Stem Cells and Cloning-Advances and Applications最新文献

{"title":"Efficacy of Adipocyte-Derived Stem Cells-Conditioned Media in Telogen Effluvium.","authors":"Shadi Zari","doi":"10.2147/SCCAA.S432179","DOIUrl":"https://doi.org/10.2147/SCCAA.S432179","url":null,"abstract":"<p><strong>Objectives: </strong>To assess the effectiveness of adipocyte-derived mesenchymal stem cells-conditioned media (ADSC-CM) formulation in telogen effluvium patients.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted at a dermatology clinic in Jeddah, Saudi Arabia. The study included 50 consecutive patients aged 20-70 years, who were diagnosed with telogen effluvium. All patients received five monthly sessions of the same commercial ADSC-CM formulation, using a standardized application protocol. Pre- and post-intervention changes in trichometry parameters were analyzed.</p><p><strong>Results: </strong>There was a significant increase in mean hair density (up to 29.01 hair/cm²; effect size 0.7-1.0), cumulative hair thickness (up to 2.67 units; effect size 0.7-1.4), and the number of follicular hair units (up to 19.96%; effect size 1.0-1.3) in all scalp regions (p < 0.001), associated with a decrease in mean trichometry-derived Sinclair scale by 0.8-1.3 (p < 0.001). Positive outcomes were observed in 70%-92% of the patients depending on the parameter and scalp region. There was no impact of the patient's age on ADSC-CM efficacy.</p><p><strong>Conclusion: </strong>ADSC-CM was successfully applied as a new treatment option for patients with telogen effluvium. These findings provide another therapeutic and research area for dermatologists to optimize the management of telogen effluvium and reduce its impact on patients.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"77-89"},"PeriodicalIF":2.9,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Response to Article “Do Mesenchymal Stem Cells Influence Keloid Recurrence?” [Response to Letter] [Corrigendum]","authors":"Wanjala Ferdinand Nang'ole","doi":"10.2147/sccaa.s447846","DOIUrl":"https://doi.org/10.2147/sccaa.s447846","url":null,"abstract":"[This corrects the article DOI: 10.2147/SCCAA.S405738.].","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"38 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135614528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate Beads as a Promising Tool for Successful Production of Viable and Pluripotent Human-Induced Pluripotent Stem Cells in a 3D Culture System.","authors":"Sarah Alsobaie,&nbsp;Tamador Alsobaie,&nbsp;Amal F Alshammary,&nbsp;Manal Abudawood,&nbsp;Athanasios Mantalaris","doi":"10.2147/SCCAA.S409139","DOIUrl":"https://doi.org/10.2147/SCCAA.S409139","url":null,"abstract":"<p><strong>Purpose: </strong>Two-dimensional (2D)-based cell culture systems, limited by their inherent heterogeneity and scalability, are a bottleneck in the production of high-quality cells for downstream biomedical applications. Finding the optimal conditions for large-scale stem cell culture while maintaining good cellular status is challenging. The aim of this study was to assess the effects of three-dimensional (3D) culture on the viability, proliferation, self-renewal, and differentiation of human induced pluripotent stem cells (IPSCs).</p><p><strong>Patients and methods: </strong>Various culture conditions were evaluated to determine the optimal conditions to maintain the viability and proliferation of human IPSCs in a 3D environment: static versus dynamic culture, type of adhesion protein added to alginate (Matrigel™ versus gelatin), and the addition of Y-27632t on long-term 3D culture. The proliferation ability of the cells was evaluated via the MTS proliferation assay; the expression levels of the pluripotency markers Nanog and Oct3/4, PAX6 as an ectoderm marker, and laminin-5 and fibronectin as markers of extracellular matrix synthesis were assessed; and HIF1α and HIF2α levels were measured using quantitative reverse transcription polymerase chain reaction.</p><p><strong>Results: </strong>Using a high-aspect-ratio vessel bioreactor with a gentle, low-sheer, and low-turbulence environment with sufficient oxygenation and effective mass transfer of nutrients and waste, we verified its ability to promote cell proliferation and self-renewal. The findings showed that human IPSCs have the ability to maintain pluripotency in a feeder-free system and by inhibiting ROCK signaling and using hypoxia to improve single-cell viability in 3D culture. Furthermore, these cells demonstrated increased self-renewal and proliferation when inoculated as single cells in 3D alginate beads by adding RI during the culture period.</p><p><strong>Conclusion: </strong>Dynamic 3D culture is desirable for the large-scale expansion of undifferentiated human IPSCs.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"61-73"},"PeriodicalIF":2.9,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/76/4f/sccaa-16-61.PMC10544263.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41147459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Response to Article \"Do Mesenchymal Stem Cells Influence Keloid Recurrence?\" [Response to Letter].","authors":"Wanjala Ferdinand Nang'ole","doi":"10.2147/SCCAA.S405738","DOIUrl":"10.2147/SCCAA.S405738","url":null,"abstract":"","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"3-4"},"PeriodicalIF":2.9,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/5c/sccaa-16-3.PMC9899016.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10660547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria in Cancer Stem Cells: From an Innocent Bystander to a Central Player in Therapy Resistance.","authors":"Sireesha V Garimella,&nbsp;Siri Chandana Gampa,&nbsp;Pankaj Chaturvedi","doi":"10.2147/SCCAA.S417842","DOIUrl":"https://doi.org/10.2147/SCCAA.S417842","url":null,"abstract":"<p><p>Cancer continues to rank among the world's leading causes of mortality despite advancements in treatment. Cancer stem cells, which can self-renew, are present in low abundance and contribute significantly to tumor recurrence, tumorigenicity, and drug resistance to various therapies. The drug resistance observed in cancer stem cells is attributed to several factors, such as cellular quiescence, dormancy, elevated aldehyde dehydrogenase activity, apoptosis evasion mechanisms, high expression of drug efflux pumps, protective vascular niche, enhanced DNA damage response, scavenging of reactive oxygen species, hypoxic stability, and stemness-related signaling pathways. Multiple studies have shown that mitochondria play a pivotal role in conferring drug resistance to cancer stem cells, through mitochondrial biogenesis, metabolism, and dynamics. A better understanding of how mitochondria contribute to tumorigenesis, heterogeneity, and drug resistance could lead to the development of innovative cancer treatments.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"19-41"},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/00/sccaa-16-19.PMC10460581.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Response to Article \"Do Mesenchymal Stem Cells Influence Keloid Recurrence?\" [Letter].","authors":"Ariyani Noviantari,&nbsp;Frans Dany,&nbsp;Putri Reno Intan","doi":"10.2147/SCCAA.S403167","DOIUrl":"https://doi.org/10.2147/SCCAA.S403167","url":null,"abstract":"","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"1-2"},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/6a/sccaa-16-1.PMC9844817.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9227688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VEGF Expression in Umbilical Cord MSC Depends on the Patient's Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn - Preliminary Report.","authors":"Karolina Bieńko,&nbsp;Monika Leszcz,&nbsp;Marta Więckowska,&nbsp;Justyna Białek,&nbsp;Alicja Petniak,&nbsp;Rafał Szymanowski,&nbsp;Agnieszka Wilińska,&nbsp;Bartosz Piszcz,&nbsp;Arkadiusz Krzyżanowski,&nbsp;Anna Kwaśniewska,&nbsp;Bartosz J Płachno,&nbsp;Paulina Gil-Kulik,&nbsp;Janusz Kocki","doi":"10.2147/SCCAA.S399303","DOIUrl":"https://doi.org/10.2147/SCCAA.S399303","url":null,"abstract":"<p><strong>Introduction: </strong>Cells collected from Wharton's jelly are a rich source of mesenchymal stem cells. They can be easily obtained and grown using the adhesive method. They produce many types of proteins, including VEGF. Their role is to participate in angiogenesis, vasodilation, stimulation of cells to migrate, and chemotactic activity. The aim of this study was to evaluate expression of genes from the vascular endothelial growth factor family: <i>VEGFA, VEGFB</i> and <i>VEGFC</i> in MSC and the analysis of dependence of the expression of the studied genes on clinical factors related to the course of pregnancy and childbirth, and health of mother and child.</p><p><strong>Material and methods: </strong>The research material was an umbilical cord obtained from 40 patients hospitalized in the Department of Obstetrics and Pathology of Pregnancy of the Independent Public Clinical Hospital No.1 in Lublin. The age of the women was 21-46, all gave birth by cesarean section. Some of the patients suffered from hypertension and hypothyroidism. Material collected from patients immediately after delivery was subjected to enzymatic digestion with type I collagenase. The isolated cells were then cultured in adherent conditions, and then gene expression was assessed using qPCR and the immunophenotype of the cells was assessed cytometrically.</p><p><strong>Results: </strong>Conducted studies have shown significant differences in expression of VEGF family genes depending on clinical condition of mother and child. Significant differences in VEGF-family gene expression level in umbilical cord MSC collected from women with hypothyroidism, hypertension, time of labor and birth weight of the baby were shown.</p><p><strong>Conclusion: </strong>Probably due to hypoxia (caused, for example, by hypothyroidism or hypertension), the MSCs found in the umbilical cord may react with an increased expression of VEGF and a compensatory increase in the amount of secreted factor, the aim of which is, i.a., vasodilation and increase of blood supply to the fetus through the umbilical vessels.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"5-18"},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/08/sccaa-16-5.PMC10150760.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Regenerative Power of Stem Cells: Treating Bleomycin-Induced Lung Fibrosis.","authors":"Amrita Vats,&nbsp;Pankaj Chaturvedi","doi":"10.2147/SCCAA.S419474","DOIUrl":"https://doi.org/10.2147/SCCAA.S419474","url":null,"abstract":"<p><p>Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with no known cure, characterized by the formation of scar tissue in the lungs, leading to respiratory failure. Although the exact cause of IPF remains unclear, the condition is thought to result from a combination of genetic and environmental factors. One of the most widely used animal models to study IPF is the bleomycin-induced lung injury model in mice. In this model, the administration of the chemotherapeutic agent bleomycin causes pulmonary inflammation and fibrosis, which closely mimics the pathological features of human IPF. Numerous recent investigations have explored the functions of various categories of stem cells in the healing process of lung injury induced by bleomycin in mice, documenting the beneficial effects and challenges of this approach. Differentiation of stem cells into various cell types and their ability to modulate tissue microenvironment is an emerging aspect of the regenerative therapies. This review article aims to provide a comprehensive overview of the role of stem cells in repairing bleomycin-induced lung injury. It delves into the mechanisms through which various types of stem cells, including mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, and lung resident stem cells, exert their therapeutic effects in this specific model. We have also discussed the unique set of intermediate markers and signaling factors that can influence the proliferation and differentiation of alveolar epithelial cells both during lung repair and homeostasis. Finally, we highlight the challenges and opportunities associated with translating stem cell therapy to the clinic for IPF patients. The novelty and implications of this review extend beyond the understanding of the potential of stem cells in treating IPF to the broader field of regenerative medicine. We believe that the review paves the way for further advancements in stem cell therapies, offering hope for patients suffering from this debilitating and currently incurable disease.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":"16 ","pages":"43-59"},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/46/sccaa-16-43.PMC10505024.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10306736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-Derived Stem Cells (ASCs) for Regeneration of Intervertebral Disc Degeneration: Review Article.","authors":"F N U Romaniyanto,&nbsp;Ferdiansyah Mahyudin,&nbsp;Cita Rosita Sigit Prakoeswa,&nbsp;Hari Basuki Notobroto,&nbsp;Damayanti Tinduh,&nbsp;Ryan Ausrin,&nbsp;Fedik Abdul Rantam,&nbsp;Heri Suroto,&nbsp;Dwikora Novembri Utomo,&nbsp;Sholahuddin Rhatomy","doi":"10.2147/SCCAA.S379714","DOIUrl":"https://doi.org/10.2147/SCCAA.S379714","url":null,"abstract":"<p><p>The intervertebral disc (IVD) is an important structure in the human body because it functions as a weight-bearing. This structure undergoes a process of degeneration like the rest of the body and this process is known as intervertebral disc degeneration (IDD) which is the most common cause of low back pain (LBP). The current common management, either conservative or surgical, is pain-relieving and has not been able to restore degenerated disc optimally. Changes in the IVD microenvironment in IDD conditions make it difficult for the regeneration process to occur. Research to reverse the degeneration process continues to develop, one of them is the use of adipose-derived stem cells (ASCs). ASCs is superior due to the ability to differentiate into several other cells such as adipocytes, chondrocytes, and osteoblasts, it also has ability to act as immunomodulators by stimulating the migration of immune cells to damaged tissues. ASCs becomes a good choice because it is easy to obtain, low donor site morbidity, high proliferation rate, and excellent differentiation abilities. Research on the optimal preparation process for ASCs and their application to various disorders continues to advanced. This study aims to review the potential use of ASCs for regeneration of intervertebral disc degeneration.</p>","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":" ","pages":"67-76"},"PeriodicalIF":2.9,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/c0/sccaa-15-67.PMC9642326.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40692842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Response to Article \"Rho-Associated Protein Kinase Inhibitor and Hypoxia Synergistically Enhance the Self-Renewal, Survival Rate, and Proliferation of Human Stem Cells\" [Letter].","authors":"Frans Dany,&nbsp;Ratih Rinendyaputri","doi":"10.2147/SCCAA.S392521","DOIUrl":"https://doi.org/10.2147/SCCAA.S392521","url":null,"abstract":"We and the article by Alsobaie et al 1 with great interest, which studied the synergistic effect of rho-associated protein kinase (ROCK) inhibitor Y-27632 and hypoxic condition to maintain propagation of human induced-pluripotent stem cells (IPSCs). We would like to give our insights particularly on the characterization method of IPSCs in this study. First, we really appreciated the author’s effort by using pluripotency markers as examined by flow cytometry such as Oct-4, SSEA-1, TRA-1-81 and so forth to get an overview of IPSC stemness in their cell culture. However, it is also vital to check whether their potential of trilineage differentiation (ectodermal, mesodermal and endodermal) is still retained or not. This can be done through teratoma formation by injecting IPSC into NOD/SCID mice although this approach may encounter issues as these animals are prone to developing thymus tumors besides ethical aspect of animal sacrifice itself. 2,3 Alternatively, one may carry out embryoid body (EB) formation, an aggregate of pluripotent stem cells with three germ layers. EB offers a sustainable approximation of trilineage development and serves as early prediction of their tendency to differentiate into one of three embryonic tissues. 4 As in Supplementary an IPSC colony appeared to undergo spontaneous differentiation. This suggested that the utilization of pluripotency markers measured via flow cytometry is not enough to assert that IPSC stemness","PeriodicalId":44934,"journal":{"name":"Stem Cells and Cloning-Advances and Applications","volume":" ","pages":"65-66"},"PeriodicalIF":2.9,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/62/sccaa-15-65.PMC9618249.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40661453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}