Acta Biologica Cracoviensia Series Botanica最新文献

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ENDOPOLYPLOIDY PATTERNS IN ORGANS OF TRIFOLIUM SPECIES (FABACEAE) 三叶草(豆科)各器官的内多倍体模式
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2014-09-11 DOI: 10.2478/ABCSB-2014-0011
V. Kocová, V. Kolarčik, N. Straková, P. Mártonfi
{"title":"ENDOPOLYPLOIDY PATTERNS IN ORGANS OF TRIFOLIUM SPECIES (FABACEAE)","authors":"V. Kocová, V. Kolarčik, N. Straková, P. Mártonfi","doi":"10.2478/ABCSB-2014-0011","DOIUrl":"https://doi.org/10.2478/ABCSB-2014-0011","url":null,"abstract":"The pattern of endopolyploidy in the genus Trifolium was studied in mature organs of T. montanum and T. repens at reproductive stage, with comparative data for T. pratense, all from natural populations. Endopolyploidy in root, stem, petiole, leaf, inflorescence stalk, sepal, petal, stamen and carpel was detected by flow cytometry. 2C, 4C and 8C nuclei were found in organs of T. montanum and T. repens, and additionally 16C nuclei in organs of T. repens. The organs of T. montanum and T. repens differed in degree of endopolyploidy based on cycle values calculated from flow cytometry data; it was lowest in leaf and sepal in T. montanum and T. repens, and highest in T. montanum in petal and carpel and in T. repens in petiole and inflorescence stalk. These results are also seen in the two or more peaks of interphase nuclei in the flow cytometry histograms. There were significant correlations between the organs of T. pratense and T. repens as well as substantial differences between Trifolium species in the degree of endopolyploidy. T. pratense showed higher absolute endopolyploidy than T. montanum and T. repens. Principal component analysis showed that individuals of T. repens and T. montanum are more similar to each other than to individuals of T. pratense in degree of endopolyploidy. The observed variation between species might be explained by phylogenetic relationships and genome size differences.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2014-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2014-0011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
INVOLVEMENT OF THE IAA-REGULATED ACC OXIDASE GENE PNACO3 IN PHARBITIS NIL FLOWER INHIBITION iaa调控的acc氧化酶基因pnaco3参与无花性咽炎抑制
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2014-09-11 DOI: 10.2478/ABCSB-2014-0013
E. Wilmowicz, K. Frankowski, A. Kućko, J. Kęsy, J. Kopcewicz
{"title":"INVOLVEMENT OF THE IAA-REGULATED ACC OXIDASE GENE PNACO3 IN PHARBITIS NIL FLOWER INHIBITION","authors":"E. Wilmowicz, K. Frankowski, A. Kućko, J. Kęsy, J. Kopcewicz","doi":"10.2478/ABCSB-2014-0013","DOIUrl":"https://doi.org/10.2478/ABCSB-2014-0013","url":null,"abstract":"The study examined the influence of light and auxin on the transcription level of PnACO3, a gene involved in ethylene production, in relation to the inhibitory effect of ethylene on flower induction in the short-day plant Pharbitis nil (=Ipomoea nil). Exogenous auxin was shown to increase the level of PnACO3 mRNA, with the effect depending on the experimental conditions. Light did not affect the level of PnACO3 mRNA. Applying auxin to seedling cotyledons at the beginning of inductive night boosted PnACO3 transcriptional activity even threefold during the next few hours, supporting our previous suggestion that the inhibitory effect of auxin on P. nil flowering results from its stimulatory effect on ethylene production.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2014-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2014-0013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
TAXONOMIC STATUS OF GALEOBDOLON LUTEUM HUDS. (LAMIACEAE) FROM CLASSICAL TAXONOMY AND PHYLOGENETICS PERSPECTIVES 标题galeobdolon luteum huds的分类地位。(lamiaceae)的经典分类学和系统发育观点
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0016
K. Krawczyk, T. Korniak, J. Sawicki
{"title":"TAXONOMIC STATUS OF GALEOBDOLON LUTEUM HUDS. (LAMIACEAE) FROM CLASSICAL TAXONOMY AND PHYLOGENETICS PERSPECTIVES","authors":"K. Krawczyk, T. Korniak, J. Sawicki","doi":"10.2478/ABCSB-2013-0016","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0016","url":null,"abstract":"This paper is both a review and a study. It discusses the taxonomic status of Yellow Archangel (Galeobdolon luteum Huds.) from historical and contemporary perspectives, and gives a comprehensive list of synonyms for the discussed genera, species and lower taxonomic units, including their publication details. In the study it is postulated that G. luteum should be included in the genus Lamium. The hypothesis is verified by a comparative analysis between the representatives of the genera Galeobdolon and Lamium in four DNA regions: ITS, accD, rpoC1 and trnH-psbA. The analysis supported the determination of phylogenetic relationships among the studied taxa: G. luteum is not genetically distant enough from Lamium to be considered a separate genus, and integration of Galeobdolon and Lamium is legitimate.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69074493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
TYROSOL GLUCOSYLTRANSFERASE ACTIVITY AND SALIDROSIDE PRODUCTION IN NATURAL AND TRANSFORMED ROOT CULTURES OF RHODIOLA KIRILOWII (REGEL) REGEL ET MAXIMOWICZ 红景天天然和转化根培养中酪醇葡萄糖基转移酶活性和红景天苷的产生
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0025
M. Grech-Baran, K. Sykłowska-Baranek, J. Giebułtowicz, P. Wroczynski, A. Pietrosiuk
{"title":"TYROSOL GLUCOSYLTRANSFERASE ACTIVITY AND SALIDROSIDE PRODUCTION IN NATURAL AND TRANSFORMED ROOT CULTURES OF RHODIOLA KIRILOWII (REGEL) REGEL ET MAXIMOWICZ","authors":"M. Grech-Baran, K. Sykłowska-Baranek, J. Giebułtowicz, P. Wroczynski, A. Pietrosiuk","doi":"10.2478/ABCSB-2013-0025","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0025","url":null,"abstract":"The study examined tyrosol glucosyltransferase activity and the efficiency of salidroside production in natural and transformed root cultures of Rhodiola kirilowii (Regel) Regel et Maximowicz. Neither enzyme activity nor salidroside accumulation were detected in natural and transformed root cultures maintained in media without tyrosol. To induce TGase activity in biotransformation reactions, tyrosol was added to natural and transformed root cultures on the day of inoculation. The first peak of TGase activity (0.23 U/μg) was detected on day 9 in natural root culture, accompanied by the highest salidroside content (15.79 mg/g d.w.), but TGase activity was highest (0.27 U/μg) on day 15. In transformed root culture, day 18 showed the highest TGase activity (0.15 U/μg), which coincided with the highest salidroside content (2.4 mg/g d.w.). Based on these results, tyrosol was added to the medium on the days of highest previously detected activity of TGase: day 15 for natural root cultures and day 18 for transformed root cultures. This strategy gave significantly higher yields of salidroside than in the cultures supplemented with tyrosol on the day of inoculation. In natural root culture, salidroside production reached 21.89 mg/g d.w., while precursor feeding in transformed root cultures caused a significant increase in salidroside accumulation to 7.55 mg/g d.w. In all treatments, salidroside production was lower in transformed than in natural root cultures.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
EFFECT OF CAMP LEVEL ON THALLUS GROWTH AND GAMETANGIA DEVELOPMENT IN MACROALGA CHARA VULGARIS (CHARALES, CHAROPHYTA) camp水平对chara vulgaris (charales, charophyta)菌体生长和配子体发育的影响
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0029
A. Domanska, M. Godlewski, A. Kobylińska
{"title":"EFFECT OF CAMP LEVEL ON THALLUS GROWTH AND GAMETANGIA DEVELOPMENT IN MACROALGA CHARA VULGARIS (CHARALES, CHAROPHYTA)","authors":"A. Domanska, M. Godlewski, A. Kobylińska","doi":"10.2478/ABCSB-2013-0029","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0029","url":null,"abstract":"cAMP is a second messenger which plays a regulatory role in a wide variety of biological processes in organisms ranging from prokaryotes to higher eukaryotes, but knowledge of its role in macroalgae and vascular plants is limited. We modified cAMP levels in the macroalga Chara vulgaris thallus and studied the effects on thallus growth and gametangia development: db-cAMP (permeable analog of cAMP), adenylate cyclase (AC) activator, forskolin and theophylline (cAMP phosphodiesterase (PDE) inhibitor) were used to elevate cAMP levels, and the AC inhibitors 2'-dAdo and 2'-d3'-AMP were used to decrease them. The results suggest that in Chara vulgaris the cAMP pathway may regulate both vegetative thallus growth and gametangia development, and that these effects may depend on this second-messenger level. Elevated cAMP stimulated thallus growth and delayed gametangia development; decreased cAMP inhibited thallus growth and accelerated maturation of both antheridia and oogonia. These results suggest that the cAMP pathway participates in regulation of developmental processes in Chara vulgaris and that thallus growth and gametangia development require different cAMP lev- els in cells.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
EFFECT OF PEA APHID INFESTATION ON ACTIVITY OF AMINO ACID DECARBOXYLASES IN PEA TISSUES 豌豆蚜虫侵害对豌豆组织氨基酸脱羧酶活性的影响
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0021
C. Sempruch, W. Marczuk, B. Leszczyński, Agnieszka Kozak, W. Zawadzka, Agnieszka Klewek, J. Jankowska
{"title":"EFFECT OF PEA APHID INFESTATION ON ACTIVITY OF AMINO ACID DECARBOXYLASES IN PEA TISSUES","authors":"C. Sempruch, W. Marczuk, B. Leszczyński, Agnieszka Kozak, W. Zawadzka, Agnieszka Klewek, J. Jankowska","doi":"10.2478/ABCSB-2013-0021","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0021","url":null,"abstract":"The study examined changes in lysine decarboxylase (LDC), ornithine decarboxylase (ODC) and tyrosine decarboxylase (TyDC) activity in tissues of pea (Pisum sativum L.) infested by the pea aphid (Acyrthosiphon pisum Harris). The aphid induced increased ODC activity after one day and at two weeks. The effect was clearly systemic. TyDC activity increased after one day and at one week at feeding sites (aerial parts), while LDC activity increased only after one day of infestation and then decreased. Attack by aphids also affected enzyme activity in root tissues not directly damaged by the herbivores. The mechanisms of the response induced by pea aphid infestation in pea are discussed.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69074923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
ENCAPSULATION OF CENTAURIUM ERYTHRAEA RAFN – AN EFFICIENT METHOD FOR REGENERATION OF TRANSGENIC PLANTS 一种有效的转基因植株再生方法——赤马尾草包封
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0022
E. Piątczak, H. Wysokińska
{"title":"ENCAPSULATION OF CENTAURIUM ERYTHRAEA RAFN – AN EFFICIENT METHOD FOR REGENERATION OF TRANSGENIC PLANTS","authors":"E. Piątczak, H. Wysokińska","doi":"10.2478/ABCSB-2013-0022","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0022","url":null,"abstract":"An effective procedure for producing transformed Centaurium erythraea plants from synthetic seeds is described. Explants were encapsulated in 3% sodium alginate with 3% sucrose. Encapsulated hairy roots were cultured on half-strength Murashige and Skoog (1/2 MS) or Woody Plant (WPM) agar-solidified regeneration media supplemented with 6-benzylaminopurine (BAP) or without the cytokinin. The use of WPM with 0.5 mg/L of BAP gave the best shoot formation frequency (86%) and mean number of shoots (15) per root segment. Shoots rooted with 97% frequency on 1/2 MS without growth regulators. Encapsulated shoot buds were cultured on onethird-strength MS agar medium (1/3 MS) supplemented with indole-3-butyric acid (IBA) (0.05 mg/L). The plantlet conversion frequency was 32%. The encapsulated hairy roots and shoot buds were stored for 4, 6 or 14 weeks at 4°C. Synthetic seeds encapsulated with 3% sodium alginate with 3% sucrose stored at 4°C remained viable for 6 weeks but their developmental parameters significantly decreased. Adding nutrient medium and growth regulator to the alginate matrix increased plantlet recovery from both non-stored and stored synthetic seeds: synthetic seeds retained their viability and ability to form plantlets even after 14 weeks of storage. Regenerated transformed plantlets of C. erythraea were acclimatized in the greenhouse.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
CHLOROPHYLL CONTENT OF APHID-INFESTED SEEDLING LEAVES OF FIFTEEN MAIZE GENOTYPES 15个玉米基因型蚜虫侵染幼苗叶片叶绿素含量
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0023
H. Sytykiewicz, P. Czerniewicz, I. Sprawka, R. Krzyżanowski
{"title":"CHLOROPHYLL CONTENT OF APHID-INFESTED SEEDLING LEAVES OF FIFTEEN MAIZE GENOTYPES","authors":"H. Sytykiewicz, P. Czerniewicz, I. Sprawka, R. Krzyżanowski","doi":"10.2478/ABCSB-2013-0023","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0023","url":null,"abstract":"We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than in S. avenae-infested plants. Chlorophyll depletion was greater after long-term (14 days) than after short-term aphid infestation in the investigated host systems. Seedlings of Zlota Karlowa and Tasty Sweet were more damaged by aphid feeding; Ambrozja and Plomyk plants were less damaged by aphid feeding.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
INTRASPECIFIC VARIABILITY OF SEED MORPHOLOGY IN CAPPARIS SPINOSA L. 荆芥种子形态的种内变异。
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0027
E. Saadaoui, J. J. Gómez, E. Cervantes
{"title":"INTRASPECIFIC VARIABILITY OF SEED MORPHOLOGY IN CAPPARIS SPINOSA L.","authors":"E. Saadaoui, J. J. Gómez, E. Cervantes","doi":"10.2478/ABCSB-2013-0027","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0027","url":null,"abstract":"Morphological description of seeds is a required step for analysis of biodiversity in natural populations and may give clues to adaptive strategies in species evolution. A cardioid is the curve described by a point of one circumference rolling around another circumference of equal radius. Models based on adjustment of seed shape with cardioid curves have been described for Arabidopsis thaliana and the model legumes Lotus japonicus and Medicago truncatula. In this work the model is applied to analyze seed morphology in populations of two subspecies of Capparis spinosa growing in Tunisia. Adjustment of seed images to cardioid curves, followed by statistical analysis of similarity in the complete images as well as in each of four quadrants, allows an accurate description of seed shape. The results show differences in morphology between subspecies. Seeds of subsp. rupestris present higher diversity of shape than seeds of subsp. spinosa. This may indicate primitiveness of C. subsp. rupestris seeds, associated with nonspecialization. The results are discussed in relation to the ecological strategies of both subspecies in their evolution.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
IN VITRO PLANT REGENERATION OF COFFEE SENNA (SENNA OCCIDENTALIS) FROM HYPOCOTYL-DERIVED CALLUS 咖啡番泻下胚轴愈伤组织离体植株再生研究
IF 1.3 4区 生物学
Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI: 10.2478/ABCSB-2013-0031
T. Isah, A. Mujib
{"title":"IN VITRO PLANT REGENERATION OF COFFEE SENNA (SENNA OCCIDENTALIS) FROM HYPOCOTYL-DERIVED CALLUS","authors":"T. Isah, A. Mujib","doi":"10.2478/ABCSB-2013-0031","DOIUrl":"https://doi.org/10.2478/ABCSB-2013-0031","url":null,"abstract":"An efficient system for plant regeneration of Senna occidentalis from hypocotyl-derived callus was developed. Callus was induced from leaf and hypocotyl explants on MS medium amended with 9.04 μM 2,4-D + 2.22 μM BAP and 10.74 μM NAA + 2.22 μM BAP. Medium browning due to leaching of compounds from callus was encountered and ameliorated through incorporation of 2.84 μM ascorbic acid. Leaf-derived callus showed no shoot induction ability, while hypocotyl-derived callus produced shoots in all cytokinin-amended treatments and also in combination with 2.68 μM NAA. For shoot formation, BAP-augmented treatments were better than medium with Kin added. Rhizogenesis was better on 1/2 MS basal medium with IBA than in the NAA and IAA treatments. Regenerated plants were acclimatized with 94% survival and showed similar morphology to field-grown plants.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2013-0031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69075702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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