Journal of Biorheology最新文献_第6页

Dynamics of amyloid-like aggregation and gel formation of hen egg-white lysozyme in highly concentrated ethanol solution 蛋清溶菌酶在高浓度乙醇溶液中淀粉样聚集和凝胶形成的动力学研究

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.21

I. Dueramae, Shingo Fukuzawa, N. Shinyashiki, S. Yagihara, R. Kita

{"title":"Dynamics of amyloid-like aggregation and gel formation of hen egg-white lysozyme in highly concentrated ethanol solution","authors":"I. Dueramae, Shingo Fukuzawa, N. Shinyashiki, S. Yagihara, R. Kita","doi":"10.17106/JBR.31.21","DOIUrl":"https://doi.org/10.17106/JBR.31.21","url":null,"abstract":"We investigated the mechanisms of amyloidlike aggregation and gel formation in hen egg-white lysozyme (HEWL) in a mixed solvent comprising 90% v/v ethanol in water using dynamic light scattering (DLS) and circular dichroism CD. The mechanism of HEWL in ethanol aqueous solution is interpreted into three stages as: (I) denaturation of HEWL; (II) elongation of amyloid fibrils composed of β-sheet-rich HEWL by lateral aggregation; and (III) gel formation due to the creation of junctions in amyloid fibrils. The transformation of sol to gel can be confirmed by: (1) the oscillation behavior and the rapid increase in the intensity of scattered light; (2) the power-law behavior of the correlation function of scattered light g(2)(t); (3) the appearance of a long-time tail in the distribution function of the decay time G(τ); and (4) the beginning of the reduction in initial amplitude in g(2)(t). The gelation rate was strongly dependent on the protein concentration. The estimated rod length of the amyloid fibrils increased significantly over time. Scanning electron microscopy (SEM) performed on the formation of fibrils in the HEWL gels revealed that the structure was highly heterogeneous, with areas characterized by dense fiber networks interspersed with loose network areas.","PeriodicalId":39272,"journal":{"name":"Journal of Biorheology","volume":"31 1","pages":"21-28"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17106/JBR.31.21","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68196423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Preparation of gelatin scaffold and fibroblast cell culture 明胶支架的制备及成纤维细胞的培养

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.2

Y. Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Y. Maki, Takao Yamamoto, Masumi Uehara, T. Dobashi

引用次数: 1

Effects of urea and NaCl on the fibrinogen cryogelation 尿素和NaCl对纤维蛋白原冷冻的影响

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.12

Y. Toyama, M. Shimizu, M. Ochiai, T. Dobashi

{"title":"Effects of urea and NaCl on the fibrinogen cryogelation","authors":"Y. Toyama, M. Shimizu, M. Ochiai, T. Dobashi","doi":"10.17106/JBR.31.12","DOIUrl":"https://doi.org/10.17106/JBR.31.12","url":null,"abstract":"Fibrinogen is converted to fibrin monomer and forms a three-dimensional gel network in a step wise manner by the enzymatic action of thrombin (thrombin-induced fibrin gelation). On the other hand, when a fibrinogen solution is incubated at low temperatures (e.g. below 4°C), it is converted to a gel without thrombin. The fibrinogen gel induced by lowering temperature is called “cryogel” and is closely related to some diseases. However, the mechanism of fibrinogen cryogelation has not been understood yet. In this study, the effects of urea and NaCl on fibrinogen cryogelation and thrombin-induced fibrin gelation have been studied turbidimetrically. The addition of urea retarded both gelation, and the extent of retardation increased with increasing the concentration of urea. The effects of urea on fibrinogen cryogelation were much larger than those on thrombin-induced fibrin gelation, and fibrinogen cryogelation was completely inhibited by the addition of 150 mM urea. The addition of NaCl retarded fibrinogen cryogelation with increasing the concentration of NaCl. By contrast, the effects of NaCl on thrombin-induced fibrin gelation were only limited. Based on the experimental results, the contributions of hydrogen and ionic bonds to each gelation are discussed.","PeriodicalId":39272,"journal":{"name":"Journal of Biorheology","volume":"31 1","pages":"12-15"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68196298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A method and preliminary results of in silico computer simulation for the formation of mix thrombi with platelet and fibrin 一种血小板与纤维蛋白混合形成血栓的计算机模拟方法及初步结果

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.30

Shinichi Goto, N. Tamura, K. Ayabe, E. Kato, Terumitsu Hasebe, S. Takagi, Yota Kawamura, Shinya Goto

引用次数: 3

Transitions in the in-vivo visualisation of renal physiology 体内肾脏生理可视化的转变

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.40

H. Nakamoto

引用次数: 0

Rheological properties in solutions of wormlike micelle composed of lysophosphatidylcholine/phosphatidylcholine mixture 溶血磷脂酰胆碱/磷脂酰胆碱混合物组成的虫状胶束溶液的流变性能

Journal of Biorheology Pub Date : 2017-01-01 DOI: 10.17106/JBR.31.44

Paphawee Nantarajit, Natdanai Fafaungwithayakul, Natsuda Vichakacharu, S. Matsukawa