{"title":"Phytochemicals Screening and Effectiveness of Free Radical Inhibitors of Garlic (Allium sativum L.) Ethanol Extract from Timor Island","authors":"Melania Priska, Natalia Peni, Ludovicus Carvallo","doi":"10.14710/BIOMA.21.1.72-77","DOIUrl":"https://doi.org/10.14710/BIOMA.21.1.72-77","url":null,"abstract":"The purpose of the study was to determine the content of secondary metabolites and the effectiveness of garlic (Allium sativum L.) ethanol extract from Timor island which has the potential to inhibit free radicals. The method used for testing secondary metabolites is phytochemical screening using color reagents. The effectiveness of free radical inhibitors from the ethanol extract of garlic (Allium sativum L.) from Timor island was carried out in two steps are: 1.) Determination of DPPH wavelength (λ) maximum and 2.) Measurement of antioxidant activity using the DPPH method. The results showed the ethanol extract of garlic (Allium sativum L.) from Timor Island contained secondary metabolites of flavonoids, phenols, and terpenoids. The ethanol extract of garlic (Allium sativum L.) from Timor Island also has strongest effectiveness in inhibiting free radicals, with the acquisition of IC50 values <50 ppm which is equal to 9,729 ppm","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131514046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioakumulasi Timbal (Pb) Pada Makroalga Padina australis Hauck Di Perairan Laut Kota Makassar, Sulawesi Selatan","authors":"Wahyu Supardi, Andika Puspito Nugroho","doi":"10.14710/BIOMA.21.1.9-15","DOIUrl":"https://doi.org/10.14710/BIOMA.21.1.9-15","url":null,"abstract":"Pb lead metal is one of marine pollution material that can accumulate on macroalga Padina australis. The purpose of this research is to know the accumulation of Pb on P.australis, the abundance, and to know the Pb content correlation with abundance on P. australis. Research conducted on February 2018 in Makassar marine waters. The results indicate that there is a difference of the metal content of Pb on P.australis in each station. The metal content of Pb on the station I was at the range 0,0964-0,1388 ppm, Station II 0,0496-0,1050 ppm, and Station III 0,0597-0,1035 ppm which has exceeded the waters standard limit set>0.008 ppm. The high average Pb concentration on the station I because the station I was closer to the mainland of potentially greater influx of Pb heavy metals on macroalgae. P. australis abundance on a station I 92 individuals, II 152 individuals, and III 319 individuals. The data analysis result shows the value of significant correlations 0.384>0.05 that there is no correlation between the Pb concentrations with abundance but the direction of correlation indicates negative (-0331) which means that if the Pb concentration rises then P. australis abundance will decrease.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134252852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laily Kurniawati, Endang Kusdiyantini, W. Wijanarka
{"title":"Pengaruh Variasi Suhu Dan Waktu Inkubasi Terhadap Aktivitas Enzim Selulase Dari Bakteri Serratia marcescens","authors":"Laily Kurniawati, Endang Kusdiyantini, W. Wijanarka","doi":"10.14710/bioma.23.1.33-42","DOIUrl":"https://doi.org/10.14710/bioma.23.1.33-42","url":null,"abstract":"Enzymes are biocatalysts in living cells when cells metabolize. All living organisms are produced enzymes, both humans, animals, plants and microorganisms. One of the bacteria that has the potential to produce cellulose (EC 3.2.1.4) enzymes is Serratia marcescens. These bacteria can be isolated from water, soil and digestive tract. This research aims to find out the types of enzymes produced by S. Marcescens, to examine the effect of temperature and incubation time on selected enzyme activity. The type of enzyme test was qualitatively determined by S. marcescens growth on the amylolytic, cellulolytic, pectinolytic and chitinolytic selective medium based on the clear zone. This research was used a Completely Randomized Design (CRD). The first factor was the incubation time (T) which were 4 hours (T4), 8 hours (T8) and 12 hours (T12). The second factor was the treatment of incubation temperature (S) which were 40oC (S1), 50oC (S2) and 60oC (S3). Each treatment was repeated in 3 times. The data were obtained then analyzed using Anova (α = 0.05). If it is significantly different, furthermore proceed with the T test (BNT). The results showed that S. marcescens qualitatively produced only clear zones in the cellulolytic medium of 5.1 mm. The ANOVA results showed that incubation temperature (S), the interaction between incubation time (T) and incubation temperature (S) were did not have effect on cellulase activity, whereas incubation time (T) gives a significant effect on cellulase activity were obtained at the incubation time for 12 hours (T12) with a value of 0.27 U / mL","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"42 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121015386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pengaruh Waktu Fermentasi Teh Kombucha Kadar 50% Terhadap Tebal Dinding Dan Diameter Lumen Arteria Koronaria Tikus Putih","authors":"Sri Isdadiyanto","doi":"10.14710/BIOMA.20.2.140-145","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.140-145","url":null,"abstract":"The objective of this study was to analyze the influences of 50% kombucha tea level on wall thickness and lumen diameter of coronary artery in rats. Sixteen male Rattus norvegicus rats, 2 months of age were used as experimental animals, with treatment kombucha tea that has been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. The research design used to a completely randomized with 4 treatment (for 4 weeks) and 4 replications, i.e., P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that has been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that has been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that has been fermented for 12 days in morning and afternoon. Variables measured were wall thickness and lumen diameter of coronary artery. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 10,0. The result showed that kombucha tea did not influences the wall thickness and lumen diameter of coronary artery in rats. Key words: Rattus norvegicus, 50% kombucha tea level, wall thickness, lumen diameter, coronary artery","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124347884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dzunnuroini Khanif Makhfudhoh, Jafron Wasiq Hidayat, Fuad Muhammad
{"title":"Struktur Komunitas Plankton sebagai Indikator Kualitas Perairan Budidaya di Tambak Lorok Semarang","authors":"Dzunnuroini Khanif Makhfudhoh, Jafron Wasiq Hidayat, Fuad Muhammad","doi":"10.14710/BIOMA.20.2.145-153","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.145-153","url":null,"abstract":"Plankton is organisms that had an important role in water as natural feed of fishes and as an indicator. This study aimed to identify water quality which was suitable for fish cultivation reviewed from physics-chemicals parameters of water and its saprobic status in Tambak Lorok Tanjung Mas Kota Semarang. Sampling were take based on 5 stations that could represented from plankton variety of condition water stability . Plankton samples were by using net plankton number 25, then preserved with alcohol 70% and formalin 4%. Variables of ponds water quality measured were temperature, salinity, dissolved oxygen, pH, turbidity, N concentration, and Pb content. Identification of planktons used SRC method under microscop. The result of plankton analysis in Tambak Lorok water showed 42 phytoplankton species, the commonly species found are Coscinodiscus centralis, Coscinodiscus granii, Gyrosigma sp., Navicula radiosa, Pandorina sp., and Melosira sp. Meanwhile, zooplankton found was 22 species with common species are Cyclops, Harpaticoida, Tricodesmium evythraeum, Brachinus plicatilis, and Lensia subtilis. Phytoplankton diversity index are 0.057-0.367 and equity index are 0.025-0.176. Zooplankton diversity index are 0.247-0.360 and equity index are 0.157-0.201. This indices showed that there were low level of species diversity and community stability, dominance since the species occurred. Measurement of water quality variables indicated that Tambak Lorok had been contaminated and needed a preliminary management before d be used as fish cultivation ponds, because the analysis result was not appropriate with Government water quality regulation. Based on the measurement, soprobic index categories on α/β-Mesosaprobic to Oligosaprobic phase and the degree of pollution is very mild to moderate. Keywords : Community Structure, plankton, Tambak Lorok, Tanjung Mas, Semarang ","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114434017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Riani Nurasri, Enny Yusuf Wachidah Yuniwarti, M. Djaelani
{"title":"Pengaruh Pemberian Virgin Coconut Oil (VCO) dan Olive Oil terhadap Mikroanatomi Ren Tikus Putih (Rattus norvegicus)","authors":"Riani Nurasri, Enny Yusuf Wachidah Yuniwarti, M. Djaelani","doi":"10.14710/BIOMA.20.2.133-139","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.133-139","url":null,"abstract":"Virgin Coconut Oil (VCO) and olive oil have been widely applied in the field of health. Saturated fatty acid content of VCO dominates the total fatty acid content of about 85%. Olive oil contains a lot of oleic acid, which is one type of unsaturated fatty acid (mono-unsaturated fatty acid). This study aimed to analyze the kidney microanatomy rat (Rattus norvegicus) after supplementation with VCO and olive oil in the feed. This study used 25 rats were divided into five treatment groups, P1 was the control group, P2 was a group with VCO 5 mL / kg feed, P3 was a group with VCO 10 mL / kg of feed, P4 was a group with olive oil 5 mL / kg feed and was a group with olive oil 10 mL / kg of feed. The results of ren weight and diameter rat glomerular analyzed using analysis of variance (ANOVA). Research design used was completely randomized design with five treatments and five replication. Results of studies on weight ren showed no significant difference between treatments P2, P3, P4 and P5 to the control (P1). The results of the glomerular diameter shows there is significantly difference between the diameter of the glomerulous treatment P2 and P3 with control (P1), while in treatment P4 and P5 are not significantly different from the control so that it can be concluded that the administration of the VCO and olive oil at various concentrations do not give rise to different responses to weight ren. Olive oil does not increases glomerular diameter white mice, while VCO at a dose of 5 mL / kg feed and 10 mL / kg of feed can increased glomerular diameter mice. Keywords: ren weight, glomerular diameter, Virgin Coconut Oil (VCO), olive oil.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121506315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metode Monitoring Resistensi Populasi Aedes aegypti Dengan Penetapan Konsentrasi Diagnostik","authors":"Vicka Kusuma Sari, Udi Tarwotjo, M. Hadi","doi":"10.14710/bioma.20.2.105-112","DOIUrl":"https://doi.org/10.14710/bioma.20.2.105-112","url":null,"abstract":"Aedes aegypti is the main vector of dengue fever disease. The most effective way to prevent dengue fever is cutting the spreading chain of dengue fever by controlling the vector with using conventional chemical insecticides. The used of insecticide intensively has became the main factor of resistance in Tembalang, then monitoring is needed to determine the change of A. aegypti susceptibility status against the insecticide. The aim of this research is to determine the sensitivity of A. aegypti population from five locations in Tembalang against pyrethroid synthetic insecticide, and to determine the validity of the diagnostic concentration as a method of monitoring resistance A. aegypti population in Tembalang. This research was conducted in Laboratory of Ecology and Biosystematics Department of Biology Faculty of Science and Mathematics, Diponegoro University Semarang. The procedure of this research were: larvae collection (A. aegypti), insect breeding test, sensitivity test including bioassay, determination of diagnostic concentration and validation. The result showed that the susceptibility level of five population was not significantly different since all populations are still susceptible, with the LC50 range between 0,0031-0,0043% and FR range between 1-1,39 (<4). The result of validation test of diagnostic concentration was 0,0038%, valid as a monitoring method of the resistance of A. aegypti population against pyrethroid synthetic insecticide in Tembalang because c2 value of the five population was lower than the c2 table (df = 1; α = 0,05) = 3,84. Keywords : A. aegypti, monitoring resistance, LC50, diagnostic concentration..","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126503448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Glikogen Otot Rangka Ayam Broiler (Gallus gallus) setelah Pemberian Teh Kombucha dalam Air Minum","authors":"Nasrul Fathoni, M. Djaelani, Sri Isdadiyanto","doi":"10.14710/BIOMA.20.2.78-85","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.78-85","url":null,"abstract":"Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. The role of kombucha tea as a growth promoter is to that improve the metabolic process in the digestion of broiler chickens, so that nutrients can and fulfilled optimally for growth and development. The aim of this research was to analyze the sceletal muscle glycogen glycogen (Gallus gallus) after treat with kombucha tea in drinking water. Kombucha tea used is the result of fermentation of green tea for 12 days. This research used DOC chickens (Day Old Chicken) as much as 20 randomly divided into 4 treatments with concentration ie 0%, 10%, 20%, and 40% kombucha tea in drinking water for 32 days. The variables measured were skeletal muscle glycogen levels, body weight, feed consumption and drink consumption. The data obtained were analyzed using ANOVA followed by Duncan Test with 95% confidence level using SPSS 17.0 software. The results of this study showed that kombucha tea on skeletal muscle glycogen and feed consumption showed no significant difference, while on body weight and drink consumption showed significantly different results. Based on the results of the study, it can be concluded that giving kombucha tea in drinking water to a concentration of 40% has not been able to affect the skeletal muscle glycogen in broiler chickens. Key words: Kombucha tea, broiler chicken, glycogen skeletal muscle","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133929505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Aini, Yulita Nurchayati, Sri Widodo Agung Suedy
{"title":"Pengaruh Perendaman Akar Bibit Bayam Merah (Alternanthera amoena Voss.) dalam Larutan Na2CuEDTA terhadap Pertumbuhan dan Kandungan Antosianin","authors":"N. Aini, Yulita Nurchayati, Sri Widodo Agung Suedy","doi":"10.14710/BIOMA.20.2.123-132","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.123-132","url":null,"abstract":"Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for growth and anthocyanin content of red spinach. This research conduct to Complete Random Design of single factor pattern with 5 treatments and 5 replications which are aquades, nutrient solution, nutrient solution+Na2CuEDTA 5 ppm, nutrient solution+Na2CuEDTA 10 ppm, nutrient solution+Na2CuEDTA 20 ppm. Sprouts red spinach that already has 4 leaves soaked in the treatment solution for 10 days, then the plants moved into a soil medium inside the pot for 20 days. The analyzing data which is used is ANOVA continuing with further testing of Duncan Multiple Range Test (DMRT) at the 95% significance level. The result of this research show that soaking of red spinach in Na2CuEDTA solution did not show any significant effect on growth. The growth of red spinach plant is more influenced by nutrient solution while the anthocyanin contents is influenced by nutrients in soil medium. Key words : Red Spinach, Copper, Anthocyanin, Na2CuEDTA","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131534624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andy Wicaksono, Fuad Muhammad, Jafron Wasiq Hidayat, Damang Suryanto
{"title":"Pengaruh Komposisi Azolla pinnata Pada Pakan Terhadap Pertumbuhan Ikan Bandeng (Chanos chanos Forsskal) di Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara","authors":"Andy Wicaksono, Fuad Muhammad, Jafron Wasiq Hidayat, Damang Suryanto","doi":"10.14710/BIOMA.20.2.113-122","DOIUrl":"https://doi.org/10.14710/BIOMA.20.2.113-122","url":null,"abstract":"Milkfish is one of the main commodities in the field of fishery and is known to be the most cultivated. One of the important’s factors to the success of milkfish cultivation is feed availability, both quality and quantity. Feed quality is an important aspect that must be fulfilled because of its relation to nutrients required for the fish growth. Azolla pinnata has a good potential that can be used as an additional ingredient for the fish growth because of its protein-rich content. This study is aimed to examine the effect of Azolla pinnata powder feed on growth of milkfish (length and body weight). This research was conducted in May – July 2017 at Aquatic Animal Health Management Laboratory, Center of Brackish Water Aquaculture of Jepara. The research design used is a Complete Randomized Design (CRD), using five treatments with different concentrations of Azolla pinnata powder, i.e. 0% as control, 10%, 20%, 30%, and 40%. Each treatments consist of three repeatitions. The parameters observed were milkfish growth (length and body weight) and dissolved oxygen (DO). Data were analyzed by Analysis of Variance (ANOVA) method. If the data showing significant differences it will be continued with Least Significance Different (LSD) test. The results showed that the administration of Azolla pinnata powder on the feed significantly influenced the growth (lenght and body weight) of milkfish. The most optimal and efficient feed composition for milkfish growth is done by feeding 40% of Azolla pinnata powder. The overall dissolved oxygen (DO) is still in suitable condition for the cultivation of milkfish. Key Words : Azolla pinnata, dissolved oxygen, feed nutrition, milkfish","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124048276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}