咖啡番泻下胚轴愈伤组织离体植株再生研究

IF 0.5 4区生物学 Q4 PLANT SCIENCES

Acta Biologica Cracoviensia Series Botanica Pub Date : 2013-12-01 DOI:10.2478/ABCSB-2013-0031

T. Isah, A. Mujib

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引用次数: 13

摘要

建立了一套高效的西番泻下胚轴愈伤组织再生体系。叶片和下胚轴外植体在添加9.04 μM 2,4- d + 2.22 μM BAP和10.74 μM NAA + 2.22 μM BAP的MS培养基上诱导愈伤组织。通过加入2.84 μM的抗坏血酸，可以改善愈伤组织中化合物浸出造成的中度褐变。在所有细胞分裂素和2.68 μM NAA处理下，叶源愈伤组织均无诱导出芽能力，而下胚轴源愈伤组织均有诱导出芽的能力。在芽形成方面，bap增强处理优于添加Kin的培养基。IBA在1/ 2ms基础培养基上的生根效果优于NAA和IAA处理。再生植株的驯化成活率为94%，其形态与田间栽培植株相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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IN VITRO PLANT REGENERATION OF COFFEE SENNA (SENNA OCCIDENTALIS) FROM HYPOCOTYL-DERIVED CALLUS

An efficient system for plant regeneration of Senna occidentalis from hypocotyl-derived callus was developed. Callus was induced from leaf and hypocotyl explants on MS medium amended with 9.04 μM 2,4-D + 2.22 μM BAP and 10.74 μM NAA + 2.22 μM BAP. Medium browning due to leaching of compounds from callus was encountered and ameliorated through incorporation of 2.84 μM ascorbic acid. Leaf-derived callus showed no shoot induction ability, while hypocotyl-derived callus produced shoots in all cytokinin-amended treatments and also in combination with 2.68 μM NAA. For shoot formation, BAP-augmented treatments were better than medium with Kin added. Rhizogenesis was better on 1/2 MS basal medium with IBA than in the NAA and IAA treatments. Regenerated plants were acclimatized with 94% survival and showed similar morphology to field-grown plants.

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来源期刊

Acta Biologica Cracoviensia Series Botanica 生物-植物科学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ACTA BIOLOGICA CRACOVIENSIA Series Botanica is an English-language journal founded in 1958, devoted to plant anatomy and morphology, cytology, genetics, embryology, tissue culture, physiology, biochemistry, biosystematics, molecular phylogenetics and phylogeography, as well as phytochemistry. It is published twice a year.