Journal of Microbiology & Biology Education最新文献_第7页

Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students. 酵母重组克隆多酮类化合物的异源生物合成：本科生分子微生物学实验模块。

IF 1.9

Journal of Microbiology & Biology Education Pub Date : 2023-11-27 eCollection Date: 2023-12-01 DOI: 10.1128/jmbe.00242-22

Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen

{"title":"Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students.","authors":"Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen","doi":"10.1128/jmbe.00242-22","DOIUrl":"https://doi.org/10.1128/jmbe.00242-22","url":null,"abstract":"Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes. Among the multitude of available plasmid assembly strategies, in vivo homologous recombinational cloning in yeast has emerged as a cost-effective and relatively simple method. Since we use this method routinely in our group for assembling large plasmids with secondary metabolite gene clusters and for direct heterologous production of polyketides in Saccharomyces cerevisiae, we developed an exercise module for undergraduate students where they would get hands-on experience with these molecular practices. The exercises target several molecular techniques, including PCR, restriction enzyme digestion, and yeast recombinational cloning. The students will learn about plasmid assembly and yeast transformation methods by performing these experiments while inherently acquiring new skills valuable for their subsequent laboratory work or projects.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"24 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10720416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting student performance with inclusive writing-to-learn assignments through graphic organizers in large enrollment undergraduate biology courses. 在大量入学的本科生物课程中，通过图形组织器布置包容性的 "以写促学 "作业，提高学生的学习成绩。

IF 1.9

Journal of Microbiology & Biology Education Pub Date : 2023-11-21 eCollection Date: 2023-12-01 DOI: 10.1128/jmbe.00087-23

Alison Wallace, Theresa Barosh, Ellen Brisch, Paul Laybourn, Meena M Balgopal

引用次数: 0

Engaging students in a genetics course-based undergraduate research experience utilizing Caenorhabditis elegans in hybrid learning to explore human disease gene variants. 让学生参与以遗传学课程为基础的本科生研究体验，在混合学习中利用秀丽隐杆线虫探索人类疾病基因变异。

IF 1.9

Journal of Microbiology & Biology Education Pub Date : 2023-11-16 eCollection Date: 2023-12-01 DOI: 10.1128/jmbe.00078-23

Natalie R Forte, Virginia N Veasey, Bethany J Christie, Amira Carter, Marli A Hanks, Alan Holderfield, Taylor Houston, Anil K Challa, Ashley N Turner

{"title":"Engaging students in a genetics course-based undergraduate research experience utilizing Caenorhabditis elegans in hybrid learning to explore human disease gene variants.","authors":"Natalie R Forte, Virginia N Veasey, Bethany J Christie, Amira Carter, Marli A Hanks, Alan Holderfield, Taylor Houston, Anil K Challa, Ashley N Turner","doi":"10.1128/jmbe.00078-23","DOIUrl":"https://doi.org/10.1128/jmbe.00078-23","url":null,"abstract":"Genetic analysis in model systems using bioinformatic approaches provides a rich context for a concrete and conceptual understanding of gene structure and function. With the intent to engage students in research and explore disease biology utilizing the nematode Caenorhabditis elegans model, we developed a semester-long course-based undergraduate research experience (CURE) in a hybrid (online/in-person) learning environment-the gene-editing and evolutionary nematode exploration CURE (GENE-CURE). Using a combination of bioinformatic and molecular genetic tools, students performed structure-function analysis of disease-associated variants of uncertain significance (VUS) in human orthologs. With the aid of a series of workshop-style research sessions, students worked in teams of two to six members to identify a conserved VUS locus across species and design and test a polymerase chain reaction-based assay for targeted editing of a gene in the nematode and downstream genotyping. Research session discussions, responsible conduct of research training, electronic laboratory notebook, project reports, quizzes, and group poster presentations at a research symposium were assessed for mastery of learning objectives and research progress. Self-reflections were collected from students to assess engagement, science identity, and science efficacy. Qualitative analysis of these reflections indicated several gains suggesting that all students found many aspects of the GENE-CURE rewarding (learning process of research, self-confidence in research and science identity, and personal interest) and challenging (iterative research and failure, time management, COVID-19 pandemic, and life issues).","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"24 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10720527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a faculty support program for fostering enriching undergraduate laboratory experiences under limited resource conditions. 在资源有限的条件下，制定教师支持计划，丰富本科生的实验室体验。

IF 1.9

Journal of Microbiology & Biology Education Pub Date : 2023-11-15 eCollection Date: 2023-12-01 DOI: 10.1128/jmbe.00141-23

Anuttama Kulkarni

{"title":"Developing a faculty support program for fostering enriching undergraduate laboratory experiences under limited resource conditions.","authors":"Anuttama Kulkarni","doi":"10.1128/jmbe.00141-23","DOIUrl":"https://doi.org/10.1128/jmbe.00141-23","url":null,"abstract":"Meaningful pedagogical reform requires good faculty training and support programs. Such support is particularly valuable when colleges and universities are trying to bring research and inquiry into the laboratory curricula under resource-limited conditions. In this situation, it may help to extend the scope of the faculty support program to include training for practicing experimental techniques, sustainable networking opportunities, and a space to learn about pedagogical reforms. From this perspective, we share our experience about building a faculty development program for public college teachers who teach undergraduate biology in India. Though we designed the program for low-resource settings, the experiments curated could very well represent core biological concepts typically identified by the international community. The activities and overall design of the program can be useful for initiating pedagogical reform in any college/university where the traditional approach to biology laboratory instruction predominates, and high-end research is not easy to access.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"24 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10720460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pete and the Missing Scissors: a primary literature-focused case study that highlights the impact of SARS-CoV-2 on splicing. 皮特和失踪的剪刀：以原始文献为重点的案例研究，突出了 SARS-CoV-2 对剪接的影响。

IF 1.9

Journal of Microbiology & Biology Education Pub Date : 2023-11-15 eCollection Date: 2023-12-01 DOI: 10.1128/jmbe.00123-23

Meagan Harrington, April Lane, Caroline Yencha, Kiran Kelly-Rajan, Laura E Ott

{"title":"Pete and the Missing Scissors: a primary literature-focused case study that highlights the impact of SARS-CoV-2 on splicing.","authors":"Meagan Harrington, April Lane, Caroline Yencha, Kiran Kelly-Rajan, Laura E Ott","doi":"10.1128/jmbe.00123-23","DOIUrl":"https://doi.org/10.1128/jmbe.00123-23","url":null,"abstract":"This case study was designed to help students explore the molecular mechanisms of the spliceosome and how SARS-CoV-2 impacts host cell spliceosomal function while interpreting figures from primary literature (A. K. Banjeree, et al., Cell 183:1325-1339, e1-e10, 2020, https://doi.org/10.1016/j.cell.2020.10.004). \"Pete and the Missing Scissors\" was designed and implemented in the spring of 2022 and fall of 2022 in two large-enrollment (150+) introductory molecular biology courses at a large, public research institution. The case study was formatted in alignment with the National Center for Case Study Teaching in Science (NCCSTS) framework, which has been shown to be an effective, student-centered approach to teaching complex biological concepts at the undergraduate level. The case study had four student learning objectives (SLOs) that aligned with Bloom's Revised Taxonomy and required students to develop an understanding of the molecular mechanisms of splicing and analyze and interpret a figure from primary literature. Both formative and summative assessment questions are included in this activity, with each question mapping to one of the case study SLOs. Summative assessment questions were given in a pre-/post-manner, and a paired t-test was used to evaluate differences between students' pre- and post-assessment scores. Assessment results demonstrated that students in both courses mastered each of the SLOs of this case study, given the significant increase in post-assessment scores compared to the pre-assessment. These findings indicate that the \"Pete and the Missing Scissors\" case study is an effective approach to develop students' understanding of the spliceosome, as well as ability to interpret figures from primary literature.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"24 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10720516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chatbot responses suggest that hypothetical biology questions are harder than realistic ones 聊天机器人的回答表明，假设的生物学问题比现实问题更难

Journal of Microbiology & Biology Education Pub Date : 2023-11-07 DOI: 10.1128/jmbe.00153-23

Gregory J. Crowther, Usha Sankar, Leena S. Knight, Deborah L. Myers, Kevin T. Patton, Lekelia D. Jenkins, Thomas A. Knight

{"title":"Chatbot responses suggest that hypothetical biology questions are harder than realistic ones","authors":"Gregory J. Crowther, Usha Sankar, Leena S. Knight, Deborah L. Myers, Kevin T. Patton, Lekelia D. Jenkins, Thomas A. Knight","doi":"10.1128/jmbe.00153-23","DOIUrl":"https://doi.org/10.1128/jmbe.00153-23","url":null,"abstract":"ABSTRACT The biology education literature includes compelling assertions that unfamiliar problems are especially useful for revealing students’ true understanding of biology. However, there is only limited evidence that such novel problems have different cognitive requirements than more familiar problems. Here, we sought additional evidence by using chatbots based on large language models as models of biology students. For human physiology and cell biology, we developed sets of realistic and hypothetical problems matched to the same lesson learning objectives (LLOs). Problems were considered hypothetical if (i) known biological entities (molecules and organs) were given atypical or counterfactual properties (redefinition) or (ii) fictitious biological entities were introduced (invention). Several chatbots scored significantly worse on hypothetical problems than on realistic problems, with scores declining by an average of 13%. Among hypothetical questions, redefinition questions appeared especially difficult, with many chatbots scoring as if guessing randomly. These results suggest that, for a given LLO, hypothetical problems may have different cognitive demands than realistic problems and may more accurately reveal students’ ability to apply biology core concepts to diverse contexts. The Test Question Templates (TQT) framework, which explicitly connects LLOs with examples of assessment questions, can help educators generate problems that are challenging (due to their novelty), yet fair (due to their alignment with pre-specified LLOs). Finally, ChatGPT’s rapid improvement toward expert-level answers suggests that future educators cannot reasonably expect to ignore or outwit chatbots but must do what we can to make assessments fair and equitable.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"1 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135479636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance education under One Health umbrella 在同一个健康保护伞下开展抗微生物药物耐药性教育

Journal of Microbiology & Biology Education Pub Date : 2023-10-30 DOI: 10.1128/jmbe.00148-23

Daniel M. Czyz, Sarah Al-Mazroa Smith

引用次数: 0

Use of a microgravity analog to explore the effects of simulated microgravity on the development of Escherichia coli K12 biofilms 利用微重力模拟探讨模拟微重力对大肠杆菌K12生物膜发育的影响

Journal of Microbiology & Biology Education Pub Date : 2023-10-27 DOI: 10.1128/jmbe.00062-23

Janelle Hicks, Collin Topolski, Alba A. Chavez, Hugo A. Castillo

引用次数: 0

Community involvement in addressing the antibiotic crisis 社区参与解决抗生素危机

Journal of Microbiology & Biology Education Pub Date : 2023-10-25 DOI: 10.1128/jmbe.00136-23

Angelo Kolokithas, Brian Merkel, David Hunnicutt, Lucy Fenzl, Matthew Petersen

{"title":"Community involvement in addressing the antibiotic crisis","authors":"Angelo Kolokithas, Brian Merkel, David Hunnicutt, Lucy Fenzl, Matthew Petersen","doi":"10.1128/jmbe.00136-23","DOIUrl":"https://doi.org/10.1128/jmbe.00136-23","url":null,"abstract":"ABSTRACT Increasing student interest and success in STEM education is a top priority for many postsecondary educational institutions. One well-documented approach to both priorities is to have students participate in a Course Undergraduate Research Experience (CURE). Faculty from several technical colleges and universities in Wisconsin teamed up with the Tiny Earth organization to offer a CURE to address the search for new antibiotics. Students enrolled in undergraduate microbiology courses engaged in research and participated in community outreach. To involve the community, faculty from various institutions joined an NFL team, the Green Bay Packers, and created the Tiny Earth in Titletown symposium. Here, students presented their work via scientific posters, to community and industry members, and networked with other scientists from around the region. The Tiny Earth in Titletown symposium started in 2018, was held again in 2019, and returned in 2022 following a 2-year hiatus due to the COVID-19 pandemic. Record attendance in 2022 suggests that community outreach and education may be helping restore trust in science that was lost during the pandemic.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"197 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135169071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing scientific literacy with a cyclic independent study assisted CURE detecting SARS-CoV-2 in wastewater 通过循环独立研究培养科学素养，协助CURE检测废水中的SARS-CoV-2

Journal of Microbiology & Biology Education Pub Date : 2023-10-25 DOI: 10.1128/jmbe.00147-23

Kristine Dye

{"title":"Developing scientific literacy with a cyclic independent study assisted CURE detecting SARS-CoV-2 in wastewater","authors":"Kristine Dye","doi":"10.1128/jmbe.00147-23","DOIUrl":"https://doi.org/10.1128/jmbe.00147-23","url":null,"abstract":"ABSTRACT The COVID-19 pandemic has exposed a high level of scientific illiteracy and mistrust that pervades the scientific and medical communities. This finding has proven the necessity of updating current methods used to expose undergraduates to research. The research in traditional course-based undergraduate research experiences (CUREs) is limited by undergraduate time constraints, skill level, and course structure, and consequently it does not attain the learning objectives or the high-impact, relevant studies achieved in graduate-level laboratories using a cyclic trainee/trainer model. Although undergraduate independent study (ISY) research more closely matches the structure and learning objectives of graduate-level research, they are uncommon as professors and universities typically view them as a significant time and resource burden with limited return. Cyclic independent study-assisted CUREs (CIS-CUREs) combine many positive aspects of ISY graduate-level research, and CUREs by pre-training ISY research lead to facilitate CURE proposal and project semesters in a cyclic model. The CIS-CURE approach allowed undergraduate students at Stetson University to perform and disseminate more rigorous, involved, long-term, and challenging research projects, such as the surveillance of SARS-CoV-2 in wastewater. In doing so, all students would have the opportunity to participate in a high-impact research project and consequently gain a more comprehensive training, reach higher levels of research dissemination, and increase their competitiveness after graduating. Together, CIS-CUREs generate graduates with higher scientific literacy and thus combat scientific mistrust in communities.","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135216528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0