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Nano-Gold Based ELISA versus traditional ELISA for early detection of Trichinella spiralis crude excretory antigen in experimentally infected mice 纳米金酶联免疫吸附试验与传统酶联免疫吸附试验早期检测旋毛虫粗分泌物抗原的比较
IF 0.4
Parasitologists United Journal Pub Date : 2023-03-11 DOI: 10.21608/puj.2023.180498.1198
S. El-Refai, N. Nassef, Manal El-Melegy, E. Elsayed, E. Beshay
{"title":"Nano-Gold Based ELISA versus traditional ELISA for early detection of Trichinella spiralis crude excretory antigen in experimentally infected mice","authors":"S. El-Refai, N. Nassef, Manal El-Melegy, E. Elsayed, E. Beshay","doi":"10.21608/puj.2023.180498.1198","DOIUrl":"https://doi.org/10.21608/puj.2023.180498.1198","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46227202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cholagogue Additive Effect of Ursodeoxycholic acid to Praziquantel on murine Schistosomiasis mansoni: Parasitological and Histopathological studies 熊去氧胆酸与吡喹酮对小鼠曼氏血吸虫病的加性催胆作用:寄生虫学和组织病理学研究
IF 0.4
Parasitologists United Journal Pub Date : 2023-01-20 DOI: 10.21608/puj.2023.174365.1195
Shaimaa Mohamed, H. Abdelmaksoud, H. Sabry, Soheir S. Mahmoud, Wafaa Elkomy, T. Aboushousha, A. Ashkar
{"title":"Cholagogue Additive Effect of Ursodeoxycholic acid to Praziquantel on murine Schistosomiasis mansoni: Parasitological and Histopathological studies","authors":"Shaimaa Mohamed, H. Abdelmaksoud, H. Sabry, Soheir S. Mahmoud, Wafaa Elkomy, T. Aboushousha, A. Ashkar","doi":"10.21608/puj.2023.174365.1195","DOIUrl":"https://doi.org/10.21608/puj.2023.174365.1195","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46953646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The contribution of in-silico studies in parasitology: A multifaceted approach 计算机研究对寄生虫学的贡献:一个多方面的方法
IF 0.4
Parasitologists United Journal Pub Date : 2023-01-14 DOI: 10.21608/puj.2023.160249.1188
A. Ammar, N. Elleboudy, Shimaa S. Mohammed
{"title":"The contribution of in-silico studies in parasitology: A multifaceted approach","authors":"A. Ammar, N. Elleboudy, Shimaa S. Mohammed","doi":"10.21608/puj.2023.160249.1188","DOIUrl":"https://doi.org/10.21608/puj.2023.160249.1188","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44767520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunological evaluation of the prophylactic impact of soluble egg antigen (SEA) combined with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni 可溶性卵抗原（SEA）联合血吸虫肺抗原制剂（SLAP）对曼氏血吸虫病小鼠模型预防作用的免疫学评价
IF 0.4
Parasitologists United Journal Pub Date : 2022-12-27 DOI: 10.21608/puj.2022.174048.1193
A. Elkholy, Nagat H. Soliman, M. Elawady, R. Omar
{"title":"Immunological evaluation of the prophylactic impact of soluble egg antigen (SEA) combined with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni","authors":"A. Elkholy, Nagat H. Soliman, M. Elawady, R. Omar","doi":"10.21608/puj.2022.174048.1193","DOIUrl":"https://doi.org/10.21608/puj.2022.174048.1193","url":null,"abstract":"Background: Vaccination against schistosomiasis might be an excellent approach for its control and elimination. Despite the recent advances in identification of several potential vaccine candidates, no vaccine was validated for clinical trials. Objective: This research aims to evaluate the preventive effects of soluble egg antigen (SEA) in combination with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni . Material and Methods: In this study, 35 Swiss albino male mice (aged 6 to 8 weeks) were divided into five groups. Each group contained seven mice, with G1 serving as the normal control, G2 the infected control, G3 the infected and immunized by SEA, G4 the infected and immunized by SLAP, and G5 the infected and immunized by combination antigens (SLAP and SEA). All mice were infected two weeks after receiving their final dose of immunization. Seven weeks following infection, mice were decapitated in order to determine the impact of the injected antigens using determination of liver enzymes, IL10, TNF-α and schistosomal","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49086461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advances in identification of potential drug targets and development of novel drugs in protozoal diseases. Part IV. Apicomplexans and Plasmodium spp. 原生动物疾病潜在药物靶点的鉴定和新药开发的最新进展。第四部分Apicocomplians和疟原虫。
IF 0.4
Parasitologists United Journal Pub Date : 2022-12-27 DOI: 10.21608/puj.2022.171955.1192
S. Abaza
{"title":"Recent advances in identification of potential drug targets and development of novel drugs in protozoal diseases. Part IV. Apicomplexans and Plasmodium spp.","authors":"S. Abaza","doi":"10.21608/puj.2022.171955.1192","DOIUrl":"https://doi.org/10.21608/puj.2022.171955.1192","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48306322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Omics: Applications related to diagnosis, treatment, prevention and control of parasitic diseases. Part II. Helminths. 奥密克戎：与寄生虫病的诊断、治疗、预防和控制有关的应用。第二部分。蠕虫。
IF 0.4
Parasitologists United Journal Pub Date : 2022-12-27 DOI: 10.21608/puj.2022.177647.1197
Radwa G Diab, Salwa S Younis
{"title":"Omics: Applications related to diagnosis, treatment, prevention and control of parasitic diseases. Part II. Helminths.","authors":"Radwa G Diab, Salwa S Younis","doi":"10.21608/puj.2022.177647.1197","DOIUrl":"https://doi.org/10.21608/puj.2022.177647.1197","url":null,"abstract":"vaccine candidates identified against schistosomiasis are protein molecules expressed on the tegument surface or secreted by schistosomula such as","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49455274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE EFFECTS OF COMMON HOUSE FLY (MUSCA DOMESTICA) LARVAE-DERIVED SUBSTANCES ON WOUND HEALING IN MOUSE MODEL 家蝇幼虫衍生物质对小鼠创面愈合的影响
IF 0.4
Parasitologists United Journal Pub Date : 2022-12-24 DOI: 10.21608/puj.2022.169218.1190
D. Said, Hoda M Khalifa, Lamia El-Samad, M. Meheissen, Hala E. Diab, Radwa G Diab
{"title":"THE EFFECTS OF COMMON HOUSE FLY (MUSCA DOMESTICA) LARVAE-DERIVED SUBSTANCES ON WOUND HEALING IN MOUSE MODEL","authors":"D. Said, Hoda M Khalifa, Lamia El-Samad, M. Meheissen, Hala E. Diab, Radwa G Diab","doi":"10.21608/puj.2022.169218.1190","DOIUrl":"https://doi.org/10.21608/puj.2022.169218.1190","url":null,"abstract":"Background: Maggot debridement therapy is a therapeutic wound myiasis that depends mainly on L. sericata larva. Owing to its availability and cheap breeding, M. domestica was suggested as an alternative to Lucilia . Objective: The present study was designed to assess the role of M. domestica larvae-derived substances on wound healing in immunocompetent and immunosuppressed mice. Material and Methods: Chitosan and gut extracts, obtained from M. domestica larvae, were applied daily on skin wounds of Swiss albino mice. Mice were divided into two groups, control non-treated mice (I), and experimental treated mice (II). Each group included immunocompetent (a), and immunosuppressed (b) mice. The experimental subgroups were treated with either chitosan (1) or gut extracts (2). Wounds were assessed by macroscopic evaluation, wound contracture, histopathological, immunohistochemical and bacteriological parameters. Results: In control non treated mice hair growth was evident with normal underlying skin by the end of the experiment (4 weeks) except in immunosuppressed (Ib) subgroup. Significant reduction in wound size was detected on the 7 th day post wounding (PW) in the immunocompetent-chitosan-treated subgroup","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44512365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hormonal changes in Trypanosoma evansi-infected Rattus norvegicus: An approach to understand host-parasite interaction 伊氏锥虫感染褐家鼠的激素变化：一种了解宿主与寄生虫相互作用的方法
IF 0.4
Parasitologists United Journal Pub Date : 2022-12-01 DOI: 10.21608/puj.2022.174437.1196
R. Mohamed, A. Darwish, M. Mohamed
{"title":"Hormonal changes in Trypanosoma evansi-infected Rattus norvegicus: An approach to understand host-parasite interaction","authors":"R. Mohamed, A. Darwish, M. Mohamed","doi":"10.21608/puj.2022.174437.1196","DOIUrl":"https://doi.org/10.21608/puj.2022.174437.1196","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47557306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stem cells therapy research for the treatment of parasitic infections 干细胞治疗寄生虫感染的研究
IF 0.4
Parasitologists United Journal Pub Date : 2022-11-29 DOI: 10.21608/puj.2022.156355.1185
Aliaa M Elsawey, S. Etewa, N. Aboulfotouh
{"title":"Stem cells therapy research for the treatment of parasitic infections","authors":"Aliaa M Elsawey, S. Etewa, N. Aboulfotouh","doi":"10.21608/puj.2022.156355.1185","DOIUrl":"https://doi.org/10.21608/puj.2022.156355.1185","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45357853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Leishmania donovani promotes macrophages polarization towards M2 phenotype in vitro: A new approach to identify a new therapeutic target 杜氏利什曼原虫在体外促进巨噬细胞向M2表型极化：一种确定新治疗靶点的新方法
IF 0.4
Parasitologists United Journal Pub Date : 2022-11-22 DOI: 10.21608/puj.2022.155256.1182
Samar Habib
{"title":"Leishmania donovani promotes macrophages polarization towards M2 phenotype in vitro: A new approach to identify a new therapeutic target","authors":"Samar Habib","doi":"10.21608/puj.2022.155256.1182","DOIUrl":"https://doi.org/10.21608/puj.2022.155256.1182","url":null,"abstract":"Background: The immune response against L. donovani depends significantly on infected macrophages. Since Leishmania amastigotes deploy several immune suppressive mechanisms to escape host immune responses, macrophages polarize towards the classically activated macrophages (M1) or the alternatively activated macrophages (M2). The balance between both types is crucial in shaping the infection outcome. Objective: The aim of this study is to explore the macrophage polarization behavior in response to L. donovani infection, and to examine the differential expression of IL-10 and TNF-α by each phenotype. Material and Methods: Leishmania -infected phorbol 12-myristate 13-acetate (PMA)-treated human leukemia monocytic cell line (THP-1) was used as an in vitro model of Leishmania infection. Leishmania stationary phase promastigotes were used to infect the macrophages at different multiplicities of infections (MOIs) i.e., ratio of macrophages to stationary phase promastigotes at 1:1, 1:10, and 1:20; and time points of 24 and 48 h post infection (PI). While CD68, CD40, HLA-DR were used as markers for M1; CD68 and CD163 were used to characterize","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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