KEIO JOURNAL OF MEDICINE最新文献_第8页

Identification of B.1.346 lineage of SARS-CoV-2 in Japan: Genomic evidence of re-entry of Clade 20C 日本严重急性呼吸系统综合征冠状病毒2型B.1.346谱系的鉴定：分支20C重新进入的基因组证据

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2021-02-01 DOI: 10.1101/2021.01.29.21250798

K. Abe, T. Shimura, T. Takenouchi, Yuka W. Iwasaki, H. Ishizu, Y. Uwamino, S. Uno, Jun Gotoh, N. Tachikawa, Y. Takeuchi, Junpei Katayama, H. Nozaki, Susumu Fujii, S. Seki, Morio Nakamura, Kazuhiro Uda, T. Misumi, J. Ishihara, Kenichiro Yamada, Toshio Kanai, S. Murai, Kazuhiro Araki, T. Ebihara, Haruhiko Shiomi, N. Hasegawa, Y. Kitagawa, M. Amagai, M. Suematsu, K. Kosaki

{"title":"Identification of B.1.346 lineage of SARS-CoV-2 in Japan: Genomic evidence of re-entry of Clade 20C","authors":"K. Abe, T. Shimura, T. Takenouchi, Yuka W. Iwasaki, H. Ishizu, Y. Uwamino, S. Uno, Jun Gotoh, N. Tachikawa, Y. Takeuchi, Junpei Katayama, H. Nozaki, Susumu Fujii, S. Seki, Morio Nakamura, Kazuhiro Uda, T. Misumi, J. Ishihara, Kenichiro Yamada, Toshio Kanai, S. Murai, Kazuhiro Araki, T. Ebihara, Haruhiko Shiomi, N. Hasegawa, Y. Kitagawa, M. Amagai, M. Suematsu, K. Kosaki","doi":"10.1101/2021.01.29.21250798","DOIUrl":"https://doi.org/10.1101/2021.01.29.21250798","url":null,"abstract":"Objectives Whole SARS-CoV-2 genome sequencing from COVID-19 patients is useful for infection control and regional trends evaluation. We report a lineage data collected from hospitals in the Kanto region of Japan. Methods We performed whole genome sequencing in specimens of 198 COVID-19 patients at 13 collaborating hospitals in the Kanto region. Phylogenetic analysis and fingerprinting of the nucleotide substitutions underwent to differentiate and classify the viral lineages. Results More than 90% of the strains belonged to Clade 20B and two lineages (B.1.1.284 and B.1.1.214) have been detected predominantly in the Kanto region. However, one sample from a COVID-19 patient in November 2020, belonged to the B.1.346 lineage of Clade 20C, which has been prevalent in western United States. The patient had no history of overseas travel and no contact with anyone who had travelled abroad, suggesting that this strain appeared likely to have been imported from western United States, across the strict quarantine barrier. Conclusion B.1.1.284 and B.1.1.214 have been identified predominantly in the Kanto region and B.1.346 of clade 20C in one patient was probably imported from western United States. These results illustrate that a decentralized network of hospitals can be significantly advantageous for monitoring regional molecular epidemiologic trends.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44109120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Outbreak of Health Care-associated Novel Coronavirus (SARS-coV-2) COVID-19 infections in the Spring of 2020. 2020年春季爆发与卫生保健相关的新型冠状病毒(SARS-coV-2) COVID-19感染。

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KEIO JOURNAL OF MEDICINE Pub Date : 2021-01-01 DOI: 10.2302/kjm.kjm-covid19-01

Yuko Kitagawa

引用次数: 1

Hypertrophic Cardiomyopathy: Diverse Pathophysiology Revealed by Genetic Research, Toward Future Therapy. 肥厚性心肌病:基因研究揭示的多种病理生理学，走向未来的治疗。

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2020-12-25 Epub Date: 2020-03-28 DOI: 10.2302/kjm.2019-0012-OA

Takeharu Hayashi

{"title":"Hypertrophic Cardiomyopathy: Diverse Pathophysiology Revealed by Genetic Research, Toward Future Therapy.","authors":"Takeharu Hayashi","doi":"10.2302/kjm.2019-0012-OA","DOIUrl":"https://doi.org/10.2302/kjm.2019-0012-OA","url":null,"abstract":"Hypertrophic cardiomyopathy (HCM) is an intractable disease that causes heart failure mainly due to unexplained severe cardiac hypertrophy and diastolic dysfunction. HCM, which occurs in 0.2% of the general population, is the most common cause of sudden cardiac death in young people. HCM has been studied extensively using molecular genetic approaches. Genes encoding cardiac β-myosin heavy chain, cardiac myosin-binding protein C, and troponin complex, which were originally identified as causative genes, were subsequently reported to be frequently implicated in HCM. Indeed, HCM has been considered a disease of sarcomere gene mutations. However, fewer than half of patients with HCM have mutations in sarcomere genes. The others have been documented to have mutations in cardiac proteins in various other locations, including the Z disc, sarcoplasmic reticulum, plasma membrane, nucleus, and mitochondria. Next-generation sequencing makes it possible to detect mutations at high throughput, and it has become increasingly common to identify multiple cardiomyopathy-causing gene mutations in a single HCM patient. Elucidating how mutations in different genes contribute to the disease pathophysiology will be a challenge. In studies using animal models, sarcomere mutations generally tend to increase myocardial Ca2+ sensitivity, and some mutations increase the activity of myosin ATPase. Clinical trials of drugs to treat HCM are ongoing, and further new therapies based on pathophysiological analyses of the causative genes are eagerly anticipated.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":"69 4","pages":"77-87"},"PeriodicalIF":2.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2302/kjm.2019-0012-OA","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37782488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antifungal Agent Luliconazole Inhibits the Growth of Mouse Glioma-initiating Cells in Brain Explants. 抗真菌剂露立康唑抑制小鼠脑外植体胶质瘤起始细胞的生长。

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2020-12-25 Epub Date: 2020-07-31 DOI: 10.2302/kjm.2020-0001-OA

Hideaki Nagashima, Naoyoshi Koike, Kazunari Yoshida, Hideyuki Saya, Oltea Sampetrean

{"title":"Antifungal Agent Luliconazole Inhibits the Growth of Mouse Glioma-initiating Cells in Brain Explants.","authors":"Hideaki Nagashima, Naoyoshi Koike, Kazunari Yoshida, Hideyuki Saya, Oltea Sampetrean","doi":"10.2302/kjm.2020-0001-OA","DOIUrl":"https://doi.org/10.2302/kjm.2020-0001-OA","url":null,"abstract":"Imidazole antifungal compounds exert their antipathogenic effects through inhibition of sterol biosynthesis. These drugs have also recently been identified as candidate anticancer agents for several solid tumors including glioblastoma. However, their effects on glioma-initiating cells (GICs), i.e., glioma cells with stemlike properties that are able to initiate tumors, remain unclear. Consequently, we examined the effects of the optically active imidazole compound luliconazole on mouse GICs and GIC-based tumors. Luliconazole impaired in a concentration-dependent manner the growth of spheres formed by GICs in vitro. In contrast to the inhibitory effects of ionizing radiation and temozolomide on sphere growth, that of luliconazole was attenuated by the addition of exogenous cholesterol. Exposure to luliconazole of brain slices derived from mice with orthotopic GIC implants for 4 days in culture resulted in a marked increase in the number of tumor cells positive for cleaved caspase-3, but without a similar effect on normal cells. Furthermore, in brain slices, luliconazole inhibited the expansion of GIC-based tumors and the parenchymal infiltration of tumor cells. Our findings therefore indicate that luliconazole effectively targets GICs, thereby providing further support for the antitumorigenic effects of imidazole antifungal compounds.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":"69 4","pages":"97-104"},"PeriodicalIF":2.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38227292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Circulating microRNAs: Next-generation Cancer Detection. 循环microrna:下一代癌症检测。

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KEIO JOURNAL OF MEDICINE Pub Date : 2020-12-25 Epub Date: 2020-05-13 DOI: 10.2302/kjm.2019-0011-OA

Juntaro Matsuzaki, Takahiro Ochiya

引用次数: 8

Historical overview. 历史概述。

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2020-10-01 DOI: 10.3109/00016486509127779

T. Fujino, R. Calderhead

引用次数: 0

Body Motion and Rowing Performance: Association between Hip Angle and Rowing Performance: A Pilot Study. 身体运动和划船表现:臀部角度和划船表现之间的关系:一项初步研究。

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2020-09-25 Epub Date: 2020-01-23 DOI: 10.2302/kjm.2019-0007-OA

Masato Fumoto, Yasushi Sera, Koichiro Azuma, Kazuki Sato, Hideo Matsumoto

{"title":"Body Motion and Rowing Performance: Association between Hip Angle and Rowing Performance: A Pilot Study.","authors":"Masato Fumoto, Yasushi Sera, Koichiro Azuma, Kazuki Sato, Hideo Matsumoto","doi":"10.2302/kjm.2019-0007-OA","DOIUrl":"https://doi.org/10.2302/kjm.2019-0007-OA","url":null,"abstract":"The importance of aerobic fitness in rowing has been widely studied, and it is accepted that aerobic fitness is a key factor in rowing performance. In contrast, the impact of rowing efficacy, especially rowing form, on rowing performance has not yet been fully elucidated. The present study aimed to investigate this subject via the analysis of hip kinematics and the association of this variable with 2000 m ergometer rowing test performance. Eleven adult male rowers underwent a 2000 m rowing test on an ergometer and the exhaled gas was analyzed. The hip joint angle, the pelvic rotation, and the knee joint angle were measured at the catch position throughout the test. Peak VO2 was strongly associated with the time taken to complete the test (ρ=-0.96, P<0.01), thereby confirming the importance of aerobic capacity in rowing performance. The variance of the hip joint angle of each rower was associated with peak VO2, lean mass, and test time (ρ=-0.72, -0.84, and 0.66, respectively, all P<0.05). Greater knee flexion was accompanied by larger posterior rotation of the pelvis (ρ=0.74, P<0.05), and was negatively associated with hip flexion (ρ=-0.76, P<0.05). Although we cannot confirm whether the consistency of the hip joint angle actually leads to better rowing performance, our results suggest that there are associations between the consistency of the hip joint angle, aerobic capacity, lean mass, and the time taken to complete the 2000 m ergometer rowing test.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":"69 3","pages":"66-75"},"PeriodicalIF":2.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2302/kjm.2019-0007-OA","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37569846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Direct Cardiac Reprogramming for Cardiovascular Regeneration and Differentiation. 直接心脏重编程用于心血管再生和分化。

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KEIO JOURNAL OF MEDICINE Pub Date : 2020-09-25 Epub Date: 2020-01-09 DOI: 10.2302/kjm.2019-0008-OA

Taketaro Sadahiro, Masaki Ieda

{"title":"Direct Cardiac Reprogramming for Cardiovascular Regeneration and Differentiation.","authors":"Taketaro Sadahiro, Masaki Ieda","doi":"10.2302/kjm.2019-0008-OA","DOIUrl":"https://doi.org/10.2302/kjm.2019-0008-OA","url":null,"abstract":"Cardiovascular disease is the leading cause of death worldwide. Cardiomyocytes have limited regenerative capacity; consequently, regenerative therapies are in high demand. There are currently several potential strategies for heart regeneration, with one approach involving in situ generation of new cardiomyocytes from endogenous cell sources. Direct cardiac reprogramming has emerged as a novel therapeutic approach to regenerating the damaged heart by directly converting endogenous cardiac fibroblasts into cardiomyocyte-like cells. Following our first report of direct cardiac reprogramming, significant advances have elucidated the molecular mechanisms associated with cardiac reprogramming. These advances have also improved cardiac-reprogramming efficiency by enabling direct in vivo cardiac reprogramming. Moreover, progress has been made in cardiac reprogramming of human fibroblasts. Although basic research has supported substantial progress in this field, numerous challenges remain in terms of clinical application. Here, we review the current state of cardiac reprogramming as a new technology for understanding and treating cardiovascular diseases.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":"69 3","pages":"49-58"},"PeriodicalIF":2.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2302/kjm.2019-0008-OA","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37524244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Clinical Features of Febrile Urinary Tract Infection Caused by Extended-spectrum Beta-lactamase-producing Escherichia Coli in Children. 产广谱β -内酰胺酶大肠杆菌致儿童发热性尿路感染的临床特点

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KEIO JOURNAL OF MEDICINE Pub Date : 2020-06-25 DOI: 10.2302/kjm.2019-0005-OA

Takuma Ohnishi, Yoshinori Mishima, Shohei Takizawa, Kentaro Tsutsumi, Atsuko Amemiya, Nao Akiyama, Yukiko Kanna, S. Asato, Mizue Tomita, M. Ikemiyagi, Nobuaki Shikoro, Maki Nakazawa, Nobuyoshi Kurihara, I. Kamimaki

{"title":"Clinical Features of Febrile Urinary Tract Infection Caused by Extended-spectrum Beta-lactamase-producing Escherichia Coli in Children.","authors":"Takuma Ohnishi, Yoshinori Mishima, Shohei Takizawa, Kentaro Tsutsumi, Atsuko Amemiya, Nao Akiyama, Yukiko Kanna, S. Asato, Mizue Tomita, M. Ikemiyagi, Nobuaki Shikoro, Maki Nakazawa, Nobuyoshi Kurihara, I. Kamimaki","doi":"10.2302/kjm.2019-0005-OA","DOIUrl":"https://doi.org/10.2302/kjm.2019-0005-OA","url":null,"abstract":"The global prevalence of infections caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has been increasing. In children, ESBL-producing E. coli manifest mostly as febrile urinary tract infections (fUTIs). This study aimed to elucidate the clinical features of fUTI resulting from ESBL-producing E. coli in Japanese patients. The clinical features of children with E. coli-related fUTI were retrospectively examined. These children underwent treatment at the National Hospital Organization Saitama Hospital, Japan, between May 2010 and April 2018. Urine specimens were obtained by either bladder catheterization or the clean-catch method. All children having positive urine cultures (≥104 colony-forming unit/mL for catheter specimens and ≥105 colony forming unit/mL for clean-catch specimens) and a fever of ≥38°C were considered to have fUTI. During the study period, 171 patients were diagnosed with E. coli-related fUTI. Among these, 17 (9.9%) fUTI cases were caused by ESBL-producing E. coli. A significant difference was noted in the median age of the populations having ESBL-producing E. coli and non-ESBL-producing E. coli infections (2 and 5 months, respectively); other characteristics were not significantly different between the two patient groups. ESBL-producing E. coli infections markedly increased in our hospital between 2013 and 2018. In the present study, young age was the only risk factor for fUTI caused by ESBL-producing E. coli identified in Japanese children.","PeriodicalId":46245,"journal":{"name":"KEIO JOURNAL OF MEDICINE","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2020-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2302/kjm.2019-0005-OA","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46467692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Organ Fabrication Using Pigs as An in Vivo Bioreactor. 用猪作为体内生物反应器制备器官。

IF 2

KEIO JOURNAL OF MEDICINE Pub Date : 2020-06-25 DOI: 10.2302/kjm.2019-0006-OA

E. Kobayashi, S. Tohyama, K. Fukuda

引用次数: 5