Stem Cells and Cloning-Advances and Applications最新文献_第8页

From regenerative dentistry to regenerative medicine: progress, challenges, and potential applications of oral stem cells. 从再生牙科到再生医学:口腔干细胞的进展、挑战和潜在应用。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-12-04 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S51009

Li Xiao, Masanori Nasu

{"title":"From regenerative dentistry to regenerative medicine: progress, challenges, and potential applications of oral stem cells.","authors":"Li Xiao, Masanori Nasu","doi":"10.2147/SCCAA.S51009","DOIUrl":"https://doi.org/10.2147/SCCAA.S51009","url":null,"abstract":"Adult mesenchymal stem cells (MSCs) and epithelial stem cells play essential roles in tissue repair and self-healing. Oral MSCs and epithelial stem cells can be isolated from adult human oral tissues, for example, teeth, periodontal ligament, and gingiva. Cocultivated adult oral epithelial stem cells and MSCs could represent some developmental events, such as epithelial invagination and tubular structure formation, signifying their potentials for tissue regeneration. Oral epithelial stem cells have been used in regenerative medicine over 1 decade. They are able to form a stratified cell sheet under three-dimensional culture conditions. Both experimental and clinical data indicate that the cell sheets can not only safely and effectively reconstruct the damaged cornea in humans, but also repair esophageal ulcer in animal models. Oral MSCs include dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP), periodontal ligament stem cells (PDLSCs), and mesenchymal stem cells from gingiva (GMSCs). They are widely applied in both regenerative dentistry and medicine. DPSCs, SHED, and SCAP are able to form dentin-pulp complex when being transplanted into immunodeficient animals. They have been experimentally used for the regeneration of dental pulp, neuron, bone muscle and blood vessels in animal models and have shown promising results. PDLSCs and GMSCs are demonstrated to be ideal cell sources for repairing the damaged tissues of periodontal, muscle, and tendon. Despite the abovementioned applications of oral stem cells, only a few human clinical trials are now underway to use them for the treatment of certain diseases. Since clinical use is the end goal, their true regenerative power and safety need to be further examined. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"89-99"},"PeriodicalIF":2.9,"publicationDate":"2014-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S51009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32907219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 90

Specialized mouse embryonic stem cells for studying vascular development. 研究血管发育的特化小鼠胚胎干细胞。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-10-07 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S69554

Drew E Glaser, Andrew B Burns, Rachel Hatano, Magdalena Medrzycki, Yuhong Fan, Kara E McCloskey

{"title":"Specialized mouse embryonic stem cells for studying vascular development.","authors":"Drew E Glaser, Andrew B Burns, Rachel Hatano, Magdalena Medrzycki, Yuhong Fan, Kara E McCloskey","doi":"10.2147/SCCAA.S69554","DOIUrl":"https://doi.org/10.2147/SCCAA.S69554","url":null,"abstract":"Vascular progenitor cells are desirable in a variety of therapeutic strategies; however, the lineage commitment of endothelial and smooth muscle cell from a common progenitor is not well-understood. Here, we report the generation of the first dual reporter mouse embryonic stem cell (mESC) lines designed to facilitate the study of vascular endothelial and smooth muscle development in vitro. These mESC lines express green fluorescent protein (GFP) under the endothelial promoter, Tie-2, and Discomsoma sp. red fluorescent protein (RFP) under the promoter for alpha-smooth muscle actin (α-SMA). The lines were then characterized for morphology, marker expression, and pluripotency. The mESC colonies were found to exhibit dome-shaped morphology, alkaline phosphotase activity, as well as expression of Oct 3/4 and stage-specific embryonic antigen-1. The mESC colonies were also found to display normal karyotypes and are able to generate cells from all three germ layers, verifying pluripotency. Tissue staining confirmed the coexpression of VE (vascular endothelial)-cadherin with the Tie-2 GFP+ expression on endothelial structures and smooth muscle myosin heavy chain with the α-SMA RFP+ smooth muscle cells. Lastly, it was verified that the developing mESC do express Tie-2 GFP+ and α-SMA RFP+ cells during differentiation and that the GFP+ cells colocalize with the vascular-like structures surrounded by α-SMA-RFP cells. These dual reporter vascular-specific mESC permit visualization and cell tracking of individual endothelial and smooth muscle cells over time and in multiple dimensions, a powerful new tool for studying vascular development in real time. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"79-88"},"PeriodicalIF":2.9,"publicationDate":"2014-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S69554","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32758359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation. 猫羊膜多能细胞是非致瘤性的，用于细胞移植是安全的。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-08-27 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S67790

Atanasio S Vidane, Aline F Souza, Rafael V Sampaio, Fabiana F Bressan, Naira C Pieri, Daniele S Martins, Flavio V Meirelles, Maria A Miglino, Carlos E Ambrósio

{"title":"Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation.","authors":"Atanasio S Vidane, Aline F Souza, Rafael V Sampaio, Fabiana F Bressan, Naira C Pieri, Daniele S Martins, Flavio V Meirelles, Maria A Miglino, Carlos E Ambrósio","doi":"10.2147/SCCAA.S67790","DOIUrl":"https://doi.org/10.2147/SCCAA.S67790","url":null,"abstract":"Amnion-derived mesenchymal stem cells (AMSCs) are multipotent cells with an enhanced ability to differentiate into multiple lineages. AMSCs can be acquired through noninvasive methods, and therefore are exempt from the typical ethical issues surrounding stem cell use. The objective of this study was to isolate and characterize AMSCs from a cat amniotic membrane for future application in regenerative medicine. The cat AMSCs were harvested after mechanical and enzymatic digestion of amnion. In culture medium, the cat AMSCs adhered to a plastic culture dish and displayed a fibroblast-like morphology. Immunophenotyping assays were positive for the mesenchymal stem cell-specific markers CD73 and CD90 but not the hematopoietic markers CD34, CD45, and CD79. Under appropriate conditions, the cat AMSCs differentiated into osteogenic, chondrogenic, and adipogenic cell lineages. One advantage of cat AMSCs was nonteratogenicity, assessed 4 weeks post injection of undifferentiated AMSCs into immunodeficient mice. These findings suggest that cat amniotic membranes may be an important and useful source of mesenchymal stem cells for clinical applications, especially for cell or tissue replacement in chronic and degenerative diseases. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"71-8"},"PeriodicalIF":2.9,"publicationDate":"2014-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S67790","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32691575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Stem cell treatment for avascular necrosis of the femoral head: current perspectives. 干细胞治疗股骨头缺血性坏死:目前的观点。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-04-09 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S36584

Matthew T Houdek, Cody C Wyles, John R Martin, Rafael J Sierra

引用次数: 59

Amniotic membrane-derived stem cells: immunomodulatory properties and potential clinical application. 羊膜源性干细胞:免疫调节特性及潜在临床应用。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-03-24 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S58696

Carmen L Insausti, Miguel Blanquer, Ana M García-Hernández, Gregorio Castellanos, José M Moraleda

{"title":"Amniotic membrane-derived stem cells: immunomodulatory properties and potential clinical application.","authors":"Carmen L Insausti, Miguel Blanquer, Ana M García-Hernández, Gregorio Castellanos, José M Moraleda","doi":"10.2147/SCCAA.S58696","DOIUrl":"https://doi.org/10.2147/SCCAA.S58696","url":null,"abstract":"Epithelial and mesenchymal cells isolated from the amniotic membrane (AM) possess stem cell characteristics, differentiation potential toward lineages of different germ layers, and immunomodulatory properties. While their expansion and differentiation potential have been well studied and characterized, knowledge about their immunomodulatory properties and the mechanisms involved is still incomplete. These mechanisms have been evaluated on various target cells of the innate and the adaptive system and in animal models of different inflammatory diseases. Some results have evidenced that the immunomodulatory effect of AM-derived cells is dependent on cell-cell contact, but many of them have demonstrated that these properties are mediated through the secretion of suppressive molecules. In this review, we present an update on the described immunomodulatory properties of the derived amniotic cells and some of the proposed involved mechanisms. Furthermore, we describe some assays in animal models of different inflammatory diseases which reveal the potential use of these cells to treat such diseases. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"53-63"},"PeriodicalIF":2.9,"publicationDate":"2014-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S58696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32273607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 115

Adult human mesenchymal stromal cells and the treatment of graft versus host disease. 成人间充质间质细胞与移植物抗宿主病的治疗。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-02-28 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S37506

Richard P Herrmann, Marian J Sturm

引用次数: 29

Brain repair: cell therapy in stroke. 脑修复:中风中的细胞疗法。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-02-21 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S38003

Dheeraj Kalladka, Keith W Muir

{"title":"Brain repair: cell therapy in stroke.","authors":"Dheeraj Kalladka, Keith W Muir","doi":"10.2147/SCCAA.S38003","DOIUrl":"https://doi.org/10.2147/SCCAA.S38003","url":null,"abstract":"Stroke affects one in every six people worldwide, and is the leading cause of adult disability. Some spontaneous recovery is usual but of limited extent, and the mechanisms of late recovery are not completely understood. Endogenous neurogenesis in humans is thought to contribute to repair, but its extent is unknown. Exogenous cell therapy is promising as a means of augmenting brain repair, with evidence in animal stroke models of cell migration, survival, and differentiation, enhanced endogenous angiogenesis and neurogenesis, immunomodulation, and the secretion of trophic factors by stem cells from a variety of sources, but the potential mechanisms of action are incompletely understood. In the animal models of stroke, both mesenchymal stem cells (MSCs) and neural stem cells (NSCs) improve functional recovery, and MSCs reduce the infarct volume when administered acutely, but the heterogeneity in the choice of assessment scales, publication bias, and the possible confounding effects of immunosuppressants make the comparison of effects across cell types difficult. The use of adult-derived cells avoids the ethical issues around embryonic cells but may have more restricted differentiation potential. The use of autologous cells avoids rejection risk, but the sources are restricted, and culture expansion may be necessary, delaying treatment. Allogeneic cells offer controlled cell numbers and immediate availability, which may have advantages for acute treatment. Early clinical trials of both NSCs and MSCs are ongoing, and clinical safety data are emerging from limited numbers of selected patients. Ongoing research to identify prognostic imaging markers may help to improve patient selection, and the novel imaging techniques may identify biomarkers of recovery and the mechanism of action for cell therapies. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"31-44"},"PeriodicalIF":2.9,"publicationDate":"2014-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S38003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32173749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Direct reprogramming of adult cells: avoiding the pluripotent state. 成体细胞的直接重编程:避免多能状态。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-02-15 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S38006

Sophia Kelaini, Amy Cochrane, Andriana Margariti

引用次数: 85

Current perspectives in stem cell research for knee cartilage repair. 干细胞在膝关节软骨修复中的研究进展。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-01-16 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S42880

Patrick Orth, Ana Rey-Rico, Jagadeesh K Venkatesan, Henning Madry, Magali Cucchiarini

{"title":"Current perspectives in stem cell research for knee cartilage repair.","authors":"Patrick Orth, Ana Rey-Rico, Jagadeesh K Venkatesan, Henning Madry, Magali Cucchiarini","doi":"10.2147/SCCAA.S42880","DOIUrl":"https://doi.org/10.2147/SCCAA.S42880","url":null,"abstract":"Protocols based on the delivery of stem cells are currently applied in patients, showing encouraging results for the treatment of articular cartilage lesions (focal defects, osteoarthritis). Yet, restoration of a fully functional cartilage surface (native structural organization and mechanical functions) especially in the knee joint has not been reported to date, showing the need for improved designs of clinical trials. Various sources of progenitor cells are now available, originating from adult tissues but also from embryonic or reprogrammed tissues, most of which have already been evaluated for their chondrogenic potential in culture and for their reparative properties in vivo upon implantation in relevant animal models of cartilage lesions. Nevertheless, particular attention will be needed regarding their safe clinical use and their potential to form a cartilaginous repair tissue of proper quality and functionality in the patient. Possible improvements may reside in the use of biological supplements in accordance with regulations, while some challenges remain in establishing standardized, effective procedures in the clinics. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"1-17"},"PeriodicalIF":2.9,"publicationDate":"2014-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S42880","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32109348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 119

Patient-specific induced pluripotent stem cells in neurological disease modeling: the importance of nonhuman primate models. 神经系统疾病模型中的患者特异性诱导多能干细胞：非人灵长类动物模型的重要性。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2013-07-03 DOI: 10.2147/SCCAA.S34798

Zhifang Qiu, Steven L Farnsworth, Anuja Mishra, Peter J Hornsby

{"title":"Patient-specific induced pluripotent stem cells in neurological disease modeling: the importance of nonhuman primate models.","authors":"Zhifang Qiu, Steven L Farnsworth, Anuja Mishra, Peter J Hornsby","doi":"10.2147/SCCAA.S34798","DOIUrl":"10.2147/SCCAA.S34798","url":null,"abstract":"The development of the technology for derivation of induced pluripotent stem (iPS) cells from human patients and animal models has opened up new pathways to the better understanding of many human diseases, and has created new opportunities for therapeutic approaches. Here, we consider one important neurological disease, Parkinson's, the development of relevant neural cell lines for studying this disease, and the animal models that are available for testing the survival and function of the cells, following transplantation into the central nervous system. Rapid progress has been made recently in the application of protocols for neuroectoderm differentiation and neural patterning of pluripotent stem cells. These developments have resulted in the ability to produce large numbers of dopaminergic neurons with midbrain characteristics for further study. These cells have been shown to be functional in both rodent and nonhuman primate (NHP) models of Parkinson's disease. Patient-specific iPS cells and derived dopaminergic neurons have been developed, in particular from patients with genetic causes of Parkinson's disease. For complete modeling of the disease, it is proposed that the introduction of genetic changes into NHP iPS cells, followed by studying the phenotype of the genetic change in cells transplanted into the NHP as host animal, will yield new insights into disease processes not possible with rodent models alone. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"6 ","pages":"19-29"},"PeriodicalIF":2.9,"publicationDate":"2013-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32030129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0