Stem Cells and Cloning-Advances and Applications最新文献_第7页

Embryonic-maternal cross-talk via exosomes: potential implications. 胚胎-母体通过外泌体的串扰:潜在的影响。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-07-07 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S84991

Islam M Saadeldin, Hyun Ju Oh, Byeong Chun Lee

引用次数: 4

Cardiac tissue engineering and regeneration using cell-based therapy. 心脏组织工程与细胞再生。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-05-14 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S54204

Mohammad T Alrefai, Divya Murali, Arghya Paul, Khalid M Ridwan, John M Connell, Dominique Shum-Tim

引用次数: 4

Regulators of pluripotency and their implications in regenerative medicine. 多能性调控因子及其在再生医学中的意义。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-04-21 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S80157

Ahmed El-Badawy, Nagwa El-Badri

{"title":"Regulators of pluripotency and their implications in regenerative medicine.","authors":"Ahmed El-Badawy, Nagwa El-Badri","doi":"10.2147/SCCAA.S80157","DOIUrl":"https://doi.org/10.2147/SCCAA.S80157","url":null,"abstract":"The ultimate goal of regenerative medicine is to replace damaged tissues with new functioning ones. This can potentially be accomplished by stem cell transplantation. While stem cell transplantation for blood diseases has been increasingly successful, widespread application of stem cell therapy in the clinic has shown limited results. Despite successful efforts to refine existing methodologies and to develop better ones for reprogramming, clinical application of stem cell therapy suffers from issues related to the safety of the transplanted cells, as well as the low efficiency of reprogramming technology. Better understanding of the underlying mechanism(s) involved in pluripotency should accelerate the clinical application of stem cell transplantation for regenerative purposes. This review outlines the main decision-making factors involved in pluripotency, focusing on the role of microRNAs, epigenetic modification, signaling pathways, and toll-like receptors. Of special interest is the role of toll-like receptors in pluripotency, where emerging data indicate that the innate immune system plays a vital role in reprogramming. Based on these data, we propose that nongenetic mechanisms for reprogramming provide a novel and perhaps an essential strategy to accelerate application of regenerative medicine in the clinic. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"67-80"},"PeriodicalIF":2.9,"publicationDate":"2015-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S80157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33291727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Regenerative medicine in the treatment of idiopathic pulmonary fibrosis: current position. 再生医学在特发性肺纤维化治疗中的现状。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-04-15 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S49801

Diana Álvarez, Melanie Levine, Mauricio Rojas

{"title":"Regenerative medicine in the treatment of idiopathic pulmonary fibrosis: current position.","authors":"Diana Álvarez, Melanie Levine, Mauricio Rojas","doi":"10.2147/SCCAA.S49801","DOIUrl":"https://doi.org/10.2147/SCCAA.S49801","url":null,"abstract":"Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible disease of the lung that has no lasting option for therapy other than transplantation. It is characterized by replacement of the normal lung tissue by fibrotic scarring, honeycombing, and increased levels of myofibroblasts. The underlying causes of IPF are still largely unknown. The focus of the current review is the possible use of stem cell therapy, specifically mesenchymal stem cells (MSCs), a multipotent stromal cell population, which have demonstrated promising data in multiple animal models of pulmonary fibrosis (PF). The most studied source of MSCs is the bone marrow, although they can be found also in the adipose tissue and umbilical cord, as well as in the placenta. MSCs have immunomodulatory and tissue-protective properties that allow them to manipulate the local environment of the injured tissue, ameliorating the inflammation and promoting repair. Because IPF primarily affects older patients, the issue of aging is intrinsically linked to many aspects of the disease, including the age of the stem cells. Animal models have shown the success of MSC therapy in mitigating the fibrotic effects of bleomycin-induced PF. However, bleomycin, the most commonly used model for PF, is imperfect in mimicking IPF as it presents in humans, as the duration of the illness is not parallel or reversible, and honeycombing is not produced. Furthermore, the time of MSC dosage has proven to be critical in determining whether the cells will ultimately have a positive or negative effect on disease progression, since it has been demonstrated that the maximal beneficial effect of MSCs occurs during the early inflammatory phase of the disease and that there is no or negative effect during the late fibrotic phase. Therefore, all the current clinical trials of MSCs and IPF, though promising, should proceed with caution as we move toward true stem cell therapy for this disease. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"61-5"},"PeriodicalIF":2.9,"publicationDate":"2015-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S49801","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33263067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Postnatal oogenesis in humans: a review of recent findings. 人类产后卵子生成：最新研究成果综述。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-03-20 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S32650

Irma Virant-Klun

{"title":"Postnatal oogenesis in humans: a review of recent findings.","authors":"Irma Virant-Klun","doi":"10.2147/SCCAA.S32650","DOIUrl":"10.2147/SCCAA.S32650","url":null,"abstract":"In spite of generally accepted dogma that the total number of follicles and oocytes is established in human ovaries during the fetal period of life rather than forming de novo in adult ovaries, some new evidence in the field challenges this understanding. Several studies have shown that different populations of stem cells, such as germinal stem cells and small round stem cells with diameters of 2 to 4 μm, that resembled very small embryonic-like stem cells and expressed several genes related to primordial germ cells, pluripotency, and germinal lineage are present in adult human ovaries and originate in ovarian surface epithelium. These small stem cells were pushed into the germinal direction of development and formed primitive oocyte-like cells in vitro. Moreover, oocyte-like cells were also formed in vitro from embryonic stem cells and induced pluripotent stem cells. This indicates that postnatal oogenesis is not excluded. It is further supported by the occurrence of mesenchymal stem cells that can restore the function of sterilized ovaries and lead to the formation of new follicles and oocytes in animal models. Both oogenesis in vitro and transplantation of stem cell-derived \"oocytes\" into the ovarian niche to direct their natural maturation represent a big challenge for reproductive biomedicine in the treatment of female infertility in the future and needs to be explored and interpreted with caution, but it is still very important for clinical practice in the field of reproductive medicine. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"49-60"},"PeriodicalIF":2.9,"publicationDate":"2015-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/a1/sccaa-8-049.PMC4376261.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33194107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell technology for bone regeneration: current status and potential applications. 干细胞技术用于骨再生:现状和潜在应用。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-02-10 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S48423

Greg Asatrian, Dalton Pham, Winters R Hardy, Aaron W James, Bruno Peault

{"title":"Stem cell technology for bone regeneration: current status and potential applications.","authors":"Greg Asatrian, Dalton Pham, Winters R Hardy, Aaron W James, Bruno Peault","doi":"10.2147/SCCAA.S48423","DOIUrl":"https://doi.org/10.2147/SCCAA.S48423","url":null,"abstract":"Continued improvements in the understanding and application of mesenchymal stem cells (MSC) have revolutionized tissue engineering. This is particularly true within the field of skeletal regenerative medicine. However, much remains unknown regarding the native origins of MSC, the relative advantages of different MSC populations for bone regeneration, and even the biologic safety of such unpurified, grossly characterized cells. This review will first summarize the initial discovery of MSC, as well as the current and future applications of MSC in bone tissue engineering. Next, the relative advantages and disadvantages of MSC isolated from distinct tissue origins are debated, including the MSC from adipose, bone marrow, and dental pulp, among others. The perivascular origin of MSC is next discussed. Finally, we briefly comment on pluripotent stem cell populations and their possible application in bone tissue engineering. While continually expanding, the field of MSC-based bone tissue engineering and regeneration shows potential to become a clinical reality in the not-so-distant future. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"39-48"},"PeriodicalIF":2.9,"publicationDate":"2015-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S48423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33408281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Hedgehog signaling in cancer stem cells: a focus on hematological cancers. 癌症干细胞中的刺猬信号传导：聚焦血液癌症。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-01-16 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S58613

Victoria Campbell, Mhairi Copland

{"title":"Hedgehog signaling in cancer stem cells: a focus on hematological cancers.","authors":"Victoria Campbell, Mhairi Copland","doi":"10.2147/SCCAA.S58613","DOIUrl":"10.2147/SCCAA.S58613","url":null,"abstract":"The stem cell paradigm was first demonstrated in hematopoietic stem cells. Whilst classically it was cytokines and chemokines which were believed to control stem cell fate, more recently it has become apparent that the stem cell niche and highly conserved embryonic pathways play a key role in governing stem cell behavior. One of these pathways, the hedgehog signaling pathway, found in all organisms, is vitally important in embryogenesis, performing the function of patterning through early stages of development, and in adulthood, through the control of somatic stem cell numbers. In addition to these roles in health however, it has been found to be deregulated in a number of solid and hematological malignancies, components of the hedgehog pathway being associated with a poor prognosis. Further, these components represent viable therapeutic targets, with inhibition from a drug development perspective being readily achieved, making the hedgehog pathway an attractive potential therapeutic target. However, although the concept of cancer stem cells is well established, how these cells arise and the factors which influence their behavior are not yet fully understood. The role of the hedgehog signaling pathway and its potential as a therapeutic target in hematological malignancies is the focus of this review. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"27-38"},"PeriodicalIF":2.9,"publicationDate":"2015-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/f8/sccaa-8-027.PMC4325629.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33063298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Failure of Y-27632 to improve the culture of adult human adipose-derived stem cells. Y-27632不能改善成人脂肪来源干细胞的培养。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2015-01-07 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S66597

Nuno Jorge Lamas, Sofia C Serra, António J Salgado, Nuno Sousa

{"title":"Failure of Y-27632 to improve the culture of adult human adipose-derived stem cells.","authors":"Nuno Jorge Lamas, Sofia C Serra, António J Salgado, Nuno Sousa","doi":"10.2147/SCCAA.S66597","DOIUrl":"https://doi.org/10.2147/SCCAA.S66597","url":null,"abstract":"Y-27632 is a well-known inhibitor of the Rho-associated coiled kinase (ROCK) and has been shown to significantly improve the culture of a variety of multipotent stem cell types. However, the effects of Y-27632 on the expansion of adult human adipose-derived stem cell (hADSC) cultures remain to be established. Here, we aimed to characterize the effects of Y-27632 on the culture of hADSCs. Adult hADSCs were isolated from subjects submitted to elective plastic surgery procedures and cultivated in vitro under optimized conditions. Our results show that the continuous supplementation of hADSC cultures with Y-27632 led to decreased numbers of cells and decreased global metabolic viability of hADSC cultures when compared with control conditions. This effect appeared to be dependent on the continuous presence of the drug and was shown to be concentration-dependent and significant for 10 μM and 20 μM of Y-27632. Moreover, the Y-27632-induced decrease in hADSC numbers was not linked to a block in global cell proliferation, as cell numbers consistently increased from the moment of plating until passaging. In addition, Y-27632 was not able to increase the number of hADSCs present in culture 24 hours after passaging. Taken together, our results suggest that, in contrast to other stem cell types, Y-27632 supplementation is not a suitable strategy to enhance hADSC culture expansion. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"15-26"},"PeriodicalIF":2.9,"publicationDate":"2015-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S66597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32994164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Fetal exposure to a diabetic intrauterine environment resulted in a failure of cord blood endothelial progenitor cell adaptation against chronic hypoxia. 胎儿暴露于糖尿病宫内环境导致脐带血内皮祖细胞对慢性缺氧的适应失败。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-12-18 eCollection Date: 2015-01-01 DOI: 10.2147/SCCAA.S73658

U Deniz Dincer

{"title":"Fetal exposure to a diabetic intrauterine environment resulted in a failure of cord blood endothelial progenitor cell adaptation against chronic hypoxia.","authors":"U Deniz Dincer","doi":"10.2147/SCCAA.S73658","DOIUrl":"https://doi.org/10.2147/SCCAA.S73658","url":null,"abstract":"Gestational diabetes mellitus (GDM) has long-term health consequences, and fetal exposure to a diabetic intrauterine environment increases cardiovascular risk for her adult offspring. Some part of this could be related to their endothelial progenitor cells (EPCs). Understanding the vessel-forming ability of human umbilical cord blood (HUCB)-derived endothelial colony-forming cells (ECFCs) against pathological stress such as GDM response to hypoxia could generate new therapeutic strategies. This study aims to investigate the role of chronic hypoxia in EPCs functional and vessel-forming ability in GDM subjects. Each ECFC was expressed in endothelial and pro-angiogenic specific markers, namely endothelial nitric oxide synthase (eNOS), platelet (PECAM-1) endothelial cell adhesion molecule 1, vascular endothelial-cadherin CdH5 (Ca-dependent cell adhesion molecule), vascular endothelial growth factor A, (VEGFA) and insulin-like growth factor 1 (IGF1). Chronic hypoxia did not affect CdH5, but PECAM1 MRNA expressions were increased in control and GDM subjects. Control hypoxic and GDM normoxic VEGFA MRNA expressions and hypoxia-inducible factor 1-alpha (HIF1α) protein expressions were significantly increased in HUCB ECFCs. GDM resulted in most failure of HUCB ECFC adaptation and eNOS protein expressions against chronic hypoxia. Chronic hypoxia resulted in an overall decline in HUCB ECFCs' proliferative ability due to reduction of clonogenic capacity and diminished vessel formation. Furthermore, GDM also resulted in most failure of cord blood ECFC adaptation against chronic hypoxic environment. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"8 ","pages":"1-14"},"PeriodicalIF":2.9,"publicationDate":"2014-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S73658","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32956824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Some aspects of allogeneic stem cell transplantation in patients with myelodysplastic syndrome: advances and controversy. 异基因干细胞移植在骨髓增生异常综合征患者中的一些方面:进展和争议。

IF 2.9

Stem Cells and Cloning-Advances and Applications Pub Date : 2014-12-04 eCollection Date: 2014-01-01 DOI: 10.2147/SCCAA.S50514

Olga Blau, Igor Wolfgang Blau

{"title":"Some aspects of allogeneic stem cell transplantation in patients with myelodysplastic syndrome: advances and controversy.","authors":"Olga Blau, Igor Wolfgang Blau","doi":"10.2147/SCCAA.S50514","DOIUrl":"https://doi.org/10.2147/SCCAA.S50514","url":null,"abstract":"Myelodysplastic syndrome (MDS) is a heterogeneous group of myeloid disorders. MDS remains a disease of elderly patients; moreover, the incidence of high risk MDS is proportionally greater in elderly patients, with increased frequency of secondary acute myeloid leukemia, as well as adverse cytogenetic abnormalities. Allogeneic stem cell transplantation is a therapeutic approach with known curative potential for patients with MDS that allows the achievement of long-term disease control. Numerous controversies still exist regarding transplantation in MDS: timing of transplantation, disease status at transplantation and comorbidity, conditioning intensity, pretransplant therapy, and stem cell source. Various transplant modalities of different intensities and alternative donor sources are now in use. Current advances in transplant technology are allowing the consideration of older patients. This should result in a greater number of older patients benefiting from this potentially curative treatment modality. Despite advances in transplantation technology, there is still considerable morbidity and mortality associated with this approach. Nevertheless, with the introduction of reduced-intensity conditioning and thereby reduced early mortality, transplant numbers in MDS patients have significantly increased. Moreover, recent new developments with innovative drugs, including hypomethylating agents, have extended the therapeutic alternatives for MDS patients. Hypomethylating agents allow the delay of allogeneic stem cell transplantation by serving as an effective and well-tolerated means to reduce disease burden. ","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"7 ","pages":"101-8"},"PeriodicalIF":2.9,"publicationDate":"2014-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/SCCAA.S50514","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32908426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3