Natural Science: Journal of Science and Technology最新文献

Sensitivity and Specificity of Molecular-Based Pathogen Detection Method in Refillable Drinking Water Samples 基于分子检测方法在可重复灌装饮用水样品中的敏感性和特异性

Natural Science: Journal of Science and Technology Pub Date : 2023-03-25 DOI: 10.22487/25411969.2022.v11.i02.16217

A. Achyar, I. Putri, D. Putri

{"title":"Sensitivity and Specificity of Molecular-Based Pathogen Detection Method in Refillable Drinking Water Samples","authors":"A. Achyar, I. Putri, D. Putri","doi":"10.22487/25411969.2022.v11.i02.16217","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16217","url":null,"abstract":"The existence of refillable drinking water depots helps the community to get affordable and practical drinking water. However, poor quality drinking water will, however, have an effect on health. One of the quality parameters of drinking water that is suitable for consumption is not contaminated by the bacteria Escherichia coli, Salmonella sp. and Escherichia sp. Measurement of the quality of drinking water, in addition to microbiological tests, can be carried out molecularly using PCR (Polymerase Chain Reaction) method. Therefore, the aim of this study was to examine the sensitivity and specificity of PCR for detection of drinking water pathogens. DNA was extracted from cultures of E. coli, Salmonella sp., Escherichia sp. and some non-coliform bacteria. PCR was performed separately using primer pairs of E. coli-AA-Forward and E.coli-AA-Reverse, Salmonella-OY-Forward and Salmonella-OY-Reverse, E. coli-DB-Forward and E. coli-DB-Reverse. The results of the PCR sensitivity showed that the minimum amount of DNA that can be detected by this method were 0.0025 ng/µL, 0,0005 ng/µL, 0,04 ng/µL for E. coli, Salmonella sp., Escherichia sp., respectively. The results of the PCR specificity of each primer pairs indicated that these methods were able to detect each coliform bacterium specifically according to PCR product size of ± 417 bp, ± 559 bp and ± 815 bp for E. coli, Salmonella sp., Escherichia sp., respectively.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132372492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of moringa leaf infusion on Escherichia coli isolate from Musca domestica L 辣木叶浸液对家蝇大肠杆菌的抑菌活性研究

Natural Science: Journal of Science and Technology Pub Date : 2023-03-25 DOI: 10.22487/25411969.2022.v11.i02.16166

{"title":"Antimicrobial activity of moringa leaf infusion on Escherichia coli isolate from Musca domestica L","authors":"","doi":"10.22487/25411969.2022.v11.i02.16166","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16166","url":null,"abstract":"The Moringa oleifera can be used as a medium for the prevention and treatment of infectious diseases such as diarrhea. Diarrhea can be caused by Escherichia coli, which house flies can transmit. Moringa oleifera leaves have antimicrobial substances such as flavonoids, triterpenoids, steroids, saponins and tannins. This aim of the study was to determine the minimum inhibitory concentration of M. oleifera infusion on the growth of E. coli. This research is a laboratory experiment with Well Diffusion Agar method and dilution technique. The materials used in the study were M. oleifera leaf infusion with concentrations of 50%, 40%, 30%, 20%, and 10%, while 0% was a negative control (Aquadest). E. coli isolates were obtained from M. domestica. The results showed that the inhibition zone formed at concentrations of 50%, 40%, 30%, 20%, 10%, and 0% was 15.55 mm, 13.80 mm, 13.10 mm, 10.10 mm, and 0 mm, respectively. The minimum inhibitory concentration of leaf infusion against E. coli isolates was 25%, and the minimum bactericidal concentration could not be determined because all concentrations tested showed bacterial growth. Instead, the lowest inhibitory concentration was 25%. In concentrations of 50%, 40%, and 30%, M. oleifera leaf infusion has potent antibacterial action; at concentrations of 20%, it has moderate antimicrobial activity; and at concentrations of 10%, it is unable to inhibit the growth of E. coli. Further research is needed to determine the value of the minimum bactericidal concentration at concentrations above 25%, and it is necessary to test it on other digestive tract pathogenic bacteria.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129127537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Potential of Catfish (Clarias batrachus) and Snakehead Fish (Chana striata) Mucus on Bacterial Coliform Growth and its Application as the Organic Face Mask and Lipstick 鲶鱼(Clarias batrachus)和黑鱼(Chana striata)黏液对细菌大肠菌群生长的抑菌潜力及其在有机面膜和口红中的应用

Natural Science: Journal of Science and Technology Pub Date : 2023-03-25 DOI: 10.22487/25411969.2022.v11.i02.16171

Wenny Pintalitna Tarigan, C. U. Tarigan, Cherline Halim, J. Valerie, Vionita Itanza, Russel Oeintz

{"title":"Antimicrobial Potential of Catfish (Clarias batrachus) and Snakehead Fish (Chana striata) Mucus on Bacterial Coliform Growth and its Application as the Organic Face Mask and Lipstick","authors":"Wenny Pintalitna Tarigan, C. U. Tarigan, Cherline Halim, J. Valerie, Vionita Itanza, Russel Oeintz","doi":"10.22487/25411969.2022.v11.i02.16171","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16171","url":null,"abstract":"Mucus from snakehead fish (Chana striata) and catfish (Clarias batrachus) has the potential to be employed in organic cosmetics and wound healing. The aim of this study is to create antimicrobials from Clarias batrachus and Chana striata mucus that may inhibit the growth of coliform bacteria. It also seeks to establish the minimum inhibitory concentration of Clarias batrachus and Chana striata mucus extracts against the growth of Escherichia coli when cocoa extract is added as an internal ingredient for making face masks and organic lipstick. The content of anthocyanin in cocoa beans used as a natural colouring agent that can replace synthetic dyes. The pooled fish mucus was extracted with succeeding centrifugation and filtration. The acidic mucus extracts were tested for antimicrobial-inhibitory effects and minimum inhibitory concentration (MIC) by agar-overlay diffusion and plate dilution method, respectively. The results showed that all fish mucus extracts exhibited antimicrobial effects against tested pathogens with catfish exhibiting the highest inhibitory effects against the bacteria sample as compared to the broad-spectrum antibiotic control. Interestingly, their mucus revealed inhibitory effects against bacteria at the lowest concentration (1:4 dilution). The present findings revealed the potential antimicrobial use of freshwater fish mucus against medically-important pathogens","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130043105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of Specific Primer for Methallothionein Gene of Tor Fish (Tor tambra) 托尔鱼甲基硫蛋白基因特异性引物的设计

Natural Science: Journal of Science and Technology Pub Date : 2023-03-08 DOI: 10.22487/25411969.2022.v11.i02.16216

Y. Atifah, Afifatul Achyar

引用次数: 0

The Green chili to reduce formaldehyde in tofu by the spectrophotometric method using Schiff reagent 用希夫试剂分光光度法测定了青椒对豆腐中甲醛的还原作用

Natural Science: Journal of Science and Technology Pub Date : 2023-03-08 DOI: 10.22487/25411969.2022.v11.i02.16144

Edy Agustian Yazid, Roihatul Zahroh, Agita Anung Hadiyati

{"title":"The Green chili to reduce formaldehyde in tofu by the spectrophotometric method using Schiff reagent","authors":"Edy Agustian Yazid, Roihatul Zahroh, Agita Anung Hadiyati","doi":"10.22487/25411969.2022.v11.i02.16144","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16144","url":null,"abstract":"Tofu is a processed soybean food ingredient that is widely consumed by the public. Besides having a high nutritional content, tofu is also relatively cheap and easy to obtain. Tofu has a short shelf life and is easily damaged, so producers often add the preservative formaldehyde, which has been banned for use. Formalin or formaldehyde is not included in the list of food additives because they are toxic and harmful to health. This study was aimed to determine the reduction of formalin levels in white tofu by soaking green chilies. The study was carried out experimentally with the spectrophotometric method using the Schiff reagent. Absorbance measurements were carried out at a maximum wavelength of 580 nm. Parameters observed were the addition of 0% green chili solution concentration 0%; 2.5%; 5.0%; 7.5%; 10%; and 12.5% for 10 minutes of continuous immersion; 20 minutes; and 30 minutes. Based on the results of the study, it was found that each addition of green chili solution concentration reduced the formalin level along with the soaking time. The addition of 12.5% green chili solution for 30 minutes resulted in the greatest decrease in formalin levels, namely 69.82%. Based on these results it was concluded that the addition of green chili solution could reduce formalin in tofu.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"591 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115104940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Physical Evaluation of Sunscreen Cream with Methanol Extract of Euchema cottonii 棉草甲醇提取物防晒霜的配方及物理性能评价

Natural Science: Journal of Science and Technology Pub Date : 2022-12-31 DOI: 10.22487/25411969.2022.v11.i02.16180

Devi Wulansari, Aripudin, Susi Ratnaningtyas, Sri Suminar Gopurullah

引用次数: 1

Physical and chemical conditions of waters for seaweed cultivation in Morowali, Central Sulawesi, Indonesia 印度尼西亚苏拉威西岛中部Morowali海藻养殖水域的物理和化学条件

Natural Science: Journal of Science and Technology Pub Date : 2022-10-17 DOI: 10.22487/25411969.2022.v11.i01.15860

引用次数: 1

Total phenol content of accessions of Kencur (Kaempferia galanga L.) at different altitudes 不同海拔地区山柰种质总酚含量的变化

Natural Science: Journal of Science and Technology Pub Date : 2022-10-17 DOI: 10.22487/25411969.2022.v11.i01.15696

Subaryanti Subaryanti, T. Triadiati, Y. C. Sulistyaningsih, D. I. Pradono

{"title":"Total phenol content of accessions of Kencur (Kaempferia galanga L.) at different altitudes","authors":"Subaryanti Subaryanti, T. Triadiati, Y. C. Sulistyaningsih, D. I. Pradono","doi":"10.22487/25411969.2022.v11.i01.15696","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15696","url":null,"abstract":"Kencur (Kaempferia galanga L.) is one of the potential medicinal plants with a high enough market demand so that the cultivation of kencur is still quite promising. The purpose of this study was to analyze the effect of altitude on the total phenol content of several accessions of kencur. The research was carried out at the Tropical Biopharmaceutical Laboratory, Bogor Agricultural University, from June to November 2017. The treatment tested was kencur accession rhizome taken from two altitudes, 214 m asl (lowlands) and 780 m asl (highlands). 10 g of kencur accession rhizome powder was added with methanol in a ratio of 1:10, then macerated while shaking using a magnetic stirrer (25 °C; 180 min). The extract was filtered and the filtrate was dried using a rotary vacuum evaporator (50 rpm; 60 °C) until a dry crude extract was obtained. Total phenol was tested by the Folin-Ciocalteu method at a wavelength of 730 nm. The results showed that altitude significantly affected the total phenol content of the kencur accession rhizome extract. The highest total phenol content value was obtained from Galesia 1 (GAL 1) in the lowlands of 17.92 mg/g (equivalent to gallic acid/GAE) and in the highlands obtained from PBG accession (Purbalingga) of 24.85 GAE mg/g compared to other accessions.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128580118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiinflammation activity of Muntingia calabura L. leaves ethanol, ethylacetate and chloroform extracts 山楂叶乙醇、乙酸乙酯和氯仿提取物的抗炎活性

Natural Science: Journal of Science and Technology Pub Date : 2022-10-17 DOI: 10.22487/25411969.2022.v11.i01.15699

Nova Rahma Widyaningrum, Sri Saptuti Wahyuningsih, Cipto Priyono

{"title":"Antiinflammation activity of Muntingia calabura L. leaves ethanol, ethylacetate and chloroform extracts","authors":"Nova Rahma Widyaningrum, Sri Saptuti Wahyuningsih, Cipto Priyono","doi":"10.22487/25411969.2022.v11.i01.15699","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15699","url":null,"abstract":"Muntingia calabura L. leaves extract are reported rich in flavonoidal compounds which shows a potential activity such as antidiabetic, antipyretic, analgesic and anti-inflammatory. Several subgroups of flavonoid compounds include flavones, flavanones, flavans and biflavans. This study aimed to analyze the anti-inflammatory activity of ethanolic, ethylacetate, and chloroform extracts of M. calabura L. leaves. The extraction process was carried out by maceration, where the yield of the three solvents were 19.14%; 8.41% and 6.59% w/w, respectively. The anti-inflammatory activity was carried out by observing a decrease in edema due to 1% carrageenan induction after 4 hours. The statistical results of anti-inflammatory activity showed that the ethanol extract had a significant difference with the positive control of p value < 0.05 in which a dose of 240 mg showed a decrease in edema volume (IEV) that was closed to the positive control (acetosal = 52.12%) which was 62.51% compared to the dose 60 mg and 120 mg. Furthermore, the % IEV of ethyl acetate extract of M. calabura L. leaves at a dose of 60, 120, and 240 mg were 36.30%, 26.83%, 24.24%, respectively. These results demonstrated that an average level of anti-inflammatory activity was 22.89%, and was closed to the dose of 500 mg/kg of acetosal-induced mice. Finally, the chloroform extract with the dose of 60 mg, 120 mg, 240 mg had a significant value of p < 0.05 with a positive control (acetosal). It could be concluded that the ethanol, ethylacetate and chloroform extracts of M. calabura L. leaves may have anti-inflammatory potential with acetosal as a comparison.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133693485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effectivity of hand antiseptic against Staphylococcus aureus 手部消毒液对金黄色葡萄球菌的有效性

Natural Science: Journal of Science and Technology Pub Date : 2022-10-17 DOI: 10.22487/25411969.2022.v11.i01.15853

G. M. Nurdin, Nur Hidayah, Naura Nazifa R. Hidayat, Erpi Nurdin

引用次数: 0