Pelita Perkebunan (a Coffee and Cocoa Research Journal)最新文献

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Frequency distribution and potence ratio of agronomic traits in F1 generation of intraspecific crosses of Robusta coffee. 罗布斯塔咖啡种内杂交 F1 代农艺性状的频率分布和效力比。
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-08-15 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.495
A. Wibowo, Ucu Sumirat
{"title":"Frequency distribution and potence ratio of agronomic traits in F1 generation of intraspecific crosses of Robusta coffee.","authors":"A. Wibowo, Ucu Sumirat","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i2.495","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.495","url":null,"abstract":"Leaf morphological traits and internode length significantly affect the adapt-ability and production of coffee plants, respectively. Therefore, genetic studies of these two characters can support the effectiveness of Robusta coffee breeding program for obtaining superior planting materials. This study aimed to determine the diversity and frequency distribution of F1 generation on internode length and leaf size of Robusta coffee crosses. The study involved six groups of F1 progeny and their reciprocals (F1R) resulted from a cross of three parents, namely BP 961, Q 121, and BP 409. Each cross combination was planted with 41–49 progenies singly side by side in Kaliwining Experimental Station, Jember, Indonesia. The observed traits were internode length and leaf size. Analysis results of progeny variation distribution showed that most of the F1 and F1R progenies from intraspecific Robusta crosses had shorter branches than both parents in all combinations of crosses, even in BP 961 × BP 409 crosses. In addition, all F1 and F1R progenies had shorter internodes than both parents. More than 50% of the progeny of Q 121 × BP 409 and BP 961 × BP 409 crosses showed leaf length trait between parents. This indicates the absence of dominance of one parent in the progeny. Less than 20.9% of the progeny had a narrower leaf size than Q 121 parent. Most of the F1 and F1R crosses had a leaf width between both parents. The leaf length and leaf width traits were incomplete dominance or recessive, while the internodelength was overdominant. ","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127643589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adaptation of introduced Robusta coffee clones in some agroclimate types in East Java. 引进罗布斯塔咖啡无性系在东爪哇某些农业气候类型中的适应性。
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-08-15 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.323
Pingkan Sugianto, S. Sudarsono, D. Sukma, Ucu Sumirat
{"title":"Adaptation of introduced Robusta coffee clones in some agroclimate types in East Java.","authors":"Pingkan Sugianto, S. Sudarsono, D. Sukma, Ucu Sumirat","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i2.323","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.323","url":null,"abstract":"Indonesia is the fourth largest coffee producer in the world, even though it is not the original plant. Efforts to increase coffee genetic diversity in Indonesia through the results of the introduction are also carried out to improve the quality and quantity of coffee in the international market. The aim is to obtain robusta coffee clones that are able to adapt in several agroclimates in Indonesia and have stable yield potential, so that they can be cultivated extensively. The analytical method is AMMI biplot with six clones are FRT04, FRT06, FRT07, FRT09, FRT23, FRT65, and six locations are Bangelan, Kalibendo, Kaliselogiri, Gumitir, Malangsari, Silosanen.Then the agroclimate is suitable for widespread cultivation. The results of the study based on observations made on the production of coffee plants, which are climate types, which are somewhat wet and are getting good production results in the locations of Bangelan, Kaliselogiri and Silosanen. FRT07and FRT09 clones is the best clone of production and able to adapt, has interaction between locations with clones tested annually.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125591660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica 阿拉比卡咖啡胚性诱导初生愈伤组织的建立
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-08-15 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.510
R. Arimarsetiowati, B. Daryono, Y. Astuti, E. Semiarti
{"title":"Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica","authors":"R. Arimarsetiowati, B. Daryono, Y. Astuti, E. Semiarti","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i2.510","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.510","url":null,"abstract":"Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7˚55'' ' S, 113˚41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal  and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132082610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Flush characteristics of several cocoa genotypes different in resistant to vascular streak dieback 几种不同抗维管条纹枯病基因型的同色特性
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-08-15 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.518
I. A. Sari, B. Setyawan, Agung Wahyu Susilo, Nurhadini Fitri Isnaini, Samsul Paputpungan, Febrilia Nur'aini, Nur Solecha Ruseani
{"title":"Flush characteristics of several cocoa genotypes different in resistant to vascular streak dieback","authors":"I. A. Sari, B. Setyawan, Agung Wahyu Susilo, Nurhadini Fitri Isnaini, Samsul Paputpungan, Febrilia Nur'aini, Nur Solecha Ruseani","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i2.518","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.518","url":null,"abstract":"Vascular streak dieback (VSD) is one of the main diseases on cocoa that can cause a decrease in production and even death on susceptible plants. The use of selection criteria is very important in the selection process at the seedling phase, young plants and even mature plants in order to support the breeding process of resistant varieties. The aim of the study was to determine the characteristics of flush including flush color, stomata characters and duration of flush color change to green or towards mature leaves as one of the selection indicators for VSD resistance in cocoa. The research was conducted at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute, Jember, Indonesia. Study of flush color and stomata characters was conducted using a randomized complete block design consisting of four cocoa genotypes with different levels of resistance, classified as resistant to VSD disease (Sulawesi 1, PNT 16) and as susceptible (BCL and BL 50). Each clone was repeated three times and each replication consisted of three plant samples. Flush color duration study was carried out on six cocoa genotypes as treatments, three genotypes with resistant to VSD (PNT 16, Scavina 12, Sulawesi 1) and three genotypes as susceptible to VSD (BL 50, BCL, Criollo 22). Each treatment was repeated three times and each replication consisted of three plant samples. The results showed that the resistant genotypes showed a tendency to have lower chlorophyll and anthocyanin content than the susceptible ones. The stomata character in the resistant genotypes was not different than the susceptible ones, however, the resistant genotype showed that the stomata density at flush tended to be lower. The duration of flush color change to mature leaves (green) in resistant genotypes was significantly faster than susceptible genotypes.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121324355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Yield Performance Evaluation of Arabica Coffee Progenies Resulted from Three Way Cross Method 三向杂交法对阿拉比卡咖啡后代产量性能的评价
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-20 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.493
M. R. Akbar, A. Wibowo, Ucu Sumirat, Dwi Nugroho, Muhammad Fuad Anshori
{"title":"Yield Performance Evaluation of Arabica Coffee Progenies Resulted from Three Way Cross Method","authors":"M. R. Akbar, A. Wibowo, Ucu Sumirat, Dwi Nugroho, Muhammad Fuad Anshori","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.493","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.493","url":null,"abstract":"Breeding for high yielding, pest and disease resistance, and good quality Arabica coffee is the direction of the coffee breeding program in Indonesia. Threeway cross-breeding method becomes the alternative method to improve plant genetics to achieve those goals. This research was aimed to evaluate the agronomic performance and grouping of Arabica coffee genotypes from three-way cross progeny. This study was conducted in Andungsari Experimental Station, Bondowoso, East Java from July until September 2021. A randomized complete block design with three replications was used as an experimental design. It consisted of eight Arabica genotypes resulting from three-way cross method and one check variety (Andungsari 1) as treatments. The tested genotypes were resulted from crossing parents i.e. S 1934, AB 3, RC, YC, S 795, and Catimor that was planted in 1997. The results of the agronomic performance evaluation showed that TWC (Tree Way Cross) 2 ((S 1934/AB 3 x AB 3) x AB 3) and TWC 3 (RC/S 795 x Catimor) had good agronomic characteristics i.e. weight of 100 cherries and cherry weight per tree. While grouping using heatmap clustering method showed that there were three groups of genotypes. One of the groups which consisted of TWC 1 ((S 1934/AB 3 x AB 3) x S 1934), TWC 2 ((S 1934/AB 3 x AB 3) x AB 3), and TWC 5 (S 1934/YC x S 1934) were categorized as moderate yield group.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127498156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Heritability and Combining Ability of Some Vegetative and Yield Characteristics of Promising Arabica Coffee Varieties in Indonesia 印度尼西亚有前途的阿拉比卡咖啡品种的一些营养和产量特征的遗传力和配合力
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-20 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.484
A. Wibowo, M. R. Akbar, Ucu Sumirat
{"title":"Heritability and Combining Ability of Some Vegetative and Yield Characteristics of Promising Arabica Coffee Varieties in Indonesia","authors":"A. Wibowo, M. R. Akbar, Ucu Sumirat","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.484","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.484","url":null,"abstract":"Breeding for high yielding and good quality arabica coffee is the direction of the coffee breeding program in Indonesia. Hybridization becomes the important step to select promising genotypes from the genetic variability to achieve those goals. This research aimed to study heritability and combining ability of vegetative and yield characteristics of arabica coffee offspring. This research was conducted in randomized complete block design with three replications at Andungsari Experimental Station, East Java. The materials consisted of five parents of arabica coffee namely BP 542A(P1), P88(P2), S795(P3), HDT(P4), GEISHA(P5), and ten F1 hybrids as the offspring. The results showed that the narrow-sense heritability of leaf width was categorized as high. P3 and P5 varieties had the highest general combining ability (GCA) value for internode length, cluster number, and cherries number. These results indicated that both parental lines had potential as parents for the breeding program. The highest value of specific combining ability (SCA) for cherry size and leaf size was shown in crossing combination of P1×P3 and P2×P3. While, the crossing combination of P3×P4 showed the highest SCA value for cluster number, internode length, and cherries number per branch. From this crossing combination, high yielding promising lines can be obtained.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132178474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing the Impact of Illegal Small-Scale Mining (Galamsey) on Cocoa Farming and Farmer Livelihood: A Case Study in the Amansie West District of Ghana 评估非法小规模采矿(Galamsey)对可可种植和农民生计的影响:以加纳Amansie West地区为例
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-20 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.496
Najat Osman, John Tennyson Afele, E. Nimo, David Ofoe Gorleku, Louisa Adomaa Ofori, A. Abunyewa
{"title":"Assessing the Impact of Illegal Small-Scale Mining (Galamsey) on Cocoa Farming and Farmer Livelihood: A Case Study in the Amansie West District of Ghana","authors":"Najat Osman, John Tennyson Afele, E. Nimo, David Ofoe Gorleku, Louisa Adomaa Ofori, A. Abunyewa","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.496","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.496","url":null,"abstract":"Cocoa and gold are significant contributors to the gross dormestic product of Ghana which makes both commodities key parts of the Ghanaian economy. Unfortunately, in Ghana, most cocoa-growing communities are the hub of illegal small-scale mining commonly known as galamsey. It is alleged that cocoa farmerssell their farmlands for galamsey; however, given that cocoa farming is the primary source of income for many households in rural Ghana. This research aimed at investigating the motivations for galamsey and how the practice impacts cocoa farming and the environment as well as farmer livelihood. The study was undertakenin Amansie West District in the Ashanti Region of Ghana where both cocoa farming and illegal mining operations coexist. The data for the study was collected using structured questionnaires. Cocoa farmers were purposively selected as respondents in two cocoa-growing communities. Data were obtained from 90 purposively sampled respondents through snowballing. Data collected were analyzed with Statistical Package for Social Scientist software. The results revealed that cocoa farmers face challenges due to galamsey such as; labour shortages, water pollution, competition for land amongst others. Also, cocoa farms, especially those downslope, get flooded whenever it rains as a result of uncovered galamsey pits near farms and cause black pod disease, early dropping of immature pods and stunted growth which ultimately reduce the output of cocoa farms. Farmers revealed that this affected their source of income adversely since the majority of them mainly depend on cocoa farming for their livelihood. They also reported a loss of green space and land degradation as some environmental hazards posed by galamsey. Information gathered from tenant farmers revealed that it is mostly landowners who give out their lands for galamsey. The major reasons that encourage galamsey were identified as higher short-term income, financial problems (40%) due to unemployment, and poor cocoa yield (26%). ","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"519 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123127901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Temperature and pH Monitoring System Design in the Fermentation of Cocoa Beans Based on Android 基于Android的可可豆发酵过程温度和pH监测系统设计
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-20 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.494
L. H. Pratopo, A. Thoriq, E. Purwanto, Daffa Afian Wiradwinanda
{"title":"Temperature and pH Monitoring System Design in the Fermentation of Cocoa Beans Based on Android","authors":"L. H. Pratopo, A. Thoriq, E. Purwanto, Daffa Afian Wiradwinanda","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.494","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.494","url":null,"abstract":"The fermentation of cocoa beans is a key step in increasing their quality. Temperature and pH have an impact on the fermentation process' performance. Until, temperature and pH measurements in the cocoa bean fermentation process have been done with a thermometer and a pH meter, and have been heavily impacted by experience and knowledge. The goal of this research is to create a temperature and pH monitoring system for the fermentation of cocoa beans using an android platform. The sensor must be able to measure temperature and pH during the fermentation process, and the sensor reading data must be displayed on the android display, according to the system design criteria. A temperature sensor, pH sensor, microcontroller, TP-Link or WiFi modem, ThingSpeak (data storage), and Smartphone make up the system's hardware. Arduino CC software version 1.8.14 was used to create the software. The system hardware was made up of numerous components, including the NodeMCU ESP32 microcontroller, SKU SEN0161 pH sensor, DS18B20 temperature sensor, 4.2k resistor, jumper cable, and 5 volt charger adaptor, according to the results. A plastic container houses the electronic system circuit. The monitoring system can read the temperature and pH during the fermentation process, which lasts 112 hours or 6 days, according to the performance test findings. During fermentation, the temperature of cocoa beans ranged from 27.19 °C to 41.75 °C, with the pH fluctuating between 2.50 and 7.10.","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130353095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physical, Chemical, and Sensory Properties of Robusta Coffee Effervescent Tablets Formulated in Various Organic Acids 罗布斯塔咖啡泡腾片在各种有机酸中配制的物理、化学和感官特性
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-20 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.489
U. H. Hasbullah, Miftahul Wahidatun Ni’mah, E. Retnowati, R. Umiyati
{"title":"Physical, Chemical, and Sensory Properties of Robusta Coffee Effervescent Tablets Formulated in Various Organic Acids","authors":"U. H. Hasbullah, Miftahul Wahidatun Ni’mah, E. Retnowati, R. Umiyati","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.489","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.489","url":null,"abstract":"Coffee effervescent products are an innovation in coffee formulation. The compounds that play a role in effervescent are acids and bases. Type of organic acid give an impact on the effervescent characteristics. This study aimed to examine the effect of type of organic acid on physical, chemical, and sensory properties ofRobusta coffee effervescent tablets. This study used a completely randomized design with three acids in the formulation, namely citric acid, tartaric acid, and malic acid. Samples were analyzed in three replications. Making effervescent tablets was done by compression technique in a mixture of all ingredients according tothe formula. The results showed that different acid had a significant effect on physical and chemical parameters. Malic acid caused a faster effervescent time than citric acid and tartaric acid. Malic acid and tartaric acid tended to lower the pH slightly than citric acid. Malic acid and citric acid tended to produce hardertablets than tartaric acid. However, tartaric acid slightly increased tablets’ brightness (L*) compared to malic acid and citric acid. Tartaric acid and malic acid tended to reduce moisture compared to citric acid. The IC50 value of effervescent with malic acid and tartaric acid was lower than that of citric acid. However, therewas a slight decrease in total phenol in both. Meanwhile, the sensory profiles of tablets and effervescent drinks did not differ due to different acids. The recommended formula was that the effervescent using malic acid had an effervescent time of 166 seconds, hardness 321 N, moisture 8%, IC50 5.5 mg mL-1, total phenol4.2 mg gallic acid equivalent (GAE) g-1, and a drink profile that has the best color, aroma, taste, and runs time","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127493925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Cocoa Clonal Seedlings Purity Through Deoxyribonucleic Acid (DNA) Barcoding and Random Amplification of Polymorphic DNA (RAPD) Fingerprinting 利用脱氧核糖核酸(DNA)条形码和随机扩增多态性DNA (RAPD)指纹图谱分析可可克隆苗纯度
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Pub Date : 2022-04-12 DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i1.490
Sulistyani Pancaningtyas, Agung Wahyu Susilo
{"title":"Analysis of Cocoa Clonal Seedlings Purity Through Deoxyribonucleic Acid (DNA) Barcoding and Random Amplification of Polymorphic DNA (RAPD) Fingerprinting","authors":"Sulistyani Pancaningtyas, Agung Wahyu Susilo","doi":"10.22302/iccri.jur.pelitaperkebunan.v38i1.490","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i1.490","url":null,"abstract":"The genetic purity of a plant indicates the similarity properties between seedlings in the field and the description of the plant in the database. Identifying plant purity through a morphological approach has several drawbacks, including time efficiency and environmental factors, and the diversity is limited and inconsistent. This condition encourages the development of detection methods using DNA molecular markers. Plant identification through fingerprinting or the use of molecular markers has not been widely carried out on a commercial scale, considering the investment costs for this analysis are still quite expensive. Another method used for plant identification through DNA Barcoding by comparing variations between DNA sequences. The primers used to identify barcodes on cocoa were derived from the chloroplast genome, including rbcL and matK. This research aims to see the consistency of the rbcL primer when applied to other cocoa clones, and the analysis of the polymorphic diversity of each cocoa clone using DNA fingerprinting RAPD. This method will be tested on clones of Sulawesi 1, Sulawesi 2, ICCRI 03, and ICCRI 09, which were propagated by SE and mother plants in the field using cocoa leaf samples. The stages include DNA extraction, sequencing, and analysis of the sequencing results. The results of seedlings uniformity analysis using DNA barcoding on cocoa plants produced from in vitro propagation showed that the multiplied seeds did not show any difference in sequence with the parent plant (DR2, Sulawesi 1, Sulawesi 2, and ICCRI 09). The analysis of the diversity of cocoa clones DR 2, MCC 2, Sulawesi 1, Sulawesi 2, and ICCRI 09 through DNA Fingerprinting RAPD showed that the OPA 15 primer produced a more apparent polymorphic band than the other three primers (OPP 08, OPW 11, and M 29).","PeriodicalId":428979,"journal":{"name":"Pelita Perkebunan (a Coffee and Cocoa Research Journal)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124095680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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