Antibody ReportsPub Date : 2024-06-10DOI: 10.24450/journals/abrep.2024.e1593
Merina Dhungana, Anna Marchetti
{"title":"The AY165 antibody recognizes the V5-tag by immunofluorescence","authors":"Merina Dhungana, Anna Marchetti","doi":"10.24450/journals/abrep.2024.e1593","DOIUrl":"https://doi.org/10.24450/journals/abrep.2024.e1593","url":null,"abstract":"Recombinant anti-V5 tag antibodies AE408 and AY165 were tested for their ability to recognize an overexpressed V5-tagged protein by immunofluorescence. Only AY165 labeled efficiently cells overexpressing a V5-tagged protein.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":" 90","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2024-01-16DOI: 10.24450/journals/abrep.2023.e1433
Céline Reverdy, J. Rain
{"title":"AZ921 recognizes the endogenous USP7 protein by immunofluorescence in HCT116 cells","authors":"Céline Reverdy, J. Rain","doi":"10.24450/journals/abrep.2023.e1433","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1433","url":null,"abstract":"The antibody AZ921 derived from a synthetic VHH recognizes the endogenous USP7 protein in HCT116 cells by immunofluorescence.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2023-09-01DOI: 10.24450/journals/abrep.2023.e1251
P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca
{"title":"RB792, RB793, RB794, RB795, RB796 and RB797 antibodies recognize a human NDC80 phosphorylated peptide by ELISA","authors":"P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca","doi":"10.24450/journals/abrep.2023.e1251","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1251","url":null,"abstract":"The recombinant antibodies RB792, RB793, RB794, RB795, RB796 and RB797 recognize by ELISA a synthetic phosphorylated peptide from the human NDC80 protein.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126114422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2023-09-01DOI: 10.24450/journals/abrep.2023.e1252
P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca
{"title":"RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806 and RB807 antibodies recognize a human Aurora kinase B (AURKB) phosphorylated peptide by ELISA","authors":"P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca","doi":"10.24450/journals/abrep.2023.e1252","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1252","url":null,"abstract":"The recombinant antibodies RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806, and RB807 were tested by ELISA for their binding to a synthetic phosphorylated peptide from the human Aurora kinase B (AurKB) protein. Antibodies RB805 and RB806 detected both the phosphorylated and non-phosphorylated peptides, whereas the remaining antibodies selectively recognized the phosphorylated peptide.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127575497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2023-07-18DOI: 10.24450/journals/abrep.2023.e1227
Lucie Mencier, Lana Moudarres, Gaelle Najand, Laura Roch, Audrey Ruetsche, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Soyan Dawit, Meg-Mai-Ly Diep, Odyssee Ferrillo, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, S. Durual, C. Guilhen, Tania N. Jauslin
{"title":"AF641, AI954 and AW954 antibodies label markers of the human endoplasmic reticulum by western blot","authors":"Lucie Mencier, Lana Moudarres, Gaelle Najand, Laura Roch, Audrey Ruetsche, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Soyan Dawit, Meg-Mai-Ly Diep, Odyssee Ferrillo, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, S. Durual, C. Guilhen, Tania N. Jauslin","doi":"10.24450/journals/abrep.2023.e1227","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1227","url":null,"abstract":"The recombinant antibodies AF641, AI954 and AW954 detect different markers of the endoplasmic reticulum by western blot.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129740463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2023-06-20DOI: 10.24450/journals/abrep.2023.e1216
B. Bollen Pinto, P. Hammel, Marianne Paolini Bertrand, B. Walder, N. Vuilleumier, O. Hartley
{"title":"Recombinant nanobodies detecting the human cardiac troponin proteins by ELISA","authors":"B. Bollen Pinto, P. Hammel, Marianne Paolini Bertrand, B. Walder, N. Vuilleumier, O. Hartley","doi":"10.24450/journals/abrep.2023.e1216","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1216","url":null,"abstract":"We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms of the human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), the full-length cTnT protein (FL-cTnT) and the cardiac troponin I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes the N-terminal region of cTnT as well as the full-length protein alone and the ITC-complex. RB767, RB770, RB771, RB772 and RB773 recognize the full-length cTnT protein alone or in the ITC-complex but not the N-terminal peptide. RB766, RB768 and RB769 recognize the ITC-complex but not cTnT or cardiac troponin I (cTnI) proteins.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133136011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibody ReportsPub Date : 2023-03-29DOI: 10.24450/journals/abrep.2023.e1143
Soyan Dawit, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Jean-Pierre Aubry-Lachainaye, Cécile Gameiro, S. Durual, Tania N. Jauslin, Audrey Ruetsche, Laura Roch, Meg-Mai-Ly Diep, Odyssee Fer, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, Lucie Mencier, Lana Moudarres, Gaelle Najand, C. Guilhen
{"title":"AD822, AW199, AW200 and AW201 antibodies against the human Fas receptor (CD95) bind the surface of HEK293 cells as revealed by flow cytometry","authors":"Soyan Dawit, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Jean-Pierre Aubry-Lachainaye, Cécile Gameiro, S. Durual, Tania N. Jauslin, Audrey Ruetsche, Laura Roch, Meg-Mai-Ly Diep, Odyssee Fer, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, Lucie Mencier, Lana Moudarres, Gaelle Najand, C. Guilhen","doi":"10.24450/journals/abrep.2023.e1143","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1143","url":null,"abstract":"The recombinant antibodies AD822, AW199, AW200 and AW201 bind HEK293 cells expressing the human Fas receptor protein as assessed by flow cytometry.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128684660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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