mitochondria is disrupted, which can lead to ATP deficiency and/or increased production of ROS. Aim. The aim of this study was to investigate the ΔΨm parameters and their relationship with the expression of TRP channels in peripheral blood leukocytes of patients with chronic obstructive pulmonary disease (COPD). Materials and methods. The study included 23 patients with COPD of varying severity, 8 smokers without signs of bronchial obstruction and 9 healthy volunteers who had never smoked. All subjects underwent spirometry to assess the lung function. ΔΨm was determined by staining the cells with tetramethylrhodamine ethyl ester (TMRE) and measuring the fluorescent signal by flow cytometry, under basal conditions and pro-inflammatory stimulation with phorbol-12-myristate-13-acetate (PMA). Results. We found that COPD patients were characterized by a significant increase in basal ΔΨm of monocytes (161.8 [153.8; 206.8] vs. 129.3 [75.5; 161.8], p=0.03) and lymphocytes (209,7 [184.7; 257.8] vs. 122.5 [67.9; 164.3], p=0.003) as compared with the control group. Stimulation of cells with PMA led to multidirectional changes in ΔΨm, while its increased level was still preserved in COPD. In monocytes of COPD patients, a decrease in ΔΨm in response to PMA stimulation was prevalent (75%), while in the majority (53.9%) of individuals in the control group ΔΨm, on the contrary, increased (p=0.08). In addition, among COPD patients, an increase in ΔΨm in monocytes was accompanied by an enhanced expression of TRPV4, while in the control group, among individuals with positive dynamics of ΔΨm, TRPV4 expression was, on the contrary, reduced. Conclusion. The increased level of ΔΨm in the mononuclears of COPD patients is consistent with previously detected enhanced ROS production, but does not support the assumption about energy deficit in the cells. The revealed differences in the relationship between TRPV4 expression and ΔΨm dynamics may indicate the presence of pathological features in TRP signaling in COPD patients.","PeriodicalId":487542,"journal":{"name":"Бюллетень физиологии и патологии дыхания","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}