应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00643
Z. Tan, Q. Fu, G. Peng, Hongjuan Yuan, Yanbo Cheng, Yuan Jiang
{"title":"Identification and Characterization of Endophytic Diazotrophs Isolated from Cymbopogon caesius and Miscanthus floridulus: Identification and Characterization of Endophytic Diazotrophs Isolated from Cymbopogon caesius and Miscanthus floridulus","authors":"Z. Tan, Q. Fu, G. Peng, Hongjuan Yuan, Yanbo Cheng, Yuan Jiang","doi":"10.3724/SP.J.1145.2013.00643","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00643","url":null,"abstract":"In order to investigate the diversity of endophytic diazotrophs in Cymbopogon caesius and Miscanthu floridulus, thirty and thirty-six isolates were obtained respectively from these two forage grasses by using two selective medium without nitrogen sources in combination with acetylene reduction assay. The sixty-six endophytic diazotrophs were assigned to 10 groups (61 strains, at least 3 for each group from I to X) and five ungrouped strains by analysis of SDS-PAGE (dodecyl sulfate sodium salt polyacrylamide gel electrophoresis) patterns of whole-cell protein electrophoresis and IS-PCR (Insertion sequence-based PCR) DNA fingerprinting. 16S rDNA sequencing analysis of representative strains of each group showed that these strains were closely related to Burkholderia cepacia, Enterobacter cloacae, Phytobacter diazotrophicus, Enterobacter asburiae, Herbaspirillum seropedicae, Cronobacter sakazakii, Enterobacter oryzae, Klebsiella pneumoniae, Enterobacter dissolvens, Azospirillum amazonense, and Cronobacter dublinensis, indicating the high diversity of endophytic diazotrophs colonized in the host plants of C. caesius and M. floridulus. The results of physiological and biochemical tests showed that some isolates had the ability of ammonia secreting, indole producing, and phosphorus and potassium dissolving, suggesting the potential application in agricultural practices. Fig 2, Tab 3, Ref 38","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69811637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00727
Wei Kang, Yue Sun, Lufeng Sun, Zhao-li Xu, Jianglong Que
{"title":"Distribution of Phytoplankton in Radial Sand Ridge Area in North Jiangsu Shoal: Distribution of Phytoplankton in Radial Sand Ridge Area in North Jiangsu Shoal","authors":"Wei Kang, Yue Sun, Lufeng Sun, Zhao-li Xu, Jianglong Que","doi":"10.3724/SP.J.1145.2013.00727","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00727","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00587
Lin Yongming, Wei-Dong Yu, Yi Liu, Shujun Yan, W. Hong, Can Chen, Jian Li, Cheng-zhen Wu
{"title":"Characteristics of Branching Pattern and Crown Morphology of Casuarina equisetifolia L. in Different Coastal Distances: Characteristics of Branching Pattern and Crown Morphology of Casuarina equisetifolia L. in Different Coastal Distances","authors":"Lin Yongming, Wei-Dong Yu, Yi Liu, Shujun Yan, W. Hong, Can Chen, Jian Li, Cheng-zhen Wu","doi":"10.3724/SP.J.1145.2013.00587","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00587","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00704
C. Ke, Bisha Wu, Qin Shao, Wei Yang, Jianzhong Huang
{"title":"Construction of Saccharomyces cescerevisiae Mutant with Overexpression of PDC1 Gene: Construction of Saccharomyces cescerevisiae Mutant with Overexpression of PDC1 Gene","authors":"C. Ke, Bisha Wu, Qin Shao, Wei Yang, Jianzhong Huang","doi":"10.3724/SP.J.1145.2013.00704","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00704","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00630
Y. Qiong, L. Hui, Zuo Qinyue, Qiu Lin, Chen Li, Luo Ke, Tu Shengbin
{"title":"Analysis of Genetic Variation and DNA Methylation Alteration in Autotetraploid and Diploid Rice","authors":"Y. Qiong, L. Hui, Zuo Qinyue, Qiu Lin, Chen Li, Luo Ke, Tu Shengbin","doi":"10.3724/SP.J.1145.2013.00630","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00630","url":null,"abstract":"DNA methylation plays an important role in regulating the process of growth and development in eukaryotes.The purpose is to investigate the genome and methylation differences between diploid and tetraploid,explore the genetic differences and genomic DNA methylation level and pattern changes.The diploid and autotetraploid rices Gang 46B,Minghui63,R725,Chenghui149,Haitian and Shixiang were analyzed by SSR and MSAP technique.The results showed that most genomes of the autotetraploid and diploid rice were the same except some sites with three pair of primers preferably polymorphic and specific bands amplified in the different ploidy rice.The consequences of MSAP suggested that the total methylation ratio in autotetraploid rice(24.0%) was significantly less than the diploid rice(28.15%);The full methylation ratio and hemimethylation ratio were different between the autotetraploid and diploid rices.The averaged full methylation rate and hemimethylation rate were 6.24% and 22.03%,respectively for diploid rice,7.44% and 16.61% for autotetraploid rice.The degree of methylation did not increase due to polyploidy,indicating that diploid and tetraploid DNA methylation of the same species of rice changed.The pattern changes of the DNA methylation in the rice of various ploidy was further analyzed,which showed that demethylation occurred in autotetraploid rice on an average of about 11.6% sites,methylations on about 24.11% sites,and the proportion of genes with methylation significantly higher than that with demethylation.The methylation of some functional genes could induce gene silencing to ease the impact of genome doubling dose effect.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69811240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00694
H. Feng, Yongtao Li, Gan Zhang, Chunling Luo
{"title":"Metal Biosorption by Cochliobolus lunatus with High Cadmium Resistance: Metal Biosorption by Cochliobolus lunatus with High Cadmium Resistance","authors":"H. Feng, Yongtao Li, Gan Zhang, Chunling Luo","doi":"10.3724/SP.J.1145.2013.00694","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00694","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69811736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00718
H. Dai, Huaijiang He, Xiu-hai Zhao, Chunyu Zhang, Jinsong Wang, Song Yang
{"title":"Biomass Allocation Patterns and Allometric Models of Two Dominant Tree Species in Broad-leaved and Korean Pine Mixed Forest: Biomass Allocation Patterns and Allometric Models of Two Dominant Tree Species in Broad-leaved and Korean Pine Mixed Forest","authors":"H. Dai, Huaijiang He, Xiu-hai Zhao, Chunyu Zhang, Jinsong Wang, Song Yang","doi":"10.3724/SP.J.1145.2013.00718","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00718","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
应用与环境生物学报Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00650
Hai-lin Ma, S. Xing, FangChun Liu, Bo Chen, Yanqin Ding, Yao Liang-tong, B. Du
{"title":"Screening and Identification of Three Plant Growth-promoting Rhizobacteria: Screening and Identification of Three Plant Growth-promoting Rhizobacteria","authors":"Hai-lin Ma, S. Xing, FangChun Liu, Bo Chen, Yanqin Ding, Yao Liang-tong, B. Du","doi":"10.3724/SP.J.1145.2013.00650","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00650","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69811204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}