Molekulyarnaya Biologiya最新文献_第3页

[Cytoplasmic mRNA Transport: Adaptors of mRNA Binding to Microtubule Motor Proteins]. 细胞质mRNA转运：mRNA与微管马达蛋白结合的接头。

Molekulyarnaya Biologiya Pub Date : 2024-05-01 DOI: 10.31857/S0026898424030018, EDN: JDPLWQ

Y A Vdovina, S G Georgieva, D V Kopytova

引用次数: 0

[Synthesis of a Bisbenzoxazole Analogue of Hoechst 33258 as a Potential GC-Selective DNA Ligand]. [双苯并恶唑类似物Hoechst 33258作为潜在gc -选择性DNA配体的合成]。

Molekulyarnaya Biologiya Pub Date : 2024-05-01 DOI: 10.31857/S0026898424030123, EDN: JCCURC

A F Arutyunyan, M S Aksenova, A A Kostyukov, A A Stomakhin, D N Kaluzhny, A L Zhuze

引用次数: 0

[What Actin and Myosin Do in the Nucleus: New Functions of the Well-Known Proteins]. [肌动蛋白和肌凝蛋白在细胞核中的作用：已知蛋白的新功能]。

Molekulyarnaya Biologiya Pub Date : 2024-05-01 DOI: 10.31857/S0026898424030029, EDN: JDMKVN

A A Saidova, I A Vorobjev

引用次数: 0

[Study of the Gut Transcriptomic Response in Drosophila melanogaster with Knockdown of Gagr, Domesticated gag Gene of Errantiviruses]. 黑腹果蝇肠道转录组反应的研究——敲低反病毒驯化gag基因

Molekulyarnaya Biologiya Pub Date : 2024-05-01 DOI: 10.31857/S0026898424030076, EDN: JCKOKP

M L Nikitina, P A Milyaeva, I V Kuzmin, L N Nefedova

{"title":"[Study of the Gut Transcriptomic Response in Drosophila melanogaster with Knockdown of Gagr, Domesticated gag Gene of Errantiviruses].","authors":"M L Nikitina, P A Milyaeva, I V Kuzmin, L N Nefedova","doi":"10.31857/S0026898424030076, EDN: JCKOKP","DOIUrl":"https://doi.org/10.31857/S0026898424030076, EDN: JCKOKP","url":null,"abstract":"As a result of molecular domestication of the gag gene of errantiviruses, the Gagr gene was formed in the genome of Drosophila melanogaster. It has previously been shown that the Gagr gene is transcribed at the highest level in gut tissues relative to other tissues, and its transcription is most effectively induced in females in response to ammonium persulfate added to the nutrient medium. In the present work, the gut transcriptome of females with knockdown of the Gagr gene was studied in all tissues under standard conditions and under stress conditions caused by ammonium persulfate. It was revealed that in females with knockdown of the Gagr gene, the genes of antimicrobial peptides controlled by the Toll and Imd signaling pathways are activated in the gut. Induction of a stress response by ammonium persulfate revealed disruption of the JAK/STAT and JNK/MAPK signaling pathways and an almost complete absence of activation of the ER-stress and UPR-stress pathways in flies with the Gagr gene knockdown. The data obtained confirm the important role of the Gagr gene in maintaining homeostasis and the immune response.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 3","pages":"425-436"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[ArdA Protein Specificity against Type I Restriction-Modification Systems]. [ArdA蛋白对I型限制性修饰系统的特异性]。

Molekulyarnaya Biologiya Pub Date : 2024-05-01 DOI: 10.31857/S0026898424030107, EDN: JCEPFL

A A Kudryavtseva, A V Vlasov, E V Zinovev, D D Yanovskaya, A A Utkina, S M Rastorguev, I V Manukhov

引用次数: 0

[Uveal Melanoma: Molecular and Genetic Mechanisms of Development and Therapeutic Approaches]. [葡萄膜黑色素瘤：发展的分子和遗传机制及治疗方法]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020017, EDN: NQWJSC

M V Zhilnikova, O S Troitskaya, D D Novak, V V Atamanov, O A Koval

{"title":"[Uveal Melanoma: Molecular and Genetic Mechanisms of Development and Therapeutic Approaches].","authors":"M V Zhilnikova, O S Troitskaya, D D Novak, V V Atamanov, O A Koval","doi":"10.31857/S0026898424020017, EDN: NQWJSC","DOIUrl":"https://doi.org/10.31857/S0026898424020017, EDN: NQWJSC","url":null,"abstract":"Uveal melanoma (UM) is a neuroectodermal tumor that results from malignant transformation of melanocytes in the eye uvea, including the iris, the ciliary body, and the choroid. UM accounts for 5% of all melanoma cases and is extremely aggressive with half of the UM patients developing metastases within the first 1-2 years after tumor development. Molecular mechanisms of UM carcinogenesis are poorly understood, but are known to differ from those of skin melanoma. Activating mutations of the GNAQ and GNA11 genes, which code for the large G protein subunits Gq and G11, respectively, are found in 90% of UM patients. The Gaq/PKC/MAPK signaling pathway is a main signaling cascade that leads to the transformation of melanocytes of the uveal tract, and major regulators of the cascade provide targets for the development of drugs. Metastatic UM (MUM) is most often associated with mutations of BAP1, EIF1AX, GNA11, GNAQ, and SF3B1. A combination of a commercial expression test panel of 15 genes and a mutation panel of 7 genes, supplemented with data on the size of the primary tumor, is highly efficient in predicting the risk of metastasis. The risk of metastasis determines the choice of therapy and the patient follow-up regimen. However, no systemic therapy for MUM has been developed to date. New drugs undergoing clinical trials are mostly targeted drugs designed to inhibit the protein products of mutant genes or immunotherapeutic agents designed to stimulate the immune response against specific antigens. In addition to these approaches, potential therapeutic targets of epigenetic regulation of UM development are considered in the review.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 2","pages":"189-203"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Method of Inducible Knockdown of Essential Genes in OSC Cell Culture of Drosophila melanogaster]. [黑腹果蝇 OSC 细胞培养中重要基因的诱导性敲除方法]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020137, EDN: NDBKYZ

S V Marfina, E A Mikhaleva, N V Akulenko, S S Ryazansky

引用次数: 0

[The Structural Features of Skeletal Muscle Titin Aggregates]. [骨骼肌 Titin 聚合体的结构特征]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020143, EDN: MYWWYU

L G Bobyleva, T A Uryupina, N V Penkov, M A Timchenko, A D Ulanova, A G Gabdulkhakov, I M Vikhlyantsev, A G Bobylev

{"title":"[The Structural Features of Skeletal Muscle Titin Aggregates].","authors":"L G Bobyleva, T A Uryupina, N V Penkov, M A Timchenko, A D Ulanova, A G Gabdulkhakov, I M Vikhlyantsev, A G Bobylev","doi":"10.31857/S0026898424020143, EDN: MYWWYU","DOIUrl":"https://doi.org/10.31857/S0026898424020143, EDN: MYWWYU","url":null,"abstract":"Titin is a multidomain protein of striated and smooth muscles of vertebrates. The protein consists of repeating immunoglobulin-like (Ig) and fibronectin-like (FnIII) domains, which are β-sandwiches with a predominant β-structure, and also contains disordered regions. In this work, the methods of atomic force microscopy (AFM), X-ray diffraction, and Fourier transform infrared spectroscopy were used to study the morphology and structure of aggregates of rabbit skeletal muscle titin obtained in two different solutions: 0.15 M glycine-KOH, pH 7.0 and 200 mM KCl, 10 mM imidazole, pH 7.0. According to AFM data, skeletal muscle titin formed amorphous aggregates of different morphologies in the above two solutions. Amorphous aggregates of titin formed in a solution containing glycine consisted of much larger particles than aggregates of this protein formed in a solution containing KCl. The \"KCl-aggregates\" according to AFM data had the form of a \"sponge\"-like structure, while amorphous \"glycine-aggregates\" of titin formed \"branching\" structures. Spectrofluorometry revealed the ability of \"glycine-aggregates\" of titin to bind to the dye thioflavin T (TT), and X-ray diffraction revealed the presence of one of the elements of the amyloid cross β-structure, a reflection of ~4.6 Å, in these aggregates. These data indicate that \"glycine-aggregates\" of titin are amyloid or amyloid-like. No similar structural features were found in \"KCl-aggregates\" of titin; they also did not show the ability to bind to thioflavin T, indicating the non-amyloid nature of these titin aggregates. Fourier transform infrared spectroscopy revealed differences in the secondary structure of the two types of titin aggregates. The data we obtained demonstrate the features of structural changes during the formation of intermolecular bonds between molecules of the giant titin protein during its aggregation. The data expand the understanding of the process of amyloid protein aggregation.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 2","pages":"314-324"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology]. [正常和病理学中 RNA 聚合酶 III 启动子对转录的调控]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020032, EDN: NNFJKS

A M Schwartz, K A Tatosyan, D V Stasenko, D A Kramerov

{"title":"[Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology].","authors":"A M Schwartz, K A Tatosyan, D V Stasenko, D A Kramerov","doi":"10.31857/S0026898424020032, EDN: NNFJKS","DOIUrl":"https://doi.org/10.31857/S0026898424020032, EDN: NNFJKS","url":null,"abstract":"RNA polymerase III synthesizes a wide range of noncoding RNAs shorter than 400 nucleotides in length. These RNAs are involved in protein synthesis (tRNA, 5S rRNA, and 7SL RNA), maturation, and splicing of different types of RNA (RPR, MRP RNA, and U6 snRNA), regulation of transcription (7SK RNA), replication (Y RNA), and intracellular transport (vault RNA). BC200 and BC1 RNA genes are transcribed by RNA polymerase III in neurons only where these RNAs regulate protein synthesis. Mutations in the regulatory elements of the genes transcribed by RNA polymerase III as well as in transcription factors of this RNA polymerase are associated with the development of a number of diseases, primarily oncological and neurological. In this regard, the mechanisms of regulation of the expression of the genes containing various RNA polymerase III promoters were actively studied. This review describes the structural and functional classification of polymerase III promoters, as well as the factors involved in the regulation of promoters of different types. A number of examples demonstrate the role of the described factors in the pathogenesis of human diseases.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 2","pages":"220-233"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Changes in the Genome of the Tick-Borne Encephalitis Virus during Cultivation]. [蜱传脑炎病毒基因组在培养过程中的变化]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020093, EDN: NILAFS

V A Ternovoi, E P Ponomareva, E V Protopopova, N L Tupota, T P Mikryukova, V B Loktev

{"title":"[Changes in the Genome of the Tick-Borne Encephalitis Virus during Cultivation].","authors":"V A Ternovoi, E P Ponomareva, E V Protopopova, N L Tupota, T P Mikryukova, V B Loktev","doi":"10.31857/S0026898424020093, EDN: NILAFS","DOIUrl":"https://doi.org/10.31857/S0026898424020093, EDN: NILAFS","url":null,"abstract":"The tick-borne encephalitis virus (TBEV) strain C11-13 (GenBank acc. no. OQ565596) of the Siberian genotype was previously isolated from the brain of a deceased person. TBEV C11-13 variants obtained at passages 3 and 8 in SPEV cells were inoculated into the brains of white mice for subsequent passages. Full genome sequences of all virus variants were analyzed by high-throughput sequencing. A total of 41 single nucleotide substitutions were found to occur mainly in the genes for the nonstructural proteins NS3 and NS5 (GenBank MF043953, OP902894, and OP902895), and 12 amino acid substitutions were identified in the deduced protein sequences. Reverse nucleotide and amino acid substitutions were detected after three passages through mouse brains. The substitutions restored the primary structures that were characteristic of the isolate C11-13 from a human patient and changed during the eight subsequent passages in SPEV cells. In addition, the 3'-untranslated region (3'-UTR) of the viral genome increased by 306 nt. The Y3 and Y2 3'-UTR elements were found to contain imperfect L and R repeats, which were probably associated with inhibition of cellular XRN1 RNase and thus involved in the formation of subgenomic flaviviral RNAs (sfRNAs). All TBEV variants showed high-level reproduction in both cell cultures and mouse brains. The genomic changes that occurred during successive passages of TBEV are most likely due to its significant genetic variability, which ensures its efficient reproduction in various hosts and its broad distribution in various climatic zones.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 2","pages":"282-294"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0