Medical Perspectives-Medicni Perspektivi最新文献

{"title":"N-arylsulfonyl-2-aroylamino-1,4-quinone imines and their hydrogenated analogues: prediction of toxicity and prospects for use as diuretics","authors":"K. Sokolova, O. Podpletnia, S.O. Konovalova, A. Avdeenko, O. Komarovska-Porokhnyavets, V. Lubеnets, S. I. Kovalenko","doi":"10.26641/2307-0404.2023.2.283152","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283152","url":null,"abstract":" Sokolova K.V., Podpletnia O.A., Konovalova S.O., Avdieienko A.P., Komarovska-Porokhniavets O.Z., Lubenets V.I., Kovalenko S.I. Continuing our research on compounds that affect urination, we have become interested in N-arylsulfonyl-2-aroylamino-1,4-quinone imines, which combine a quinone matrix with tolylsulfonamide and benzamide fragments with versatile biological activity in their structure, which has a promising value in preventing development of pathological processes in kidneys. Therefore, the search for low-toxic compounds with polyvector activity as a promising approach to the design of drug-like molecules has become an urgent aspect in this regard. The aim of this work was to investigate N-arylsulfonyl-2-aroylamino-1,4-quinone imines and their hydrogenated analogues as promising diuretic agents with antiradical and antibacterial activity using in silico, in vitro and in vivo methodologies. The virtual laboratory of the ProTox-II site is used to predict the toxicity of molecules. The study of compounds affecting the excretory function of the rat kidneys was carried out on 120 white Wistar rats according to the method of E.B. Berkhin under conditions of water stress and spontaneous urination. The interaction of the synthesised compounds with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to study their antiradical activity in vitro. The antibacterial activity of the compounds was studied on test cultures of the bacteria Escherichia coli, Staphylococcus aureus, Mycobacterium luteum and the fungi Candida tenuis, Aspergillus niger by the method of serial dilutions in a liquid nutrient medium. Based on the results of the calculation, it was predicted that N-arylsulfonyl-2-aroylamino-1,4-quinone imines (2) and their hydrogenated analogues (3) have hepato-(immuno-, cyto-) toxicity, carcinogenicity (mutagenicity) similar to natural quinones and diuretics (toxicity class IV). This class of compounds has been shown to have both stimulatory and inhibitory effects on diuresis under condi­tions of water stress and spontaneous urination. At the same time, N-(5-methyl-6-oxo-3-(tosylimino)cyclohexa-1,4-dien-1-yl)benzamide (2.3) was revealed to increase daily diuresis by 67.1% compared with the control, exceeding the effect of «Furosemide» (22.2%). It was found that quinone imines (2.1-2.5) inhibited the formation of the DPPH radical by 25.99-40.09%, while their hydrogenated analogues (3.1 and 3.2) – by 61.56% and 68.28%, respectively, and are more effective acceptors of radicals. The microbiological screening revealed a number of promising compounds that inhibited the growth of S. aureus (compound 2.5, MIC 62.5 μg/ml, MBC 125.0 μg/ml), M. luteum (3.1 and 3.2, MIC 31.2 μg/ml, MBC 62.5 μg/ml) and A. niger (2.1, 2.4 and 3.2, MIC 31.2 μg/ml, MPC 62.5 μg/ml). According to the results of biological studies, among N-arylsulfonyl-2-aroylamino-1,4-quinone imines and their hydrogenated analogues, compound 2.3 has been iden­tified, which competes with «Furosemide» in","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"66 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86273688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of the use of purified bacterial lysates of the most therapy-resistant strains of microorganisms – the causative agents of pustular skin infections in the treatment of acne vulgaris (acne vulgaris)","authors":"S. Zakharov, V.K. Zakharov, V. Gorbuntsov","doi":"10.26641/2307-0404.2023.2.283344","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283344","url":null,"abstract":"The objective of the work was to study the effectiveness of the use of purified bacterial lysates of the most therapy-resistant strains of microorganisms, the causative agents of pustular skin infections, by comparing clinical data and the skin microbiome in patients with acne. The results of the study are based on the data of a comprehensive examination of 35 patients with acne of 2-3 degrees before and after treatment, as well as data of the examination of 15 patients of the control group. Examination methods: clinical examination of the skin, determination of the quantitative composition and microbiological identification of the skin microbiome (bacteria of the genera Staphylococcus, Streptococcus, Propionibacterium Corynebacterium, Micrococcus, Sarcina, Aerococcus, Acinetobacter, Brevibacterium, Citrobacter, Serratia, Lactobacillus; yeast-like fungi of the genera Malassesia and Candida, mold fungi ). In the studied patients with acne, the representative microorganisms of the skin microbiome were Propionibacterium acnes, S. epidermidis, and C. lipophilicum. In patients with acne, the stability of the skin microbiome was carried out due to the redistribution between S. epidermidis and C. lipophilicum. In patients receiving \"Acnevac\"® (Bioveta, a. s.), a component of a complex treatment, along with earlier periods of clinical improvement, there was noted an earlier normalization of the skin microbiome in comparison with patients receiving standard therapy. Approaches to the treatment of acne with antibiotics and chemotherapy require a revision. The state of the skin microbiome broadens knowledge on the pathogenesis of acne and the search for other means for the individual treatment of patients. Positive changes in the skin microbiome under the influence of treatment with purified bacterial lysates of the most therapy-resistant strains of microorganisms, the causative agents of pustular skin infections, make it possible to recommend its use for a longer term – up to 6 months.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"10 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81185326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prosthetic stomatitis in patients with diabetes mellitus: correction of psycho-emotional stress","authors":"V. Dvornyk, I. Lytovchenko, I. Popovych, I. A. Holovanova, I. Dvornyk, O. Muravlova, L.B. Yerus, O. Teslenko","doi":"10.26641/2307-0404.2023.2.283362","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283362","url":null,"abstract":"A high level of chronic emotional stress affects the occurrence and increases the severity of diseases of the oral mucosa and internal pathology. The aim of the work is to increase the effectiveness of the treatment of prosthetic stomatitis in patients with diabetes mellitus against the background of psycho-emotional stress by correcting it. To objectify the state of psycho-emotional stress, in 2005-2015 and in 2022 psychological testing was carried out by the D. Taylor method on 230 people aged 19-70 years, among whom 85 patients were diagnosed with diabetes mellitus – the main group; 145 people were without internal diseases – two control groups. General examination and treatment of patients of the main group – 46 women and 39 men aged 19-70 years was carried out by endocrinologists according to the relevant protocols. Dental examination and treatment of prosthetic stomatitis was carried out in 12 patients with diabetes mellitus who had a high level of general anxiety according to the D. Taylor method. Own method of treatment: after the correction of the prosthesis, sanitation of the oral cavity, applications of thiotriazoline 2% ointment, a course of 7-10 days on the areas of the prosthetic bed, most often subject to trauma were prescribed. For general therapy, an additional stress-protective drug which corrects psycho-emotional stress in patients with diabetes mellitus was prescribed, thiotriazoline, orally, for 30 days. In patients of the main group, a high level of general personal anxiety was recorded in 21.63% of respondents and an average level with a tendency to high – in 54.29%. In many cases, the dentist should know the level of the patient's psycho-emotional stress in order to justify an individual approach to therapeutic measures and maintenance therapy in the choice of drugs and methods. The proposed method of treatment of prosthetic stomatitis has an individual approach, includes the stress-protective drug thiotriazoline, which corrects psycho-emotional stress, allowing to achieve a stable remission of the disease.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"35 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83407473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatopleural fistula as a complication of necrotizing pancreatitis (clinical case)","authors":"V. Desyateryk, O. Kotov, S. Mikhno, Ye.O. Okhotnik, M.P. Krasnov","doi":"10.26641/2307-0404.2023.2.283421","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283421","url":null,"abstract":"Acute pancreatitis is a disease that is accompanied by systemic inflammatory response syndrome and polysystemic dysfunction. Often acute pancreatitis leads to damage to various organs, including: pleura, mediastinum, cardiovascular structures. These complications cause difficulties in diagnosing and treating patients. In this review, we discuss the complications of acute pancreatitis, focusing on the diagnosis and treatment of pancreatopleural fistulas. Pancreatopleural fistula is a rather rare complication observed in patients with both acute and chronic pancreatitis due to traumatic or iatrogenic damage to the pancreatic duct. Conservative treatment with ocreatid combined with stenting of the pancreatic duct currently makes it possible to achieve closure of the fistula in 31-45% of cases, surgical intervention – in 80-90% of cases. The clinical case presented in this article demonstrates the successful treatment of this difficult complication of acute pancreatitis. The authors emphasized the need for timely diagnosis of this condition, as delaying adequate treatment can worsen the prognosis of recovery of patients with pancreatopleural fistulae.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"12 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85925227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Awareness, acceptances and activities as components of the environmental consciousness of youth","authors":"М.Yu. Аntomonov, S.L. Pashynska, M. Sokolova, T. Skochko, О. Rudnytska","doi":"10.26641/2307-0404.2023.2.283407","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283407","url":null,"abstract":"The formation of human health takes place under conditions of both positive and negative influence of the external environment. There is an undoubted connection between human health and the degree of its intervention in the natural environment, which, in turn, is determined by the level of environmental awareness of society. The purpose of this study was to determine the relationship between the levels of awareness, instructions and activities at different levels of environmental consciousness of modern youth. Used methods: bibliographic; questionnaire method (to collect data on the environmental awareness of young people); statistical (for selection and analysis of obtained data); mathematical (for constructing formulas and calculating levels of ecological consciousness and its components). A questionnaire was developed to survey students and high school students in different cities of Ukraine. 285 respondents aged 15 to 24 took part in the survey. In order to compare the level of environmental awareness of young people with  people of other age groups, 53 respondents over the age of 24 were involved in the survey, which was 15,7% of the total number of respondents. The questionnaire was conventionally divided into semantic modules for the possibility of determining the levels of environmental knowledge, instructions and behavior. To achieve the goal of the work, the level of environmental awareness and its components, taking into account gender and age, was assessed. Quantitative values of the levels of the components of environmental consciousness were calculated according to the integral evaluation formulas. For the convenience of assessing the level of the components of environmental consciousness, the quantitative values were ranked in three classes: \"weak\", \"medium\" and \"high\" severity. It was found that young people of both sexes almost do not differ in the high level of guidance. However, the share of girls with a high level of awareness is slightly higher than the share of boys and is significantly higher by a high level of activity. Awareness of the social and individual value of nature increases significantly with age, but readiness for active nature conservation activities does not change with age. The dependence of eco-awareness on the place of residence has been established: in small towns, the eco-awareness of residents is higher than in those who live in large cities. Also, eco-awareness depends on the field of study: students of humanitarian specialties have a significantly higher level of eco-awareness than students of technical universities. The analysis of the obtained results will contribute to the development of ways of forming the ecological consciousness of pupils and students, which is one of the main tasks of ecological and hygienic education and education of young people.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"44 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86658882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the safety of priority carcinogenic substances at the level of hygienic regulations in atmospheric air","authors":"I. Chernychenko, O. Lytvychenko, V. Babii, O.Ie. Kondratenko, O. Rudnytska, D. Hlavachek","doi":"10.26641/2307-0404.2023.2.283402","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283402","url":null,"abstract":"The aim of this work was to assess the risk of carcinogenic substances with a proven danger to humans at the level of current maximum permissible concentrations (MPC). The results were obtained on the basis of the collection and analysis of data on chemical carcinogenic compounds that are included in the national regulatory base of permissible content in the atmospheric air of populated areas, as well as the base of Directives of EU countries and the International Agency for the Study of Cancer using bibliosemantic, bibliographic and hygienic research methods. At the same time, the chemical substances classified by experts from International Agency for Research on Cancer in terms of carcinogenicity to humans were taken into consideration: definitely carcinogenic (group 1), probably (group 2A) and possibly carcinogenic to humans (group 2B). As a result, 52 compounds were identified in the list of carcinogenic substances normalized in atmospheric air, of which 12 substances are assigned to group 1, 13 to group 2A, and 27 to group 2B. In this work, only substances of the first group were subject to in-depth analysis. To assess the danger of substances’ concentrations at the level of maximum permissible concentrations, risk indicators according to the recommended methodical documents were determined. Calculations of the carcinogenic risk caused by the concentrations of these substances at the level of hygienic regulations for the atmospheric air of populated areas proved that only for two compounds (benzo/a/pyrene and formaldehyde) the risk is acceptable and meets the international standards recommended by EU and WHO. For other substances of this group, the carcinogenic risk of exposure is excessive. A conclusion was made regarding the need to review the existing hygienic standards from the standpoint of risk and consider them in the future as annual averages. In order to solve this issue, the criterion scale \"concentration - carcinogenic risk\" was highlighted in the work, with the help of which it was proposed to determine the maximum permissible concentrations according to the indicators of risk acceptable for the conditions of Ukraine.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"11 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87137241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personalized genotype markers of the atopic disorders phenotypes in children","authors":"V. Dytiatkovskyi","doi":"10.26641/2307-0404.2023.2.283346","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283346","url":null,"abstract":"The goal of the study was to elucidate the impact of the single nucleotide variants rs11466749 of the thymic stromal lymphopoietin gene, rs_7216389 of the orsomucoid-1-like protein 3 gene, and rs10052957 of the human nuclear glucocorticoid receptor subfamily 3, group C, member 1 gene on the development of the mono-organic phenotype “atopic eczema” or the poly-organic “atopic eczema + allergic rhinitis/allergic rhino-conjunctivitis”. We recruited 101 patients into the main and 105 into control groups aged from 3 to 18 years old. Patients of the main group suffered from atopic eczema (58 children) and atopic eczema + allergic rhinitis/allergic rhino-conjunctivitis (43 children). Patients of the control group  suffered from the digestive tract pathology. Main group patients were genotyped for the A/A, A/G, G/G of rs11466749, C/T, C/C and T/T of rs_7216389 and A/A, A/G and G/G of rs10052957; patients of the control group were genotyped for the A/A, A/G, G/G of rs11466749, C/T, C/C and T/T of rs_7216389 by polymerase chain reaction in real time with restricted fragment length polymorphism. Results: no significant differences in rs11466749 among the main and control groups, the most common variant is A/A – 55.2% (mono-organic) and 55.8% (poly-organic); T/T rs_7216389 is significantly the most common in poly-organic phenotype – 39.5%; rs10052957: A/G variant is significantly most common in mono-organic phenotype – 51.7% and G/G – in the poly-organic phenotype – 62.8%. The G/G rs11466749 variant has a trending to significance direct 0.173 association and increased odds ratio = 5.85 (0.63-54.31) for the poly-organic phenotype and protective impact onto the mono-organic phenotype -0.173 (0.17 (0.02-1.59); T/T rs7216389 variant increases the risk of poly-organic phenotype: 0.227, odds ratio = 2,79 (1.14-6.85) and decreases the risk of mono-organic” phenotype: -0.227, 0.36 (0.15-0.88); A/G rs_10052957 variant significantly increases the risk the mono-organic phenotype: 0.215, odds ratio = 2.5 (1.08-5.56)) and decreases risk of poly-organic phenotype: 0.215, odds ratio = 0.4 (0.18-0.93); G/G rs_10052957 variant significantly increases the risk of the poly-organic phenotype: 0.263, odds ratio = 2.97 (1.31-6.74)) and decreases for the mono-organic phenotype: -0.263, odds ratio = 0.34 (0.15-0.76)). Genotype variant T/T rs_7216389 of the orsomucoid-1-like protein 3 gene significantly increases the risk of developing the poly-organic atopic phenotype by 2.79 times and protects against the mono-organic atopic phenotype by 0.34 times. G/G genotype variant of rs10052957 of the human glucocorticoid receptor subfamily, group C, member 1 gene significantly increases the risk of developing the poly-organic phenotype by 2.97 times, protecting against mono-organic atopic phenotype by 0.34 times.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"24 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87230084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3-year experience of hyperthermic intravesical chemotherapy use in patients with high risk non-muscular-invasive bladder cancer","authors":"R. Chystiakov, F. Kostyev, O. V. Bondar, V. Lysenko, V. Varbanets","doi":"10.26641/2307-0404.2023.2.283254","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283254","url":null,"abstract":"Cur   rently, the search for additional organ-sparing methods of intravesical therapy for non-muscular-invasive bladder cancer (NMIBC) is actively continuing, which could become an effective alternative to standard treatment using the Bacillus Calmette-Guérin (BCG) vaccine. The aim of this work was to analyze the safety profile and long-term results of treatment of patients with the high-risk non-muscular-invasive bladder cancer who received adjuvant intravesical chemotherapy using the Combat BRS HIVEC® device for local hyperthermia (HIVEC® therapy group; n=53) in comparison with patients who received adjuvant therapy after transurethral resection of bladder performed with the Bacillus Calmette-Guérin vaccine (BCG therapy group; n=54). As a result, the median follow-up was 30 months (range 7-36). According to Common Terminology Criteria for Adverse Events (CTCAE) v 5.0, the most relevant side effects in HIVEC®/BCG therapy groups were adverse events grade 1-2: fever – 1%/8%; dysuria – 9%/13%; bladder spasms – 7%/12%; hematuria – 3%/4% and urinary tract infection – 3%/10%. Tumor recurrence was reported in 23 patients receiving intravesical BCG therapy and in 10 patients receiving intravesical hyperthermic chemotherapy (42.6% versus 18.9%, p=0.008). Tumor progression was recorded in 11 patients receiving intravesical BCG therapy and in 4 patients receiving intravesical hyperthermic chemotherapy (20.4% versus 7.5%, p=0.046). The study allows us to conclude that the method of hyperthermic intravesical chemotherapy has a better safety profile compared to intravesical Bacillus Calmette–Guérin vaccine therapy, while such indicators of oncological efficacy as 3-year recurrence-free survival and the incidence of progression were better in the HIVEC® therapy group.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"90 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76327365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace elements changes in the forebrain and their influence on the rats behavior in elevated plus maze in acute period of mild blast-induced traumatic brain injury","authors":"Y. Kozlova","doi":"10.26641/2307-0404.2023.2.283151","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.283151","url":null,"abstract":"The relevance of the study is related to the high frequency of blast-induced brain injury in both military and civilian populations, which is caused by the use of various types of explosives in military conflicts, including in Ukraine today.  Changes of biometals in the acute period of a mild blast-induced traumatic brain injury, including forebrain that participates in the implementation of various behavioral and cognitive processes, remain unexplained. The study was carried out on 54 sexually mature male Wistar rats, which were divided into 3 groups: experimental (influenced by a baroacoustic wave with an excess pressure of 26-36 kPa, previously anesthetized with halothane and softly fixed), sham (under the influence of halothane and fixation), intact. Behavior was studied in an elevated plus maze. Biometals in the forebrain were determined using energy dispersive X-ray fluorescence analysis. Standard deviation, Mann-Whitney U test (р<0.01, р<0.05), Spearman's correlation coef­ficient (r, р<0.01) were statistically calculated. According to the results of the study, behavioral changes in the elevated plus maze were found in the rats of the experimental group, which indicate the absence of fear on day 1 and 3 and the increase of anxiety on day 7 of the post-traumatic period. Spectral analysis of the forebrain showed significant (р<0.01) changes in the Cu/Fe, Cu/Zn and Zn/Fe ratios, which are the result of iron accumulation due to disruption of the blood-brain barrier. The obtained changes in the ratio of biometals lead to the development of anxiety, which is confirmed by correlation analysis. This indicates that the imbalance of biometals is an important secondary factor in the pathogenesis of blast-induced brain traumatic injury, which is important to consider for diagnosis, treatment and prevention of complications.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"14 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88941686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of cognitive function against the background of partial food derivation and its correction","authors":"A. Kushta","doi":"10.26641/2307-0404.2023.2.282990","DOIUrl":"https://doi.org/10.26641/2307-0404.2023.2.282990","url":null,"abstract":"Tumors of the head and neck account for a significant percentage of all oncopathology. Oral cancer is most often diagnosed. The location of the tumor and its invasion causes swallowing disorders in the preoperative period, which is exacerbated in the postoperative period due to bulky defects. Impaired swallowing leads to reduced nutritional status. Of interest is cognitive function against the background of nutritional deficiency. Metabolic disorders such as obesity are known to adversely affect cognitive function and may even lead to dementia. But it is still unknown how food deprivation affects the cognitive function of rats, as well as the ability of animals to cope with stressful situations, because hunger is one of the models of stressors. Objective – to assess the state of cognitive function and stress resistance against the background of partial food deprivation and its correction in the experiment. The work was performed on 60 adult male rats weighing 280±20 g (M±m) of the Wistar line. Prior to the experimental study, the animals were on partial food deprivation (PFD). After weight loss, rats were divided into 4 groups depending on the dietary adjustment schemes: 1 group - control animals, nutrition according to physiological needs; Group 2, partial food derivation (PFD), without compensatory nutrition; Group 3 – compensatory enteral nutrition with food mixture \"Peptamen\" (PFD+EX); Group 4 – enteral nutrition and the drug Glutargin (PFD+EX+Glutargin). Behavioral response and cognitive function were determined in the open field test and in the CRPA model (conditioned reflex of passive avoidance). Our results show that rats with partial food deprivation are more prone to stress and can not find a sure way out of this situation. Also, throughout the study period, they suffered from cognitive function, as evidenced by the CRPA test data. In the study of resistance to stress on the background of partial food derivation, rats that continued to receive a limited diet by indicators (ambulation, grooming, rearing. defecation) were not stress-resistant, compared with the control group on the background of compensatory nutrition (p<0.05). During all periods of the study of cognitive function there was a change in the rate of stay in the light chamber with a significant (p<0.05) improvement in group 1 – 20.5%, group 3 – 18.36% and group 4 – 19.53%.","PeriodicalId":41059,"journal":{"name":"Medical Perspectives-Medicni Perspektivi","volume":"40 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89107731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}