ELISA法检测人心肌肌钙蛋白的重组纳米体

Antibody Reports Pub Date : 2023-06-20 DOI:10.24450/journals/abrep.2023.e1216

B. Bollen Pinto, P. Hammel, Marianne Paolini Bertrand, B. Walder, N. Vuilleumier, O. Hartley

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引用次数: 0

摘要

我们检测了抗体RB733、RB734、RB766、RB767、RB768、RB769、RB770、RB771、RB772和RB773对三种不同分子形式的人心肌肌钙蛋白T异构体6 (cTnT)的识别能力:n端肽(nt -cTnT)、cTnT全长蛋白(FL-cTnT)和心肌肌钙蛋白I-T-C复合物(tc -复合物)。我们的研究结果表明，RB734特异性识别cTnT的n端区域，以及全长蛋白单独和itc复合物。RB767、RB770、RB771、RB772和RB773仅识别全长cTnT蛋白或在tc -复合物中，但不识别n端肽。RB766、RB768和RB769识别itc复合物，但不识别cTnT或心肌肌钙蛋白I (cTnI)蛋白。

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Recombinant nanobodies detecting the human cardiac troponin proteins by ELISA

We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms of the human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), the full-length cTnT protein (FL-cTnT) and the cardiac troponin I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes the N-terminal region of cTnT as well as the full-length protein alone and the ITC-complex. RB767, RB770, RB771, RB772 and RB773 recognize the full-length cTnT protein alone or in the ITC-complex but not the N-terminal peptide. RB766, RB768 and RB769 recognize the ITC-complex but not cTnT or cardiac troponin I (cTnI) proteins.

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Antibody Reports

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