Mycokeys最新文献

{"title":"Distribution patterns of <i>Calonectria</i> (Ascomycota, Sordariomycetes, Hypocreales, Nectriaceae) species complexes related to diseased leaves and soil habitats during leaf blight outbreak season in <i>Eucalyptus</i> plantations in southern China.","authors":"WenXia Wu, ShuaiFei Chen","doi":"10.3897/mycokeys.110.130733","DOIUrl":"https://doi.org/10.3897/mycokeys.110.130733","url":null,"abstract":"<p><p>Calonectria leaf blight caused by <i>Calonectria</i> species is one of the most important diseases associated with <i>Eucalyptus</i> plantations in Asia and South America. This study aimed to clarify the distribution patterns of <i>Calonectria</i> species residing in different species complexes associated with diseased trees and soils during leaf blight outbreak season in <i>Eucalyptus</i> plantations in southern China. In this study, 482 <i>Calonectria</i> isolates obtained from diseased <i>Eucalyptus</i> trees and soils under these trees in eight sampling sites in three provinces were identified by DNA sequence analyses of <i>tef1</i>, <i>tub2</i>, <i>cmdA</i>, and <i>his3</i> gene regions. Six species residing in three species complexes were identified: <i>Calonectriapseudoreteaudii</i> and <i>C.acaciicola</i> in the <i>Calonectriareteaudii</i> species complex; <i>C.hongkongensis</i>, <i>C.aconidialis</i>, and <i>C.chinensis</i> in <i>C.kyotensis</i> species complex; and <i>C.auriculiformis</i> in <i>C.cylindrospora</i> species complex. The habitats of <i>Calonectria</i> in different species complexes differed, <i>C.reteaudii</i> species complex inhabits in both diseased trees and soils, <i>C.kyotensis</i> species complex only in soils. The Calonectria leaf blight in the sampled regions was caused by species in the <i>C.reteaudii</i> species complex but not by the species in the <i>C.kyotensis</i> species complex. These findings suggest that the species in the <i>C.reteaudii</i> species complex should receive more attention in disease management, as they are the primary cause of the disease in the sampled regions.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"110 ","pages":"117-140"},"PeriodicalIF":2.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ophiostomatalean fungi associated with <i>Polygraphus</i> bark beetles in the Qinghai-Tibet Plateau, China.","authors":"Zheng Wang, Caixia Liu, Xiuyue Song, Yingjie Tie, Huimin Wang, Huixiang Liu, Quan Lu","doi":"10.3897/mycokeys.110.135538","DOIUrl":"https://doi.org/10.3897/mycokeys.110.135538","url":null,"abstract":"<p><p>Climate change has exacerbated outbreaks of forest pests worldwide. In recent years, bark beetles have caused significant damage to coniferous forests of the Northern Hemisphere. <i>Polygraphus</i> bark beetles are widely distributed secondary pests. Recently, tree mortality caused by these beetles on the Qinghai-Tibet Plateau has been increasing; however, few studies have focused on their fungal associations. In the present study, we explored the diversity of ophiostomatalean fungi associated with these beetles on the north-eastern and southern Qinghai-Tibet Plateau. We isolated 442 ophiostomatalean strains from adult beetles and their fresh galleries, specifically targeting <i>Polygraphuspoligraphus</i> and <i>Polygraphusrudis</i> infesting <i>Piceacrassifolia</i> and/or <i>Pinusgriffithii</i>. Based on phylogenetic and morphological features, we assigned the 442 strains to 16 species belonging to <i>Grosmannia</i> spp., <i>Leptographium</i> spp. and <i>Ophiostoma</i> spp. Amongst these, <i>Ophiostomamaixiuense</i> and <i>Ophiostomabicolor</i> were the most frequently isolated species, accounting for 20.8% and 18.1% of the total number of ophiostomatalean assemblages, respectively. By comparing their fungal communities, we found that the different patterns of fungal assemblages of bark beetles from the north-eastern and southern Qinghai-Tibet Plateau may be influenced by biogeographic barriers and host tree species. The results of this study enhance our understanding of bark beetle fungal assemblages, especially <i>Polygraphus</i>, on the Qinghai-Tibet Plateau, with implications for forest management under changing climate.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"110 ","pages":"93-115"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular identification for four new wood-inhabiting species of <i>Lyomyces</i> (Basidiomycota) from China.","authors":"Qi Yuan, Yunchao Li, Yunfei Dai, Kunyan Wang, Yixuan Wang, Changlin Zhao","doi":"10.3897/mycokeys.110.133108","DOIUrl":"https://doi.org/10.3897/mycokeys.110.133108","url":null,"abstract":"<p><p>Fungi are one of the most diverse groups of organisms on Earth, in which the wood-inhabiting fungi play an important role in forest ecosystem processes and functions. Four new wood-inhabiting fungi, <i>Lyomyceshengduanensis</i>, <i>L.niveomarginatus</i>, <i>L.wumengshanensis</i> and <i>L.zhaotongensis</i>, are proposed, based on morphological features and molecular evidence. <i>Lyomyceshengduanensis</i> differs in the brittle basidiomata with pruinose hymenial surface, a monomitic hyphal system and ellipsoid basidiospores (3.5-6 × 3-4.5 µm). <i>Lyomycesniveomarginatus</i> is distinguished by the subceraceous basidiomata with crackled hymenial surface, a monomitic hyphal system and ellipsoid basidiospores (4.5-7 × 3-4 µm). <i>Lyomyceswumengshanensis</i> is distinguished by the grandinioid hymenial surface, a monomitic hyphal system and ellipsoid to broad ellipsoid basidiospores (4-6 × 3-5 µm). <i>Lyomyceszhaotongensis</i> is unique in the grandinioid hymenial surface, a monomitic hyphal system and broadly ellipsoid basidiospores measuring as 2.6-3.5 × 2.5-3 µm. Sequences of ITS and nLSU rRNA markers of the studied samples were generated and phylogenetic analyses were performed using the Maximum Likelihood, Maximum Parsimony and Bayesian Inference methods. The phylogram, based on the ITS+nLSU rDNA gene regions, included three genera within the Schizoporaceae viz. <i>Fasciodontia</i>, <i>Lyomyces</i> and <i>Xylodon</i>, in which the four new species were grouped into <i>Lyomyces</i>. The phylogenetic tree inferred from the ITS sequences highlighted that <i>L.hengduanensis</i> group with <i>L.zhaotongensis</i> and then closely grouped with <i>L.crustosus</i>, <i>L.ochraceoalbus</i>, and <i>L.vietnamensis</i>. The new taxon <i>L.niveomarginatus</i> was retrieved as a sister to <i>L.juniperi</i>. The new species <i>L.wumengshanensis</i> was sister to <i>L.macrosporus</i>. The new taxon <i>L.zhaotongensis</i> grouped with <i>L.hengduanensis</i> and then closely grouped with <i>L.crustosus</i>, <i>L.ochraceoalbus</i> and <i>L.vietnamensis</i>.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"110 ","pages":"67-92"},"PeriodicalIF":2.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additions to the genus <i>Kirschsteiniothelia</i> (Dothideomycetes); Three novel species and a new host record, based on morphology and phylogeny.","authors":"Xia Tang, Rajesh Jeewon, Ruvishika S Jayawardena, Deecksha Gomdola, Yong-Zhong Lu, Rong-Ju Xu, Abdulwahed Fahad Alrefaei, Fatimah Alotibi, Kevin D Hyde, Ji-Chuan Kang","doi":"10.3897/mycokeys.110.133450","DOIUrl":"10.3897/mycokeys.110.133450","url":null,"abstract":"<p><p>During a survey of microfungi associated with forest plants, four specimens related to <i>Kirschsteiniothelia</i> were collected from decaying wood in Guizhou, Hainan and Yunnan Provinces, China. <i>Kirschsteiniothelia</i> species have sexual and asexual forms. They are commonly found as saprophytes on decaying wood and have been reported as disease-causing pathogens in humans as well. In this study, we introduce three novel <i>Kirschsteiniothelia</i> species (<i>K.bulbosapicalis</i>, <i>K.dendryphioides</i> and <i>K.longirostrata</i>) and describe a new host record for <i>K.atra</i>, based on morphology and multi-gene phylogenetic analyses of a concatenated ITS, LSU and SSU rDNA sequence data. These taxa produced a dendryphiopsis- or sporidesmium-like asexual morph and detailed descriptions and micromorphological illustrations are provided. Furthermore, we provide a checklist for the accepted <i>Kirschsteiniothelia</i> species, including detailed host information, habitat preferences, molecular data, existing morphological type, country of origin and corresponding references.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"110 ","pages":"35-66"},"PeriodicalIF":2.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-phylogenetic evidence reveals novel species and new records of <i>Nigrograna</i> (Nigrogranaceae) associated with medicinal plants in Southwestern China.","authors":"Hong-Zhi Du, Yu-Hang Lu, Ratchadawan Cheewangkoon, Jian-Kui Liu","doi":"10.3897/mycokeys.110.132628","DOIUrl":"10.3897/mycokeys.110.132628","url":null,"abstract":"<p><p>During a survey of saprobic fungal niches in Southwestern China, eighteen ascomycetous collections of <i>Nigrograna</i> (Nigrogranaceae, Pleosporales, Dothideomycetes) were found on dead branches of medicinal plants. These taxa were characterized and identified based on morphological and culture characteristics, and phylogenetic analyses of a combined the internal transcribed spacer region of rDNA (ITS), nuclear large subunit rDNA (28S, LSU), RNA polymerase second-largest subunit (rpb2), nuclear small subunit rDNA (18S, SSU), and translation elongation factor 1-alpha (<i>tef1-α</i>) sequence dataset also confirmed their placement. As a result, four novel species, namely <i>Nigrogranacamelliae</i>, <i>N.guttulata</i>, <i>N.longiorostiolata</i> and <i>N.neriicola</i> were described. Additionally, four new host records of <i>N.acericola</i>, <i>N.magnoliae</i>, <i>N.oleae</i> and <i>N.thymi</i> were introduced. Furthermore, this study addresses the taxonomic status of <i>N.trachycarpi</i>, proposing its synonymy under <i>N.oleae</i>. Detailed illustrations, descriptions and informative notes for each newly identified taxon and novel host record are provided in this study.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"110 ","pages":"1-33"},"PeriodicalIF":2.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-molecular analysis of two new species <i>Deconica</i> and <i>Entocybe</i> in Agaricales from Mount Tianmu, China.","authors":"Yu-Yu Shen, Zi-Wen Zhang, Wen-Qian Li, Xing-Ning Liu, Fei-Ying Tian, Chun-Mei Pang, Wen-Hong Dai, Yao-Bin Song, Ming Dong","doi":"10.3897/mycokeys.109.131298","DOIUrl":"10.3897/mycokeys.109.131298","url":null,"abstract":"<p><p>Two new species of Agaricales, <i>Deconicaflavum</i> and <i>Entocyberoseoalbus</i>, are described from Mount Tianmu, Zhejiang Province, Eastern China. Two new species are distinct and monophyletic based on morphology and phylogenetic analyses. <i>Deconicaflavum</i> differs from other <i>Deconica</i> species in that the pileus is brow shallow in the center and stipe with yellowish white fibrils, scatted on litter under coniferous and broad-leaved mixed forest at 1162 m. <i>Entocyberoseoalbus</i> is distinguished from other species of <i>Entocybe</i> by nearly blue pileus and pinkish-white stipe, scatted on humus in similar forest conditions at 1025 m. The differences are discussed between the two new taxa and their similar species morphologically, and related species phylogenetically.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"109 ","pages":"319-336"},"PeriodicalIF":2.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new wood-inhabiting fungi of <i>Botryobasidium</i> (Cantharellales, Basidiomycota) from subtropical forests of Southwestern China.","authors":"Lin-Jiang Zhou, Xue-Long Li, Hai-Sheng Yuan","doi":"10.3897/mycokeys.109.133325","DOIUrl":"10.3897/mycokeys.109.133325","url":null,"abstract":"<p><p>The basidiomycete genus <i>Botryobasidium</i> is a resupinate saprotrophic with a global distribution range from coniferous to broad-leaved forest ecosystems. Though numerous species have been reported from Eurasia and North America, few have been described from China. In the current work, phylogenetic analyses of <i>Botryobasidium</i> in China were conducted based on the dataset of the internal transcribed spacer (ITS) regions and the large subunit (LSU) of nuclear ribosomal RNA gene. Maximum likelihood and Bayesian analyses were used to reconstruct the phylogenetic tree, and three new species, namely <i>Botryobasidiumacanthosporum</i>, <i>B.leptocystidiatum</i> and <i>B.subovalibasidium</i>, were described from subtropical forests of Yunnan Province, Southwestern China. <i>Botryobasidiumacanthosporum</i> is characterized by having yellowish white to dark yellow basidiome, clavate to tubular cystidia, and subglobose to globose basidiospores with obtuse spines. <i>Botryobasidiumleptocystidiatum</i> is characterized by having fluffy to arachnoid, greyish white to ivory basidiome, generative hyphae with clamped, tubular cystidia, and subnavicular to navicular basidiospores. While, <i>B.subovalibasidium</i> is characterized by having yellowish to ivory basidiome, subovoid basidia, navicular to suburniform basidiospores, and thick-walled chlamydospores. These three new species are described and illustrated, and the discriminating characters between the new species and their closely related species are discussed. A key to known species of <i>Botryobasidium</i> in China is provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"109 ","pages":"337-354"},"PeriodicalIF":2.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Cytospora</i> (Diaporthales, Cytosporaceae) causing canker disease of <i>Malusdomestica</i> and <i>M.sieversii</i> in Xinjiang, China.","authors":"Guifang Cai, Ying Zhao, Yawei Zhai, Meilin Yan, Rong Ma, Daoyuan Zhang","doi":"10.3897/mycokeys.109.131456","DOIUrl":"https://doi.org/10.3897/mycokeys.109.131456","url":null,"abstract":"<p><p>Apple tree canker is a serious disease caused by species of <i>Cytospora</i>. Xinjiang Uygur Autonomous Region is one of the most important apple-producing areas in China. However, losses due to apple <i>Cytospora</i> canker have seriously damaged the apple industry and affected the economic development of the apple growers in this region. In this study, we used morphological characteristics combined with multilocus phylogenetic analyses of the ITS, <i>act</i>, <i>rpb2</i>, <i>tef1</i> and <i>tub2</i> loci to identify isolates from apple (<i>Malusdomestica</i>) and wild apple (<i>M.sieversii</i>). As a result, <i>C.hippophaopsis</i> <b>sp. nov.</b> from <i>M.sieversii and C.shawanensis</i> <b>sp. nov.</b> from <i>M.domestica</i> were discovered and proposed herein. Pathogenicity tests were further conducted on 13 varieties of apple and wild apple, which confirmed <i>C.hippophaopsis</i> and <i>C.shawanensis</i> as canker pathogens. Meanwhile, <i>C.hippophaopsis</i> is generally more aggressive than <i>C.shawanensis</i> on the tested varieties of apple and wild apple.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"109 ","pages":"305-318"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling species diversity within early-diverging fungi from China I: three new species of <i>Backusella</i> (Backusellaceae, Mucoromycota).","authors":"Heng Zhao, Yong Nie, Bo Huang, Xiao-Yong Liu","doi":"10.3897/mycokeys.109.126029","DOIUrl":"https://doi.org/10.3897/mycokeys.109.126029","url":null,"abstract":"<p><p>The species diversity of early-diverging fungi has long lagged behind that of higher fungi, posing a significant obstacle to our comprehensive understanding of the fungal kingdom. Our ongoing research endeavors aim to address this gap by exploring the species diversity of early-diverging fungi in China. In this study, we describe three novel species within the <i>Backusella</i>, namely <i>B.elliptica</i> <b>sp. nov.</b>, <i>B.fujianensis</i> <b>sp. nov.</b>, and <i>B.variispora</i> <b>sp. nov.</b>, based on phylogenetic and morphological analyses. In the phylogenetic analysis of the ITS (internal transcribed spacer), LSU (large subunit of ribosomal RNA gene), and RPB1 (RNA polymerase II largest subunit gene) regions, the <i>B.elliptica</i> and <i>B.fujianensis</i> cluster closely with <i>B.gigacellularis</i>, <i>B.ovalispora</i>, and <i>B.solicola</i>, and the <i>B.variispora</i> is closely related to <i>B.locustae</i> and <i>B.pernambucensis</i>. Morphologically, <i>B.elliptica</i> is distinguished by elliptical sporangiospores, as well as cylindrical and hemispherical columellae. The <i>B.fujianensis</i> is characterized by elliptical sporangiospores, and various types of columellae such as hemispherical, subglobose, depressed globose and conical. The <i>B.variispora</i> is characterized by subglobose to globose sporangiospores, as well as hemispherical, subglobose to globose columellae. Additionally, the sporangiophores are long and monopodially branched in <i>B.elliptica</i> and <i>B.fujianensis</i>, while short and simple or sympodially branched in <i>B.variispora</i>. Physiologically, the maximum growth temperatures of <i>B.elliptica</i> (32 °C), <i>B.fujianensis</i> (35 °C), and <i>B.variispora</i> were (35 °C) were determined. With the inclusion of these newly described taxa, the total number of <i>Backusella</i> species known from China now stands at 12. Finally, we provide a key to facilitate the morphological identification of <i>Backusella</i> species from Asia.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"109 ","pages":"285-304"},"PeriodicalIF":2.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New <i>Helminthosporium</i> (Massarinaceae, Dothideomycetes) and <i>Nigrospora</i> (Incertae sedis, Sordariomycetes) species associated with walnut (<i>Juglansregia</i> L.) in China.","authors":"Mengting Zou, Fatimah Al-Otibi, Kevin David Hyde, Yong Wang, Xue-Jun Pan","doi":"10.3897/mycokeys.109.133431","DOIUrl":"10.3897/mycokeys.109.133431","url":null,"abstract":"<p><p>Six collections of ascomycetes were obtained from samples collected from dead branches and leaves of <i>Juglansregia</i> in Guizhou and Yunnan provinces, China. By incorporating multigene phylogenetic analysis (ITS, LSU, <i>rpb2</i>, SSU, <i>tef1-α</i>, <i>tub2</i>) supplemented by morphological data, we establish two novel species, namely <i>Helminthosporiumguizhouense</i> and <i>Nigrosporayunnanensis</i>. In morphology, <i>H.guizhouense</i> can be distinguished from <i>H.caespitosum</i> by its narrower conidia (13-16 µm vs. 27.3-35.5 µm), and <i>N.yunnanensis</i> is characterized by black, globose conidia (16.2 × 14.4 µm). The phylogenetic results further substantiated them as novel taxa. The present study contributes to our comprehension of the range of fungi found in <i>Juglansregia</i>, thereby expanding our knowledge of the diversity of fungi within this host.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"109 ","pages":"265-284"},"PeriodicalIF":2.8,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}