Current Protocols in Neuroscience最新文献_第9页

Hybridization Histochemistry of Neural Transcripts 神经转录本的杂交组织化学

Current Protocols in Neuroscience Pub Date : 2018-01-22 DOI: 10.1002/cpns.39

W. Scott Young, June Song, Éva Mezey

引用次数: 2

A Guide to Robust Statistical Methods in Neuroscience 神经科学稳健统计方法指南

Current Protocols in Neuroscience Pub Date : 2018-01-22 DOI: 10.1002/cpns.41

Rand R. Wilcox, Guillaume A. Rousselet

引用次数: 85

Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit 使用巧合报告生物电路的定量高通量筛选

Current Protocols in Neuroscience Pub Date : 2017-04-10 DOI: 10.1002/cpns.27

Brittany W. Schuck, Ryan MacArthur, James Inglese

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引用次数: 3

Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability 异硫氰酸荧光素(FITC)-葡聚糖外渗测定血脑屏障通透性

Current Protocols in Neuroscience Pub Date : 2017-04-10 DOI: 10.1002/cpns.25

Reka Natarajan, Nicole Northrop, Bryan Yamamoto

引用次数: 55

CRISPR/Cas9-Mediated Gene Knockout in the Mouse Brain Using In Utero Electroporation 利用子宫电穿孔技术，CRISPR/ cas9介导的小鼠大脑基因敲除

Current Protocols in Neuroscience Pub Date : 2017-04-10 DOI: 10.1002/cpns.26

Yohei Shinmyo, Hiroshi Kawasaki

引用次数: 10

Systems Genetic Analysis in GeneNetwork.org GeneNetwork.org系统遗传分析

Current Protocols in Neuroscience Pub Date : 2017-04-10 DOI: 10.1002/cpns.23

Clarissa C. Parker, Price E. Dickson, Vivek M. Philip, Mary Thomas, Elissa J. Chesler

{"title":"Systems Genetic Analysis in GeneNetwork.org","authors":"Clarissa C. Parker, Price E. Dickson, Vivek M. Philip, Mary Thomas, Elissa J. Chesler","doi":"10.1002/cpns.23","DOIUrl":"10.1002/cpns.23","url":null,"abstract":"<p>Genome-wide association studies (GWAS) have emerged as a powerful tool to identify alleles and molecular pathways that influence susceptibility to psychiatric disorders and other diseases. Forward genetics using mouse mapping populations allows for a complementary approach that provides rigorous genetic and environmental control. In this unit, we describe techniques and tools that reduce the technical burden traditionally associated with genetic mapping in mice and enhance their translational utility to human psychiatric disorders. We provide guidance on choosing the appropriate mapping population, discuss the importance of phenotype, and offer detailed instructions on using the Web-based resource GeneNetwork to aid neuroscientists in better understanding the mechanisms through which genes influence behavior. We believe that the continued development of mouse mapping populations, genetic tools, bioinformatics resources, and statistical methodologies should remain a parallel strategy by which to investigate the genetic and environmental underpinnings of psychiatric disorders and other diseases in humans. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34904378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Intracerebral Injections and Ultrastructural Analysis of High-Pressure Frozen Brain Tissue 高压冷冻脑组织的脑内注射及超微结构分析

Current Protocols in Neuroscience Pub Date : 2017-01-03 DOI: 10.1002/cpns.22

Marie-Theres Weil, Torben Ruhwedel, Wiebke Möbius, Mikael Simons

引用次数: 6

Electroporation in the Rodent Embryonic Brain Using Whole Embryo Culture System 利用全胚胎培养系统对啮齿动物胚胎脑进行电穿孔

Current Protocols in Neuroscience Pub Date : 2017-01-03 DOI: 10.1002/cpns.21

Takako Kikkawa, Masanori Takahashi, Noriko Osumi

引用次数: 1

The Five-Choice Continuous Performance Task (5C-CPT): A Cross-Species Relevant Paradigm for Assessment of Vigilance and Response Inhibition in Rodents 五选择连续性能任务（5C-CPT）：评估啮齿动物警惕性和反应抑制的跨物种相关范式

Current Protocols in Neuroscience Pub Date : 2017-01-01 DOI: 10.1002/cpns.20

Zackary A. Cope, Jared W. Young

{"title":"The Five-Choice Continuous Performance Task (5C-CPT): A Cross-Species Relevant Paradigm for Assessment of Vigilance and Response Inhibition in Rodents","authors":"Zackary A. Cope, Jared W. Young","doi":"10.1002/cpns.20","DOIUrl":"https://doi.org/10.1002/cpns.20","url":null,"abstract":"Deficits in the domains of attention and response inhibition are central to many psychiatric disorders. As such, animal models of disorders purporting to replicate these behavioral deficits first require tests that can accurately assess the behaviors with high fidelity. The gold-standard clinical test of attention and response inhibition is the continuous performance test (CPT). Although there are a number of CPTs, all share the premise of responding to target stimuli and inhibiting from responding to non-target stimuli. The recently developed rodent five-choice CPT (5C-CPT) requires similar behavioral responses, enabling signal detection parameter calculations. With demonstrable feasibility for rodent testing, the 5C-CPT permits/facilitates: (1) delineation of neural mechanisms underlying these behaviors; (2) multifactorial analyses of the complex interplay between genetic and environmental manipulations relevant to psychiatric disorders; and hence (3) development of novel targeted treatments. All data to date indicate that the rodent 5C-CPT described here has direct translatability to clinical CPTs, producing equivalent measures of behavior in experimental animals to those assessed in humans. The 5C-CPT task provides an important tool toward delineating these mechanisms and developing treatments. However, it is also complex, with long training times and nuances requiring a thorough understanding before utilization. This unit will enable researchers to avoid potential missteps, greatly increasing the likelihood of success. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.20","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71928412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Visualizing Changes in Neuronal Dendritic Morphology in Response to Stress and Pharmacological Challenge 可视化神经元树突形态在应激和药理学挑战反应中的变化

Current Protocols in Neuroscience Pub Date : 2017-01-01 DOI: 10.1002/cpns.18

Cara L. Wellman

引用次数: 4