Fermentation-Basel最新文献

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Current Applications and Future Trends of Dehydrated Lactic Acid Bacteria for Incorporation in Animal Feed Products 脱水乳酸菌在动物饲料中的应用现状及发展趋势
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-08 DOI: 10.3390/fermentation9080742
A. Moretti, N. Brizuela, B. Bravo-Ferrada, E. E. Tymczyszyn, M. Golowczyc
{"title":"Current Applications and Future Trends of Dehydrated Lactic Acid Bacteria for Incorporation in Animal Feed Products","authors":"A. Moretti, N. Brizuela, B. Bravo-Ferrada, E. E. Tymczyszyn, M. Golowczyc","doi":"10.3390/fermentation9080742","DOIUrl":"https://doi.org/10.3390/fermentation9080742","url":null,"abstract":"Several lactic acid bacteria (LAB) species have been recognized as probiotics and are of considerable interest due to their potential ability to confer health benefits upon consumption. In the animal feed sector, probiotics offer an alternative to the use of antibiotic growth promoters. The preservation and incorporation of probiotics into dry products requires carefully meeting several criteria and overcoming technological challenges to maintain their functionality. Drying is a crucial step in the process, but the probiotic properties of the resulting powder and the final cell viability in the food product are significantly influenced by the type of protective compounds and drying techniques employed. In light of the growing demand for functional animal products, this review focuses on the damages incurred during microorganism dehydration processes for food incorporation, and explores strategies to minimize such damages. It provides an overview of the effects of probiotic products in the animal feed industry, including their incorporation in low-moisture food matrices and key considerations for success. Additionally, it highlights postbiotics as an attractive alternative for live probiotic cells with many technological advantages.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44818317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Human Body as an Ethanol-Producing Bioreactor—The Forensic Impacts 人体作为生产乙醇的生物反应器-法医影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-07 DOI: 10.3390/fermentation9080738
I. Šoša
{"title":"The Human Body as an Ethanol-Producing Bioreactor—The Forensic Impacts","authors":"I. Šoša","doi":"10.3390/fermentation9080738","DOIUrl":"https://doi.org/10.3390/fermentation9080738","url":null,"abstract":"Auto-brewery syndrome (ABS), also called gut fermentation syndrome, is an extremely infrequent but also underrecognized disorder where ethanol is produced endogenously, similar to a typical bioreactor. The reliability of forensic alcohol analysis results is frequently challenged as the ethanol concentration in the breath, blood, and/or urine constitutes important evidence for prosecuting drivers under the influence of the alcohol. This further emphasizes the need to understand ABS, as in legal proceedings it is often presented as grounds for acquittal due to the concept that the findings could have corresponded to endogenously produced ethanol. However, this rare and underdiagnosed medical condition should not be considered as purely a lawyer’s favorite argument. Manifestations of ABS can have a severe impact on a patient’s life and pose social consequences as well. Unfortunately, barely anything has been unearthed, and aspects such as genetic susceptibility, gut-mucus-eating microorganisms, and fecal microbiome transplantation were reviewed for the first time in this context. The framework of this review was not limited to the gut microbiota exclusively; moreover, the overgrowth of microorganisms is linked to the use of antibiotics. Studies have indicated that carbohydrate fermentation occurs in locations other than in intra-intestinal flora. Accordingly, the literature was searched for cases of patients with ABS with yeast infections in their genitourinary or oral systems.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42035840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Condensed Tannins on Nitrogen Distribution and Metabolome after Aerobic Exposure of Sainfoin Silage 浓缩单宁对赛恩芬青贮好氧暴露后氮分布和代谢组的影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-07 DOI: 10.3390/fermentation9080739
Rongzheng Huang, Fan-fan Zhang, Xuzhe Wang, C. Ma
{"title":"Effect of Condensed Tannins on Nitrogen Distribution and Metabolome after Aerobic Exposure of Sainfoin Silage","authors":"Rongzheng Huang, Fan-fan Zhang, Xuzhe Wang, C. Ma","doi":"10.3390/fermentation9080739","DOIUrl":"https://doi.org/10.3390/fermentation9080739","url":null,"abstract":"(1) Background: Previous studies have indicated that proteolysis is inhibited by the condensed tannins (CTs) that are present during sainfoin ensiling. Whether inhibiting this effect of CTs on proteolysis is functional during aerobic exposure is still unclear. (2) Methods: the present study investigated the effect of CTs on metabolite composition during the aerobic exposure of sainfoin silage via the use of polyethylene glycol (PEG), leading to the inactivation of CTs. (3) Results: The neutral detergent-insoluble protein (NDIP) and acid detergent-insoluble protein concentrations were both more concentrated in the control group than in the PEG-treated group. There were 587 and 651 different metabolites present in the control and PEG-treated groups after 3 and 7 days, respectively, of aerobic exposure of silage. Flavonoids (72 metabolites) were the most abundant among these different metabolites. The addition of PEG upregulated histidine, threonine, asparagine, tryptophan, and glutamine, but downregulated phenylalanine. The relative abundances of Lactococcus, Fructobacillus, Enterobacter, Cutibacterium, Citrobacter, and Rosenbergiella differed significantly between the control and PEG-treated groups (p < 0.05); all of these bacteria showed significant correlation with some of the 50 most abundant metabolites. (4) Conclusions: the results suggest that the antioxidant status of the silage increased and inhibited the activity of a variety of bacteria that coexist with CTs, and decreased the production of certain amino acids after the aerobic exposure of silage.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43127269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Lactobacillus acidophilus—La5 on Composition and Metabolism of the Intestinal Microbiota of Type 2 Diabetics (T2D) and Healthy Individuals Using a Microbiome Model 嗜酸乳杆菌La5对2型糖尿病患者(T2D)和健康个体肠道微生物群组成和代谢的影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-07 DOI: 10.3390/fermentation9080740
M. Salgaço, Fellipe Lopes de Oliveira, A. Sartoratto, V. Mesa, M. Mayer, K. Sivieri
{"title":"Impact of Lactobacillus acidophilus—La5 on Composition and Metabolism of the Intestinal Microbiota of Type 2 Diabetics (T2D) and Healthy Individuals Using a Microbiome Model","authors":"M. Salgaço, Fellipe Lopes de Oliveira, A. Sartoratto, V. Mesa, M. Mayer, K. Sivieri","doi":"10.3390/fermentation9080740","DOIUrl":"https://doi.org/10.3390/fermentation9080740","url":null,"abstract":"Type 2 diabetes is characterized by dysbiosis in the gut, which may lead to systemic inflammation. Therefore, the use of probiotics may help to achieve a balanced microbiota and improve glycemic control. The aim of this study was to verify the impact of Lactobacillus acidophilus—La5 on the gut microbiome of type 2 diabetes adults using the Human Gut Microbial Ecosystem Simulator (SHIME®) and compare this to the microbiome of healthy subjects. Four groups (Control Group: NormoGlycemic; Treatment Group: T2D) were evaluated in SHIME® for 6 weeks. After 7 and 14 days of colonic fermentation, the intestinal microbiota (16S rRNA gene sequencing) and metabolites (short-chain fatty acids) were analyzed. La5 altered the composition of the microbiota after 14 days of treatment for both groups, by increasing the abundance of Bacteroidetes and a decrease in Firmicutes in the NormoGlycemic. Treatment with La5 resulted in a shift in the microbial community of NormoGlycemic with increased abundance of Bacteroides and Mitsuokella and a decrease in Achromobacter and Catabacter, whereas T2D gut microbiome was enriched with Faecalibacterium and reduced in Bacteroides. Megasphaera spp. stimulated with La5 treatment in NormoGlycemic has already been reported to produce intestinal metabolites and recognized to contribute to increased anti-inflammatory and immune responses. Faecalibacterium, on the other hand, can modulate the intestinal epithelium and be a major butyrate product in the microbiota. Finally, this study showed a positive and promising result of La5 treatment in increasing intestinal homeostasis in the microbiota of T2D.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43137591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Dietary Fermented Grape Pomace Supplementation on In Vitro Total Gas and Methane Production, Digestibility, and Rumen Fermentation 日粮添加发酵葡萄渣对体外总气体和甲烷生成、消化率和瘤胃发酵的影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-07 DOI: 10.3390/fermentation9080741
K. Kara, Mehmet Öztaş
{"title":"The Effect of Dietary Fermented Grape Pomace Supplementation on In Vitro Total Gas and Methane Production, Digestibility, and Rumen Fermentation","authors":"K. Kara, Mehmet Öztaş","doi":"10.3390/fermentation9080741","DOIUrl":"https://doi.org/10.3390/fermentation9080741","url":null,"abstract":"The aim of this study comprises the effect of fermented grape pomace (FGP) in experimental total mixed rations (TMR) at different rates (0, 7.5%, 15%, and 22.5%) on the in vitro cumulative gas production (6th, 12th, 18th and 24th hours), methane production, ruminal fermentation values, pH and ammonia-nitrogen and straight and branched short-chain fatty acids (SCFA and BCFA) concentration. The method of in vitro total gas production was carried out in glass syringes. Ruminal in vitro methane production linearly decreased by adding up to 22.5% FGP in experimental TMR (p < 0.05). The molarities of acetic, propionic, butyric, and valeric acids in the in vitro fermentation fluid linearly decreased with the addition of FGP to TMR (p < 0.05). FGP up to 22.5% in experimental TMRs decreased the molarity of iso-valeric acid and iso-butyric acid from BSCFAs (p < 0.05). As a result, it was concluded that the use of FGP, containing a low level of total condensed tannins (TCTs), up to 22.5% in the experimental TMR based on dry matter (DM) did not adversely affect the in vitro ruminal fermentation value and had an anti-methanogenic effect. In addition, some SCFA (acetic, propionic, butyric, and valeric acids) molarities and iso-acid BSCFA (iso-butyric and iso-valeric acid) did not change up to 15% rate of FGP in the ration. Still, these values decreased by using a 22% rate of FGP. The dose-dependent effect of FGP on ruminal iso-acids has been associated with the ability of TCTs to inhibit ruminal protein degradation partially.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44816787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Microbial Community in a Substrate of Solid-State Fermentation by Lentinula edodes: A Preliminary Study 香菇固态发酵底物微生物群落的初步研究
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-06 DOI: 10.3390/fermentation9080736
Lill Eilertsen, M. Hultberg, Natuschka M. Lee, Paramjeet Saroj, M. Swaine, F. Chen, Shaojun Xiong
{"title":"The Microbial Community in a Substrate of Solid-State Fermentation by Lentinula edodes: A Preliminary Study","authors":"Lill Eilertsen, M. Hultberg, Natuschka M. Lee, Paramjeet Saroj, M. Swaine, F. Chen, Shaojun Xiong","doi":"10.3390/fermentation9080736","DOIUrl":"https://doi.org/10.3390/fermentation9080736","url":null,"abstract":"Edible-fungal-based solid-state fermentation holds promise for sustainable food and biofuel production. Understanding the role of microbial communities in fungal substrates is crucial. Birch-based substrates were treated with autoclaving (121 °C, at 2 bar) or hot air pasteurization (75–100 °C), followed by incubation with and without shiitake (Lentinula edodes) inoculum. Mycelial growth was monitored by CO2 release and microbial biomass by phosphate-lipid fatty acid (PLFA). DNA sequencing was used to analyze the microbial communities. Results showed successful colonization of shiitake on all substrates, regardless of pasteurization temperatures and coexisting microbes. Total microbial respiration (CO2) and PLFA biomass showed no significant differences between pasteurization regimes. However, significant microbial differences were found between shiitake-inoculated and non-inoculated treatments. DNA sequencing revealed the dominance of Phyllobacterium, Sphingomonas, and Pelomonas genera in all inoculated substrates, while non-inoculated substrates were abundant in Bacillus spp. and Paenibacillus spp. of the Firmicutes phylum. This study provides preliminary insights into the microbial community in birch-based shiitake substrates, facilitating further investigation of bacteria involved in shiitake mycelium growth promotion and biochemical conversion for biofuel production.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44032569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of Biomass Cultivation from Tuber borchii and Effect of Additives on Triterpenoid Production 硼砂块茎生物量培养的优化及添加剂对三萜产量的影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-06 DOI: 10.3390/fermentation9080735
Yupeng Fu, Parushi Nargotra, Chia-Hung Kuo, Yung-Chuan Liu
{"title":"Optimization of Biomass Cultivation from Tuber borchii and Effect of Additives on Triterpenoid Production","authors":"Yupeng Fu, Parushi Nargotra, Chia-Hung Kuo, Yung-Chuan Liu","doi":"10.3390/fermentation9080735","DOIUrl":"https://doi.org/10.3390/fermentation9080735","url":null,"abstract":"Edible fungi are renowned for producing biologically active secondary metabolites that possess anti-tumor activity, protect the liver and have other benefits. The cultivation of truffle mycelia through submerged fermentation has gained interest in the production of metabolites for bio-medicinal purposes. In the present study, Tuber borchii was cultivated by submerged fermentation to produce both biomass and triterpenoids. Various additives, including palmitic acid, stearic acid, linoleic acid, chitosan, CaCl2 and limonene, were investigated to enhance triterpenoid production. It was observed that increasing the medium’s linoleic acid concentration to 1 g/L increased the production of triterpenoids to 129.29 ± 6.5 mg/L, which was 2.94 times higher than the control. A number of variables, including potassium and magnesium ion concentrations and carbon and nitrogen sources and concentrations, were considered to ascertain the ideal conditions for T. borchii growth in submerged fermentation. The best concentrations for glucose, yeast extract, peptone, malt extract, KH2PO4 and MgSO4·7H2O in submerged fermentation were 19.45, 4.58, 7.91, 5.3, 0.58 and 0.82 g/L, respectively, according to response surface methodology. Validation analysis revealed that the experimental values and the predicted values were in good agreement. Under ideal circumstances, the maximum dry cell weight (2.980.18 g/L), which was 1.39 times greater than the control, was attained. Finally, the addition of 1.5 g/L linoleic acid on day 14 to the optimal medium elevated the triterpenoid production to 212.63 ± 16.58 mg/L, which was a 4.84-fold increase compared to the control.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45710639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic Engineering of Microorganisms to Produce L-Aspartate and Its Derivatives l -天冬氨酸及其衍生物微生物代谢工程研究
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-06 DOI: 10.3390/fermentation9080737
A. Shi, Yan Liu, Baolei Jia, Gang Zheng, Yanlai Yao
{"title":"Metabolic Engineering of Microorganisms to Produce L-Aspartate and Its Derivatives","authors":"A. Shi, Yan Liu, Baolei Jia, Gang Zheng, Yanlai Yao","doi":"10.3390/fermentation9080737","DOIUrl":"https://doi.org/10.3390/fermentation9080737","url":null,"abstract":"Metabolic engineering is a promising strategy to realize green synthesis of valued chemicals derived from petroleum. According to the literature, cell factories for producing L-aspartate and its derivatives (β-alanine, ectoine, 3-hydroxypropionate, D-pantothenic acid and L-homoserine) have been developed. In this review, we firstly introduced the functions, applications and markets of L-aspartate and its derivatives. Then, the current research progress on microbial production of them was elaborated in detail. Finally, we have discussed the limiting factors and given some suggestions for realizing applications of engineered bacteria in the industry, including metabolic engineering of the bacteria to increase the titer, yield and productivity of the target products, fermentation condition optimization and downstream purification. With the development of novel technologies and increased investments in synthetic biology, it is promising to realize sustainable production of L-aspartate and its derivatives at the industrial scale in the future.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44503949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Solid State and Semi-Solid Fermentations of Olive and Sunflower Cakes with Yarrowia lipolytica: Impact of Biological and Physical Pretreatments 用解脂亚罗维菌固态和半固态发酵橄榄和向日葵蛋糕:生物和物理预处理的影响
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-06 DOI: 10.3390/fermentation9080734
A. R. Costa, H. Fernandes, J. Salgado, I. Belo
{"title":"Solid State and Semi-Solid Fermentations of Olive and Sunflower Cakes with Yarrowia lipolytica: Impact of Biological and Physical Pretreatments","authors":"A. R. Costa, H. Fernandes, J. Salgado, I. Belo","doi":"10.3390/fermentation9080734","DOIUrl":"https://doi.org/10.3390/fermentation9080734","url":null,"abstract":"Lignocellulosic biomass is a promising feedstock for added value compound production in biotechnological processes such as solid-state fermentation (SSF). Although these solid materials can be directly used as substrates in fermentations in a solid state, a pretreatment is often required, especially if the microorganism selected is unable to produce lignocellulosic enzymes. In the present work, several pretreatment strategies were applied to a 50% (w/w) mixture of olive and sunflower cakes before SSF for lipase production by the oleaginous yeast Yarrowia lipolytica W29. Co-culture strategies with Y. lipolytica and Aspergillus niger did not improve lipase production by the oleaginous yeast. Biological pretreatment with a fungal enzymatic extract led to a significant increase in sugar availability in the substrate mixture after a short incubation period, improving yeast growth. Microwave and ultrasound were the physical pretreatments selected and microwave irradiation proved to be the best method, resulting in 44% and 17% increases in yeast growth and lipase production, respectively, compared to the untreated mixture. An improvement in lipase activity was also observed after ultrasonic treatment in semi-solid fermentations, leading to a 2-fold increase in this enzyme activity compared to the control. The utilization of pretreatments before SSF with Y. lipolytica can increase sugars availability and result in structural changes in the solid substrate, which can improve the bioprocesses’ productivity.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47420544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Dynamics of Physicochemical Properties, Flavor, and Microbial Communities of Salt-Free Bamboo Shoots during Natural Fermentation: Correlation between Microorganisms and Metabolites 无盐竹笋自然发酵过程中理化性质、风味和微生物群落的动态:微生物与代谢产物的相关性
IF 3.7 3区 农林科学
Fermentation-Basel Pub Date : 2023-08-06 DOI: 10.3390/fermentation9080733
Xiaofeng Xu, Zhijian Long, Wanning Du, Qiyang Chen, Yu Zhang, Shanglian Hu
{"title":"Dynamics of Physicochemical Properties, Flavor, and Microbial Communities of Salt-Free Bamboo Shoots during Natural Fermentation: Correlation between Microorganisms and Metabolites","authors":"Xiaofeng Xu, Zhijian Long, Wanning Du, Qiyang Chen, Yu Zhang, Shanglian Hu","doi":"10.3390/fermentation9080733","DOIUrl":"https://doi.org/10.3390/fermentation9080733","url":null,"abstract":"Sour bamboo shoot is a Chinese fermented vegetable with unique flavors and is favored by local consumers. In this study, at different fermentation times, the texture of bamboo shoots and the changing rules of pH, titratable acid (TA), reduced sugar, and nitrite in bamboo shoot fermentation broth were explored. Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to investigate the dominant aroma compounds. 16S rRNA high-throughput sequencing technology (HTS) was employed to investigate the core microbial communities. The results show that the chewiness, fracturability, hardness, and pH decreased, while TA increased during the 60-day fermentation. The contents of reducing sugar and nitrite peaked on the 14th day of fermentation and then decreased. A total of 80 volatile compounds were detected during sour bamboo shoot fermentation, with 2,4-Di-tert-butylphenol having the highest concentration. Among them, 12 volatile compounds (VIP ≥ 1) were identified as characteristic aroma substances of sour bamboo shoots. The dominant bacterial phyla in sour bamboo shoots were Firmicutes and Proteobacteria, while Bacillus and Acinetobacter were the dominant genus. Correlation analysis showed that Firmicutes exhibited a positive correlation with 3,6-Nonadien-1-ol, (E,Z)-, Oxalic acid, isobutyl hexyl ester, and (-)-O-Acetylmalic anhydride, whereas Bacillus exhibited a negative correlation with Silanediol, dimethyl-, and Oxime-, methoxy-phenyl-. A detailed picture of the microbial community of fermented bamboo shoots has been provided by this study, and it may provide insight into the Chinese traditional fermented vegetable microbial structure.","PeriodicalId":48535,"journal":{"name":"Fermentation-Basel","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47803557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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