Journal of Medical and Dental Sciences最新文献_第9页

Efficacy of lidocaine lontophoresis using either alternating or direct current in hairless rats. 交流电或直流电利多卡因对无毛大鼠lontopresis的影响。

Journal of Medical and Dental Sciences Pub Date : 2013-09-30

Atsushi Nakajima, Ryo Wakita, Haruka Haida, Haruhisa Fukayama

{"title":"Efficacy of lidocaine lontophoresis using either alternating or direct current in hairless rats.","authors":"Atsushi Nakajima, Ryo Wakita, Haruka Haida, Haruhisa Fukayama","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to determine transport of lidocaine ions through a hairless rat skin in vivo and to compare the efficacy of alternating current (AC) with that of direct current (DC) iontophoresis (IOP). We measured the concentration of lidocaine transported through a cellophane membrane or a hairless rat dorsal skin applying either AC-IOP or DC-IOP. The results revealed that lidocaine concentration increased in a time-dependent manner in vitro in both DC-IOP and AC-IOP. However, the in vivo study showed different tendencies in lidocaine concentration. In the DCIOP group, lidocaine concentration reached its maximum 20 min after current application and then decreased rapidly; the AC-IOP group showed an increase in lidocaine concentration in a time-dependent manner. There were no side effects such as electrical burns in the rats. In conclusion, AC can be applied for long periods and DC for short periods, or their application time can be appropriately scheduled. Our study also suggests the mechanism by which voltage waveforms affect the skin when applied by IOP. In the future, these findings will be a solid foundation for developing various kinds of medical equipment such as scheduled drug delivery system that can easily deliver various types of drug.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 3","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40254724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic subdural hematoma - an up-to-date concept. 慢性硬膜下血肿-一个最新的概念。

Journal of Medical and Dental Sciences Pub Date : 2013-06-01

Yoji Tanaka, Kikuo Ohno

引用次数: 0

Chronic subdural hematoma - an up-to-date concept. 慢性硬膜下血肿-一个最新的概念。

Journal of Medical and Dental Sciences Pub Date : 2013-06-01 DOI: 10.11480/JMDS.600201

Yoji Tanaka, K. Ohno

引用次数: 39

Efficient in vivo delivery of antisense oligonucleotide to choroid plexus. 反义寡核苷酸在脉络膜丛体内的高效递送。

Journal of Medical and Dental Sciences Pub Date : 2013-03-01 DOI: 10.11480/JMDS.600102

W. Piao, K. Nishina, K. Yoshida-Tanaka, H. Kuwahara, Tomoko Nishina, Mina Sakata, H. Mizusawa, T. Yokota

{"title":"Efficient in vivo delivery of antisense oligonucleotide to choroid plexus.","authors":"W. Piao, K. Nishina, K. Yoshida-Tanaka, H. Kuwahara, Tomoko Nishina, Mina Sakata, H. Mizusawa, T. Yokota","doi":"10.11480/JMDS.600102","DOIUrl":"https://doi.org/10.11480/JMDS.600102","url":null,"abstract":"The choroid plexus (CP) is present on the ventricular walls of the brain, produces cerebrospinal fluid (CSF), contains many blood vessels, and is a major functional component of the blood-CSF barrier. The CP is an important site in the pathophysiology of various neurological diseases, including Alzheimer's disease and meningeal amyloidosis. We performed gene silencing in the CP in vivo by using an antisense oligonucleotide (ASO). A short ASO of length 12 nucleotides was intravenously injected into rats. The ASO was not delivered to neurons or glia in the central nervous system, but was successfully delivered into the CP, and resulted in a significant reduction of endogenous target gene expression in epithelial cells within the CP. Although the mechanism of uptake of the ASO by the CP was not elucidated, the ASO bound to albumin in vivo, and the distribution of ASO delivery was similar to that of albumin delivery. These findings suggest that we inhibited target gene expression in the epithelial cells of the CP via albumin-ASO conjugates. This strategy should be useful for investigations of the function of CP, and for the development of new gene-silencing therapies for diseases with pathophysiology related to the CP.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom. 无LQT2疾病相关症状的KCNH2 G603D患者诱导多能干细胞的产生

Journal of Medical and Dental Sciences Pub Date : 2013-03-01

Shinichiro Okata, Shinsuke Yuasa, Teiichi Yamane, Tetsushi Furukawa, Keiichi Fukuda

{"title":"The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom.","authors":"Shinichiro Okata, Shinsuke Yuasa, Teiichi Yamane, Tetsushi Furukawa, Keiichi Fukuda","doi":"","DOIUrl":"","url":null,"abstract":"The long QT syndrome type 2 (LQT2) is inheritable life threatening arrhythmic disorder and one of the most common genetic variants in long QT syndrome. There are some indications for treatment of the patients with LQT2 but it is impossible to completely prevent fatal arrhythmia. To develop novel therapy for the patients with LQT2, it has been desired to generate diseasespecific and patient-specific disease model. Human induced pluripotent stem (iPS) cells are somatic cell-derived pluripotent stem cells with infinite proliferation ability and multipotency. Patient-specific iPS cells can be derived from patient somatic cells, have all genomic information encoded in patient's genome including mutation and all SNPs, and can be ideal disease models of the patients. To generate disease model for LQT2 by iPS cells, we should firstly generate iPS cells from the patient with LQT2 and confirm the genomic mutation in iPS cells. In this study, we showed the successful generation of iPS cells from a patient with KCNH2 G603D mutation. The patient specific iPS cells properly expressed stem cell markers, such as NANOG and OCT3/4. We also confirmed that the KCNH2 G603D (G1808A) mutation was taken over in patient specific iPS cells. These patient-specific iPS cells may contribute to the future analysis for disease pathogenesis and drug innovation. ","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31635506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between doctor's belief on the patient's self-determination and medical outcomes in obtaining informed consent. 医生对病人自决的信念与获得知情同意的医疗结果之间的相关性。

Journal of Medical and Dental Sciences Pub Date : 2013-03-01 DOI: 10.11480/JMDS.600104

Keisuke Yoshihara, K. Takase

{"title":"Correlation between doctor's belief on the patient's self-determination and medical outcomes in obtaining informed consent.","authors":"Keisuke Yoshihara, K. Takase","doi":"10.11480/JMDS.600104","DOIUrl":"https://doi.org/10.11480/JMDS.600104","url":null,"abstract":"We employed a questionnaire survey to assess attitudes toward informed consent (IC) among hospital doctors. Based on the result of the correlation analysis, the following two hypotheses were identified. The first hypothesis is that \"the doctor's belief that the patient's self-determination is possible promotes cure of illness by obtaining IC.\" The second hypothesis is that \"the doctor's belief that the patient's self-determination is possible has a positive influence on patient's quality of life by obtaining IC.\" We clarified the rationale for explaining these two hypotheses by applying cross tabulation analysis, discriminant analysis and principal component analysis (PCA). The doctors were divided into two groups in terms of their position on the patient's self-determination. One group of doctors believed the possibility of patient's self-determination, and the other did not. Through our statistical analyses, the characteristics that discriminate these two groups were identified. It was revealed that the former group placed a great importance on the hospitality value, while the latter placed an importance on the service value. Agreement or rejection of the concept of IC has been demonstrated as a key distinguishing factor between the two groups. The results of PCA showed that the doctor's belief on the patient's self-determination in obtaining IC had a significant effect on medical outcomes, and the two above-mentioned hypotheses were revealed.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1 1","pages":"23-40"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The pulmonary tissue damage associated with the aspiration of gelatinizers in rats. 大鼠吸入胶凝剂对肺组织的损伤。

Journal of Medical and Dental Sciences Pub Date : 2013-03-01 DOI: 10.11480/JMDS.600101

Ritsu Nishimura, Naoto Sugiyama, I. Fujishima

{"title":"The pulmonary tissue damage associated with the aspiration of gelatinizers in rats.","authors":"Ritsu Nishimura, Naoto Sugiyama, I. Fujishima","doi":"10.11480/JMDS.600101","DOIUrl":"https://doi.org/10.11480/JMDS.600101","url":null,"abstract":"Various gelatinizers, which facilitate oral ingestion, are employed in patients with dysphagia. The purpose of this study was to histologically clarify the influence of various gelatinizers on the lung, using rats. We administered 0.2 ml/kg of 0.1% xanthangam, a 0.25% commercially available xanthangam gelatinizer, 0.35% ι-carrageenan, 0.5% κ-carrageenan, 1% gelatin, 0.15% agar, physiological saline, tap water, and isopropanolpurified 0.1% xanthangam/0.35% ι-carrageenan into the trachea of 8- to 9-week-old male SD rats. The lungs were extirpated after 24 and 72 hours. Neutrophil infiltration in the alveolar space was expressed as the mean number of neutrophils in 30 randomly selected high-power fields. In the xanthangam (451.0 ± 204.0 cells) -, and the ι -carrageenan (424.4 ± 257.2) treated groups, the neutrophil counts after 24 hours was significantly greater than in the physiological saline (33.0 ± 22.6) - treated group (p < 0.05). In the available xanthangam gelatinizer (290.0 ± 86.8) -treated group was no significant difference in the physiological saline-treated group. In the isopropanol-purified xanthangam (90.2 ± 42.3)-treated group, the neutrophil counts after 24 hours were significantly smaller than in the nonpurified xanthangam -treated group.These results suggest that lung tissue inflammatory response-inducing features depend on the type of gelatinizer. On the other hand, purification reduces the lung-damaging features of xanthangam.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Changes in the psychological characteristics of oral cancer patients in the perioperative period: a quantitative evaluation. 口腔癌患者围手术期心理特征变化的定量评价。

Journal of Medical and Dental Sciences Pub Date : 2013-03-01

Aya Koizumi, Eisuke Matsushima, Yumi Mochizuki, Ken Omura, Teruo Amagasa

{"title":"Changes in the psychological characteristics of oral cancer patients in the perioperative period: a quantitative evaluation.","authors":"Aya Koizumi, Eisuke Matsushima, Yumi Mochizuki, Ken Omura, Teruo Amagasa","doi":"","DOIUrl":"","url":null,"abstract":"We examined the changes in psychological distress and quality of life (QOL) during the perioperative period in oral cancer patients undergoing surgery and investigated the relationship between patient's psychological distress and QOL. Methods. Fifty patients participated. The Hospital Anxiety and Depression Scale (HADS; Japanese version), as a psychological test and the Functional Assessment of Cancer Therapy General (FACT-G); and Head and Neck (FACT-H&N), as quality of life (QOL) surveys were administered preoperatively, after surgery, and 1 month after leaving the hospital. Results. Anxiety was highest pre-operation and depression was highest post-operation, but improvements in both were seen post-discharge. At the pre-operation time point, anxiety and depression low-score groups had significantly high scores on Emotional well-being and Functional well-being. At the post-operation time point, anxiety and depression low-score groups had significantly high scores on all QOL subscales. Conclusion. Providing psychological support while considering anxiety might be particularly useful preoperatively whereas providing psychological support while considering depression might be particularly useful postoperatively. ","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1","pages":"41-53"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31635508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom. 无LQT2疾病相关症状的KCNH2 G603D患者诱导多能干细胞的产生

Journal of Medical and Dental Sciences Pub Date : 2013-03-01 DOI: 10.11480/JMDS.600103

Shinichiro Okata, S. Yuasa, T. Yamane, T. Furukawa, K. Fukuda

{"title":"The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom.","authors":"Shinichiro Okata, S. Yuasa, T. Yamane, T. Furukawa, K. Fukuda","doi":"10.11480/JMDS.600103","DOIUrl":"https://doi.org/10.11480/JMDS.600103","url":null,"abstract":"The long QT syndrome type 2 (LQT2) is inheritable life threatening arrhythmic disorder and one of the most common genetic variants in long QT syndrome. There are some indications for treatment of the patients with LQT2 but it is impossible to completely prevent fatal arrhythmia. To develop novel therapy for the patients with LQT2, it has been desired to generate diseasespecific and patient-specific disease model. Human induced pluripotent stem (iPS) cells are somatic cell-derived pluripotent stem cells with infinite proliferation ability and multipotency. Patient-specific iPS cells can be derived from patient somatic cells, have all genomic information encoded in patient's genome including mutation and all SNPs, and can be ideal disease models of the patients. To generate disease model for LQT2 by iPS cells, we should firstly generate iPS cells from the patient with LQT2 and confirm the genomic mutation in iPS cells. In this study, we showed the successful generation of iPS cells from a patient with KCNH2 G603D mutation. The patient specific iPS cells properly expressed stem cell markers, such as NANOG and OCT3/4. We also confirmed that the KCNH2 G603D (G1808A) mutation was taken over in patient specific iPS cells. These patient-specific iPS cells may contribute to the future analysis for disease pathogenesis and drug innovation.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Correlation between doctor's belief on the patient's self-determination and medical outcomes in obtaining informed consent. 医生对病人自决的信念与获得知情同意的医疗结果之间的相关性。

Journal of Medical and Dental Sciences Pub Date : 2013-03-01

Keisuke Yoshihara, Kozo Takase

{"title":"Correlation between doctor's belief on the patient's self-determination and medical outcomes in obtaining informed consent.","authors":"Keisuke Yoshihara, Kozo Takase","doi":"","DOIUrl":"","url":null,"abstract":"We employed a questionnaire survey to assess attitudes toward informed consent (IC) among hospital doctors. Based on the result of the correlation analysis, the following two hypotheses were identified. The first hypothesis is that \"the doctor's belief that the patient's self-determination is possible promotes cure of illness by obtaining IC.\" The second hypothesis is that \"the doctor's belief that the patient's self-determination is possible has a positive influence on patient's quality of life by obtaining IC.\" We clarified the rationale for explaining these two hypotheses by applying cross tabulation analysis, discriminant analysis and principal component analysis (PCA). The doctors were divided into two groups in terms of their position on the patient's self-determination. One group of doctors believed the possibility of patient's self-determination, and the other did not. Through our statistical analyses, the characteristics that discriminate these two groups were identified. It was revealed that the former group placed a great importance on the hospitality value, while the latter placed an importance on the service value. Agreement or rejection of the concept of IC has been demonstrated as a key distinguishing factor between the two groups. The results of PCA showed that the doctor's belief on the patient's self-determination in obtaining IC had a significant effect on medical outcomes, and the two above-mentioned hypotheses were revealed. ","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 1","pages":"23-40"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31635507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0