Pakistan Journal of Biotechnology最新文献

{"title":"PURIFICATION AND CHARACTERIZATION OF AMYLASE PRODUCED FROM PROBIOTIC LACTOBACILLUS PLANTARUM CS FOR INDUSTRIAL APPLICATIONS","authors":"U. Nwachukwu, U. George-Okafor, K. Mba-Omeje, Amara Chioma Ezeme-Nwafor, Ifeoma Agatha Onah, Ifeanyi Jude Victor Egbuji","doi":"10.34016/pjbt.2024.21.02.890","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.02.890","url":null,"abstract":"Previous studies have demonstrated that probiotic Lactobacillus plantarum CS was able to generate an appreciable amount of extracellular amylase, hence the need to purify and characterize it. The aim of the study was to purify and characterize crude amylase from probiotic Lactobacillus plantarum CS for its industrial applications Three purification steps including ammonium sulphate precipitation, ion exchange chromatography on carboxymethyl sephadex and gel filtration on Sephadex G-75 were utilized. The homogeneity of the purified enzyme was confirmed using sodium deodocyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The purified amylase was characterized on different parameters including substrates hydrolyses, pH and temperature activity and stability profiles. The general purification elution profile revealed two different peaks of amylase activities with outstanding one having a molecular weight of 59.7kDa. Its purification fold was 4.0 with specific activity of 16.44U/mg protein and enzyme yield of 3%.  Temperature optimal activity and stability was at 400C and 7.5 for pH activity and stability. Mangenese (Mn2+) (135.17%), tween 80 (128.30%) and some food condiments garlic, thyme, ginger, and tumeric) significantly (p> 0.05) enhanced amylase activity (≥262.40%). However, selenium (Se4+) and hydrogen peroxide (H2O2) were observed to have greatest inhibiting effect (≥30.9%) on the enzyme. Substrate hydrolysis profiles showed that the amylase hydrolyzed all the test starchy substrates with the highest hydrolytic potential on indigenous sweet potato starch (Km value/ Vmax of 1.33mg/ml/ 7.89ml). The rate of hydrolysis of other test substrates had yam> rice>cassava>corn with km values ≤ 4.0mg/ml and Vmax ≤ 25ml.  The obtained results gave an insight that amylase produced from Lactobacillus plantarum CS met with the possessed properties suitable for any industrial application especially in food","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"8 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140706264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FUNCTIONAL COMPONENTS AND MEDICINAL PROPERTIES FOODS","authors":"Shaista Soomro, Shahzor Gul Khaskheli, A. Soomro, A. A. Panhwar, A. A. Khaskheli, Sana Nawaz, Abdul Ghani Dars","doi":"10.34016/pjbt.2024.21.02.901","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.02.901","url":null,"abstract":"The famous phrase \"Let food be the medicine\" (attributed to Hippocrates) basis for both food science and nutraceuticals. Numerous bioactive substances can be found in food components, such as nutritional elements like proteins, carbs, vitamins, minerals, antioxidants, phenolics, and flavonoids, as well as primary and secondary metabolites. Functional foods have a greater importance for their health benefits above and beyond basic nutritional requirements. Demand for functional foods is rising as individuals become more conscious of how their diet affects their health. Prebiotics are short-chain carbohydrates that promotes the growth of healthy bacteria, are converted into fatty acids in the colon, and help the body absorb calcium and magnesium. Intestinal cancer risk is also reduced by them. However, animal products also include useful components such bioactive peptides, conjugated linolenic acid, long-chain omega-3, -6, and -9 polyunsaturated fatty acids, probiotics, and prebiotics. The review seeks to provide readers with a comprehensive perception of the potential applications of the functional food components present in food extracts. Furthermore, Research is necessary to develop novel functional foods, with exact health benefits from understanding these foods and functional elements in food play a vital role in preserving good health in the future.","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"56 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140710879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INDUCED TOXIC EFFECTS OF CHLORPYRIFOS ON HEMATOLOGICAL INDICES IN PIGEONS (COLUMBA LIVIA DOMESTICA)","authors":"S. Memon, N. Memon, N. Birmani","doi":"10.34016/pjbt.2024.21.02.918","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.02.918","url":null,"abstract":"Agrochemicals are used extensively in agriculture sector across the world; however, they may be eco-toxicological hazards and disrupt the non-target living beings, including birds. Hence, the present study was carried out to evaluate the toxic effects of chlorpyrifos (CPF) insecticide on hematological parameters in pigeons. A total of 120 adult pigeons (Columba livia domestica) were used in the study and kept in wooden cages, and all pigeons received the same quantity of (22%) crude protein and water during trials. Pigeons were acclimatized for 15 days and divided into four equal (A, B, C and D) groups, each consisting of 30 birds. Test groups (B-D) were exposed to CPF with oral doses of “1.3 mg, 1.6 mg, and 2.1 mg/kg body weight/day” respectively for twelve successive weeks, whereas (group A) was assigned as a controlled group. At the end of the trials, blood samples from exposed and controlled group birds were obtained from brachial and collected with anticoagulant “K3-EDTA” vacutainer tubes for hematological assessments. Following the 84-day exposure period, notable statistically significant (p < 0.05) reduced levels of hemoglobin (Hb) (13.85 g/dl), (12.2 g/dl) and (10.50 g/dl) respectively, in birds of groups B, C and D and significant (p < 0.05) reduced level of total erythrocytes count (TEC) and packed cell volume (PCV%) was noted when compared with the control. There was a substantial significant (p < 0.05) increase in total leucocyte count (TLC) and total platelet count observed in comparison to the birds of the control group. The results of the present study revealed that alterations in hematological indices exhibited by chlorpyrifos insecticide. This research demonstrated how the indiscriminate use of pesticides damages health and developmental problems in bird ecosystems, and eventually, humans may be in danger for their health problems","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE EFFICACY OF NEEM OIL DERIVATIVES WITH IMIDACLOPRID AGAINST OKRA JASSID, AMRASCA BIGUTTULA (ISHIDA)","authors":"B. Mal, W. M. Mangrio, H. A. Sahito, Aqeel A. Siddiqui, S. Khuhro, F. I. Sahito","doi":"10.34016/pjbt.2024.21.02.915","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.02.915","url":null,"abstract":"The present research study was conducted at the experimental field condition of Pakistan Agricultural Research Council, Southern Zone Agricultural Research Centre, University of Karachi, to compare the field performance of different neem oil derivatives doses with Imidacloprid, sprayed on okra crop against the jassid population. The experiments were carried out RCBD with the application of four treatments, including three different concentrations of neem oils at (2%, 1.5%, and 1%) with the comparison of Imidacloprid pesticide and control. Neem oil was selected as a natural pesticide against jassid population reduction on okra crop. All the treatments were sprayed twice and data were recorded before 24h of pre-treatment and post-treatment at 48h, 72h, and 144h, respectively. The overall maximum reduction % after the application of Imidacloprid against the jassid population was calculated (61.51%) in 1st spray and (58.25%) 2nd spray, followed by (43.01%), (39.75%) at 2%, consequent to (32.50%), (29.75%), at 1.5% and (25.55%), (24.52%) at 1%, respectively. The maximum efficacy in neem oil concentration was recorded at 2% followed by 1.5%, and 1% but all neem oil concentrations including Imidacloprid observed effective from the 1st day up to the 6th post-spray days. All neem oil concentrations after 24h, 48h, 72h, and 144h of post-spray found significantly different at (P< 0.05). The application of neem oil, 2% caused the phytotoxicity reduction in okra plants. Thus, it is recommended that the bio-pesticide should be applied at 1.5% neem oil concentration against the jassid pest population.","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"137 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROXIMATE COMPOSITION, AMINO ACID PROFILING, AND SAFETY ASSESSMENT OF PROCESSED NATURAL VEGETATION OF THARPARKAR","authors":"Nida Shaikh, A. Soomro, T. Miano, O. M. Tarar","doi":"10.34016/pjbt.2024.21.01.899","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.899","url":null,"abstract":"Some processing techniques tend to advance the overall nutritional quality of food commodities and thus may contribute to counter malnutrition in regions like Tharparkar. For scanning the efficacy of some selected processing treatments, the natural vegetation of Tharparkar (NVT) i.e., mung beans (MB), sesame seeds (SS), and pearl millet (PM), were sprouted, roasted, and blanched, respectively, and milled to flour after thermal drying.  All fresh and processed NVT, i.e., sprouted mung bean flour (SMBF), roasted sesame seed powder (RSSP), and blanched pearl millet flour (BPMF), were analyzed for proximate composition, profiling of amino acids (AAs), and safety assessment. The results demonstrated statistically different (P<0.05) mean values of fresh and processed NVT for most of the analyzed parameters. It was found that processing significantly increased (P<0.05) ash, protein, fat, and fiber content whereas significantly decreased (P<0.05) phytic and oxalic acid in the NVT. In addition, some essential AAs significantly (P<0.05) enhanced in SMBF i.e., histidine (0.45g/100g), isoleucine (0.59g/100g), lysine (0.71g/100), methionine (0.23g/100g), phenylalanine (1.54g/100g), and valine (0.32g/100g); RSSP i.e., isoleucine (1.63g/100g), leucine (4.57g/100g), lysine (0.75g/100g), phenylalanine (3.14g/100g), tryptophan (0.81g/100g), and tyrosine (2.15g/100g); and in BPMF i.e., threonine (0.55g/100g), isoleucine (0.28g/100g), leucine (1.70g/100g), methionine (0.21g/100), tryptophan (0.11g/100g), and valine(0.17g/100g). Among non-essential AAs, the alanine and glycine remained significantly higher (P<0.05) in all processed NVT i.e., 1.56, 1.29, and1.43g/100g alanine in SMBF, RSSP, and BPMF, respectively. However, glycine remained at 0.56, 0.59, and 0.23g/100g in SMBF, RSSP, and BPMF, respectively. It is therefore concluded that processing techniques exerted a significant role in enhancing nutritional profile while mitigating the antinutrient load from the NVT. Consequently, it is recommended that SMBF, RSSP, and BPMF be utilized for developing various food products to counter malnutrition in regions like Tharparkar.","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140735599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SENSORY EVALUATION OF THE POTATO STARCH PUDDING: AS AN AFFORDABLE WAY OF ESSENTIAL NUTRIENTS FOR HUMAN CONSUMPTION","authors":"Elaf Shaikh, Tahira Bano, Alayesha Farooq, Amer Nazir, Hajrah Ilyas, Abdul Sabeer, Ahmed Hassan, Namra Aftab, Syeda Alishba, Naimatullah Koondhar, Mumtaz Ali Sahito","doi":"10.34016/pjbt.2024.21.01.878","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.878","url":null,"abstract":"The purpose of this study was to examine the nutritional value and energy content of potato pudding as an affordable way to get important nutrients such proteins, vitamins, and other energy components. Potatoes are a commonly available and reasonably priced staple item. Using them in pudding form presents an interesting way to feed people on a budget. This essay looks at the ingredients, health advantages, and possibilities as a high-energy food source of potato pudding. The objective of this work was to develop and characterize a new pudding recipe using potato flour as the main ingredient. The recipe was formulated by varying the ratios of potato flour and milk powder and by adjusting the sugar levels. The resulting puddings were evaluated for their texture, nutritional composition and sensory characteristics. The findings showed that the potato flour and milk powder- based pudding had a creamy texture with a smooth mouthfeel. The sensory assessment revealed a non- significant (p < 0.05) difference between potato pudding and control, suggesting that potato pudding formulations have a high potential to substitute potato starch.","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"8 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140235419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHO-YIELD RESPONSE AND QUALITY CHARACTERIZATION OF BOTTLE GOURD (LAGENARIA SICERARIA) VARIETIES TO NITROGEN FERTILIZATION","authors":"Muhzzam Anwar, Rizwan Wahid, N. A. Wahocho, Ali Ahmed Mugheri, Zahid Hussain, Khuda Dost, Safeer Ahmed, Kaleemullah Kakar, Jahanzaib, Naimatullah Koondhar","doi":"10.34016/pjbt.2024.21.01.895","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.895","url":null,"abstract":"The morpho-yield and quality traits of bottle gourd varieties were tested in response to nitrogen (N) nutrition during the year 2020 at the horticulture research area of Sindh Agriculture University Tandojam. The study was performed by applying Randomized complete block design (RCBD) with three replications. The two varieties Anmol and Long green were tested by applying six N rates that included (0 Kg N, (control), 50, 75 ,100, 125 and 150 Kg N ha-1). Both the varieties exhibited positive response to nitrogen fertilization. The maximum vine length, branches vine-1 were noted in plants, fertilized with increasing N rates (150 and 125 kg ha-1). In contrast shorter plants with less branches were noted when they were not fertilized with N nutrition.). The N nutrition also had a positive effect on fruit traits. The maximum fruit number with greater length and weight was recorded in plants supplied with increasing N fertilization. The N fertilization had beneficial influence on plant quality characteristics. The maximum pH, TSS, moisture and ash content in plants were observed, nourished with increasing N supply. The varietal comparison indicates that fruits of \"Long green\" had lower pH (5.88 vs. 6.0), higher in TSS (5.39 vs. 4.28%), moisture (67.74 vs. 55.52%), ash contents (19.96 vs. 19.01%), fruit yield (18178 vs. 17123 kg ha-1) than variety “Anmol”. In general, vines fertilized at 150 kg ha-1 N performed better than those fertilized at lower N levels for entire studied attributes, and \"Long green\" did better than \"Anmol\" among the evaluated varieties. The data clearly indicated severe soil N deficiency; and with each increment in applied N, the crop response was positive and significant (p<0.05). Hence, the farmers are suggested that “Long Green” bottle gourd variety may preferably be adapted with N at 150 kg ha-1 due to intense performance under agro-ecological conditions of Tandojam Sindh, Pakistan","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"164 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140235486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FIRST REPORT OF CURVULARIA LUNATA AND ALTERNARIA ALTERNATA CAUSING LEAF BLIGHTS ON TOMATOES IN SINDH, PAKISTAN","authors":"Qurban Ali Magsi, M. A. Saand, Muhammad Sajjad Kalwar, Sahib Ghanghro Sahib Ghanghro, Gul Hassan Shaikh Shaikh, Shoukat Ali Soomro Soomro, Ghulam Abid Dharejo Dharejo, Maria Khan Pathan Pathan, Ghulam Mustafa Bhatti Bhatti, Naimatullah Koondhar","doi":"10.34016/pjbt.2024.21.01.900","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.900","url":null,"abstract":"The tomato is an economically important crop that grows worldwide. The numerous fungal pathogens attacking tomato crops and causing severe diseases lead to yield loss. A few studies conducted regarding the identification of tomato fungal pathogens in Pakistan. However, no report was found to identify the fungal pathogens of tomato crops grown in Sindh. This study aimed to isolate, identify and characterize the tomato fungal pathogens in District Kamber Shahdadkot. Subsequently, thirty diseased tomato plant samples (leaves & stems) were collected from distinct villages of Taluka Kamber District Kamber Shahdadkot. These samples were collected in polythene bags and marked with symbols like S1, S2 and so on (S=Sample). All samples were brought into Botany Department lab and CBC Shah Abdul Latif University Khairpur. PDA media was prepared, sterilized and poured into petri plates. Prior to the inoculation, pieces from samples were soaked in a 1% solution of sodium hypochlorite (NaOCl) for a minute. The petri plates were inoculated separately and incubated at natural room temperature (260C to 280C) for arising and growth. Three days later, the inoculation site manifests signs and symptoms of fungal development.  Within a week fungus reached at preferable growth and spores were formed. Two slides from each petriplate were prepared i. e. one from the center of the cultures and other from the margins of the cultures. In order to finish this application, slide-sized scotch tape was cut up and immersed on the surface of cultures gently. The off cuts of the scotch tap introduced the fungal materials (mycelia and spores), which were pressed with love to the glass slides and observed. The fungi identification was made by using applications of morphological characteristics like morphology of the cultures, nature, and type of the mycelium, the shape of spores, and the color of the spores. Following these characters two fungal species were identified i. e. Curvularia lunata and Alternaria alternata","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"91 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140236184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFICACY OF ESSENTIAL OILS AGAINST Penecillium sp. CAUSING MUSHROOM MOLD UNDER IN VITRO CONDITION","authors":"Gohar F. Khan, Syed Zulfiqar Ali, Muhammad Waris, Abdul Qadir, Basher Ahmed, Muhammad Nadeem Sadiq, Muhammad Amin","doi":"10.34016/pjbt.2024.21.01.902","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.902","url":null,"abstract":"Mushrooms are nutritive and medicinal foods; Oyster and button are the very important mushrooms being grown at various farms, localities etc. Varieties of fungal contaminants limit the mycelium growth of the button mushroom as well as others in the substrate and affecting its yield. In this study, it was determined the Penecillium sp is the major mold affecting the yield of the Oyster mushroom. Furthermore In-Vitro experiment was carried out for management of mushroom against Penecillium sp. Essential oils viz., cinnamon oil, coconut oil, Neem oil and rose oil were used at various concentrations (4%, 8%, 12% and 16%) against Penecillium sp. All the essential oils showed impressive results, among all treatments Neem oil showed high reduction of colony growth of Penecillium sp. followed by coconut oil, Rose oil and Cinnamon oil. All the data was statically analyzed.","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"1 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140254596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TREATMENT FOR MALARIA PATIENTS IN PAKISTAN AND THE PREDOMINANCE OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PD) DEFICIENCY","authors":"Syed Waqad Ali, Marium Raziq, Muhammad Muzammil Khan, Sania Tanvir, Syed Jamal Haider Zaidi, S. Syed, Bullo Saifullah, Shahzad Nasim","doi":"10.34016/pjbt.2024.21.01.866","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.866","url":null,"abstract":"Even though it predisposes carriers to hemolysis, glucose-6-phosphate dehydrogenase (G6PD) deficiency is linked with malaria endemicity. This fact supports the malaria prevention theory. The objective of this paper to determine whether and how much there is a protective relationship between malaria and G6PD deficiency. Twelve databases were searched for studies describing any G6PD connection in malaria patients. 38 of the 50 included papers qualified for the review. Results indicated that there was no harmful association between G6PD deficiency and uncomplicated falciparum malaria in Even though it puts carriers at risk for hemolysis, glucose-6-phosphate dehydrogenase (G6PD) deficiency is widespread in areas of Pakistan where malaria is also prevalent. This data supports the malaria protection hypothesis. Pakistan's annual malaria burden is estimated to be 1.5 million cases. The government needs to execute a successful malaria control and eradication program, given the prevailing circumstances. Destroying Plasmodium falciparum gametocytes and eradicating Plasmodium vivax hypnozoite reservoirs are possible with primaquine. However, when using this medication, those who lack the enzyme glucose-6-phosphate (G6PD) experience hemolysis. The distribution of malaria and G6PD deficiency in Pakistan must be mapped to create an effective medication to suppress the disease. No significant reports of G6PD deficiency (G6PDd) in malaria patients have come from Pakistan. This review article seeks to establish the existence and magnitude of a protective connection between malaria and G6PD deficiency","PeriodicalId":411068,"journal":{"name":"Pakistan Journal of Biotechnology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140252397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}