Journal of Environmental Microbiology and Toxicology最新文献_第9页

Elevated Molybdenum Concentrations in Soils Contaminated with Spent Oil Lubricant 废润滑油污染土壤中钼含量升高

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.407

H. Yakasai, M. F. Rahman, N. A. Yasid, S. Ahmad, M. Halmi, M. Shukor

引用次数: 7

Assay for Heavy Metals Using an Inhibitive Assay Based on the Acetylcholinesterase from Puntius schwanenfeldii 基于雪旺氏凤蝶乙酰胆碱酯酶抑制法测定重金属

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.412

M. Abdulrasheed, S. Ahmad

{"title":"Assay for Heavy Metals Using an Inhibitive Assay Based on the Acetylcholinesterase from Puntius schwanenfeldii","authors":"M. Abdulrasheed, S. Ahmad","doi":"10.54987/jemat.v5i2.412","DOIUrl":"https://doi.org/10.54987/jemat.v5i2.412","url":null,"abstract":"Acetylcholinesterase (AChE) is frequently used as an inhibitive assay for insecticides. Relatively little is known about AChE inhibition properties on heavy metals. This present study assesses the potential of the AChE from the brain of Puntius schwanenfeldii, which forms as a waste from the aquaculture industry, as an inhibitive assay for heavy metals. The results of the study revealed that Ag+, Cu2+, and Hg2+ completely inhibited AChE activity during initial screening. Furthermore, when tested at various concentrations, the heavy metals demonstrated exponential decay type inhibition curves. The calculated IC50 for the heavy metals Ag+, Cu2+, and Hg2+ were observed to be 0.2498, 0.2427 and 0.2255 mg/L, respectively. The present assay for copper was comparable in sensitivity to assay methods such as immobilized urease, 15-min MicrotoxTM,48-h Daphnia magna, 96-h Rainbow trout, papain and bromelain assays while the present assay for mercury was significantly more sensitive than immobilized urease, equivalent in sensitivity to the rest of the assays, but less sensitive than the papain and bromelain assays. The present assay for silver was more sensitive to all of the assays with the exception of the rainbow trout assay. In conclusion, the findings in this study indicate that the assay, which can be carried out in less than 30 minutes at ambient temperature can be a useful assay for monitoring both insecticides and heavy metals pollution.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"293 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116210992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Sodium Dodecyl Sulfate: A Mini review 十二烷基硫酸钠的生物降解研究进展

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.409

Farah Hanani Muhamad, S. Ahmad, N. A. Yasid

引用次数: 2

Reduction of Molybdenum by Pseudomonas aeruginosa strain KIK-11 Isolated from a Metal-contaminated Soil with Ability to Grow on Diesel and Sodium Dodecyl Sulphate 铜绿假单胞菌KIK-11在柴油和十二烷基硫酸钠污染土壤中对钼的还原作用

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.411

O. Mohamad, H. Yakasai, K. I. Karamba, M. Halmi, M. F. Rahman, M. Shukor

{"title":"Reduction of Molybdenum by Pseudomonas aeruginosa strain KIK-11 Isolated from a Metal-contaminated Soil with Ability to Grow on Diesel and Sodium Dodecyl Sulphate","authors":"O. Mohamad, H. Yakasai, K. I. Karamba, M. Halmi, M. F. Rahman, M. Shukor","doi":"10.54987/jemat.v5i2.411","DOIUrl":"https://doi.org/10.54987/jemat.v5i2.411","url":null,"abstract":"Recently, molybdenum is considered as an emerging pollutant for its extreme toxicity to spermatogenesis in some organisms. Bacterial molybdate reduction to colloidal molybdenum blue (Mo-blue) forms the basis for its bioremediation. Molybdenum-reducing Pseudomonas aeruginosa strain KIK-11 was screened for its potential to degrade hydrocarbons and detergents. Optimal molybdate reduction to Mo-blue in this strain was supported by pH between 5.8 and 6.0, temperatures between 25 and 34 oC, molybdate concentration between 30 and 40 mM and a critical phosphate concentration of between 5.0 and 7.5 mM. The isolate was able to survive and grow on SDS and diesel. However, these compounds did not support Mo-blue production. The best electron donor source facilitating molybdate reduction is glucose, followed by galactose, fructose and citrate respectively. The process was inhibited by heavy metals such as copper (II), mercury (II) and silver (I). The bacterium was able to grow and detoxify multiple toxicants, a novel feat that is important in bioremediation.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124672045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Detection of Pyricularia oryzae Based on Indirect ELISA Using Germinating Conidia as the Antigen Preparation 萌发分生孢子为抗原制备的间接ELISA检测稻瘟霉

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.408

N. A. Masdor, R. Rani, Aseha Yahya, Norhafniza Awaluddin, F. Salam, A. Jack

{"title":"Detection of Pyricularia oryzae Based on Indirect ELISA Using Germinating Conidia as the Antigen Preparation","authors":"N. A. Masdor, R. Rani, Aseha Yahya, Norhafniza Awaluddin, F. Salam, A. Jack","doi":"10.54987/jemat.v5i2.408","DOIUrl":"https://doi.org/10.54987/jemat.v5i2.408","url":null,"abstract":"One of the most destructive fungal diseases in rice crop in Malaysia is the Rice blast disease or Penyakit Karah, which is caused by Pyricularia oryzae Cav. (previously known as Magnoporthe grisea). The disease can cause a loss of yield of more than 50 percent. In this study a sensitive indirect ELISA method was developed for the detection of Pyricularia oryzae through an indirect ELISA method using rabbit polyclonal antibody against different concentrations of Pyricularia oryzae conidia to give seven calibration standard points ranging from 1Ã—101 to 1Ã—109 conidia/mL. The calibration curve shows a typical sigmoidal curve and the fitting of the curve was based on the four-parameter logistic equation. The calculated LOD value was 21.60 conidia/mL (95% confidence interval, 11.63 to 31.57 conidia/mL) with a good correlation coefficient value of 0.999 indicating the model fit the experimental data in an excellent manner. The sensitivity was comparable to another published work which claimed to be the most sensitive to date. This indicate that the current developed method can be utilized in the future as a sensitive method for the rapid and simple detection of this pathogen.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121838743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial Purification of Cholinesterase from Pangasius pangasius using Affinity Chromatography 亲和层析法纯化巴沙鱼胆碱酯酶

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-12-31 DOI: 10.54987/jemat.v5i2.410

L. G. Tham, M. Shukor, M. Syed, N. A. Shamaan, A. Othman

引用次数: 0

Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1 模拟铜对南极假单胞菌DRY1菌株钼还原速率的影响

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-07-31 DOI: 10.54987/jemat.v5i1.417

M. F. Rahman, S. Ahmad, W. MacCormack, L. Ruberto, M. Shukor

{"title":"Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1","authors":"M. F. Rahman, S. Ahmad, W. MacCormack, L. Ruberto, M. Shukor","doi":"10.54987/jemat.v5i1.417","DOIUrl":"https://doi.org/10.54987/jemat.v5i1.417","url":null,"abstract":"Molybdenum reduction by the Antarctic bacterium Pseudomonas sp. strain DRY1 is strongly inhibited by copper. Mo reduction by this bacterium at 10 mM sodium molybdate shows a sigmoidal pattern with lag periods ranging from 7 to 10 h at various concentrations of copper. As the concentration of copper was increased, the overall Mo reduction rate was inhibited with 1.2 mg/L causing the cessation of Mo reduction rate. The modified Gompertz model was utilized to obtain Mo reduction rates at different concentrations of copper. The Mo reduction rates obtained from the modified Gompertz model was then modelled according to the modified Han-Levenspiel, Wang, Liu, modified Andrews and the Amor models. Out of the five models, only Wang, modified Han-Levenspiel and the Liu models were able to fit the curve, whilst the modified Andrews and Amor models were unable to fit the curves. Both the Wang and modified Han-Levenspiel models show acceptable fitting while the Liu model shows poor fitting. Results of the statistical analysis showed that the modified Han-Levenspiel model was the best model based on the lowest values for RMSE and AICc, highest adjusted correlation coefficient (adR2) and values of AF and BF closest to unity. The parameters obtained from the modified Han Levenspiel model, which were Ccrit, ïmax and m which represent critical heavy metal ion concentration (mg/l), maximum reduction rate (nmole Mo blue/h) and empirical constant values were 0.225 (95%, confidence interval from 0.198 to 0.251), 1.200 (95%, confidence interval from 1.180 to 1.220) and 0.443 (95%, confidence interval from 0.261 to 0.626). The modified Han-Levenspiel accurately predicted the critical copper concentration that completely inhibited molybdenum reduction rate in this bacterium.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132322020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Comparison between the modified Gompertz and Churchill death models in modelling the growth of cell culture from leaf-derived cell suspension of Barringtonia racemose 改良的Gompertz和Churchill死亡模型在模拟总状花叶源细胞悬浮液细胞培养物生长中的比较

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-07-31 DOI: 10.54987/jemat.v5i1.418

S. Hussein, M. Shukor

{"title":"Comparison between the modified Gompertz and Churchill death models in modelling the growth of cell culture from leaf-derived cell suspension of Barringtonia racemose","authors":"S. Hussein, M. Shukor","doi":"10.54987/jemat.v5i1.418","DOIUrl":"https://doi.org/10.54987/jemat.v5i1.418","url":null,"abstract":"The growth of plant cell suspension culture often is observed to show a declining phase. Commonly used growth models such as can be used to model sigmoidal growth curves but do not fit curves showing a declining or death phase. In this study, the growth curves of Barringtonia racemose cell suspension under light and dark conditions were modelled according to the Churchill model, which incorporates growth and decline phases, and was compared to the popular modified Gompertz model. For both growth conditions, the Churchill model gave better results for the error functions Root Mean Square Error, the coefficient of determination, adjusted coefficient of determination, bias factor and accuracy factor with the exception of the corrected Akaike Information Criterion (AICc). The regressed parameters or constants obtained from the Churchill model shows growth and decline rates with higher values for the growth rate compared to the decline rates for both growth conditions. The decline and growth rate parameters signify as ï¬1 and ï¬2 for growth under light conditions were 0.367 (95% confidence; 0.103 to 0.632) and 0.796 (95% confidence; 0.458 to 1.134), respectively, while the decline and growth rate parameters ï¬1 and ï¬2 for growth under dark conditions were 0.158 (95% confidence; 0.314 to 0.629) and 1.491 (95% confidence; -0.809 to 3.720), respectively. The 95% confidence interval values were overlapped for both growth rate parameters under light, and dark conditions are indicating that the differences observed were not significant.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127184014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remodelling of the calibration curve for the potentiometric sensing of lamotrigine based on molecularly imprinted polymers using the four-parameter logistic model 基于分子印迹聚合物的拉莫三嗪电位传感标定曲线的四参数logistic模型重构

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-07-31 DOI: 10.54987/jemat.v5i1.413

M. Shukor

{"title":"Remodelling of the calibration curve for the potentiometric sensing of lamotrigine based on molecularly imprinted polymers using the four-parameter logistic model","authors":"M. Shukor","doi":"10.54987/jemat.v5i1.413","DOIUrl":"https://doi.org/10.54987/jemat.v5i1.413","url":null,"abstract":"A standard or a calibration curve allows the determination of an analyte concentration from an experiment. The calibration curve is constructed using a known concentration of analyte. The unidentified concentration of an analyte will then be determined utilizing this standard curve. Similar to immunoassay, ligand-binding interaction in Molecularly Imprinted Polymers (MIPs) also mimics immunoassay in that the calibration curve is sigmoidal in many cases. In a previous study an ion selective electrode based on molecularly imprinted polymers calibration curve for the detection of lamotrigine (LTG) showed a sigmoidal calibration curve but was not modelled according to a sigmoidal dose response model. Using the four-parameter logistic (4PL) equation, the calibration curve was remodelled according to this equation resulting in the calculated values of 19.87, -87.43, -4.614 and 0.6354 for the parameters a and d (maximum and minimum responses), Log EC50 (value that produces a 50% signal response) and Hillslope (slope-like parameter or Hill coefficient). The use of the 4PL model gave a correlation coefficient value of 0.9985 indicating the model excellently fit the experimental data","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126712669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Determination of the detection limit using the four-parameter logistic model for the double-antibody sandwich ELISA for the rapid detection of Bacillus cereus in food 双抗体夹心ELISA快速检测食品中蜡样芽孢杆菌的四参数logistic模型确定检出限

Journal of Environmental Microbiology and Toxicology Pub Date : 2017-07-31 DOI: 10.54987/jemat.v5i1.415

N. A. Masdor

引用次数: 9