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Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold 非表面功能化三维经济生物材料支架的研究
The Applied Biology & Chemistry Journal Pub Date : 2021-09-30 DOI: 10.52679/tabcj-2021-0013
D. Narayanan, S. Bhat, G. Baranwal
{"title":"Characterization of innately decellularised micropattern pseudostem of Musa balbisiana - A non-surface functionalized 3D economic biomaterial scaffold","authors":"D. Narayanan, S. Bhat, G. Baranwal","doi":"10.52679/tabcj-2021-0013","DOIUrl":"https://doi.org/10.52679/tabcj-2021-0013","url":null,"abstract":"Banana (Musa balbisiana) pseudostem 3D scaffolds have been developed here for primary eukaryotic cell and cell line culture as an economical, sustainable, eco-friendly alternative for surface-functionalized polymeric and plant tissue-based structures. Musa pseudostem 3D micro pattern scaffold (MPM-3Ds) developed by freeze-drying followed by ethylene oxide sterilization yielded 5.6ng of DNA per mg of tissue, confirming its extended decellularised state. Thermogravimetric analysis, contact angle measurement, uniaxial testing, and FTIR determined thermal stability, wettability, tensile strength, and surface functional groups respectively. Micro and macronutrients, sugars, and amino acids that naturally enrich MPM-3Ds were estimated using EDAX, HPLC, and biochemical analysis. The most important finding was, non-surface functionalized MPM-3Ds supported attachment, growth, and differentiation of human mesenchyme stem cells, human primary hepatocytes like cells, primary mouse brain cortical neurons, mouse fibroblast cells, and human pancreatic cancer cells. MPM-3Ds showed in vivo biodegradation and biocompatibility in a preliminary analysis in Sprague Dawley rats. These findings illuminate nature's power to nurture cells in the micropattern cradles of MPM- 3Ds that can support innovative research in stem cell differentiation, drug and cosmetic testing, and biosensor development leading to advanced biomedical research.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131565733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Lacunae in the natural origin of severe acute respiratory syndrome coronavirus 2 严重急性呼吸综合征冠状病毒自然起源中的腔隙2
The Applied Biology & Chemistry Journal Pub Date : 2021-09-15 DOI: 10.52679/tabcj-2021-0012
D. Revi
{"title":"Lacunae in the natural origin of severe acute respiratory syndrome coronavirus 2","authors":"D. Revi","doi":"10.52679/tabcj-2021-0012","DOIUrl":"https://doi.org/10.52679/tabcj-2021-0012","url":null,"abstract":"The exact origin of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) responsible for unleashing the pandemic Corona Virus Disease (Covid-19) is still not established unambiguously. The intermediate and reservoir hosts of SARS-CoV-2 need to be identified with clarity and how the disease exploded into a pandemic, inevitability needs urgent scientific answers to contain and prevent future pandemics and crises. This perspective provides awareness of the peculiar features of SARS-CoV-2 and inspects the gaps in the natural zoonotic origin of the pandemic.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132661326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production of bioethanol from amla (Emblica officinalis Gaertn.) 用甘露(Emblica officinalis Gaertn)生产生物乙醇
The Applied Biology & Chemistry Journal Pub Date : 2021-09-15 DOI: 10.52679/tabcj-2021-0011
Minal A. Deolekar, T. Shende
{"title":"Production of bioethanol from amla (Emblica officinalis Gaertn.)","authors":"Minal A. Deolekar, T. Shende","doi":"10.52679/tabcj-2021-0011","DOIUrl":"https://doi.org/10.52679/tabcj-2021-0011","url":null,"abstract":"On account of the increasing demand for valuable herbal products, an attempt was made to produce a functional fermented Ethanol from Amla. This study investigates the potential of ethanol production from Amla (Emblica officinalis Gaertn). In the present study, Amla juice was extracted, filtered, fermented and it shows a suitable medium for the growth of Saccharomyces cerevisiae on yeast peptone dextrose medium for the production of ethanol. Ethanol was separated by fractional distillation and then estimated at 4, 6, 8 and 10 days of the fermentation process by iodometric method for 30oC. The ethanol percentage estimated by the iodometric titration method was high on the 10th day, and it was found to be 1.63 gm% compared to all days. So, the outcome of this study reveals that amla fruit can be used as a crucial constituent for the yield of ethanol with a higher commercial value.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133955870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Doxorubicin hydrochloride liposome and albumin-bound paclitaxel in cancer: a nanotechnology perspective 盐酸阿霉素脂质体和白蛋白结合紫杉醇在癌症中的应用:纳米技术的观点
The Applied Biology & Chemistry Journal Pub Date : 2021-06-26 DOI: 10.52679/tabcj.2021.0010
Rajib Hossain, R. Khan, M. T. Islam, D. Jain, Pracheta Janmeda, Obinna C. Godfrey, Shiwali Bisht, Aakanksha Bharati
{"title":"Doxorubicin hydrochloride liposome and albumin-bound paclitaxel in cancer: a nanotechnology perspective","authors":"Rajib Hossain, R. Khan, M. T. Islam, D. Jain, Pracheta Janmeda, Obinna C. Godfrey, Shiwali Bisht, Aakanksha Bharati","doi":"10.52679/tabcj.2021.0010","DOIUrl":"https://doi.org/10.52679/tabcj.2021.0010","url":null,"abstract":"Nanoparticles (1-100 nanometres in size), products of nanotechnology, offer a modern way to transport anti-cancer drugs by acting as transporters of drugs into tumor cells, hence quenching tumor cell proliferation. Such nanoparticles may be formulated to bind to the tumor cell membrane or inhibit specific reactions of tumor biosynthetic pathway by gene repression, or directly bind to the active sites of essential enzymes in the biosynthetic pathway. Consequently, drugs are completely delivered to the desired cancerous cells without system interference. Liposomal doxorubicin and albumin-bound paclitaxel are two examples of nanotechnologically developed drugs for treating cancer. Modern knowledge of nanotechnology opens up new opportunities for innovative research on cancer therapies and administration and helps minimize harm to healthy cells. This review focuses on the doses and routes of administration of these chemotherapeutic agents used in treating cancers.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121968009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Impact of environmental conditions on the levels of stress and breeding performance in Wistar rats: conventional environment versus environmentally controlled housing. 环境条件对Wistar大鼠应激水平和繁殖性能的影响:传统环境与环境控制的住房。
The Applied Biology & Chemistry Journal Pub Date : 2021-06-19 DOI: 10.52679/tabcj.2021.0009
H. Vs
{"title":"Impact of environmental conditions on the levels of stress and breeding performance in Wistar rats: conventional environment versus environmentally controlled housing.","authors":"H. Vs","doi":"10.52679/tabcj.2021.0009","DOIUrl":"https://doi.org/10.52679/tabcj.2021.0009","url":null,"abstract":"It has been established that providing comparable and standard environmental conditions to house experimental rats is of high importance. However, there is growing evidence that rats prefer higher temperatures owing to their thermoneutral zone that lies between 28-34°C. This experiment validates the stress levels of Wistar rats of both sexes housed in conventional conditions about 24-31°C and a relative humidity of 70-92% in a tropical animal facility when compared to rats housed in controlled temperatures of 20-24°C with humidity maintained between 30-70%. Adequate air-changes to maintain ammonia-free environment was provided with a power-exhaust system in a conventional setup and with the individually ventilated caging system in the environmentally controlled group. To assess stress, faecal corticosteroid metabolite assay was done in naïve animals and in a set of animals that underwent retro-orbital bleeding under general anaesthesia. Mothering ability, fecundity and preweaning mortality were also compared between animals housed in two different conditions. Results showed no differences in stress hormone levels between the groups. However, faecal weights differed between males and females in both naïve and orbital bled groups. Animals housed in controlled environment clearly had better breeding data with lesser preweaning mortality in comparison to the conventionally housed rats. Further studies are required to elucidate whether the results are comparable when conducted during all seasons of the year at different locations across the globe.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124254318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyanobacterial biofertilizers as an alternative to chemical fertilizers in paddy fields: a review. 蓝藻生物肥料在稻田中替代化学肥料的研究进展。
The Applied Biology & Chemistry Journal Pub Date : 2021-06-18 DOI: 10.52679/tabcj.2021.0008
A. A. Atnoorkar
{"title":"Cyanobacterial biofertilizers as an alternative to chemical fertilizers in paddy fields: a review.","authors":"A. A. Atnoorkar","doi":"10.52679/tabcj.2021.0008","DOIUrl":"https://doi.org/10.52679/tabcj.2021.0008","url":null,"abstract":"In India, rice is the most significant crop in terms of the total area of cultivation and fertilizers. Distributed in 44.7 million hectares, it accounts for 31.8% (5.34 million tons) of the net use of the chemical fertilizer. Continuously using chemical fertilizers in agricultural production poses a severe environmental risk. The cost of chemical fertilizers is expensive and contributing to less crop yield. There is a need to adopt alternatives for chemical fertilizers in paddy fields. Cyanobacteria are abundant in paddy fields, and it presents remarkable contribution in producing the rice crop. It fixes atmospheric nitrogen, increases the accessibility of available phosphorus, and produces innumerable plant growth-promoting factors. It requires sunlight as the source of energy for carbon and nitrogen fixation in the soil. It represents remarkable potential as a biofertilizer and thereby decreases fuel demand for chemical fertilizer production. Cyanobacterial biofertilizers are inexpensive, simple to use, and do not harm the environment. This review focuses on the potential application of cyanobacterial biofertilizers in paddy fields.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114712829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Bio-genesis and deregulation of circular ribonucleic acid and their role in human cancer 环状核糖核酸的生物发生和解除管制及其在人类癌症中的作用
The Applied Biology & Chemistry Journal Pub Date : 2020-12-27 DOI: 10.52679/TABCJ.2020.0010
Rajakishore Mishra
{"title":"Bio-genesis and deregulation of circular ribonucleic acid and their role in human cancer","authors":"Rajakishore Mishra","doi":"10.52679/TABCJ.2020.0010","DOIUrl":"https://doi.org/10.52679/TABCJ.2020.0010","url":null,"abstract":"RiboNucleic Acid (RNA) occupies the center position in the central dogma of molecular biology. These are the nucleotide with a ribose sugar and are found either in linear or circular form. The linear RNAs are of different types and include ribosomal RNA (rRNA), messenger RNA (mRNA), transfer RNA (t-RNA), small nuclear (snRNA) RNA, and very small/micro RNA (microRNAs). The circular (circRNA) RNA is a group of noncoding RNA, stable molecules, established recently and linked with the regulation of different genes, RNAs including microRNAs. The current understanding of these molecules suggests that these circRNAs are fairly conserved and show tissue-specific expression patterns. These molecules are connected with different pathogenic conditions and associated with verities of diseases, including cancer. CircRNAs are thus contributing to tumorigenesis, and these molecules show the potential to become future predictive biomarkers for diagnosis, prognosis and even can be targeted in personalized therapy. Hence, these bio-molecules will get exposed frequently, and their new cellular role will emerge, soon. This review outlines the current trend, limitations, and future potential of circRNA in cancer research.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125495057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Immunohistochemistry as an important tool for exploring the insights of various aspects of gastro-intestinal tract 免疫组织化学作为一种重要的工具来探索胃肠道的各个方面
The Applied Biology & Chemistry Journal Pub Date : 2020-12-25 DOI: 10.52679/TABCJ.2020.0009
R. Shukla, N. V. A. Rao
{"title":"Immunohistochemistry as an important tool for exploring the insights of various aspects of gastro-intestinal tract","authors":"R. Shukla, N. V. A. Rao","doi":"10.52679/TABCJ.2020.0009","DOIUrl":"https://doi.org/10.52679/TABCJ.2020.0009","url":null,"abstract":"The concepts in immunology and techniques in histology have come together in a novel way to create a pioneering discipline known as ImmunoHistoChemistry (IHC), to discover new ways in detecting cell and tissue antigens related to amino acids, proteins and infectious agents by using labeled antibodies. These amalgamation techniques are applied in the disciplines of endocrinology, entero-biology, neurobiology, pathology, tumor biology and pharmaceutical research as a diagnostic tool. The simultaneous advancements in the field of imaging techniques further assisted and widened the application of IHC in molecular studies, thereby facilitating the development of novel therapeutic strategies. This paper attempts to discuss the different aspects of gastro-intestinal tract in relation to its cellular diversity, cellular differentiation, physiology and pathology, through the application of IHC methods.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115052433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Relationship between human genetics and susceptibility to COVID-19 infection 人类遗传学与COVID-19感染易感性的关系
The Applied Biology & Chemistry Journal Pub Date : 2020-12-15 DOI: 10.52679/TABCJ.2020.0007
Preetinder Kaur, P. Kaur
{"title":"Relationship between human genetics and susceptibility to COVID-19 infection","authors":"Preetinder Kaur, P. Kaur","doi":"10.52679/TABCJ.2020.0007","DOIUrl":"https://doi.org/10.52679/TABCJ.2020.0007","url":null,"abstract":"Recent studies have shown that the progression of SARS-CoV-2 is associated with human genetic factors. Many patients have shown diverse clinical symptoms based on their age, sex, ABO blood type, underlying medical conditions, HLA system, and viral variants found in their samples. SARS-CoV-2 is considered more transmissible with higher morbidity rates as compared to the previous SARS outbreak. Several case studies have supported the direct relation of cardiovascular and pulmonary fatalities related to ACE2 polymorphisms during COVID-19 infection. SARS-CoV-2 mutants and human genome polymorphisms are vital predictive markers in finding a cure for this pandemic. This review focuses on some of the critical genetic factors of the host that affect the array of immunological responses as a result of COVID-19 infection among individuals.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128661440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A journal of new and advance biology 最新的和先进的生物学杂志
The Applied Biology & Chemistry Journal Pub Date : 2020-09-13 DOI: 10.52679/TABCJ.2020.0001
Navpreet Kaur, N. Rao
{"title":"A journal of new and advance biology","authors":"Navpreet Kaur, N. Rao","doi":"10.52679/TABCJ.2020.0001","DOIUrl":"https://doi.org/10.52679/TABCJ.2020.0001","url":null,"abstract":"The aim of The Applied Biology & Chemistry Journal (TABCJ) is to be a focus for communication in research on the application of biological, biomedical and chemical sciences for studying, analyzing and understanding biological problems. Although the primary purpose of TABCJ is to offer a journal where cutting-edge research of broad interest may be published rapidly, without compromising on the quality, the additional features including avant-garde reviews will extend the scope of the journal by continuing its role beyond the reporting of research results.","PeriodicalId":424992,"journal":{"name":"The Applied Biology & Chemistry Journal","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124786658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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