A. Y. Shashko, U. Y. Bandarenka, M. Charnysh, D. Przhevalskaya, S. Usnich, Natalia L. Pshybytko, I. Smolich, V. Demidchik
{"title":"Modern phenotyping platforms and their application in plant biology and agriculture","authors":"A. Y. Shashko, U. Y. Bandarenka, M. Charnysh, D. Przhevalskaya, S. Usnich, Natalia L. Pshybytko, I. Smolich, V. Demidchik","doi":"10.33581/2521-1722-2020-2-15-25","DOIUrl":"https://doi.org/10.33581/2521-1722-2020-2-15-25","url":null,"abstract":"Phenotyping platforms is hardware and software systems that provide collection and processing of information about plant or other organism phenotypes. In the presented study, an analytical review of the phenotyping platforms of the world’s major manufacturers, their components, characteristics and applications in biology, agriculture and biotechnology was carried out. A comparison of the phenomics attitudes by key parameters was made, trends in the development of their sensory and program-analytical components were revealed. The assessment of the world market of phenotyping systems current state was made. The most important examples of phenomics facilities use for phenotyping in the field and laboratory conditions, fundamental and practical research in various fields of plant biology are given.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126006585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kristina V. Pristupa, Tatsiana A. Kukulianskaya, E. Khramtsova
{"title":"Analysis of the low-molecular weight antioxidants of transgenic plants Nicotiana tabacum under abiotic stress conditions","authors":"Kristina V. Pristupa, Tatsiana A. Kukulianskaya, E. Khramtsova","doi":"10.33581/2521-1722-2020-1-20-26","DOIUrl":"https://doi.org/10.33581/2521-1722-2020-1-20-26","url":null,"abstract":"We conducted a comparative analysis of biochemical parameters of non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown that the content of phenolic compounds (flavonoids), ascorbic acid, the total antioxidant activity of plants increased under abiotic stress.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125691225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Mackievic, S. Zvanarou, Artur A. Shyker, O. Turovets, Ihar I. Smolich, A. Sokolik, V. Demidchik
{"title":"Determination of NaCl- induced modifications in growth processes and induction of programmed cell death in sunflower roots","authors":"V. Mackievic, S. Zvanarou, Artur A. Shyker, O. Turovets, Ihar I. Smolich, A. Sokolik, V. Demidchik","doi":"10.33581/2521-1722-2019-3-13-20","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-13-20","url":null,"abstract":"Globally, about 30 % of all soils are damaged by salinity. Salinity leads to lower productivity of agro- and biocenosis, a decline in biodiversity and, as a result, significant economic losses. For the most important oil culture – sunflower (Helianthus annuus L.), primary reactions to salt stress are almost unexplored. This paper analyzes the impact of a wide range of NaCl concentrations on the growth of the main root of sunflower sprouts and the development of programmed cell death processes in trichoblasts. Experiments have shown that sunflower plants are relatively resistant to salinity (NaCl). They were able to grow at the level up to NaCl 250 mmol/L in the environment. Significant symptoms of programmed cell death were detected only at high concentrations of NaCl 250 –500 mmol/L. Previously undescribed in the literature, the reaction of reducing the number of symptoms of programmed cell death under150 mmol/L NaCl treatment (medium salt stress level for sunflower) was determined. An inhibitory analysis of the NaCl-induced potassium flux showed that it was only 15 % inhibited by specific potassium channel antagonists (Ba2+ ). This indicates a low proportion of potassium channels and the dominant role of non-selective cation channels in providing NaCl-induced potassium loss in Helianthus annuus L. plants.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124549415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Darya Y. Straltsova, M. Charnysh, P. Hryvusevich, V. Demidchik
{"title":"Non-genomic effects of steroid hormones: role of ion channels","authors":"Darya Y. Straltsova, M. Charnysh, P. Hryvusevich, V. Demidchik","doi":"10.33581/2521-1722-2019-3-3-12","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-3-12","url":null,"abstract":"In animals, steroid hormones can act using genomic and non-genomic mechanisms. Plant steroid hormones, brassinosteroids, are capable of inducing the expression of some gene ensembles, however their non-genomic pathways for triggering the physiological effects are still unclear. In this paper, we propose the hypothesis on existence of brassinosteroid non-genomic effects in plant cells. This non-genomic pathway could due to modulation of ion channel activities and modification of membrane receptors.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126849966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The antifungal activity of ray fungum against the fusarium wilt causal agent of tomato","authors":"S. G. Sidorova","doi":"10.33581/2521-1722-2019-3-21-32","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-21-32","url":null,"abstract":"The antifungal activity of genus Streptomyces strains (from collection of department of microbiology, Belarusian State University) against the fusarium wilt causal agent of tomato micromycetes Fusarium oxysporum f. lycopersici (Sacc.) Snyder and Hansen were studied in laboratory model experiments. Dynamic of micromycete colonies growth, morphology, color and fruiting intensity were analyzed. The screening of tested strains of genus Streptomyces for their anti-wilt activity has showed that the strain 10 influences the inhibiting (more than 60 %) effect on all fusaruim isolates: high-pathogenic (Fol 1), middle-pathogenic (Т 2) and low-pathogenic (Т 11). Their colonies growth were blocked from the 4th day of joint cultivation. The strain 11 has been an antagonist for the isolates Fol 1 and T 2, and the strain 20 – for the isolate T 11. Strains 18 and 35 demonstrate no antifungal activity to all F.oxysporum f. lycopercisi isolates under study.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128725348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intra- and interpopulation variability of Solidago canadensis L. s. l. in Belarus","authors":"V. Tikhomirov, Iryna A. Ravenskaya","doi":"10.33581/2521-1722-2019-3-67-78","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-67-78","url":null,"abstract":"The study of the variability of 22 morphological characters in 5 populations of Solidago canadensis L. s. l., growing in the city of Minsk and its environs, was carried out. Comparison of the obtained data with previously published information on the nature and range of variability of the North American taxa Solidago subsect. Triplinerviae showed that plants growing on the territory of the Republic of Belarus cannot be unambiguously assigned to any of the North American species by the combination of morphological characters. Compared to North American taxa, the plants of the studied populations have a significantly more powerful habitus (a large average plant height, longer and in many populations broader mid stem leaves, most often a larger inflorescence), intermediate in comparison with S. canadensis s. str. and S. altissima s. str. and significantly different from both North American species, the length of involucres and the length of tubular flowers, significantly longer outer phyllaries, shorter straps of ray florets, larger ovaries of ray florets, but their shorter pappus, a significantly smaller number of disc florets. The disc florets have significantly larger teeth compared to S. canadensis s. str. and S. altissima s. str. The data obtained confirm the point of view of Hildemar Scholz, according to which the invasive goldenrod, which is currently widely distributed throughout Europe, are of European origin. Most likely, they are either stabilized hybrids that arose during breeding due to the crossing of several North American species, or arose as a result of spontaneous mutation and further selection under natural conditions.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122105458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological changes in the rat’s liver of different age after administration of magnesium chloride","authors":"R. Yanko, E. Chaka, I. G. Litovka, M. Levashov","doi":"10.33581/2521-1722-2019-3-40-48","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-40-48","url":null,"abstract":"The aim of the work was to study the morphological changes in the rat’s liver of different ages after prolonged administration of magnesium chloride. The experiments were performed on 48 Wistar male rats at 3 and 15 months of age. Experi mental animals, in addition to the standard diet, received magnesium chloride daily for 21 days at a dose of 50 mg / kg of body weight. Histological preparations were made from the liver tissue according to the standard method. The liver morphometry was performed on digital images using the computer program Image J. The content of magnesium cations was determined in the serum and suspension of red blood cells. Based on the results of our studies, it can be assumed that the administration of magnesium chloride activates the processes of physiological regeneration and functional activity of the liver parenchyma in rats of different ages. This is evidenced by an increase in the number of binuclear cells and nucleolus in the nucleus of hepatocytes, an increase in the nuclear-cytoplasmic and nucleolar-nuclear ratio. The relative area of the sinusoid network, the number and density of connective tissue cells were increased in the liver of 3-month-old experimental rats. This may indicate an increase of trophic and protective activity of the stroma in this organ. Experimental rats (15-month-old animals) revealed a moderate increasing in the erythrocyte suspension and blood serum magnesium content. The administration of magnesium chloride has the morphological features indicating an increasing of the physiological regeneration and activity of the liver parenchyma in young (to a greater extent) and adult animals.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131690828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of algoflora of sod-podzolic sand soil in the conditions of its cultivation","authors":"Eugene E. Gaevskii, Ya. K. Kulikov","doi":"10.33581/2521-1722-2019-3-79-91","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-3-79-91","url":null,"abstract":"As a result of studies of the taxonomic composition and quantitative development of soil algae on sod-podzolic sandy soil of varying degrees of optimization 45 species of soil algae were identified that belong mainly to four divisions: Chlorophyta, Cyanophyta, Bacillariophyta, Xanthophyta. Also in the soil were found not numerous representatives of divisions Euglenophyta and Rhodophyta. Different departments are represented unequally. Most of the found representatives we edaphophilic algae, the participation of hydrophilic algae in the formation of algogroups at the studied sites was insignificant (represented by one species of Cosmarium undulatum var. minutum Wittr.). No amphibial algae were found. The species composition and the spectrum of life forms in different versions of the experiment varied. The control variant of the experiment was represented by the smallest number of species; there are also no nitrogen-fixing cyanobacteria in the soil.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127694657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilya M. Ilyushonak, A. Migas, A. Sukhareuski, Aksana Schneider, V. Grinev
{"title":"The contribution of various mechanisms to mRNA diversity of human fusion oncogene RUNX1-RUNX1T1","authors":"Ilya M. Ilyushonak, A. Migas, A. Sukhareuski, Aksana Schneider, V. Grinev","doi":"10.33581/2521-1722-2019-2-45-59","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-2-45-59","url":null,"abstract":"In this work, we used a comprehensive set of the fusion oncogene RUNX1-RUNX1T1 alternative exons to analyze the patterns of its mRNA generation. We found that the waste majority of alternative exons are modified variants of canonical exons, and the transcripts, including such exons, have a very low expression level. The «hot regions», including exons 4a, 6, 8b, 9, 11 and 12, produces about 80 % of such variants. Also we described a new transcription start region of RUNX1-RUNX1T1 and provide the evidences of co-expression of the fusion RNAs with normal and shortened 3′-UTRs in leukemic cells.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116217012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of endogenous elicitor peptides in plant resistance to biotic stress","authors":"Halina G. Filiptsova","doi":"10.33581/2521-1722-2019-2-3-12","DOIUrl":"https://doi.org/10.33581/2521-1722-2019-2-3-12","url":null,"abstract":"Plant elicitor peptides (Peps) are one class of elicitor substances, which are formed in plant cells in response to various biotic stressors and induced of nonspecific plant resistance. They are present and active in angiosperms, including many important agricultural crops, and can be considered as a promising class of compounds for creating environmentally safe drugs that induce phytoimmunity and increase the resistance of plants to stress. In this paper, an analysis of current literature data on the functional activity of endogenous plant elicitor peptides, the mechanisms of Pep-signaling and their role in plant resistance to biotic stresses is carried out.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130503102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}