Universa Medicina最新文献_第4页

Influence of interspecies interactions on biomass and extracellular polymeric substances of bacterial biofilms 种间相互作用对细菌生物膜生物量和细胞外聚合物的影响

IF 0.3

Universa Medicina Pub Date : 2023-04-10 DOI: 10.18051/univmed.2023.v42.84-93

Mercedes Baltazara Schettini Alava, N. R. Maddela

{"title":"Influence of interspecies interactions on biomass and extracellular polymeric substances of bacterial biofilms","authors":"Mercedes Baltazara Schettini Alava, N. R. Maddela","doi":"10.18051/univmed.2023.v42.84-93","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.84-93","url":null,"abstract":"BackgroundStudies with emphasis on pure and mixed-species biofilms are of significant importance in understanding biofilm formation mechanisms during microbial infections. This research aims to evaluate pure- and dual-species biofilms of Escherichia coli (code A), Staphylococcus aureus (code B), Klebsiella pneumoniae (code C) and Pseudomonas aeruginosa (code D) pathogenic bacterial species and their production of biofilm exopolysaccharides at laboratory scale.\u0000MethodsBiofilm biomass (A595) of pure- and dual-species cultures was determined by means of a microtiter plate assay in triplicate using a microplate photometer (Fisher Scientific, type-357). Extracellular polymeric substances (EPS) and soluble microbial products (SMP) were extracted from the biofilm cells (pure- and dual-species cultures) using the alkaline-heat extraction method. Dry weights (g/L) of EPS and SMP were determined by drying the samples at 105 °C for 8 hours.\u0000ResultsKlebsiella pneumoniae biofilm biomass accounted for a 28-72% greater biofilm biomass than the other bacteria. Experimental values of dual-species biofilm biomasses were in the range of 6% to 30% over theoretical values. The experimental value of one dual-species (bacteria B + D) biofilm biomass was 30% higher than its expected value. Decrease or increase in the dual-species biofilm biomass of either bacteria A+C or bacteria B+C combinations was totally dependent on the cell density of bacteria C.\u0000Conclusions Biofilm biomass of pure-species cultures was totally species-dependent, and the biofilm biomass of four species was in the following order: bacteria C > D > A > B. Relation between biofilm biomasses and SMP or EPS was inconsistent.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43195928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Headache in healthcare workers related to personal protective equipment use in COVID-19 referral hospital 与COVID-19转诊医院使用个人防护装备相关的卫生保健工作者头痛

IF 0.3

Universa Medicina Pub Date : 2023-04-06 DOI: 10.18051/univmed.2023.v42.52-60

Ida Ayu Sri Wijayanti, Ni Putu Ayu Putri Mahadewi, P. Sudira, K. Tini, N. Susilawathi, I. Adnyana

{"title":"Headache in healthcare workers related to personal protective equipment use in COVID-19 referral hospital","authors":"Ida Ayu Sri Wijayanti, Ni Putu Ayu Putri Mahadewi, P. Sudira, K. Tini, N. Susilawathi, I. Adnyana","doi":"10.18051/univmed.2023.v42.52-60","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.52-60","url":null,"abstract":"BackgroundStudies show that wearing personal protective equipment (PPE) for long periods of time can lead to discomfort such as headaches, which could affect the performance of healthcare workers. The aim of this study was to determine the prevalence and risk factors of headaches related to PPE in healthcare workers at a COVID-19 referral hospital.\u0000MethodsA cross-sectional study was conducted involving 174 healthcare workers in a COVID-19 referral hospital in Bali. We conducted interviews using a questionnaire that consisted of three main parts: characteristics of the subjects, PPE usage, and PPE-associated headaches. A multiple logistic regression was used to analyze the data.\u0000ResultsThe analysis results showed that the PPE-associated headaches had a prevalence of 63.8% and were gradual in onset, pressure-like in quality (46%), and mild in intensity (80.1%). PPE level III-associated headache was the most common type. The majority of the participants had headaches up to 6 hours after using the protective gear, but improving within 15-30 minutes of removal and/or after pharmacotherapy. A Chi-squared analysis showed a statistically significant association between duration of PPE use, working units, and PPE levels (p<0.05). A logistic regression analysis found a significant relationship between PPE level and headache occurrence (OR=4.826;95%CI: 2.433-9.572; p<0.001).\u0000ConclusionThe frequency of PPE-associated headache was high and the PPE level was a risk factor of headache among healthcare workers. Better strategies are needed to reduce the duration of PPE exposure so that the work performance and quality of life of healthcare workers are not significantly affected.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48016395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental growth factor as a screening tool of preeclampsia 胎盘生长因子作为子痫前期筛查工具的研究

IF 0.3

Universa Medicina Pub Date : 2023-03-30 DOI: 10.18051/univmed.2023.v42.4-5

Raditya Wratsangka

引用次数: 0

Determinants of coronary heart disease among adults: a case-control study 成人冠心病的决定因素:一项病例对照研究

IF 0.3

Universa Medicina Pub Date : 2023-03-16 DOI: 10.18051/univmed.2023.v42.41-51

Sudikno Sudikno, Srilaning Driyah, Julianty Pradono

{"title":"Determinants of coronary heart disease among adults: a case-control study","authors":"Sudikno Sudikno, Srilaning Driyah, Julianty Pradono","doi":"10.18051/univmed.2023.v42.41-51","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.41-51","url":null,"abstract":"BackgroundAccording to recent World Health Organization data, coronary heart disease (CHD) remains the leading cause of death worldwide. Although the risk factors of this disease are well known, the strength of these factors varies in different populations. The aim of the study was to assess the determinants of CHD in Indonesian adults aged 25 years and over.\u0000MethodsA case–control study was carried out involving 592 subjects aged 25 years and over (444 controls and 148 cases). Participants were interviewed using validated questionnaires. Physical examinations and supporting examinations were conducted. The Chi square test, Fisher’s exact test, and independent t test were used to analyze the data. To determine independent predictors of CHD, the odds ratio (OR) was determined via the multiple logistic regressions test.\u0000ResultsThe CHD subjects (case group) showed higher blood sugar and systolic pressure than non-CHD subjects (controls), with mean fasting blood sugar of 92.53±27.05 mg/dL vs 88.29 ±23.43 mg/dL (p=0.038), 2-hour postprandial blood sugar of 133.15±65.09 mg/dL vs 120.87±44.60 mg/dL (p=0.000), and systolic blood pressure of 89±27.62 mmHg vs 129.98±10.58 mmHg (p=0.002). The logistic regression analysis in the case group showed that higher education (college) had a 2.32-fold greater effect (95% CI. 1.01-5.35) on CHD incidence compared to the control group.\u0000ConclusionsThis study has demonstrated that the most frequent risk factor for CVD in adults aged 25 years and over is higher education. Control and prevention of CHD need to be done with regular control of blood sugar levels and blood pressure to stabilize them within normal limits.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47474642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol protects against copper and iron toxicity in Drosophila melanogaster 白藜芦醇对黑腹果蝇铜和铁毒性的保护作用

IF 0.3

Universa Medicina Pub Date : 2023-03-08 DOI: 10.18051/univmed.2023.v42.29-40

O. G. Igharo, Lucky Osemu Ebaluegbeifoh, Godwin Aigbedo AIKPITANYI-IDUITUA, Henry Uzor Oshilonyah, Idris Babatunde Momodu

{"title":"Resveratrol protects against copper and iron toxicity in Drosophila melanogaster","authors":"O. G. Igharo, Lucky Osemu Ebaluegbeifoh, Godwin Aigbedo AIKPITANYI-IDUITUA, Henry Uzor Oshilonyah, Idris Babatunde Momodu","doi":"10.18051/univmed.2023.v42.29-40","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.29-40","url":null,"abstract":"BackgroundCopper (Cu) and iron (Fe) are essential trace elements that when in excess are capable of causing cytotoxic effects leading to lipid peroxidation and promoting oxidative stress. Resveratrol (RES) is a natural polyphenol with antioxidant and anti-inflammatory properties. This study was carried out to evaluate the protective role of RES in Fe and Cu sulphate-induced oxidative stress in Drosophila melanogaster. \u0000MethodsAdult wild type flies were fed Cu2+ and Fe2+ (1 mM each) and/or RES (30 and 60 mg/kg diet) for 7 days. Survival, negative geotaxis and emergence rate were evaluated by daily recording of fruit fly mortality and final analysis. Fruit flies were anaesthetized using CO2 gas, homogenized and centrifuged at 4,000 rpm for 10 minutes at 4 °C. Aliquots of the supernatants were used for the estimation of biochemical markers using spectrophotometry.\u0000ResultsFruit flies co-treated with FeSO4 + CuSO4 (1 mM each) + RES (30 and 60 mg/Kg) significantly elevated H2O2, NO, lipid peroxidation, acetylcholinesterase as well as GSH, GST, catalase and total thiols (p<0.05) compared with the Cu2+ + Fe2+ (1mM each) treated group. Flies co-treated with FeSO4 + CuSO4 (1mM each) + RES (30 and 60mg/Kg) also had significantly improved (p< 0.05) eclosion and climbing rates compared with the Cu2+ + Fe2+ (1mM each) treated group.\u0000ConclusionThis study demonstrated that RES reduced Cu2+ and Fe2+-induced radical generation in D. melanogaster and improved the antioxidant buffering capability of the flies. Therefore, RES could be used in management of disorders involving oxidative stress.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44999863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emotional eating in relation to gastrointestinal symptoms and burnout among young women during the pandemic 大流行期间年轻女性肠胃症状和倦怠与情绪化进食的关系

IF 0.3

Universa Medicina Pub Date : 2023-03-02 DOI: 10.18051/univmed.2023.v42.21-28

G. Yılmazel, Emre Keles, Nurettin Ayaz

{"title":"Emotional eating in relation to gastrointestinal symptoms and burnout among young women during the pandemic","authors":"G. Yılmazel, Emre Keles, Nurettin Ayaz","doi":"10.18051/univmed.2023.v42.21-28","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.21-28","url":null,"abstract":"BackgroundThe emergence of the coronavirus disease (COVID-19), created unique constraints in everyday life. Emotional eating is a known phenomenon in disasters and is markedly associated with gastrointestinal symptoms. In this study, the aim was to assess the relationship of gastrointestinal symptom severity and COVID-19 burnout with emotional eating among young women during the pandemic disaster.\u0000MethodsA cross-sectional study approach was used to allow 462 young women participants in this study. The design of the questionnaires was based on demographics, health behaviors, Gastrointestinal Symptom Severity Scale, Emotional Eating Scale and COVID-19 Burnout Scale. Data were analyzed using percentages, mean values, independent t-test, chi-squared test. The hierarchical multiple regression analysis was performed for predicting risk factors of emotional eating. Significance levels were set at the 5% level.\u0000ResultsOf the women, 73.8% were emotional eaters. The level of COVID-19 burnout was moderate with mean score of 29.4±11.1 and emotional eating total score was 21.0±8.1. Increased number of meals, increased weight gain and shorter sleep time were significantly associated with emotional eating (p<0.05). Participants with more than three meals per day were more likely to be emotional eaters (Beta=4.26). The regression model showed that indigestion and COVID-19 burnout were strong risk factors of emotinal eating (p<0.05).\u0000ConclusionsThis study demonstrated that indigestion and COVID-19 burnout were strong risk factors of emotinal eating. Emotional eating could pose an additional health burden to young women in the form of poor food choices.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41443458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance genes of Neisseria gonorrhoeae to cefixime and azithromycin 淋球菌对头孢克肟和阿奇霉素的耐药性基因

IF 0.3

Universa Medicina Pub Date : 2023-02-22 DOI: 10.18051/univmed.2023.v42.108-118

Louisa Ivana Utami, Yeva Rosana

{"title":"Resistance genes of Neisseria gonorrhoeae to cefixime and azithromycin","authors":"Louisa Ivana Utami, Yeva Rosana","doi":"10.18051/univmed.2023.v42.108-118","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.108-118","url":null,"abstract":"Gonorrhea is the second most common sexually transmitted bacterial infection (STI), following Chlamydia. Neisseria gonorrhoeae resistant to antibiotics are increasing globally in the world. In recent years, many studies have reported reduced susceptibility of N.gonorrhoeae to almost all clinically useful antibiotics and also reported cases of multi-resistance. Resistance mechanisms for N. gonorrhoeae can occur through genetic and non-genetic changes. Resistance to cefixime and azithromycin as first-line antibiotics for monotherapy recommended by the World Health Organization (WHO) has been reported from several countries. Genetic changes were reported as the main cause of N.gonorrhoeae resistance to cefixime and azithromycin. Based on the WHO and the United States Centers for Disease Control and Prevention recommendations, countries are increasingly using a combination of cephalosporin and azithromycin for the treatment of gonorrhea. The aim of this review is to analyze genetic variation of N.gonorrhoeae resistance to cefixime and azithromycin. Articles published in English in the last 12 years (from 2010 to 2021) were retrieved from Science Direct, PubMed, Springerlink, Oxford and Nature using relevant searching terms. Mutants of cefixime-resistant N.gonorrhoeae are mediated by mosaic and non-mosaic penA genes encoding penicillin binding protein 2. In addition, mutations in the repressor and promoter genes of mtrR were also found that caused overexpression of the microbial efflux pump. Meanwhile, N. gonorrhoeae resistance to azithromycin reportedly occurs through two strategies, namely overexpression of the efflux pump (mutation of the mtrR codon region) and decreased affinity for antibiotics (single base mutation in the 23S rRNA gene). With the limited choice of antibiotics for the management of N.gonorrhoeae, it is necessary to do regular surveillance for monitoring drug resistance. By understanding the mechanism of resistance, the use of these antibiotics can be rationally optimized.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46235073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of probiotic supplementation in cognitive improvement and other metabolic biomarkers 益生菌补充在认知改善和其他代谢生物标志物中的潜在作用

IF 0.3

Universa Medicina Pub Date : 2023-02-17 DOI: 10.18051/univmed.2023.v42.1-3

Phakkharawat Sittiprapaporn

引用次数: 0

Nurses' knowledge, attitudes and practices related to physical restraint: the case from Turkey 护士对身体约束的知识、态度和做法:来自土耳其的案例

IF 0.3

Universa Medicina Pub Date : 2023-01-31 DOI: 10.18051/univmed.2023.v42.12-20

Selen Ozakar Akca, A. P. Turan

{"title":"Nurses' knowledge, attitudes and practices related to physical restraint: the case from Turkey","authors":"Selen Ozakar Akca, A. P. Turan","doi":"10.18051/univmed.2023.v42.12-20","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.12-20","url":null,"abstract":"BackgroundNurses are primarily for the care of patients and should ensure that physical restraint, which has many negative consequences, be applied according to standard practice. Therefore, they should have knowledge about physical restraint and apply physical restraint by observing existing laws and ethical principles. This study aimed to determine the knowledge level, attitude and practices of pediatric nurses on physical restraint and identify the factors influencing physical restraint use.\u0000MethodsA cross-sectional study was conducted involving 150 pediatric nurses working in the pediatric clinic of a training and research hospital. The data were collected with an information form containing the introductory characteristics of the nurses and the Level of Knowledge, Attitudes and Practices of Staff Regarding Physical Restraints Questionnaire. Data were analyzed using the Mann-Whitney U test and the Kruskal-Wallis test.\u0000ResultsOverall, nurses had good restraint-related knowledge with positive attitudes, although the physical restraint practices of nurses were not the best and there were some deficiencies. A statistically non-significant difference was found between age, gender, education status, nurses' work unit, and training status regarding physical restraint on the one hand and knowledge, attitude and practice scores relating to the use of physical restraints on the other (p>0.05).\u0000ConclusionsPediatric nurses' level of knowledge about physical restraint was quite good and their attitudes were positive. However, there were some deficiencies in their practices. Future training should therefore be emphasized to enhance nurses' ethical and sensitive thinking, increase their competence in applying physical restraint and working with patients.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46030510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19 and the ageing immune system in an elderly patient : a case report 新冠肺炎与老年患者衰老免疫系统的病例报告

IF 0.3

Universa Medicina Pub Date : 2023-01-19 DOI: 10.18051/univmed.2023.v42.101-107

I. Aryana, Ida Ayu Pradnya Paramita

{"title":"COVID-19 and the ageing immune system in an elderly patient : a case report","authors":"I. Aryana, Ida Ayu Pradnya Paramita","doi":"10.18051/univmed.2023.v42.101-107","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.101-107","url":null,"abstract":"BackgroundThe severity of COVID-19 infection has an increasing trend in the elderly, which contributes to the high morbidity and mortality rates in this population. Aging itself is a prominent risk factor for severe disease and death from COVID-19.\u0000Case DescriptionThis case report a 71-year-old woman who complained of shortness of breath for 3 days before being admitted to the hospital. Bilateral consolidation and increased bronchovascular pattern were found on chest radiograph, and a positive SARS-COV2 nasopharyngeal swab PCR test result was noted. This patient was diagnosed with confirmed severe manifestation of COVID-19, community-acquired pneumonia and type 1 respiratory failure, as well as type II diabetes mellitus and suspicion of acute gastritis. The results of the geriatric status assessment were moderate functional status, risk of malnutrition, and moderate risk of deep vein thrombosis (DVT). This patient underwent treatment in accordance with the COVID-19 protocol along with management for geriatric status improvement. The patient was given permission to return home after 14 days of treatment, during which time her health had improved and her functional status had changed to moderate dependency. During follow-up, the patient continued to receive therapy. She is still being observed and future evaluations will be conducted.\u0000ConclusionThe increased susceptibility of the elderly to COVID-19 infection is caused by various factors. A burden of death and long-term disability brought on by this pandemic may be lessened by new or modified therapies that target aging-associated mechanisms. Therefore, COVID-19 case management in this population should be done with a comprehensive approach.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45271136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1