Türk Mikrobiyoloji Cemiyeti Dergisi最新文献

Effect of Salt and Temperature Stress Resistance on the Tolerance of Candida parapsilosis to Extreme Conditions 盐和温度抗逆性对假丝酵母极端条件耐受性的影响

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 2019-03-25 DOI: 10.5222/tmcd.2019.024

Engin Kaplan, M. Ilkit, S. Hoog

{"title":"Effect of Salt and Temperature Stress Resistance on the Tolerance of Candida parapsilosis to Extreme Conditions","authors":"Engin Kaplan, M. Ilkit, S. Hoog","doi":"10.5222/tmcd.2019.024","DOIUrl":"https://doi.org/10.5222/tmcd.2019.024","url":null,"abstract":"Objective: Currently, Candida parapsilosis is an emerging cause of non-C. albicansrelated invasive candidiasis worldwide. Three phenotypically close species, C. parapsilosis, C. orthopsilosis and C. metapsilosis are defined as “Candida parapsilosis group”. Candida parapsilosis is frequently found in human commensal flora, natural environments, and inexplicably, in extreme indoor environments. This study aimed to examine the tolerance characteristics of C. parapsilosis to similar conditions of domestic dishwashing and washing machines. Method: We investigated the thermo-, halo-, pH and cycloheximide tolerance of 21 reference strains of C. parapsilosis (n = 8), C. orthopsilosis (n = 7), and C. metapsilosis (n = 6). Results: All isolates grew at 40°C but showed no growth at temperatures at or above 42°C (45°C and 50°C). All C. parapsilosis, C.metapsilosis, and C. orthopsilosis strains tolerated 5-10% NaCl, pH 2.5-10, and 0.01% cycloheximide. However, the isolates did not grow at pH 12.5 or in the presence of 0.1% cycloheximide. Notably, all the examined isolates of the C. parapsilosis species group can show tolerance at least one of the following conditions as; 10% NaCl, pH 2.5-10, and 0.01% cycloheximide. The survival rates of Candida parapsilosis, C. metapsilosis and C. orthopsilosis isolates at 45°C were 50%,16.6%, and 14.2%, respectively. In addition, although 75% of C. parapsilosis isolates were tolerant to 17% NaCl, C. metapsilosis and C. orthopsilosis isolates were not tolerant to this condition. Conclusion: Thermophilic and halophilic properties of Candida parapsilosis are thought to play an important role in the tolerance mechanism against extreme conditions in household appliances.","PeriodicalId":413797,"journal":{"name":"Türk Mikrobiyoloji Cemiyeti Dergisi","volume":"217 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131460490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Microbiological Pollution Level of Lake Van and Lake Erçek Situated within the Borders of Van Province Van省境内Van湖和erperseek湖微生物污染水平测定

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.5222/TMCD.2021.02418

E. Aydın, M. Parlak, H. Güdücüoğlu, Y. Bayram

{"title":"Determination of Microbiological Pollution Level of Lake Van and Lake Erçek Situated within the Borders of Van Province","authors":"E. Aydın, M. Parlak, H. Güdücüoğlu, Y. Bayram","doi":"10.5222/TMCD.2021.02418","DOIUrl":"https://doi.org/10.5222/TMCD.2021.02418","url":null,"abstract":"Objective: Lake Van is one of the most important recreation areas of the region. Investigation of microbiological pollution of Lake Van is very important for public health. The aim of this study is to assess the microbiological pollution and determine water quality of the lakes Van and Erçek situated within Van Province, which are used for swimming and recreation to protect both human and environmental health. For this purpose, the presence of fecal coliform, total coliform and enterococci were investigated in samples taken from 29 different points of the lakes. Method: Between June and September 2015, 232 water samples were taken from predefined 29 points, every two weeks, and sent to the Public Health Laboratory of Van Province in 300-500 mL sterile plastic bottles in accordance with Bathing Water Quality Regulation (76/160 / EU: add-on clause 5. Membrane filtration method was used to analyze the samples for coliform bacilli and enterococci. Mandatory values according to bathing water quality regulation were taken as 2000 cfu/100 ml,10000 cfu/100 ml and 1000 cfu/100 ml for fecal coliform, total coliform and enterococcus, respectively. Results: In 196 (84%) of 232 water samples collected, no bacterial contamination was found, while 36 (16%) samples were found contaminated with fecal coliform, total coliform and Enterococcus species. At least one or more specimens of poor quality water were detected in 17 of 29 sampled beaches. Conclusion: It was envisaged to take precautions by restricting the use of the places where microbiological pollution was detected. In addition, regular analyzes are important for public health.","PeriodicalId":413797,"journal":{"name":"Türk Mikrobiyoloji Cemiyeti Dergisi","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121130945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation of Carbapenemase Genes in Carbapenem Resistant Enterobacterales Isolates: First KPC Report From Dokuz Eylul University Hospital 耐碳青霉烯类肠杆菌中碳青霉烯酶基因的研究:来自Dokuz Eylul大学医院的第一份KPC报告

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.5222/tmcd.2021.94899

Şeyda Şilan Okalın, Ayşe Nur Sarı Kaygısız, M. C. Ergon, İ. Öktem

{"title":"Investigation of Carbapenemase Genes in Carbapenem Resistant Enterobacterales Isolates: First KPC Report From Dokuz Eylul University Hospital","authors":"Şeyda Şilan Okalın, Ayşe Nur Sarı Kaygısız, M. C. Ergon, İ. Öktem","doi":"10.5222/tmcd.2021.94899","DOIUrl":"https://doi.org/10.5222/tmcd.2021.94899","url":null,"abstract":"Objective: In recent years, increasing carbapenem resistance of Enterobacterales bacteria limits treatment options, considerably. The main mechanism of this resistance is the production of carbapenemase enzymes. The aim of this study is to determine carbapenemase gene types in Enterobacterales isolates from our hospitalized patients and assess the clonal associations of the isolates with KPC gene. Method: A total of 48 clinical Enterobacterales isolates resistant to at least one carbapeneme and received between January 2019 and March 2019 were included in the study. Sample types were consisted of urine, blood, tracheal aspirate, wound and sputum. Of these isolates, three were Escherichia coli while 45 were Klebsiella pneumoniae. Types of carbapenemases were investigated by polymerase chain reaction, using specific primers for VIM, IMP, NDM, KPC and OXA-48 genes. PFGE was performed to determine the clonal associations between blaKPC positive K. pnemoniae isolates. Results: According to the results, blaOXA-48 (n=2) and blaKPC (n=1) were found to be present among E. coli isolates. Regarding 45 K. pneumoniae isolates; only blaOXA-48 and only blaNDM were present in 30 and two isolates, respectively. Seven K. pneumoniae isolates were found positive for both blaOXA-48 and blaNDM. Remaining K. pneumoniae isolates (n=6) harboured only blaKPC. None of the isolates were positive for blaIMP and blaVIM. PFGE analysis showed four isolates had the same pulsotype (A), while two had different pulsotypes (B-C). Conclusion: To our knowledge, this is the first report of KPC gene isolated in Dokuz Eylul University Hospital.","PeriodicalId":413797,"journal":{"name":"Türk Mikrobiyoloji Cemiyeti Dergisi","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127511291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Bacterial Meningitis Agents by Culture and PCR in Patients Diagnosed with Meningitis 培养和PCR检测脑膜炎患者细菌性脑膜炎药物

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.5222/tmcd.2019.191

S. Toprak, K. Can, R. Çalışkan, G. Şengöz, Z. Habip, Edip Tokuç, M. Demirci, Z. Taner, M. M. Torun, B. Kocazeybek, H. B. Tokman

{"title":"Determination of Bacterial Meningitis Agents by Culture and PCR in Patients Diagnosed with Meningitis","authors":"S. Toprak, K. Can, R. Çalışkan, G. Şengöz, Z. Habip, Edip Tokuç, M. Demirci, Z. Taner, M. M. Torun, B. Kocazeybek, H. B. Tokman","doi":"10.5222/tmcd.2019.191","DOIUrl":"https://doi.org/10.5222/tmcd.2019.191","url":null,"abstract":"Objective: We aimed to determine the community-acquired bacterial meningitis and nosocomial bacterial meningitis cases in patients diagnosed with meningitis in our hospital. At the same time we aimed to determine in these cases whether multiplex PCR was advantageous in the detection of Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae, Group B Streptococcus and Listeria monocytogenes compared to culture. Methods: Our study was performed with cerebrospinal fluid (CSF) samples of 100 adult patients admitted to Cerrahpaşa Medical Faculty between February 2012 and June 2013 and were diagnosed as meningitis. Samples were taken in two tubes. From the first tube, macroscopic examination of the CSF sample was performed, followed by inoculation, cell counting, Gram staining and direct antigen determination. The sample in the 2nd tube was inoculated in a blood culture flask (BD Bactec FX) and was incubated at 37°C. Bacteria were identified using standard clinical microbiology methods and further identification was made with API (BioMérieux, France) kits. Additionally, Seeplex Meningitis-B Ace Detection kit (Seegene Inc., Korea) was used for molecular detection. The presence of more than 200 leukocytes in the CSF samples and the predominance of PNL supported the diagnosis of meningitis. Results: Bacteria were detected in eight of 66 clear CSF samples (12.1%), in four of 12 xanthochromic CSF samples (30%) and in nine of 22 blurred CSF samples (40.9%). In CSF samples of cases with nosocomial meningitis, the most common bacteria were methicillin-resistant coagulase-negative staphylococci (n=6, 30%), and Klebsiella spp. (n=4, 20%). In one patient with community-acquired meningitis S. pneumoniae was isolated by multiplex PCR. Conclusion: Determination of bacteria causing community-acquired meningitis should not be based solely on the culture method, the advantages of PCR method should be utilized.","PeriodicalId":413797,"journal":{"name":"Türk Mikrobiyoloji Cemiyeti Dergisi","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116013989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Antimicrobial Susceptibility of Nosocomial Pseudomonas aeruginosa Isolates According to EUCAST and CLSI Breakpoints and Investigation of the Existance of Metallo Beta Lactamase by Phenotypic Methods 基于EUCAST和CLSI断点的医院内铜绿假单胞菌药敏比较及表型方法对金属β -内酰胺酶存在的调查

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.54453/tmcd.2022.07379

Sulhiye Aslan, G. Yenişehirli, Bahise Çağla Taşkın Dalgıç, A. Yenişehirli

引用次数: 0

Candida Vajinitinin Tedavisinde Otesekonazol (VT-1161): Güncel Durum

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.54453/tmcd.2022.36024

Ayşe Sultan Karakoyun, M. Sucu, Macit İlki̇t

{"title":"Candida Vajinitinin Tedavisinde Otesekonazol (VT-1161): Güncel Durum","authors":"Ayşe Sultan Karakoyun, M. Sucu, Macit İlki̇t","doi":"10.54453/tmcd.2022.36024","DOIUrl":"https://doi.org/10.54453/tmcd.2022.36024","url":null,"abstract":"Antifungal drugs used for the treatment of fungal infections have a high risk of drug-drug interactions and side effects (such as hepatotoxicity and cardiotoxicity). Therefore, the search for novel antifungal drugs continues. Azole antifungals are among the most preferred drug classes in the treatment of invasive and mucosal fungal infections. This group of drugs targets lanosterol 14α-demethylase, which is responsible for the synthesis of ergosterol in the structure of the fungal cell membrane. However, their use is limited, as they also interact with human cytochrome enzymes. Oteseconazole (VT-1161) is a new-generation antifungal drug class of tetrazole. It causes fewer drug-drug interactions and side effects, as it acts by selectively binding to the fungal cytochrome P450 51 (CYP51). Studies have also shown that VT-1161 is effective against fluconazole-resistant Candida albicans and Candida krusei, echinocandin-resistant Candida glabrata, dermatophytes, Cryptococcus, Rhizopus, and Coccidioides species. Candida vaginitis (CV) is an important problem with an increasing prevalence globally. VT-1161 is characterized by high oral bioavailability, a long plasma half-life (> 48 hours), and effective penetration into the vaginal mucosa. This review presents up-to-date information on the spectrum activity of oteseconazole and in vivo and in vitro data. Considering the limited treatment options for acute and recurrent CV, VT-1161 seems to be a safe and effective option.","PeriodicalId":413797,"journal":{"name":"Türk Mikrobiyoloji Cemiyeti Dergisi","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122178364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Architect® EBV Antibody Panel Results and EBV Diagnostic Algorithms Architect®EBV抗体检测结果和EBV诊断算法的评价

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.54453/tmcd.2022.46354

Ö. Appak, Abdurrahman Gülmez, A. Sayıner

引用次数: 0

Antibiotic resistance patterns of Enterococcus species isolated from cattle and cattle farmers in Denizli,Turkey 从土耳其德尼兹利的牛和养牛户中分离的肠球菌种类的抗生素耐药性模式

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.5222/TMCD.2019.098

E. Mete, İ. Kaleli

引用次数: 0

Bacteria That Cause Urinary System Infections and Antibiotic Resistance Rates 引起泌尿系统感染的细菌和抗生素耐药率

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.5222/tmcd.2021.82787

B. Keski̇n, Emel Çalışkan, Sare Kaya, Ezgi Köse, Idris Sahin

引用次数: 3

An Imported Malaria Case Associated With Pregnancy in Northern Cyprus 北塞浦路斯一例与妊娠有关的输入性疟疾病例

Türk Mikrobiyoloji Cemiyeti Dergisi Pub Date : 1900-01-01 DOI: 10.54453/tmcd.2023.05900

E. Güler, Ulaş Hürdoğanoğlu, Kaya Süer

引用次数: 0