Y. Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Y. Maki, Takao Yamamoto, Masumi Uehara, T. Dobashi
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引用次数: 1
摘要
对明胶凝胶片进行紫外照射，提高凝胶的熔化温度Tm。三秒Tm随辐照时间的增加而增加。将Tm大于培养温度37℃的薄片作为细胞培养支架。人成纤维细胞WI-38无论在多大的紫外线照射下都能粘附在薄片上，并深入到薄片上，而癌细胞WI-38- va -13在高紫外线照射下形成球状体，并不能单独粘附在薄片上。该支架的细胞选择性可用于生物学和医学研究。
Preparation of gelatin scaffold and fibroblast cell culture
A gelatin gel sheet was UV-irradiated to raise the gel melting temperature Tm. Three second of Tm increased proportionally to irradiation time tUV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of tUV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.
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