IP archives of cytology and histopathology research最新文献_第4页

Clinical and cytohistomorphological evaluation of peripheral nerve sheath tumours with special reference to immunohistochemical diversity in a tertiary care hospital 三级医院外周神经鞘肿瘤的临床和细胞组织形态学评价，特别参考免疫组织化学多样性

IP archives of cytology and histopathology research Pub Date : 2023-10-15 DOI: 10.18231/j.achr.2023.043

Asfa Shams, Veena Maheshwari, Nishat Afroz

{"title":"Clinical and cytohistomorphological evaluation of peripheral nerve sheath tumours with special reference to immunohistochemical diversity in a tertiary care hospital","authors":"Asfa Shams, Veena Maheshwari, Nishat Afroz","doi":"10.18231/j.achr.2023.043","DOIUrl":"https://doi.org/10.18231/j.achr.2023.043","url":null,"abstract":"Peripheral Nerve Sheath Tumours (PNST), though have a frequent prevalence, are diagnostically challenging. There is a wide spectrum of lesions ranging from benign entities like neurofibroma, Schwannoma, perineurioma to malignant entities like malignant peripheral nerve sheath tumours (MPNST), malignant granular cell tumour and malignant perineurioma. Since there is an overlap with other spindle and round cell tumours, IHC and molecular studies are needed to provide a clear distinction between different tumour types. It was a 5 years observational study (3 years retrospective and 2 years prospective) conducted in the Department of Pathology, JNMCH, AMU from 2015-2020. . History and relevant clinical findings were retrieved from the archives of both the Histopathology and Cytopathology Lab. Imprints as well as FNA smears were included from Cytopathology Lab and biopsies and resection specimen from Histopathology Lab. Immunohistochemistry was applied wherever necessary. A total of 82 cases were diagnosed over a 5 year period out of which 70 were benign and 12 malignant. Male preponderance was seen. Benign tumours comprised mostly of Neurofibroma and Schwannoma and there were 12 cases of MPNST. Correlation was established between Cytopathological and Histopathological findings and IHC, mostly S100 proved useful in differentiating it from other malignant spindle cell tumours. The differential diagnoses of these tumours has always posed a problem given their monotonous appearance and clinical overlap. This study, therefore, aims to analyse the diverse morphological features of Peripheral Nerve Sheath Tumours in the light of cytopathological and histopathological findings and immunohistochemistry.","PeriodicalId":479918,"journal":{"name":"IP archives of cytology and histopathology research","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of programmed cell death ligand- 1 (PD-L1) expression in oral potentially malignant disorders and oral squamous cell carcinoma - An immunohistochemical study 评估程序性细胞死亡配体- 1 (PD-L1)在口腔潜在恶性疾病和口腔鳞状细胞癌中的表达-一项免疫组织化学研究

IP archives of cytology and histopathology research Pub Date : 2023-10-15 DOI: 10.18231/j.achr.2023.040

Priyanka S Pachpande, Mandakini S Mandale, Jyoti D Bhavthankar, Jayanti G Humbe, Poonam Zanwar

{"title":"Assessment of programmed cell death ligand- 1 (PD-L1) expression in oral potentially malignant disorders and oral squamous cell carcinoma - An immunohistochemical study","authors":"Priyanka S Pachpande, Mandakini S Mandale, Jyoti D Bhavthankar, Jayanti G Humbe, Poonam Zanwar","doi":"10.18231/j.achr.2023.040","DOIUrl":"https://doi.org/10.18231/j.achr.2023.040","url":null,"abstract":"OSCC is multifactorial and is a multistep process. It may arise de-novo or may be preceded by oral potentially malignant disorders(OPMD). During malignant transformation certain molecular changes are evident at cellular level even in the absence of phenotypic changes in the tissue. With the recent advances in molecular understanding of cancers, immune checkpoint inhibitors have been recognized as perpetrators or supporters in the pathophysiology of various cancers. One of the main immunosuppressive pathways is the programmed death 1 (PD-1)/programmed death-ligand 1 (PD-L1) in which there is an interaction between T-cell PD-1 receptor and PD-L1 on cancer cells. Upregulation of PD-L1 is associated with disease progression. To assess and compare the PD-L1 expression in OPMD and OSCC. 64 Paraffin embedded tissue sections of histopathologically diagnosed cases of 32 OSCC and 32 OPMD were immunohistochemically stained with PD-L1 & its membranous expression was evaluated. Descriptive statistical analysis was applied. Thus the study showed 100 % PD-L1 positive expression in OSCC and PD-L1 expression increased with increase in histopathological. In case of OPMD 93.74 % showed positive PD-L1 expression. We found PDL1 expression is significantly higher in OSCC (Mean= 4.59 ± 1.965) compared to OPMD (Mean= 2.03 ± 1.204). Assessment of PD-L1 expression in OPMD patients will help us to screen the subjects with or without risk of malignant transformation as increase in PD-L1 expression signifies the increased risk of malignant change.","PeriodicalId":479918,"journal":{"name":"IP archives of cytology and histopathology research","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reporting breast fine needle aspiration cytology using the international academy of cytology Yokohama system: Experience in a tertiary care centre 报告乳房细针抽吸细胞学使用国际学院细胞学横滨系统:经验在三级护理中心

IP archives of cytology and histopathology research Pub Date : 2023-10-15 DOI: 10.18231/j.achr.2023.039

Karthik Dhandapani, Sunitha Shankaralingappa, Trupti Patel, Sangita Vanik, B R Nagarjun, Udaya Sundarajan

{"title":"Reporting breast fine needle aspiration cytology using the international academy of cytology Yokohama system: Experience in a tertiary care centre","authors":"Karthik Dhandapani, Sunitha Shankaralingappa, Trupti Patel, Sangita Vanik, B R Nagarjun, Udaya Sundarajan","doi":"10.18231/j.achr.2023.039","DOIUrl":"https://doi.org/10.18231/j.achr.2023.039","url":null,"abstract":": In developing countries for all palpable breast lesions, Fine needle aspiration Biopsy (FNAB) is the most suitable test as FNAB is minimally invasive and cost-effective. The present study was undertaken to categorise the Breast Fine Needle Aspiration Biopsy (FNAB) samples according to the IAC Yokohama system of reporting and to assess the Risk of malignancy (ROM) for each category as well as to elucidate other quality indicators of the Breast FNAB. : Among the total 102 cases in which FNAC were performed, 9 were insufficient/ inadequate (8.80%), 11 were benign (10.80%), 5 were atypical (4.95%), 3 were suspicious (2.95%) and the rest 74 FNACs were malignant (72.5%). ROM of insufficient/inadequate, benign, atypical, suspicious and malignant ware 37.5%, 0%, 50%, 100% and 100% respectively. Absolute sensitivity (only category V) was 90.1 and complete sensitivity (including category III to V) was 96.3%. Specificity of IAC Yokohama system is 100% when considering category 5 as positive and is 88.9% when considering category 3 to 5 as positive. : The IAC Yokohama Reporting System for breast cytology has high sensitivity, specificity. The ROM in our study done in Indian population ware comparable to the proposed IAC Yokohama system which suggests the IAC Yokohama system has high reproducibility and can be applied in Indian population. Usage of FNAC for the evaluation of breast lumps and categorization based on the Yokohama system helps in ideal management of the patient, reducing the requirement of core needle biopsy.","PeriodicalId":479918,"journal":{"name":"IP archives of cytology and histopathology research","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precursor lesions of lung tumors 肺肿瘤的前驱病变

IP archives of cytology and histopathology research Pub Date : 2023-10-15 DOI: 10.18231/j.achr.2023.036

Indranil Chakrabarti, Sujaya Mazumder

引用次数: 0

Assessment of immunohistochemical expression of claudin-1 in oral squamous cell carcinoma and its clinicopathological correlation claudin-1在口腔鳞状细胞癌中的免疫组化表达及临床病理相关性研究

IP archives of cytology and histopathology research Pub Date : 2023-10-15 DOI: 10.18231/j.achr.2023.038

Poonam Rajendra Zanwar, Jayanti Govind Humbe, Jyoti Dilip Bhavthankar, Mandakini Subhash Mandale, Priyanka Sanjay Pachpande

{"title":"Assessment of immunohistochemical expression of claudin-1 in oral squamous cell carcinoma and its clinicopathological correlation","authors":"Poonam Rajendra Zanwar, Jayanti Govind Humbe, Jyoti Dilip Bhavthankar, Mandakini Subhash Mandale, Priyanka Sanjay Pachpande","doi":"10.18231/j.achr.2023.038","DOIUrl":"https://doi.org/10.18231/j.achr.2023.038","url":null,"abstract":"Oral carcinogenesis is complex and multi-step process, which results from various deleterious habits, multiple environmental factors and genetic susceptibility. CLDN-1 expression is regulated oncogenic Wnt/B-catenin transduction pathway. They recruit matrix metalloproteinases (MMPs) on the cell surface to achieve elevated focal concentrations and eventual activations of proMMP2. These collagenases are responsible for the breakdown of extracellular matrix proteins and thus facilitate invasion and spread of malignant cells. Reduced cell-cell adhesion is associated with loss of contact inhibition of proliferation. This allows escape from growth control signals and triggers carcinogenesis. Significant correlation was observed between histopathological grade of the tumor with the localization and immunostaining intensity of CLDN-1. Determination of localization of CLDN-1 for a particular patient may be important to decide site (cytoplasmic or nuclear) for targeting CLDN-1. This targeted drug therapy for CLDN-1 may prevent worsening of the disease in the patient, resulting in better prognosis.","PeriodicalId":479918,"journal":{"name":"IP archives of cytology and histopathology research","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0