International Journal on Obstetrics and Gynecology最新文献_第2页

Fetal RhD Genotyping using Cell Free Fetal DNA from Maternal Plasma in RhD Negative Women in Pakistan 巴基斯坦RhD阴性妇女血浆中游离细胞胎儿DNA的胎儿RhD基因分型研究

International Journal on Obstetrics and Gynecology Pub Date : 2021-09-18 DOI: 10.51626/ijog.2021.01.00003

{"title":"Fetal RhD Genotyping using Cell Free Fetal DNA from Maternal Plasma in RhD Negative Women in Pakistan","authors":"","doi":"10.51626/ijog.2021.01.00003","DOIUrl":"https://doi.org/10.51626/ijog.2021.01.00003","url":null,"abstract":"Background: The Rhesus (Rh) blood group system is one of the most hemogenic and polymorphic system in the body. RhD negative pregnant women carrying RhD positive fetus are always at the risk of hemolytic disease of the fetus and newborn. Prenatal fetal RhD genotyping leads to appropriate management and targeted anti-D immunoprophylaxis in RhD-positive pregnancies only.\u0000Objective: To determine the accuracy and diagnostic feasibility of non-invasive fetal RhD genotyping by analysis of cell free fetal DNA based on real time polymerase chain reaction (RT-PCR).\u0000Materials and Methods: A total of 100 EDTA-blood samples from pregnant women between 10-32 weeks of gestation were collected. The cell free fetal DNA was extracted from maternal plasma followed by PCR analysis for SRY, RhD (exons 5,7,10) and RASSF1A genes using specific primers. Fetus was considered as RhD-positive when amplification of all targeted RhD exons were observed and in case, where no amplification for RhD exons was detected but Beta-globin gene and SRY gene was amplified, fetus were characterized as RhD-negative. When one or two RhD exons were amplified, the results were predicted to be inconclusive and RT-PCR assay with new\u0000extracted DNA samples was repeated.\u0000Results: Out of 100 pregnancies, none of the sample was found to be inconclusive for fetal RhD status. No false- positive and falsenegative results were registered. The sensitivity, specificity and diagnostic concordance was predicted to be 100%.\u0000Conclusion: The protocol we applied was highly accurate for non-invasive fetal RhD determination. We therefore recommend implementation of non-invasive RhD genotyping in prenatal screening.\u0000Keywords: Cff DNA; Fetal RhD genotyping; Maternal plasma","PeriodicalId":393221,"journal":{"name":"International Journal on Obstetrics and Gynecology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131320081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cost Analysis of Bilateral Salpingectomy Pathology forWomen of Average Risk of Ovarian Cancer 平均卵巢癌风险妇女双侧输卵管切除术病理成本分析

International Journal on Obstetrics and Gynecology Pub Date : 2021-09-18 DOI: 10.51626/ijog.2021.01.00004

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Identifying Maternal Depression in the Perinatal Period inLow Socioeconomic Patients 低社会经济水平患者围产期母亲抑郁的识别

International Journal on Obstetrics and Gynecology Pub Date : 2021-07-22 DOI: 10.51626/ijog.2021.01.00002

引用次数: 0

Colon Cancer: Dietary Risk Factors and the Relevance ofGut Microbiota 结肠癌:饮食风险因素和肠道菌群的相关性

International Journal on Obstetrics and Gynecology Pub Date : 2021-07-19 DOI: 10.51626/ijgld.2021.01.00002

引用次数: 0

Analyzing and Improving Endoscopy Unit Efficiency in anAcademic Tertiary Care Facility 某学术三级医疗机构内窥镜检查单元效率分析与改进

International Journal on Obstetrics and Gynecology Pub Date : 2021-06-11 DOI: 10.51626/ijgld.2021.01.00001

{"title":"Analyzing and Improving Endoscopy Unit Efficiency in an\u0000Academic Tertiary Care Facility","authors":"","doi":"10.51626/ijgld.2021.01.00001","DOIUrl":"https://doi.org/10.51626/ijgld.2021.01.00001","url":null,"abstract":"Background and Aims: Demand for endoscopy continues to increase, and it is critical to identify factors limiting practice efficiency.\u0000The aims of our study were twofold: to identify major bottlenecks in our workflow, and to quantify differences between scheduled procedure times (SPT) and actual procedure times (APT) at our high-volume academic tertiary care endoscopy center.\u0000Methods: We categorized and quantified reasons for delay to the start of the first case of the day through our electronic medical record. To compare SPT and APT, we collected data on all endoscopies from May 2019 to February 2020 and determined mean time\u0000discrepancies of our ten most frequently performed procedures.\u0000Results: The mean preoperative time was 67 minutes. A median of 27 minutes were spent with nursing for preoperative documentation. 74% of first cases started late, and approximately 10% of inpatient cases rolled over to the next day. The most common factors were patient and gastroenterologist tardiness, causing mean delays of 35 and 22 minutes respectively. 48% of cases went over their SPT. Inpatient esophagogastroduodenoscopy (EGD), colonoscopy, and endoscopic retrograde cholangiopancreatography (ERCP) went a mean of 21.3 (p<0.01), 19.4 (p<0.01), and 15.8 (p<0.01) minutes over their SPT, respectively. Outpatient colonoscopy and ERCP went a\u0000mean of 5.23 (p<0.01) and 19.7 (p<0.01) minutes over their SPT, respectively.\u0000Conclusion: Potential targets to improve inefficiency include patient and GI physician tardiness and reducing nursing preoperative documentation time. Inpatient procedures including EGD, colonoscopy, and ERCP along with outpatient colonoscopy and ERCP were significantly delayed and would benefit from additional scheduled time.\u0000Keywords: Endoscopy; Quality improvement; Efficiency","PeriodicalId":393221,"journal":{"name":"International Journal on Obstetrics and Gynecology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132079026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Screening for Small for Gestational Age Fetus: Are weSucceeding? an Audit of Performance at a RegionalHospital 筛选胎龄小的胎儿:我们成功了吗?一家地区医院的绩效审计

International Journal on Obstetrics and Gynecology Pub Date : 2021-04-12 DOI: 10.51626/ijog.2021.01.00001

{"title":"Screening for Small for Gestational Age Fetus: Are we\u0000Succeeding? an Audit of Performance at a Regional\u0000Hospital","authors":"","doi":"10.51626/ijog.2021.01.00001","DOIUrl":"https://doi.org/10.51626/ijog.2021.01.00001","url":null,"abstract":"Background: Fetal growth restriction affects perinatal morbidity and mortality and in practice. symphysis fundal height measurement only identifies 17.3-24.8% of Small for Gestational Age (SGA) fetuses. Plotting on a growth chart and observation of trends has been reported to increase detection rate. Failure to diagnose such fetuses is a recurrent issue at the hospital.\u0000Aims: To determine rate of detection of SGA fetuses during the antenatal period and to determine effect of maternal factors on the detection rate.\u0000Materials and Methods: This was a retrospective chart review of all babies born at Ipswich General Hospital from 2016 to 2018 in singleton pregnancies with birthweight below the tenth percentile and born ≥28 weeks gestation confirmed by first trimester dating ultrasound.\u0000Results: Over 3 years, 760 singleton SGA infants were born. 86.4% had at least 3 antenatal visits after 20 weeks gestation. Although at some stage in pregnancy 52.5% had a symphysis fundal height (SFH) measurement ≥3cm less than gestational age, in only 273 (36%) this resulted in a request for an ultrasound scan to confirm SGA fetus. This happened 53.1% of women who had a scan. Statistical analysis showed the only factor to affect SFH measurement and ultrasound diagnosis of SGA was maternal BMI while maternal age, parity and smoking status had no significant effect.\u0000Conclusion: The rate of detection of SGA is low but may be improved with the introduction of a new program of education and plotting\u0000of SFH on charts to assess trend.\u0000Keywords: Small for gestational age; Symphysis-Fundal height; Antenatal care; Ultrasound","PeriodicalId":393221,"journal":{"name":"International Journal on Obstetrics and Gynecology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127832876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0