Journal of Experimental Therapeutics and Oncology最新文献

{"title":"Cystic lymphangioma of the lesser omentum in a pregnant woman: a case report and review of the literature.","authors":"Ozhan Ozdemir,&nbsp;Mustafa Erkan Sarı,&nbsp;Cemal Resat Atalay,&nbsp;Yüksel Kurban,&nbsp;Vusale Asgarova,&nbsp;Devrim Tuba Unal","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Lymphangiomas are rare benign tumors which are generally seen in pediatric population and the etiopathogenesis has not yet been understood. They occasionally occur in the head and neck or axillary region with only 5% of them being located in the abdominal or mediastinal cavity. These tumors may be asymptomatic or may cause acute abdominal symptoms due to the location and extention. In the English literature, only 4 cases of lymphangioma were reported to have occurred in the pregnancy period. Herein, we report a case of cystic lymphangioma of the lesser omentum detected incidentally on the ultrasonogram of a 21 year-old, 26-week pregnant woman. The patient was followed up uneventfully during pregnancy. Caesarean section was performed due to transverse presentation of the fetus, and the tumor was completely resected during the same session. The patient is recurrence-free after 1 year of postoperative follow-up.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35414208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective analysis of tolerance and outcomes of cutaneous malignant melanoma in patients receiving adjuvant interferon-alpha 2B: a community oncology perspective.","authors":"Muhammad Zubair Afzal, Vani Pinnamaneni, K C Birendra, Alan T Davis, Tracy J Koehler, Nehal Lakhani","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>Interferon alpha 2B (IFN-α) therapy in malignant melanoma has improved relapse free survival and overall survival but is considerably toxic and lowers the overall quality of life (QoL) substantially. A significant number of patients do not complete the full duration (one year) of therapy.</p><p><strong>Objective: </strong>The aim of this study was to evaluate patients' ability to tolerate IFN-α therapy and to compare our results to reported data in the literature.</p><p><strong>Methods: </strong>We conducted a retrospective review of patients diagnosed with cutaneous malignant melanoma who received IFN therapy after surgical resection. Patients were divided into two groups: patient who completed therapy (CIT) and those who did not (incomplete therapy, IIT). Duration of therapy was calculated. Reason for discontinuation and experienced side effects were reported. Statistical significance was determined at p &amp;#60; 0.05.</p><p><strong>Results: </strong>A total of 64 patients were included in the review. There were 16 (25%) patients were able to complete therapy. The most common reasons for discontinuing IFN-α therapy was fatigue (81.3%), fever (40.6%), depression (28.1%) and nausea (18.8%). Patients in the CIT group were younger than those in the IIT group (47.4 ± 14.2 vs 57.8 ± 11.9 years, mean ± SD; p = 0.011). There also seemed to be an association that those with the presence of advanced disease may have been more likely to complete therapy (node positive disease at the time of diagnosis, p = 0.07).</p><p><strong>Limitations: </strong>It is a retrospective study and has to rely on physician notes for the subjective data. For the survival analyses, the median follow-up times for both of the groups were less than 3.5 years.</p><p><strong>Conclusions: </strong>Younger patients were more likely to complete therapy. There was a trend towards an association between more advanced disease and the completion of therapy. Most common causes of discontinuation of therapy were fatigue, fever, depression, and nausea.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35467701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute effects of low-level laser therapy (660 nm) on oxidative stress levels in diabetic rats with skin wounds.","authors":"Amanda Silveira Denadai,&nbsp;Ricardo Dutra Aydos,&nbsp;Iandara Schettert Silva,&nbsp;Larissa Olmedo,&nbsp;Bruno Mendonça de Senna Cardoso,&nbsp;Baldomero Antonio Kato da Silva,&nbsp;Paulo de Tarso Camillo de Carvalho","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Laser therapy influences oxidative stress parameters such as the activity of antioxidant enzymes and the production of reactive oxygen species.</p><p><strong>Objective: </strong>To analyze the effects of low-level laser therapy on oxidative stress in diabetics rats with skin wounds.</p><p><strong>Methods: </strong>Thirty-six animals were divided into 4 groups: NDNI: non-diabetic rats with cutaneous wounds that not received laser therapy; NDI: non-diabetic rats with cutaneous wounds that received laser therapy; DNI: diabetic rats with skin wounds who did not undergo laser therapy; DI: rats with diabetes insipidus and cutaneous wounds and received laser therapy. The animals were treated with LLLT (660 nm, 100 mW, 6 J/cm, spot size 0.028 cm). On the day of killing the animals, tissue-wrapped cutaneous wounds were collected and immediately frozen, centrifuged, and stored to analyze malondialdehyde (MDA) levels.</p><p><strong>Results: </strong>Significant difference was observed within the groups of MDA levels (ANOVA, p = 0.0001). Tukey's post-hoc test showed significantly lower values of MDA in irradiated tissues, both in diabetic and non-diabetic rats. ANOVA of the diabetic group revealed a significant difference (p &amp;#60; 0.01) when all groups, except NDI and DI, were compared.</p><p><strong>Conclusions: </strong>LLLT was effective in decreasing MDA levels in acute surgical wounds in diabetic rats.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35467700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population-specific genetic variation at microRNA-629-binding site in the 3'-UTR of NBS1 gene in prostate cancer patients.","authors":"Ammad Ahmad Farooqi,&nbsp;Syed M Kamran Majeed,&nbsp;Qaisar Mansoor,&nbsp;Muhammad Ismail","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Prostate cancer is a genomically complex disease and recently emerging scientific evidence is adding new pieces of information into existing pool of knowledge of oncology. Prostate cancer cells tactfully rewire signaling cascades in presence or absence of androgen. We do not have finer proteome and genome based patient related information of our cancer patients.</p><p><strong>Methodology: </strong>In the present laboratory research, we studied 3' UTR C/T and A/G polymorphism in NBS1 gene in 100 prostate cancer patients and 100 healthy individuals without any previous clinical history, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.</p><p><strong>Results: </strong>For rs13312986 A&amp;#62;G genotypes, AA was 78% in patients and 80% in controls. AG was 21% in patients and 20% in controls. GG was 1% in patients and none was detected in control. Allelic frequencies for A was 0.885% in patients and 0.9% in controls. Allelic frequencies for G were 0.115% in patients and 0.1% in controls. For rs14448 T&amp;#62;C genotypes, TC was 23% in patients and 20% in controls. TT was 77% in patients and 80% in controls. CC was not detected either in patients or controls. T allele was 0.885% in patients and 0.9% in controls. C allele was 0.115% in patients and 0.1%-+ in controls.</p><p><strong>Conclusions: </strong>Future studies must converge on deeper and detailed mechanisms, as miRNA regulation of NBS1 is incompletely studied and exploration of these connections constitutes one of the most exciting areas in clinical oncology.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35414209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffuse large B cell lymphoma of hard palate: a case report with literature review.","authors":"Bhavana Rai,&nbsp;Madhusudan S Astekar,&nbsp;Bhari Sharanesha Manjunatha,&nbsp;Gaurav Sapra","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Lymphomas are diverse group of neoplasm affecting the lymphoreticular system. Diffuse large B cell lymphoma is the most frequently diagnosed type of non-Hodgkin lymphoma and is the fifth most frequent malignancy, accounting for about 40% of cases reported. In the present case report a 40 year old female patient complains of swelling on right side of face since 45 days and gradually increased to present size. Intra-orally an ulcero-proliferative growth was present on the right side of hard palate. Incisional biopsy was suggestive of Lymphoproliferative disorder. Panel of immunohistochemical antibodies were used; which showed positivity for CD45, PAX5, Bcl2, Ki67, CD138 and negativity for CD3, CD10, CD20 and CD30. Therefore through routine standard hematoxylin and eosin staining a clear cut diagnosis of specific lymphoma was difficult to obtain and thus immunohistochemistry plays an important role in confirming the pinpoint diagnosis.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35414332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An incidental finding of chronic salpingitis complications: Tubo-uterine fistula.","authors":"Aytekin Tokmak,&nbsp;Alev Esercan,&nbsp;Esma Sarıkaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Pelvic inflammatory disease (PID) is an infection of the upper genital tract in women that can include endometritis, parametritis, salpingitis, oophoritis, tubo-ovarian abscess, and peritonitis. The spectrum of the disease ranges from subclinical and asymptomatic infection to severe, lifethreatening illness; squealae include chronic pelvic pain, ectopic pregnancy, and infertility. In this case we report an uncommon complication of pelvic inflammatory disease, a tubo-uterian fistula. Our case was diagnosed by laparoscopy incidentally during assessment of infertility.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35467699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary lactate dehydrogenase as a prognostic marker in oral squamous cell carcinoma patients following surgical therapy.","authors":"Varsha Pathiyil,&nbsp;Audrey Madonna D'Cruz","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The objective of the present study was to assay the salivary levels of lactate dehydrogenase in patients with OSCC in comparison to healthy controls and to assess the changes in salivary levels of lactate dehydrogenase as prognostic markers of surgical therapy in oral squamous cell carcinoma patients. A case control study was undertaken comprising of 20 patients who were clinically diagnosed and histopathologically proven with oral squamous cell carcinoma and compared with 20 age and gender matched controls. Unstimulated whole saliva (2 ml) was collected by the spit method from every subject. The salivary LDH levels were analysed before surgery (baseline value) and compared with the control group. One month following surgical therapy, the salivary LDH levels were measured again and compared with the baseline values. The results obtained were subjected to statistical analysis using one way ANOVA and Pearson's correlation. The results of the present study showed that the mean salivary LDH levels in the study and control group were 457.06 ± 88.93 IU/L and 178.35 ± 120.54 IU/L respectively, the difference of which was statistically significant (p&amp;#60;0.001). Comparison of salivary LDH levels before surgery and 1 month after surgery showed that there was a decrease of salivary LDH levels, one month after surgical therapy, the difference of which was statistically significant (p&amp;#60;0.001). The study concluded that salivary lactate dehydrogenase levels could be used not only as a diagnostic marker but it could also be a valuable prognostic biomarker in patients treated surgically for OSCC.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35414771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of steroid and serum starvation on a human breast cancer adenocarcinoma cell line.","authors":"Maryam Nakhjavani,&nbsp;David J Stewart,&nbsp;Farshad H Shirazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Breast cancer is one of the most frequent cancers in women. Although there are many treatment protocols for this disease, there are still many patients who do not respond satisfactorily to the current treatment protocols. A better understanding of the nature of the tumor cells might provide a guide for better therapeutic procedures. In this study, the effect of metabolic stress was assessed on the human adenocarcinoma MCF-7 cell line. The cells were seeded in culture plates and after reaching log phase of their growth, cells were exposed to phenol red free-media supplemented with 10%, 0.5%, 0.25% and 0% charcoal-treated serum for 1 to 6 consecutive days. At 24-hour intervals, the cells' ability to proliferate (trypan blue assay), cells mitochondrial content (MTT assay) and intracellular protein content (SRB assay) was assessed. In each day of the study, the cells were released in complete media containing 10% serum for 24 hours and the effect of release was observed on cells. This study showed reduced basal growth rate and proliferation in response to harsh environmental conditions. Protein content of the cells also decreased. Mitochondrial content/activity of the cells however did not decrease, but instead a relative increase was observed at some time points. Releasing the cells caused increased cell proliferation in media with up to 0.5% serum, with an increase in intracellular protein content, although no significant mitochondrial function increase was observed. The results of this study confirm the adaptation of MCF-7 cells to environmental harsh conditions.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34968011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular glutathione level does not predict ovarian cancer cells' resistance after initial or repeated exposure to cisplatin.","authors":"Nastaran Nikounezhad,&nbsp;Maryam Nakhjavani,&nbsp;Farshad H Shirazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Cisplatin resistance development is a major obstacle in ovarian cancer treatment. One of the most important mechanisms underlying cisplatin resistance is drug detoxification by glutathione. In the present study, the importance of initial or repeated exposure to cisplatin in glutathione dependent resistance was investigated. To this purpose, some cisplatin sensitive and resistant variants of human ovarian cancer cell lines providing an appropriate range of cisplatin sensitivity were selected. Clonogenic survival assay was performed to evaluate cisplatin resistance and intracellular contents of reduced (GSH) and oxidized (GSSG) glutathione were analyzed using an HPLC method. Our results indicated that the intracellular GSH and GSSG concentrations were nearly equal in A2780 and A2780CP cells, while the A2780CP cells showed 14 times more resistance than the A2780 cells after initial exposure to cisplatin. A2780-R1 and A2780-R3 cells which have been repeatedly exposed to cisplatin also showed no significant difference in glutathione content, even though A2780-R3 was about two times more resistant than A2780-R1. Moreover, intracellular GSH/GSSG ratio decreased in the resistant cells, reflecting a shift towards a more oxidizing intracellular environment indicative of oxidative stress.</p><p><strong>Conclusion: </strong>As a conclusion, it seems that although the intracellular glutathione concentration increases after repeated exposure to cisplatin, there is no clear correlation between the intracellular GSH content in ovarian cancer cells and their resistance to cisplatin neither after initial nor after repeated exposure to this drug.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34968009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of odontogenic lesions among the Kanpur population: an institutional study.","authors":"Amrita Raj,&nbsp;Gayathri Ramesh,&nbsp;Ramesh Nagarajappa,&nbsp;Amit Pandey,&nbsp;Ankita Raj","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The frequency and incidence of odontogenic cysts and tumors are controversial and depends in the geographic location. These lesions have great clinical and histopathological diversity, and reflect a need for epidemiological profile to be considered when these lesions are studied.</p><p><strong>Aim: </strong>The purpose of this study was to determine the prevalence of odontogenic cysts and tumors over a period of 10 years and to compare with other data reported around the world.</p><p><strong>Materials and methods: </strong>Data for the study were obtained from the archives of the Department of Oral and Maxillofacial Pathology, diagnosed as the cases of Odontogenic cysts and tumors histopathologically, reported from January 2007 to March 2016. Case records of the patients that fit the histological classification of the World Health Organization (2005) were included in the study and the variables analyzed were: Age, sex, anatomical location, and histological type.</p><p><strong>Results: </strong>In a total of 1319 biopsies reported, 112 were odontogenic in nature. Of these 112 odontogenic lesions reported, Odontogenic Cysts accounted for 54.4% and odontogenic tumors of 45.53%. Of them 61.60% of the patients were males. Common age of occurrence was 21-50 years and mandibular posterior was the most common site of occurrence. Most common odontogenic cyst and tumor reported was dentigerous cyst and ameloblastoma respectively.</p><p><strong>Conclusion: </strong>This study provides epidemiological information on odontogenic cyst and tumors at an institutional level. The relative frequency of these cysts and tumors can be analyzed at a global level to understand their prevalence, incidence, biological behaviour, and distribution.</p>","PeriodicalId":45335,"journal":{"name":"Journal of Experimental Therapeutics and Oncology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34968013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}