Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA最新文献

{"title":"Hepatitis B virus: a new platform for in vitrostudies of infection, replication cycle and pathogenesis","authors":"Carla Rios da Cruz, Rute Lopes, M. Santana, P. I. Costa","doi":"10.4322/2179-443X.0671","DOIUrl":"https://doi.org/10.4322/2179-443X.0671","url":null,"abstract":"Hepatitis B virus (HBV) research has been hampered by the lack of suitable and reproducible cell culture systems that reliably mimic the viral life cycle. Several infection stages and metabolic aspects related to HBV cycle still need to be elucidated. The aim of this research was the study of techniques aiming at developing a sustainable in vitro platform of hepatitis B virus infection, in a simplified and low maintenance approach, evaluating the continuity of infection in cell culture throughout many passages, using a positive serum pool to the virus in human hepatocellular carcinoma. The viral load was quantified by real-time polymerase chain reaction. The cells underwent a freeze and thaw cycle, followed by seeding, and the new culture was analyzed to quantify the viral load. An aliquot was used to detect the surface antigen (HBsAg), by chemiluminescence. The detection of the core antigen (HBcAg) was performed by flow cytometry and by immunofluorescence microscopy. Viral load remained detectable throughout the studied period, 50 days after initial infection. The process of freezing and seeding produced detectable viral load for a 7-day period. HBsAg was reagent in the infected cells, confirming the maintenance of infection. The flow cytometry result indicated 11.85% of HBcAg positive cells, which demonstrates that new viral particles were at the assembly stage. Indirect immunofluorescence using epiluminescence microscopy allowed the detection of viral HBcAg in the interior of infected cells, confirming the results obtained by flow cytometry. The platform for infection in cell culture was successfully obtained during the studied period, which represents the possibility to apply this model in a continuous practice, to support several biotechnological purposes.","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127996147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot integrated qualification program to improve medication use in a long-term care facility: a hybrid effectiveness-implementation study","authors":"Felipe Augusto dos Santos Oliveira Lima, M. Nascimento, C. P. Rezende, Marina Dias dos Santos, Luciana Raid Farnese, Samilla Dornelas Faria, Aline Silva de Assis Santos, Sabrina Gonçalves Ferreira, Gabriela Oliveira Buzelin Doria, Djenane Ramalho de Oliveira","doi":"10.4322/2179-443x.0772","DOIUrl":"https://doi.org/10.4322/2179-443x.0772","url":null,"abstract":"","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123778563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Journal of Basic and Applied Pharmaceutical Sciences\": five decades of Brazilian scientific publication and contribution to the Pharmacy Area","authors":"A. D. S. Cunha","doi":"10.4322/2179-443x.0600","DOIUrl":"https://doi.org/10.4322/2179-443x.0600","url":null,"abstract":"","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123151577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cell biomarkers as in vitrophotoprotective assays for sunscreen formulations","authors":"S. Figueiredo, F. M. P. Vilela, Thaís Nunes dos Anjos, Amanda Natalina Faria de Pádua, M. Fonseca","doi":"10.4322/2179-443X.0713","DOIUrl":"https://doi.org/10.4322/2179-443X.0713","url":null,"abstract":"Objectives: The aim of this study was to evaluate cellular indicators, which change with exposure to ultraviolet (UV) radiation and can be used as parameters for measuring sunscreens efficiency. Methods: Commercial strains of L929 and HaCaT cells (skin dermis and epidermis, respectively), from the cell bank of Rio de Janeiro, were exposed to different doses of UVA (350 nm) and UVB (309 nm) radiation. The evaluation of the photoprotective potential of sunscreens was analyzed with cell viability, lipid peroxidation and ROS generation tests. Samples of sunscreen with SPF values ranging from 15 to 60 were applied to a quartz plate superimposed on the top of a microplate containing the cell culture, and then the system was irradiated. Results: The viability and lipid peroxidation of the two cell lines remained unchanged after exposure to UVA radiation. When exposed to UVB radiation, the reduction in viability and the increase in lipid peroxides were dose-dependent, that is, they varied from 3.15% to 95.4%, and from 1.2 to 42.7 nM MDA/pg protein, respectively, both for the L929 strain. The dose of 0.5 J/cm2 reduced by 41.4%±1.67 the number of viable cells, and the dose of 30 J/cm2 promoted the oxidation of 42.7 nM of MDA/pg protein. These doses were selected to evaluate the photoprotective effectiveness of commercial sunscreens. Sunscreens exposed to UVB rays could prevent the loss of cell viability (viability remained around 100% for higher SPF) and the formation of lipid peroxides (30 to 80% reduction of peroxide levels). None of the two cell strains, submitted to UVB radiation, formed amounts of intracellular ROS in a dosedependent manner. Under exposure to UVA radiation, only the HaCaT cell line produced the largest amounts of ROS in a dose-dependent manner. After treating these cells with photoprotective formulations (20 J/cm2), the researchers observed a reduction in the amount of ROS formed. Conclusions: The parameters of cell viability and lipid peroxidation were promising to evaluate the photoprotective capacity of sunscreens against UVB radiation. The generation of ROS expressed in the HaCaT strain can discriminate the photoprotective potential of formulations against UVA radiation, as sunscreens reduced the formation of ROS. These results suggest that in vitro tests that evaluate the damage caused to cells can predict cellular indicators of the photoprotective effectiveness of sunscreens and contribute to minimize these tests in the initial phase of product research and development.","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120950544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of immunotherapies for the treatment of high-grade gliomas: a systematic review and meta-analysis","authors":"Edilaine Tiburcio da Silva, H. H. Borba, F. F. Mainka, Isabela Pina Meza, A. Wiens, R. Pontarolo","doi":"10.4322/2179-443x.0768e768","DOIUrl":"https://doi.org/10.4322/2179-443x.0768e768","url":null,"abstract":"","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129992991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tamarind seed polysaccharide (TSP) uses in ophthalmic drug delivery","authors":"Matheus Augusto de Castro, Wallace Mateus Prata, A. Silva-Cunha","doi":"10.4322/2179-443x.0757","DOIUrl":"https://doi.org/10.4322/2179-443x.0757","url":null,"abstract":"author: matheuscastro123@hotmail.com Abstract At present, ophthalmic drug delivery remains a major challenge, given the eye's protective structure and susceptibility to irritation, resulting in poor patient adherence. In order to overcome these constraints, new formulations are continually being developed. The inclusion of Galactoxyloglucan (Tamarind seed polysaccharide (TSP) in such formulations, a natural substance extracted from the seeds of Tamarindus indica , has shown great potential due to its physicochemical properties, high biocompatibility and safety profile. Such properties, have led to its use in formulations for the treatment of dry eye disease, glaucoma, and bacterial keratitis, as well as in dilating eye drops used in eye examinations. In this article, we highlight the most recent TSP-based ophthalmologic formulations, which indicate that this polymer is a strong candidate to reduce adverse effects, improve patient tolerability and rufloxacin administration resulted in slightly higher intraocular drug levels than rufloxacin alone, as shown by the values observed in uninfected (4.62 g/ml; P 0.01), P. aeruginosa -infected (5.63 g/ml; P 0.01), and S. aureus-infected (6.91 g/ml; P 0.001) eyes. In the cornea, TSP-rufloxacin was slightly more effective than rufloxacin alone in regulating P. aeruginosa bacterial proliferation. With regard to S. aureus, TSP-rufloxacin administration resulted in a significant drop in corneal bacteria relative to both rufloxacin-treated and ofloxacin-treated eyes (P 0.05). A further study was conducted evaluating different formulations of rufloxacin (RUF) with TSP, namely two pH 7.2 suspensions of non-salified rufloxacin base, one of which was thickened with TSP; two pH 7.2 solutions of RUF obtained using hydroxypropyl- β cyclodextrin (CD), one with TSP; and two pH 5.0 solutions of rufloxacin hydrochloride (RUF-HCl), one containing TSP 33 . In this study, the suspension containing TSP showed twice the concentration in aqueous humor compared to S-RUF (187.88 vs 67.63 min µg/mL), given the viscosity potential of the polysaccharide. Additionally, pH levels were also shown to influence drug concentration, since the concentration was higher at pH 7.2 when compared to the same formulation at pH 5.0. Rufloxacin solubilization in HP- β -CD (pH 7.2) irrespective of whether or not it contained TSP, showed, poor performance in the final concentration. This could be explained by the fact that the drug interacted strongly with the cyclodextrins and that","PeriodicalId":394666,"journal":{"name":"Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130295840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}