Molekulyarnaya Biologiya最新文献_第3页

[Marine Fungi: In Search of New Antibacterial Drugs]. [海洋真菌：寻找新的抗菌药物]。

Molekulyarnaya Biologiya Pub Date : 2025-01-01 DOI: 10.31857/S0026898425010041, EDN: HDESIP

E A Yurchenko, E A Chingizova, D L Aminin, A N Yurchenko

引用次数: 0

[Mechanism of Thiocyanate Dehydrogenase Functioning Based on Structural Data]. [基于结构数据的硫氰酸脱氢酶作用机理]。

Molekulyarnaya Biologiya Pub Date : 2025-01-01 DOI: 10.31857/S0026898425010103, EDN: HCMHTC

K M Polyakov, S Gavryushov

{"title":"[Mechanism of Thiocyanate Dehydrogenase Functioning Based on Structural Data].","authors":"K M Polyakov, S Gavryushov","doi":"10.31857/S0026898425010103, EDN: HCMHTC","DOIUrl":"10.31857/S0026898425010103, EDN: HCMHTC","url":null,"abstract":"Thiocyanate dehydrogenase is enzyme catalyzing transformation of a thiocyanate ion into a cyanate ion with outcome of two electrons, two protons and a neutral atom of sulphur. Earlier structures of thiocyanate dehydrogenase from Thioalkalivibrio paradoxus were solved. Despite not perfect quality of the structures (twinning and pronounced anisotropy of the crystals, incomplete occupancy of the copper ions, absence of data for complexes with analogues of the substrate), there was suggested a mechanism of the enzyme functioning based on those structures. Recently at atomic resolution there have been solved structures of a gene-modified copy of relative enzyme from Ptlomicrobium methylotrophicum for free protein and its complex with thiourea. In the new structures copper ions of the active site possess complete occupancy. In these structures it is possible to reliably identify two conformations of the protein molecule with opened and closed active sites. The new structural high resolution data also allowed us to determine the presence of the superposition of different states of the copper ions for each of the two conformations. In each state the copper ions have different oxidation degrees, different corresponding ligands and partial occupancies. The ion charges were determined according to the ions coordination. In the protein molecule with the closed active site the complexes with inhibitor (thiourea ion) and molecular oxygen are observed. The complex with thiourea allows us to model binding of thiocyanate ion to the enzyme molecule. Taking into account the changes of the structures in the opened and closed conformations, a mechanism of the attacking oxygen ligand activation is suggested. A new scheme of the enzymatic reaction is discussed.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"59 1","pages":"141-153"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Substrate Efficiency of Cy5-Modified Derivatives of Deoxyuridine and Deoxycytidine in the Rolling Circle Amplification]. [cy5修饰的脱氧尿嘧啶和脱氧胞苷衍生物在滚圈扩增中的底物效率]。

Molekulyarnaya Biologiya Pub Date : 2025-01-01 DOI: 10.31857/S0026898425010086, EDN: HCQMZQ

P A Chirkova, S A Surzhikov, V E Kuznetsova, V E Shershov, A V Chudinov, S A Lapa

引用次数: 0

[NOS1AP Gene Polymorphism and Body Fat in Patients with Schizophrenia: A Search for Associations]. NOS1AP基因多态性与精神分裂症患者体脂的相关性研究

Molekulyarnaya Biologiya Pub Date : 2025-01-01 DOI: 10.31857/S0026898425010073, EDN: HCZVBF

V V Tiguntsev, I A Mednova, D A Petkun, A A Agarkov, A N Kornetov, I V Pozhidaev, D Z Paderina, E G Kornetova, S A Ivanova

{"title":"[NOS1AP Gene Polymorphism and Body Fat in Patients with Schizophrenia: A Search for Associations].","authors":"V V Tiguntsev, I A Mednova, D A Petkun, A A Agarkov, A N Kornetov, I V Pozhidaev, D Z Paderina, E G Kornetova, S A Ivanova","doi":"10.31857/S0026898425010073, EDN: HCZVBF","DOIUrl":"10.31857/S0026898425010073, EDN: HCZVBF","url":null,"abstract":"Long-term pharmacotherapy in patients with schizophrenia can provoke antipsychotic-induced obesity. This side effect does not always meet the criteria for metabolic syndrome (MS), primarily central obesity. However, this significantly reduces the quality of life of patients and is a risk factor for the development of many diseases. In humans, the NOS1AP gene product is involved in adipogenesis, dendrite maturation, mnemonic processes, and impulse transmission by NMDA receptors. We hypothesized that NOS1AP gene polymorphisms are associated with metabolic parameters in patients with schizophrenia. We examined 491 patients of Slavic nationalities with an established diagnosis of schizophrenia. All participants underwent anthropometric examination to determine waist circumference and the total and visceral fat content using bioimpedance analysis and caliperometry. The biochemical parameters of the blood serum were evaluated by standard methods. MS components were determined according to the International Diabetes Federation criteria. DNA was isolated from peripheral blood leukocytes by the standard phenol-chloroform method. Three SNPs in the NOS1AP gene were selected for genotyping. The alleles of the studied polymorphisms were determined by real-time PCR. As a result, statistically significant differences in the groups of patients with different levels of visceral fat in the distribution of the allele frequency of the s12143842 NOS1AP polymorphism, as well as differences in the levels of visceral fat depending on the rs10494366 NOS1AP genotype were revealed. For the first time, an association of NOS1AP gene polymorphisms with the formation of visceral fat levels in patients with schizophrenia was established. The results obtained can be further used to develop genetic panels to predict the development of adverse metabolic effects during antipsychotic therapy for schizophrenia.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"59 1","pages":"117-125"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Autophagy Impairment in Parkinson's Disease: Approaches to Therapy]. [帕金森病自噬损伤：治疗方法]。

Molekulyarnaya Biologiya Pub Date : 2025-01-01 DOI: 10.31857/S0026898425010053, EDN: HDEMSA

T S Usenko

{"title":"[Autophagy Impairment in Parkinson's Disease: Approaches to Therapy].","authors":"T S Usenko","doi":"10.31857/S0026898425010053, EDN: HDEMSA","DOIUrl":"10.31857/S0026898425010053, EDN: HDEMSA","url":null,"abstract":"Parkinson's disease (PD) is one of the most common neurodegenerative disorders and is characterized by progressive motor impairment due to the death of dopaminergic neurons in the substantia nigra (SN) of the brain. PD affects more than 1% of the population over 60 years of age worldwide. Despite significant progress in understanding the pathogenesis of PD, including genetic and biochemical aspects, current therapy is limited to symptomatic treatments. Recent evidence suggests that impaired autophagy leads to the accumulation of abnormal proteins and, particularly, α-synuclein, aggregated forms of which are neurotoxic to dopaminergic neurons in the SN. Notably, PD is predominantly sporadic. However, monogenic PD forms have also been described. PD forms associated with mutations of the GBA1 or LRRK2 gene are among the most common PD forms with known etiology. Leucine-rich repeat kinase 2 (LRRK2), which is encoded by LRRK2, and the lysosomal enzyme glucocerebrosidase (GCase), which is encoded by GBA1, are involved in the same endolysosomal pathway. LRRK2 and GCase dysfunction reported in PD, especially in cases with mutations of the respective genes, can impair the endolysosomal pathway, the lysosomal function, and possibly autophagy. The review highlights the molecular mechanisms of autophagy and the prospects for targeted therapy of PD via induction of autophagy by influencing the key players in the process.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"59 1","pages":"60-79"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[CpG Traffic Lights Are Involved in Active DNA Demethylation]. [CpG交通灯参与活性DNA去甲基化]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060122, EDN: IAHHQG

A V Lioznova, Yu A Medvedeva

{"title":"[CpG Traffic Lights Are Involved in Active DNA Demethylation].","authors":"A V Lioznova, Yu A Medvedeva","doi":"10.31857/S0026898424060122, EDN: IAHHQG","DOIUrl":"10.31857/S0026898424060122, EDN: IAHHQG","url":null,"abstract":"DNA methylation is one of the most important mechanisms closely involved in the epigenetic regulation of gene expression. However, the relationship between DNA methylation and expression is not completely understood. There are reported examples of changes in DNA methylation being the cause of gene expression, and vice versa - examples of changes in gene expression to entail changes in methylation. Earlier, we introduced the concept of CpG traffic lights - individual CpG sites methylation levels of which significantly correlate with the expression of a gene nearby - and showed their important role in the regulation of enhancers. In this work, we show that the levels of CpG traffic lights methylation are heterogeneous in the cell population, and suggest that this feature is caused by their dynamic demethylation. The 5hmC enrichment and TET2 localization sites in CpG traffic lights confirm our hypothesis. In order to find out whether methylation of CpG sites is the cause or the consequence of the gene expression, we applied the assessment of the causation direction method developed by Jonas Peters and co-authors. We determined that in CpG sites where changes in methylation are predicted to be the cause of gene expression changes (M→E CpG sites), methylation levels are more stable in different cells and active demethylation occurs less often than in CpG sites where presumably changes in gene expression cause changes in methylation (E→M CpG sites). We also show a greater share of M→E CpG sites in the promoter regions than in the bodies of the genes where E→M CpG sites are more common. Based on these observations, we believe that methylation of M→E CpG sites is stable and functions as an \"on/off\" switch. On the contrary methylation of E→M sites is dynamic, variable between cells in a cell population, and is caused primarily by active demethylation.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"1020-1030"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of the Clinical Isolate CCGC 19/16 as Bacillus cytotoxicus]. 临床分离物CCGC 19/16为细胞毒性芽孢杆菌的鉴定

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060099, EDN: IASZNL

N B Polyakov, D S Karpov, M V Zubasheva, A N Polyakova, D N Shcherbinin, A I Solovyev, M V Lavrentiev, T A Smirnova, M A Sukhina, V G Zhukhovitsky

{"title":"[Identification of the Clinical Isolate CCGC 19/16 as Bacillus cytotoxicus].","authors":"N B Polyakov, D S Karpov, M V Zubasheva, A N Polyakova, D N Shcherbinin, A I Solovyev, M V Lavrentiev, T A Smirnova, M A Sukhina, V G Zhukhovitsky","doi":"10.31857/S0026898424060099, EDN: IASZNL","DOIUrl":"10.31857/S0026898424060099, EDN: IASZNL","url":null,"abstract":"Bacillus cereus sensu lato comprises genetically, morphologically, and physiologically similar gram-positive spore-forming bacterial species with high pathogenic potential, such as B. anthracis, B. cereus, and B. thuringiensis. Toxin-producing strains of B. cereus s.I. pose a major threat to human health. The high degree of similarity between these species makes it very difficult to identify them and to take adequate measures to treat the diseases they cause. Previously, we characterized the clinical isolate CCGC 19/16 belonging to B. cereus s.l. that exhibited features of both B. cereus and B. cytotoxicus. In the present work, CCGC 19/16 was identified as B. cytotoxicus using multilocus sequence typing (MLST) and mass spectrometric analysis. It was also shown that, unlike other representatives of the B. cytotoxicus species, strain CCGC 19/16 is not thermotolerant. Unlike B. cereus, strain CCGC 19/16 is sensitive to most antibiotics and shows increased motility. Like B. cereus strain CCGC 19/16 forms β-hemolysis zones in blood agar. In addition, it has been shown that prolonged storage of samples prior to analysis can lead to misidentification of the isolate. Our results indicate that \"rapid methods\" of analysis using single genes have insufficient resolving power in the identification of B. cereus s.l. species. The combination of MLST analysis with MALDI-TOF MS provides sufficient resolution.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"983-995"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of a New Inhibitor of Bacterial Cystathionine γ-Lyase Based on 6-Bromoindole and Aminothiophene]. [基于6-溴吲哚和氨基噻吩的新型细菌胱硫氨酸γ-裂解酶抑制剂的研制]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060083, EDN: IAYTTU

R A Novikov, D N Platonov, A Yu Belyy, K V Potapov, M A Novikov, Yu V Tomilov, O I Kechko, T A Seregina, P N Solyev, V A Mitkevich

引用次数: 0

[Distribution of β-Lactamase-Producing Enterobacterales among Patients in Surgical and Therapeutic Departments of a Multidisciplinary Hospital]. [某多学科医院外科及治疗科产β-内酰胺酶肠杆菌的分布]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060115, EDN: IAJXDD

S Yu Meshchurova, A G Korobova, L M Samokhodskaya

{"title":"[Distribution of β-Lactamase-Producing Enterobacterales among Patients in Surgical and Therapeutic Departments of a Multidisciplinary Hospital].","authors":"S Yu Meshchurova, A G Korobova, L M Samokhodskaya","doi":"10.31857/S0026898424060115, EDN: IAJXDD","DOIUrl":"10.31857/S0026898424060115, EDN: IAJXDD","url":null,"abstract":"Adequate empiric therapy is difficult to choose without monitoring the local distribution of antibiotic-resistant bacteria in each hospital. The frequency of β-lactamase-producing Enterobacterales was compared in patients of therapeutic and surgical units. Antibiotic susceptibility was evaluated by the disk diffusion test. Production of extended-spectrum β-lactamases (ESBLs) was detected by the double-disk test, and carbapenemases were determined by a modified carbapenem inactivation method. Carbapenemase genes and their expression were quantified by real-time PCR and immunochromatography. More than one-third of Enterobacterales isolates produced ESBLs in both the therapeutic (35.51%) and surgical (39.85%) units. The proportions of carbapenemase producers was comparable in the two groups, amounting to 8.41 and 9.77%, respectively. Metallo-β-lactamases predominated in the surgical units; and serine β-lactamases, in the therapeutic units. β-Lactamase producers were less frequent among community-acquired isolates than among nosocomial ones in both therapeutic (31.48 and 56.6%) and surgical (45.45 and 51%) units, but the differences were nonsignificant. Although the proportion of β-lactamase producers in the surgical and therapeutic units was not found to increase over three years of the study, local monitoring should certainly be continued in order to develop a local strategy for adequate use of antibacterial drugs.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"1007-1019"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antibiotic Resistance: Threats and Search for Solution]. [抗生素耐药性：威胁与解决方案的探索]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060012, EDN: HNDNRK

S N Kochetkov

引用次数: 0