D.A. Shergin, A.P Yakovlev, P. Gorelkin, S.V. Salikhov, A. Erofeev
{"title":"Latest Advances in Scanning Ion-Conductance Microscopy and Nanopipette Systems for Single-Cell Analysis under Serial Conditions","authors":"D.A. Shergin, A.P Yakovlev, P. Gorelkin, S.V. Salikhov, A. Erofeev","doi":"10.55959/msu0579-9392.78.2360701","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2360701","url":null,"abstract":"Studying single cells opens opportunities for understanding many biological processes and, as a consequence, plays an important role in the development of biomedicine. Scanning Ion Conductance Microscopy (SICM) and nanopipette systems for the analysis of single cells represent a powerful tool for investigating the morphological, mechanical, and biochemical features of living cells with nanometer spatial resolution under near-natural conditions. Over the last decades, SICM has shown great success in studying and manipulating single cells, and the non-contact nature of the system, combined with the unique features of nanopipettes used as probes, has opened new possibilities for the application of such systems in biomedical applications. This review reflects the latest trends and directions in the development of SICM and nanopipette systems for the single-cell analysis.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"23 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Guggenheim equation for a system of hard spheres and its generalization","authors":"P. N. Nikolaev","doi":"10.55959/msu0579-9392.78.2360101","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2360101","url":null,"abstract":"In the present work, for the first time, the generalized Guggenheim equation for a system of hard spheres is obtained based on the use of the Euler accelerated convergence method. This equation allows taking into account an arbitrary number of known virial coefficients. For a metastable region, this equation is generalized to the case of taking into account the asymptotic behavior of the free energy at high densities. The resulting expression for the compressibility of the homogeneous phase of a system of hard spheres describes the data of a numerical experiment within their accuracy.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"27 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Boos, V. Bunichev, P. Volkov, L. Dudko, M. Perfilov
{"title":"Separation of pair and single top quark production in tWb-associated final state using a neural network","authors":"E. Boos, V. Bunichev, P. Volkov, L. Dudko, M. Perfilov","doi":"10.55959/msu0579-9392.78.2360201","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2360201","url":null,"abstract":"The paper presents a method for separating the contribution of pair and single top quark production in\u0000tWb-associated final state using a neural network. The proposed method makes possible to calculate such\u0000processes in a gauge-invariant way with fully taking into account interference contributions and dividing the\u0000phase space into single-resonant and double-resonant regions, which is necessary to increase the accuracy of\u0000the search for possible deviations from the predictions of the Standard Model in these processes. To train the\u0000neural network, the optimized set of observables is used to separate single-resonance and double-resonance\u0000contributions to the overall process. The use of this method allows us to avoid the disadvantages inherent\u0000in the schemes used in collider physics for calculating the processes of tWb-associated top quark production\u0000with the removal of Feynman diagrams, which leads to violation of gauge invariance, or the addition of a\u0000subtraction scheme, which leads to the appearance of negative weights for the part of simulated events. The\u0000proposed method can be used to increase the efficiency of searching for deviations from the predictions of the\u0000Standard Model in the interaction of the top quark with the W boson and b quark.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":" 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138959902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Kovaliova, T.Yu. Kiseleva, T. F. Grigoreva, V. Zhornik, E. Devyatkina, S. Vosmerikov
{"title":"Mechanical alloying in compositions iron–titanium–carbon (diamond)","authors":"S. Kovaliova, T.Yu. Kiseleva, T. F. Grigoreva, V. Zhornik, E. Devyatkina, S. Vosmerikov","doi":"10.55959/msu0579-9392.78.2360501","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2360501","url":null,"abstract":"X-ray diffraction analysis, scanning electron microscopy and M¨ossbauer spectroscopy have been applied to the study of Fe-xTi (x=20–40 wt.%), Fe/xTi-diamond and Fe–Ti–C powders structure formation under the conditions of an AGO-2 planetary ball mill. It has been shown that metals with high melting points and limited solubility under high-energy mechanical action for 20 min interact to form an alloy of nanostructured iron and grain-boundary phases similar to solid solutions of Fe(Ti), Ti(Fe) and FeTi with a total content of 22–24 %. In the Fe-20wt.%Ti composition, titanium reaches an X-ray amorphous state, and when the titanium content is above 20 wt.% 𝛼-Ti crystalline phase remains in an amount of 5–10 wt.%. When the Fe/xTi-diamond mixture is mechanically activated, composite particles with a metal matrix structure with a diamond size of 0.3–1.5 𝜇m are formed. It has been established titanium accelerates the process of grain-boundary and volumetric interaction of iron with diamond and titanium carbide. In the powder compositions under study, a solid solution is formed based on iron and TiC, Fe3C compounds with their total content: Fe/40 wt.%Ti-diamond up to 62%; Fe/TiC up to 34%. Under similar conditions in a diamond-iron mixture, the grain-boundary phases of the solid solution do not exceed 26%. The formation of graphite has not detected by X-ray diffraction.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"504 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. S. Aleshnovskii, A. Bezrukova, V. K. Avilov, V. A. Gazaryan, Y. Kurbatova, O. Kuricheva, A. Chulichkov, N. E. Shapkina
{"title":"Recovering of gaps in the time series of CO2 concentration and air temperature using methods of mathematical statistics","authors":"V. S. Aleshnovskii, A. Bezrukova, V. K. Avilov, V. A. Gazaryan, Y. Kurbatova, O. Kuricheva, A. Chulichkov, N. E. Shapkina","doi":"10.55959/msu0579-9392.78.2330101","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2330101","url":null,"abstract":"The article is devoted to the problem of recovering gaps in the data series of experimental long-term\u0000continuous high-frequency observations of carbon dioxide concentration and air temperature. The study was\u0000carried out on the example of the results of observations of an automatic ecological and climatic station\u0000located in a tropical monsoon forest on the territory of south Vietnam (Dong Nai Nature Reserve). Omissions\u0000in the series of observations, as a rule, are random and are caused by technical malfunctions of the instrument\u0000base. Correctly recovered series of observations allow us to estimate the temporal variability of the observed\u0000parameters on different time scales. Within the framework of this study, options for recovering the continuity\u0000of time series based on the methods of mathematical statistics - autoregression (ARIMA) and the method of\u0000linear prediction were considered. A comparative analysis of the accuracy of restoring omissions by various\u0000methods is given.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130974355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cross sections of partial photoneutron reactions: the problems with reliability and new data","authors":"Davydov A.I., Varlamov V.V., Orlin V.N.","doi":"10.55959/msu0579-9392.78.2330205","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2330205","url":null,"abstract":"The (𝛾, 1𝑛), (𝛾, 2𝑛) and (𝛾, 3𝑛) reactions cross sections the majority of which were obtained using beams of quasimonoenergetic annihilation photons were discussed. The significant systematic uncertainties of the experimental results far exceeding the achieved statistical accuracy were considered. The results of experiments for about 50 nuclei from 51V to 209Bi were analyzed using objective physical criteria of data reliability. It was shown that in many cases the systematic uncertainties of experimental cross sections were significant and data do not satisfy physical criteria. The experimental theoretical method for evaluation of cross sections satisfying those criteria was described. The systematic uncertainties of several types due to shortcomings of the method of photoneutron multiplicity sorting used in experiments and specific features of neutron detectors used. The new evaluated reliable cross sections of the partial reactions and total photoneutron reaction (𝛾, 1𝑛)+(𝛾, 2𝑛)+(𝛾, 3𝑛) were presented.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"247 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115004117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Tsiniaikin, G. Presnova, I. Bozhev, A. A. Skorik, M. Rubtsova, A. Trifonov, O. Snigirev, V. Krupenin, D. Presnov
{"title":"Nanoscale biosensor with integratedthermoregulation controller\u0000for DNA diagnostics.","authors":"I. Tsiniaikin, G. Presnova, I. Bozhev, A. A. Skorik, M. Rubtsova, A. Trifonov, O. Snigirev, V. Krupenin, D. Presnov","doi":"10.55959/msu0579-9392.78.2320701","DOIUrl":"https://doi.org/10.55959/msu0579-9392.78.2320701","url":null,"abstract":"We present a CMOS compatible technique for fabrication a sensor system based on field-effect transistors\u0000with a nanowire channel with an integrated thermoregulation elements. The proposed system provides the\u0000necessary temperature regimes for many bioanalytical studies. Field-effect transistors with a nanowire channel\u0000were fabricated using of reactive-ion etching of the upper layer of a silicon on insulator through a mask formed\u0000by electron beam lithography. Titanium thermoresistive strips for temperature control were located on the\u0000surface of the chip nearby to the nanowire transistors. Their fabrication is carried out simultaneously with the\u0000formation of contact pads to the transistor electrodes, which made it possible to avoid additional technological\u0000steps. A demonstration of a system with a built-in temperature controller for the determination of nucleic\u0000acids was carried out on model oligonucleotides. Increasing the operating temperature of the device to the\u0000ranges at which DNA hybridization occurs most efficiently allows increasing specificity and avoiding false\u0000positive results, as well as reducing analysis time. The possibility of heating up to 85–90∘C allows you to\u0000reuse such devices.","PeriodicalId":399279,"journal":{"name":"Seriya 3: Fizika, Astronomiya","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126096059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}