veterinary medical journal Giza最新文献

{"title":"Efficient Stable Expression of Nuclear H5N1 Avian Influenza Virus HA2 Transgene in Chlamydomonus reinhardtii","authors":"M. Bayoumi, M. Samir, M. Elgamal, H. Amer, Ausama Yousif","doi":"10.21608/VMJG.2017.7641","DOIUrl":"https://doi.org/10.21608/VMJG.2017.7641","url":null,"abstract":"Green biotechnology is the future of biopharmaceuticals production. The use of algae to produce biopharmaceuticals is of interest in vaccine-based control programs because of cost and environmental safety considerations. An attempt was made to express avian influenza virus (AIV) immunogens in algae because the virus is a serious economic, veterinary, and public health threat. A commercial system was modified to allow expression of H5N1 AIV hemagglutinin subunit 2 (HA2) in the microalga Chlamydomonas reinhardtii (C. reinhardtii). Codon-optimized AIV H5N1 HA2 (coHA2) sequence was synthesized and cloned into the transfer vector pChlamy_3/D-TOPO ® . Cloned coHA2 sequence was then transformed in C. reinhardtii strain cc-125 by electroporation. Proper nuclear integration was confirmed in 16% of screened transformants selectively amplified in Hygromycin-containing TAP media. coHA2 mRNA transcription was confirmed using RT-PCR. AIV HA2 expression was confirmed using western blot analysis utilizing AIV polyclonal chicken antisera. Expressing transformants were maintained on Hygromycin-containing TAP agar for 26 weeks (15 subcultures). Expressing transformants maintained cell shape, motility and, growth characteristics similar to non-transformed C. reinhardtii cc-125. A coHA2-C terminus GFP was used to visualize HA2 expression in vivo using confocal microscopy. Background-normalized GFP-specific fluorescence of transformants was 15 % of the total cellular fluorescence. Fluorescence in GFP channels 508, 518, 528 and 538 nm was 3.9% of the total cellular fluorescence of non-transformed algae. Taken together, results indicate efficient stable expression of the AIV HA2 transgene and, warrant further investigation into immunogenic potential of the algae-expressed HA2.","PeriodicalId":421382,"journal":{"name":"veterinary medical journal Giza","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122037937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Importance of Circulating anti-Müllerian Hormone as a Predictor of Superovulatory Response in Dairy Holstein Cows","authors":"Ebtihal Ibrahim, D. A. El-Moneim, M. Mahmoud","doi":"10.21608/vmjg.2017.7639","DOIUrl":"https://doi.org/10.21608/vmjg.2017.7639","url":null,"abstract":"Plasma anti-Müllerian hormone concentration (AMH) in cows is a useful endocrine marker for ovarian response to gonadotropin superstimulation. Here, we investigated the possible relationship between AMH concentration measured in plasma and superovulatory response of embryo donor dairy Holstein cow aiming to predict their response in earlier period before beginning of superovulatory treatment protocol. So, for this reason, 33 cows were undergo superovulatory gonadotropin treatment and blood samples were collected at three phases (P0 randomly before treatment, PE on day of estrus, PF on day of flushing for embryos collection). This study revealed that AMH concentration were significantly increased in the high responded cows in all 3 phases of study than the low responded ones. Also, the study reported 3 optimal cut-off points 57.6, 192.1 and 114.2pg/ml in the P0, PE and PF phases respectively which could be used for early prediction of cow superovulatory responses. In conclusion, circulating AMH concentration was strongly associated with superovulation response and its evaluation could be used to identify embryo donor cows with greater response to superovulation programs. Also, the optimal cut-off point 57.6pg/ml could be used randomly to select the best donors (Key wards: Superovulation, anti-Müllerian Hormone (AMH), dairy cows) Introduction Recent advances in bovine biotechnology, such as commercially available genomic testing, have allowed for the identification of animals with superior genetics. Genetic selection and reproductive efficiency are key factors for the success of the dairy and beef industries. In cattle, Multiple Ovulation and Embryo Transfer (MOET) programs have become a large international business. More accurate identification of cows with greater embryo production potentials could allow for more efficient production of in vitro and in vivo bovine embryos for cows with superior genetics (Rico et al., 2009). However, cost efficient propagation of these superior genetics has been hampered by high variability between animals in response to embryo production techniques such as superovulation (Souza et al., 2015). Despite improvements in superovulatory treatments, ovarian responsiveness to gonadotropins remains highly variable between individuals and difficult to predict. It is well established that the major source of variability is the status of ovarian follicles at the time of initiation of FSH treatment (Rico et al., 2009). Presently, some clinical studies thrown its light on anti-Müllerian Hormone (AMH) describing it as the best endocrine marker of ovarian follicular reserve, largely replacing other serum markers such as inhibin B, estradiol, basal FSH and LH (Fanchin et al.,2003; Broekmans et al., 2006; Toner and Seifer, 2013). AntiMϋllerian Hormone is also the best predictive marker of the ovarian response to stimulatory treatment as defined by the number of oocytes retrieved In Assisted Reproductive Technology (ART) (El-Gindy e","PeriodicalId":421382,"journal":{"name":"veterinary medical journal Giza","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134045197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of cefquinome in camels.","authors":"A. Al-Taher","doi":"10.3923/JAVAA.2010.848.852","DOIUrl":"https://doi.org/10.3923/JAVAA.2010.848.852","url":null,"abstract":"","PeriodicalId":421382,"journal":{"name":"veterinary medical journal Giza","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116217479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Dietary Zinc Oxide Nanoparticles on Zootechnical Performance and Biometric Indices of Growing New Zealand White Rabbits","authors":"E. Massoud, Walaa Goda, H. M. Teleb, R. El-Banna","doi":"10.21608/VMJG.2021.158008","DOIUrl":"https://doi.org/10.21608/VMJG.2021.158008","url":null,"abstract":"This study was designed to compare the effects of dietary conventional inorganic Zinc oxide (ZnO) and nano-zinc oxide (ZnO-NPs) on the zootechnical performance, selected blood serum indices, antioxidant status, general health and production of rabbit meat enriched with zinc enriched with nano-zinc in growing New Zealand White rabbits (NZW). A total of 481 newly-born rabbits produced from two natural successive mating between 36 primiparous (NZW) Rabbit does and 12 bucks by the intensive breeding system were utilized throughout this study. Rabbits does and bucks were randomly distributed into four equal groups, the control positive group G1 was fed the basal diet supplemented with premix contain 50 ppm inorganic zinc in the form of ZnO; while the control negative G2 was fed on a basal diet supplemented with Zinc Free premix, meanwhile, G3 was fed on the basal diet supplemented with premix contain 25 ppm nano zinc as zinc oxide nanoparticles, however, G4 was fed on the basal diet supplemented with 50 ppm nano zinc as zinc oxide nanoparticles in the premix, Litters after weaning continued on the same diet like their dams till the age of selling at 62 days old. The results showed that the growing rabbits in (G4) supplemented with 50 mg ZnO-NPs/kg diet surpassing significance (P≤ 0.05) in all groups and achieved the best result regarding (birth weight(g), weaning weight(g), sealing weight (g) moreover lower Pre-weaning mortality %, and Post-weaning mortality%). Also, G4 showed significant improvement in FCR in comparison with other groups. Furthermore, showed highest significant increase in total protein and globulin, HDL, SOD, GSH, Catalase activity, and zinc concentration in serum, liver, and thigh tissue and also showed the highest significant decrease in AG ratio, creatinine, urea, uric acid, cholesterol, Triglyceride, LDL, VLDL, risk factor, MDA and NO level in G4 at 62 day age old, compared to other groups, while there was no significant difference in albumin level between all the experimental groups. In conclusion, supplementation of 50 mg ZnO-Npskg diet in the premix to the diet of growing NZW rabbit maximize the productive performance and improve FCR without any adverse effect.","PeriodicalId":421382,"journal":{"name":"veterinary medical journal Giza","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125519598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Heat Shock and Electroporation Transformation of Canine Homlogue of HER2 Gene Cloned in a PUC57 Plasmid Construct","authors":"K. Shokry, Rehab El Helw, M. A. Ghoneim, Sa. Selim","doi":"10.21608/VMJG.2021.158007","DOIUrl":"https://doi.org/10.21608/VMJG.2021.158007","url":null,"abstract":"","PeriodicalId":421382,"journal":{"name":"veterinary medical journal Giza","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114315816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}