Insect Systematics and Diversity最新文献_第3页

Phylogeography and species delimitation of the Asian cavity-nesting honeybees 亚洲腔巢蜜蜂的系统地理学与种界

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-07-01 DOI: 10.1093/isd/ixad015

Yong-Chao Su, Yi-Fan Chiu, Natapot Warrit, G. Otis, D. Smith

引用次数: 1

Prey-associated genetic differentiation in two species of silver fly (Diptera: Chamaemyiidae), Leucotaraxis argenticollis and L. piniperda 银蝇(双翅目:银蝇科)和银蝇的猎物相关遗传分化

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-05-01 DOI: 10.1093/isd/ixad007

N. Havill, Tonya D. Bittner, J. Andersen, Nicholas J. Dietschler, J. Elkinton, S. Gaimari, Brian P. Griffin, Deanna Zembrzuski, M. Whitmore

{"title":"Prey-associated genetic differentiation in two species of silver fly (Diptera: Chamaemyiidae), Leucotaraxis argenticollis and L. piniperda","authors":"N. Havill, Tonya D. Bittner, J. Andersen, Nicholas J. Dietschler, J. Elkinton, S. Gaimari, Brian P. Griffin, Deanna Zembrzuski, M. Whitmore","doi":"10.1093/isd/ixad007","DOIUrl":"https://doi.org/10.1093/isd/ixad007","url":null,"abstract":"\u0000 Sympatric host-associated genetic differentiation is a prominent pattern that could lead to speciation. In insects, there are numerous examples of host-associated differentiation among herbivores that prefer different plants, and parasitoids that prefer different hosts, but few examples for specialist predators. We developed new microsatellite loci for two species of silver fly, Leucotaraxis argenticollis (Zetterstedt) and L. piniperda (Malloch) (Diptera: Chamaemyiidae), being evaluated as biological control agents for the hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), in eastern North America where it is a nonnative pest. We obtained DNA from specimens of both fly species feeding on native A. tsugae in western North America, as well as on other western and eastern adelgid species. We performed population genetic analyses using the new loci and DNA barcode sequences. Our results confirmed east–west allopatric divergence and uncovered nested genetic differentiation associated with different adelgid prey species and their host plants in western North America for both species of silver flies. For both species, there is also evidence for a longer history of diversification in the west, with ancestral specialization of feeding on pine adelgids, which was retained after range expansion to the east. More recently, divergence to feeding on new adelgid prey species occurred in the west. Our findings are consistent with the hypothesis that host-alternating life cycles in Adelgidae may provide temporary escape from specialist predators. We discuss the implications for biological control efficacy and potential for lineage hybridization as western flies are released in the east to control A. tsugae.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48010405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A relict subterranean spider (Araneae: Linyphiidae: Troglohyphantes) reveals a unique component of the biogeography of Corsica 一只残存的地下蜘蛛(蜘蛛目:林蛛科:穴居蛛)揭示了科西嘉岛生物地理的一个独特组成部分

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-05-01 DOI: 10.1093/isd/ixad008

M. Isaia, S. Mammola, M. Arnedo

{"title":"A relict subterranean spider (Araneae: Linyphiidae: Troglohyphantes) reveals a unique component of the biogeography of Corsica","authors":"M. Isaia, S. Mammola, M. Arnedo","doi":"10.1093/isd/ixad008","DOIUrl":"https://doi.org/10.1093/isd/ixad008","url":null,"abstract":"\u0000 Spiders of the genus Troglohyphantes (Araneae: Linyphiidae) exemplify one of the largest subterranean adaptive radiation across European mountain ranges, counting over 130 species and representing about one fifth of total species richness of cave spiders in Europe. Despite the emerging potential of Troglohyphantes as a biogeographical model, no attempt has been made to reconstruct the geological events underlying the current distribution patterns of the genus. By coupling traditional taxonomy with target gene sequence data and comparative functional trait analyses, we describe a new species of Troglohyphantes, the first reported from the island of Corsica (France). The species is characterized by a high level of subterranean adaptation and distinct morphological affinities with geographically distant congenerics. By means of time-stamped phylogenies, we tested contrasting hypotheses about the origin of the new species. The most parsimonious explanation suggests that the species diverged from an ancestral group of species originating in the Adriatic plate in the lower Miocene (~19 Ma), colonizing Corsica from the east. In the absence of relevant fossil records, the well-known geochronology of Corsica and the Western Mediterranean basin can be used in future studies to reconstruct the biogeography of the whole genus and for inferring the timeline of its diversification.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42362045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reviewers for Insect Systematics and Diversity (November 2021–October 2022) 昆虫分类学和多样性审稿人(2021年11月- 2022年10月)

1区农林科学

Insect Systematics and Diversity Pub Date : 2023-05-01 DOI: 10.1093/isd/ixad001

引用次数: 0

Assessment of targeted enrichment locus capture across time and museums using odonate specimens 使用牙形石标本评估跨时间和博物馆的靶向富集位点捕获

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-05-01 DOI: 10.1093/isd/ixad011

Aaron M. Goodman, Ethan Tolman, R. Uche-Dike, J. Abbott, Jesse W. Breinholt, S. Bybee, P. Frandsen, J. Gosnell, R. Guralnick, V. Kalkman, M. Kohli, Judicael Fomekong Lontchi, Pungki Lupiyaningdyah, Lacie G Newton, J. Ware

{"title":"Assessment of targeted enrichment locus capture across time and museums using odonate specimens","authors":"Aaron M. Goodman, Ethan Tolman, R. Uche-Dike, J. Abbott, Jesse W. Breinholt, S. Bybee, P. Frandsen, J. Gosnell, R. Guralnick, V. Kalkman, M. Kohli, Judicael Fomekong Lontchi, Pungki Lupiyaningdyah, Lacie G Newton, J. Ware","doi":"10.1093/isd/ixad011","DOIUrl":"https://doi.org/10.1093/isd/ixad011","url":null,"abstract":"\u0000 The use of gDNAs isolated from museum specimens for high throughput sequencing, especially targeted sequencing in the context of phylogenetics, is a common practice. Yet, little understanding has been focused on comparing the quality of DNA and results of sequencing museum DNAs. Dragonflies and damselflies are ubiquitous in freshwater ecosystems and are commonly collected and preserved insects in museum collections hence their use in this study. However, the history of odonate preservation across time and museums has resulted in wide variability in the success of viable DNA extraction, necessitating an assessment of their usefulness in genetic studies. Using Anchored Hybrid Enrichment probes, we sequenced DNA from samples at 2 museums, 48 from the American Museum of Natural History (AMNH) in NYC, USA and 46 from the Naturalis Biodiversity Center (RMNH) in Leiden, Netherlands ranging from global collection localities and across a 120-year time span. We recovered at least 4 loci out of an >1,000 locus probe set for all samples, with the average capture being ~385 loci (539 loci on average when a clade of ambiguous taxa omitted). Neither specimen age nor size was a good predictor of locus capture, but recapture rates differed significantly between museums. Samples from the AMNH had lower overall locus capture than the RMNH, perhaps due to differences in specimen storage over time.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47906127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A molecular phylogeny of Noctuini (Lepidoptera: Noctuidae: Noctuinae) Noctuini的分子系统发育（鳞翅目：Noctuinae）

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-05-01 DOI: 10.1093/isd/ixad009

Melissa S Sisson, Matthew J Flom, Janna L Crossley, Rebecca Simmons

{"title":"A molecular phylogeny of Noctuini (Lepidoptera: Noctuidae: Noctuinae)","authors":"Melissa S Sisson, Matthew J Flom, Janna L Crossley, Rebecca Simmons","doi":"10.1093/isd/ixad009","DOIUrl":"https://doi.org/10.1093/isd/ixad009","url":null,"abstract":"\u0000 The tribe Noctuini is comprised over 520 species; many are economically important species that impact human agriculture. Despite their diversity and relevance, relationships of Noctuini have been difficult to resolve. There have been extensive morphological revisions of some of these taxa (e.g., Agrotis Ochsenheimer, Euxoa Hübner); however, there are no studies that focus exclusively on the phylogenetic relationships of the Noctuini. Currently, Noctuini are separated into 2 subtribes, Agrotina and Noctuina. In this study, we tested previous classification schemes with 2 analyses. The total evidence analysis utilized 3 loci for 3 outgroup taxa and 54 noctuine species. Using museum specimens, we collected data from cytochrome oxidase I (COI), Dopa Decarboxylase (DDC), and Elongation factor 1-α (EF1-α) to generate a dataset of 1,378 base pairs (bp) for a total evidence approach. We also used previously published COI sequences for 626 species, resulting in a matrix of 690 bp, termed “the barcode analysis.” We used both Maximum Likelihood and Bayesian approaches for the total evidence analysis, and ML for the barcode analysis. In both sets of results, we found equivocal support for monophyletic Agrotina and Noctuina. We found strong support for smaller, well-described genera (e.g., Spaelotis), but poor support for large genera such as Euxoa, Feltia Walker, and Agrotis. It is likely that a combination of limited taxon/gene sampling, limited gene choice, and the rapid evolution of these species resulted in a lack of phylogenetic resolution in the total evidence topologies. Based on these results, we recommend increased taxon sampling and inclusion of loci that target these rapidly evolving lineages to achieve a better understanding of the phylogeny of Noctuini and its genera.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44274368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenomics reveals within species diversification but incongruence with color phenotypes in widespread orchid bees (Hymenoptera: Apidae: Euglossini) 系统基因组学揭示了广泛分布的兰蜂在种内多样化但与颜色表型不一致(膜翅目:蜂科:蓝蜂)

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-03-01 DOI: 10.1093/isd/ixad005

S. Sandoval-Arango, M. Branstetter, Carolina F. Cardoso, M. López-Uribe

{"title":"Phylogenomics reveals within species diversification but incongruence with color phenotypes in widespread orchid bees (Hymenoptera: Apidae: Euglossini)","authors":"S. Sandoval-Arango, M. Branstetter, Carolina F. Cardoso, M. López-Uribe","doi":"10.1093/isd/ixad005","DOIUrl":"https://doi.org/10.1093/isd/ixad005","url":null,"abstract":"Coloration is an important phenotypic trait for taxonomic studies and has been widely used for identifying insect species and populations. However, coloration can be a poor diagnostic character for insect species that exhibit high polymorphism in this trait, which can lead to over-splitting of taxonomic units. In orchid bees, color variation has been interpreted by different taxonomists as either polymorphism associated with Müllerian mimicry complexes or diagnostic traits for species identification. Despite this uncertainty, integrative approaches that incorporate multiple independent datasets to test the validity of hair coloration as a character that identifies independent evolutionary units have not been used. Here, we use phylogenomic data from Ultraconserved Elements (UCEs) to explore whether color phenotypes in the widespread orchid bee species complexes Eulaema meriana and Eulaema bombiformis (Hymenoptera: Apidae: Euglossini) correspond to independent lineages or polymorphic trait variation within species. We find that lineages within both species are structured according to geography and that color morphs are generally unassociated with evolutionarily independent groups except for populations located in the Atlantic Forest of Brazil. We conclude that there is compelling evidence that E. atleticana and E. niveofasciata are subspecies of E. meriana and E. bombiformis, respectively, and not different species as previously suggested. Therefore, we recognize Eulaema meriana atleticana comb. n. and Eulaema bombiformis niveofasciata comb. n. and discuss their morphological characteristics. We make recommendations on the use of color traits for orchid bee taxonomy and discuss the significance of subspecies as evolutionary units relevant for conservation efforts.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46382022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic systematics, diversification, and biogeography of Cerurinae (Lepidoptera: Notodontidae) and a description of a new genus Cerurinae的系统发育系统学、多样性和生物地理学（鳞翅目：Notodontidae）及一个新属的描述

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-03-01 DOI: 10.1093/isd/ixad004

Ryan A. St Laurent, Paul Z. Goldstein, James S. Miller, A. Markee, Hermann S. Staude, A. Kawahara, Scott E. Miller, Robert K. Robbins

{"title":"Phylogenetic systematics, diversification, and biogeography of Cerurinae (Lepidoptera: Notodontidae) and a description of a new genus","authors":"Ryan A. St Laurent, Paul Z. Goldstein, James S. Miller, A. Markee, Hermann S. Staude, A. Kawahara, Scott E. Miller, Robert K. Robbins","doi":"10.1093/isd/ixad004","DOIUrl":"https://doi.org/10.1093/isd/ixad004","url":null,"abstract":"We present the first dated molecular phylogeny of the Cerurinae moths (Notodontidae), based on sequence data for 666 loci generated by anchored hybrid enrichment. Monophyly of Cerurinae is corroborated, which includes the following genera: Pararethona Janse, Pseudorethona Janse, Oreocerura Kiriakoff, stat. rev., Cerurella Kiriakoff, Notocerura Kiriakoff, Hampsonita Kiriakoff, Afrocerura Kiriakoff, Cerurina Kiriakoff, Neoharpyia Daniel, Furcula Lamarck, Neocerura Matsumura, Americerura St Laurent and Goldstein, gen. nov., Cerura Schrank, and Kamalia Koçak & Kemal. The type species of the Neotropical genus Tecmessa Burmeister, T. annulipes (Berg), which had been incorrectly assigned to Cerurinae, is recovered in Heterocampinae; and Americerura gen. nov. is proposed to receive 17 unambiguously cerurine species transferred from Tecmessa. Divergence time estimates recover a crown age of Notodontidae roughly coincident with the K-Pg boundary, and a late-Oligocene crown age for Cerurinae. An African origin is inferred for Cerurinae, followed by colonization of the Palearctic, the Americas, Indomalaya, and Australasia during the Miocene. At least three independent colonizations of the Americas are inferred, one in the mid-Miocene associated with ancestral Americerura gen. nov. and two in the Pliocene and Pleistocene within Furcula. We hypothesize that the global spread of Cerurinae was enabled by that of its primary caterpillar foodplants in the Salicaceae. State-dependent diversification analyses suggest that cerurines diversified most rapidly in temperate climates.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42360938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

‘Social Glands’ in Parasitoids? – Convergent Evolution of Metapleural Glands in Hymenoptera 寄生虫的“社会一瞥”膜翅目下胸膜腺的聚合进化

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-03-01 DOI: 10.1093/isd/ixad006

Jonah M. Ulmer, I. Mikó, A. Richter, A. Helms, T. V. D. Kamp, L. Krogmann

{"title":"‘Social Glands’ in Parasitoids? – Convergent Evolution of Metapleural Glands in Hymenoptera","authors":"Jonah M. Ulmer, I. Mikó, A. Richter, A. Helms, T. V. D. Kamp, L. Krogmann","doi":"10.1093/isd/ixad006","DOIUrl":"https://doi.org/10.1093/isd/ixad006","url":null,"abstract":"For over a century, the metapleural gland, an exocrine gland above the hind coxa, has been thought to be a unique structure for ants (Hymenoptera: Formicidae), and regarded as a catalyst for the ecological and evolutionary success of the family. This gland is one of the most researched exocrine glands in arthropods and its anatomy, ultrastructure, and chemistry are well documented. Herein, we describe an exocrine gland from the proctotrupoid wasp Pelecinus polyturator (Hymenoptera: Pelecinidae) with a similar position, structure, and chemistry to the ant metapleural gland: it is located just above the hind coxa, corresponds to an externally concave and fenestrated atrium, is composed of class 3 gland cells, and its extract contains relatively strong acids. We discover that the pelecinid gland is associated with the dilator muscle of the first abdominal spiracle, a trait that is shared with ants, but remained overlooked, possibly due to its small diameter, or obfuscation by the extensive metapleural gland. We also provide a biomechanical argument for passive emptying of the gland in both taxa. Pelecinids and ants with metapleural glands share a close association with soil. The pelecinid metapleural gland might therefore also have an antiseptic function as suggested for ants. We examined 44 other Hymenoptera families and found no glands associated with the oclusor apodeme or any signs of external modification. Our results strongly indicate that this complex trait (anatomical & chemical) evolved independently in ants and pelecinid wasps providing an exceptional system to better understand exocrine gland evolution in Hymenoptera.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45949199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Genetic Characterization of Single Cohort Adult Colonies With Male Aggregations, and Preliminary Evidence for Lekking in a Malagasy Kite Spider (Isoxya, Gasteracanthinae) 马达加斯加鸢蛛(Isoxya, Gasteracanthinae)单群成年雄蛛群体的发现、遗传特征及Lekking的初步证据

IF 3.4 1区农林科学

Insect Systematics and Diversity Pub Date : 2023-01-01 DOI: 10.1093/isd/ixac029

I. Agnarsson, James Starrett, Zachary Babbitz, J. Bond, Matjaž Gregorič, Onjaherizo Christian Raberahona, Steven Williams, Matjaž Kuntner

{"title":"Discovery and Genetic Characterization of Single Cohort Adult Colonies With Male Aggregations, and Preliminary Evidence for Lekking in a Malagasy Kite Spider (Isoxya, Gasteracanthinae)","authors":"I. Agnarsson, James Starrett, Zachary Babbitz, J. Bond, Matjaž Gregorič, Onjaherizo Christian Raberahona, Steven Williams, Matjaž Kuntner","doi":"10.1093/isd/ixac029","DOIUrl":"https://doi.org/10.1093/isd/ixac029","url":null,"abstract":"Abstract Spiders are notoriously solitary and cannibalistic, with instances of colonial or social lifestyles in only about 50-60, or ∼0.1% of 50,000 described species. Population analyses indicate that most colonies consist of multiple cohorts formed by close relatives.Territorial social spiders facultatively form colonies by interlinking individual webs, but further cooperation is infrequent, and only among juveniles or (rarely) females. In spiders therefore, aggregations of males outside of the male-male competition context has been unknown. Here, we report on a discovery of a kite spider from Madagascar that exhibits unique colonies. We found colonies of the newly described araneid Isoxya manangona n. sp. formed by up to 41 interconnected, single-cohort adult female webs with up to 38 adult males aggregating on a central, single, nonsticky line. With males resting tightly together, we found no evidence for male-male aggression. Genetic analyses from RAD sequencing suggest that most colonies consist of unrelated individuals. Furthermore, genetic variability of males was somewhat less than that of females. Single cohort colonies made up purely of adults, and peaceful male aggregations, have not previously been observed in spiders. Although direct behavioral observations are preliminary, we speculate based on the available evidence that these colonies may represent a novel and first case of lekking in spiders.","PeriodicalId":48498,"journal":{"name":"Insect Systematics and Diversity","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61455806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0