{"title":"Rooting and acclimatization of in vitro propagated microshoots of the Ericaceae","authors":"A. Erst, A. Gorbunov, A. Karakulov","doi":"10.37855/jah.2018.v20i03.31","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.31","url":null,"abstract":"The effective methods of rooting and acclimatization in the sphagnum moss of in vitro propagated microshoots of commercially promising plants: Rhododendron ledebourii, R. hybridum cv. ‘Cunningham’s White’ and Vaccinium uliginosum cv. ‘Golubaya rossyp’ were developed for the first time. Two methods were studied: 1) rooting in vitro and acclimatization in the substrate and 2) rooting and acclimatization ex vitro in the substrate. Taking into account two factors (rooting of microshoots only in vitro conditions and only from already rooted in vitro regenerants), we have achieved 100 % of rooting of bog blueberry cv. ‘Golubaya rossyp’ and 73 % of plants adapted to ex vitro conditions. The method of ex vitro rooting in the substrate has been proved as effective for R. ledebourii, rooting of it’s shoots reached 80-90 % with 87 % viable plants. R. hybridum cv. ‘Cunningham’s White’ showed the greatest plasticity among the studied species and cultivars on ability to rooting of microshoots. All tested methods gave up to 90-100 % rooted and adapted plants of this cultivar. It was revealed that in vitro rooting microshoots, followed by acclimatization in the substrate was optimal for V. uliginosum cv. ‘Golubaya rossyp’; for R. ledebourii – the rooting and acclimatization in the substrate; and for R. hybridum cv. ‘Cunningham’s White’ both methods were effective. From a commercial point of view, the proposed methods decrease the costs of plant production significantly through a reduction in the time and labour needed to obtain well-rooted and acclimatized Ericaceae microplants. Therefore, it could make the micropropagation of commercially promising Ericacea plants in the nursery industry both possible and profitable.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49261964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. A. Animasaun, S. Oyedeji, R. Ingalhalli, R. Krishnamurthy
{"title":"Polymorphism and genetic diversity assessment of some ornamental ferns by microsatellite (ISSR) markers","authors":"D. A. Animasaun, S. Oyedeji, R. Ingalhalli, R. Krishnamurthy","doi":"10.37855/jah.2018.v20i03.41","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.41","url":null,"abstract":"This study assessed the genetic diversity of six ornamental ferns in parks and gardens in Nigeria using inter-simple sequence repeat (ISSR) markers. Genomic DNA were extracted from the samples and Polymerase Chain Reaction (PCR) was performed using seven ISSR markers. The elcetrophoregram showed amplicon size ranged from 100bp-1Kb among the ferns. A total of 87 bands were generated with 71.26 % polymorphism and 28.73 % monomorphic bands. The average number of band per primer was 12.4 and polymorphism range was between 31.23-100 %, the highest polymorphism was obtained with ISSR2. The mean Nei’s genetic diversity was 29 % while the Shannon’s index was 43.5 %. The pair of Adiatum capilus-veneris and Pteris acanthoneura had maximum genetic distance of 0.6592 while A. capilus-veneris and A. furcans are the closest species. At genetic similarity of 78 %, the cluster analysis revealed two major groups. Group-1 comprised of four species, where P. acanthoneura is a distant member, the other three species in the group are closely related. Group-2 on the other hand had two closely related Adiantum species. The study concludes that ISSR markers are effective in the genetic study of the ferns and the genetic diversity information provided could be utilized for selection, improvement and conservation of the ornamental plants.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46508039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mishra, M. Mishra, Shantha Selva Kumari, P. Shirke, A. Srivastava, S. Saxena
{"title":"Studies on anatomical behaviour of PaLCuV infected papaya (Carica papaya L.)","authors":"M. Mishra, M. Mishra, Shantha Selva Kumari, P. Shirke, A. Srivastava, S. Saxena","doi":"10.37855/jah.2018.v20i03.38","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.38","url":null,"abstract":"Papaya leaf curl virus (PaLCuV) of geminiviridae family is a major threat to papaya plants in the world. The major visual characteristics of PaLCuV infected plants are downward and inward rolling and curling of leaves in the form of an inverted cup and thickening of veins. Microscopic observation showed that in the healthy papaya leaf, stomata guard cell size was 19-20 μm. However, it increased significantly in infected plant leaves up to 29-30 μm. This observation suggested that stomatal density and guard cell size were changed due to puckered anatomy of leaf. SEM analysis revealed that subsidiary or accessory cells of guard cells were less turgid and the arrangement of starch grains was disturbed as compared to healthy plant leaves. Light microscopic, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) analyses of symptomatic leaves showed the puckering leaf lamina due to presence of loosened cells in its tissues and hyper-accumulation of Ca2+ ions. High accumulation of Ca2+ in PaLCuV infected leaves as compared to healthy leaves which might be the cause of hypertrophy and thickening of veins of infected papaya leaves. Uniform trichomes/hairs/nodular structures were present in midrib of healthy leaf but were missing in infected leaf. The infected midrib showed scantly distributed floret-like structures instead of a smooth trichomes/hairs/nodular structures in midrib of the healthy leaf.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48033543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of foliar boron applications on yield and quality of Medjool date palm","authors":"Hashem S. Al-hajjaj, Jamal Y. Ayad","doi":"10.37855/jah.2018.v20i03.32","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.32","url":null,"abstract":"Effects of foliar boron (B) application as H3BO3 on yield and fruits quality of date palm (Phoenix dactylifera L. var. Medjool) were investigated. The experiment was conducted on 15 years old trees during 2017 growing season. Five different B levels (0, 400, 800, 1200 and 1600 ppm) were applied in a solution of 20 liters∙tree-1 at three equal doses in May, June and July. Fruit yield and quality parameters were determined at maturity harvest stage (tamer). The results showed that foliar B significantly affected yield, bunch weight, fruit set, fruit physicalcharacters and fruit quality. Boron application at the rate of 1600 ppm resulted in 27 % increase in fruits yield (67.7 vs. 53.4 kg∙tree-1), 16 % increase in fruit fresh weight and flesh weight (22.0 vs. 18.9 g.fruit-1), (20.9 vs. 18.0 g fruit-1), respectively, fruit size (49.97 vs. 45.3 mm) and fruit set (88 % vs. 81 %) compared to control. However, the lower rate of boron (400 ppm) was more effective in enhancing fruit quality indices. Total soluble solid (Brix % 75.0), fruit firmness (5.56 kg∙cm-2), glucose content (43.17 %), fructose content (39.28 %) and fruit antioxidant activity (80 %) were significantly increased as compared to control. On other hand, date syrup pH, fruit density and fruit skin separation were not affected by boron application. Overall, the results presented here recommended the use of foliar rates of boron ranging between 400 to 1600 ppm to enhance fruit yield and quality in Medjool dates.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44515137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aparna Veluru, M. Neema, K. Prakash, A. Arora, P. Naveen Kumar, M. Singh
{"title":"Regulation of chrysanthemum cut flower senescence using 5-sulfosalicylic acid and aluminium sulphate","authors":"Aparna Veluru, M. Neema, K. Prakash, A. Arora, P. Naveen Kumar, M. Singh","doi":"10.37855/jah.2018.v20i03.42","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.42","url":null,"abstract":"Vase-life is a key factor for evaluating the post-harvest quality of cut flowers that determines their marketability. Chrysanthemum is one of the top most cut flowers sold in international flower markets. In the present study, trials were conducted to improve the postharvest life of chrysanthemum cut flowers using preservative solutions such as 5-sulfosalicylic acid (5-SSA) and aluminium sulphate (Al2(SO4)3) alone or in combination with 1.5 % sucrose. Treatments using 5-SSA (100 ppm and 150 ppm) or 200 ppm Al2(SO4)3 along with 1.5 % sucrose showed a significant increase in vase-life, fresh weight of the cut stems, vase solution uptake, membrane stability index of the petals and leaf chlorophyll as compared to other treatments. Among different vase solutions evaluated, T10 (200 ppm Al2(SO4)3 +1.5 % sucrose) gave maximum vase life of22.3 days, followed by T8 (5-SSA 150 ppm + 1.5 % sucrose) and T6 (100 ppm 5-SSA+ 1.5 % sucrose) treatments with 20.85 and 19.85 days respectively as compared to 17.84 days in control. High concentrations of both the chemicals (5-SSA and Al2(SO4)3) without sucrose showed toxicity symptoms.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44584272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of zinc fertilization on growth and leaf nutrient content of Celosia argentea L.","authors":"Olukunle Kolade, D. Harp, C. Jones, José G. López","doi":"10.37855/jah.2018.v20i03.35","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.35","url":null,"abstract":"Zinc is an important micronutrient for both plant and human health, and dietary zinc is insufficient in many areas of the world, caused by a diet dominated by grains and vegetables grown on zinc deficient soils. Celosia argentea L. is an herbaceous annual adapted to difficult soil conditions, low soil nutrient content, and hot weather and is commonly grown as a leafy vegetable in many parts of Africa. This study was conducted to evaluate zinc fertilization of celosia at levels higher than normally recommended, but below toxic levels, to determine the effect on plant performance and dietary value. Celosia was grown in a controlled environment with four zinc fertilizer treatments (0, 50, 100 and 150 mg Zn·kg-1). Zinc fertilization had minor effects on plant growth, with differences only found in plant height and root fresh weight with both decreasing at fertilization levels above 100 mg Zn·kg-1. Higher zinc fertilization increased root nitrogen, leaf and root zinc, potassium and magnesium concentrations, though leaf magnesium decreased at the highest zinc fertilizer levels. At 50 mg Zn·kg-1, zinc fertilization toxicity symptoms were unexpessed thus it can improve plant performance and provide potentially significant dietary benefits to people with limited access to zinc in their diet.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41523038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Understanding the genetic variability, heritability and association pattern for the characters related to reproductive phase of carrots (Daucus carota L.) in tropical region","authors":"D. Manikanta, A. P. Chaitra, S. Cholin","doi":"10.37855/jah.2018.v20i03.39","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.39","url":null,"abstract":"Carrot is a sexully propagating, biennial root crop. Flowering related characters and germination parameters have a significant role on the root quality and productivity in the succeeding generations. Larger genetic variation exists in nature for seed and umbel characters due its out crossing behavior. In order to study, the nature of genetic variability, heritability and the association pattern, 48 open pollinated varieties were evaluated in tropical region of Karnataka, India during 2015 for 27 characters including seed, umbel and germination parameters. Both qualitative and quantitative characters were used for the study. The data was subjected to statistical analysis for genetic variability components such as genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), heritability in broad sense (h2) genetic advance (GA) and Genetic advance as percent mean (GAM) and Pearson’s correlation. Genetic variability and heritability coupled with genetic advance as percent mean (GAM) were high for number of umbellates per umbel, bolting tendency and umbel weight. Many other characters showed low to moderate genetic variability coupled with moderate heritability and GAM. From the correlation coefficient analysis few characters viz., density of flowers in umbels, number of umbellates/ umbel, umbel shape, bolting tendency showed positive association with seed and umbel yield. Seed length and size of spines on the seeds affects the germination % negatively. Since, the present study involves maximum characters and good number of cultivars with wider genetic background; hence, detailed understanding of the genetic variability and association pattern for these characters in the reproductive phase of carrot would provide useful information for breeders as well as seed scientists.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47523944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Micropropagation of Anthurium through suspension culture using in vitro shoots","authors":"Bhavana G.P, B. S. Kumudini, C. Aswath","doi":"10.37855/jah.2018.v20i03.34","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.34","url":null,"abstract":"A simpler and more efficient protocol for mass propagation of Anthurium from somatically derived in vitro shoots through suspension culture was developed. Shoot proliferation was obtained with agar-solidified MS medium as well as liquid MS medium, at various concentration of BAP. After 45 days of incubation at 80 rpm, both the media gave best results at 8.8 μM BAP, whereas higher number (29.333±0.342) of shoots was obtained with liquid medium compared to solid medium (7.667±0.342). The matured plantlets were harvested and subjected to acclimatization after 30 days of primary and 60 days of secondary hardening and then they were transferred to field. Survival rate of plants (71.3 %) in liquid medium was higher compared to the ones from solid medium (43.0 %).","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45107947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Madhumathi, V. Bhargav, D. Srinivasa Reddy, P. Lalitha Kameshwari, D. Sreedhar, T. Naga Lakshmi
{"title":"Assessment of chrysanthemum (Chrysanthemum morifolium Ramat.) germplasm for commercial cultivation under Rayalaseema region of Andhra Pradesh","authors":"C. Madhumathi, V. Bhargav, D. Srinivasa Reddy, P. Lalitha Kameshwari, D. Sreedhar, T. Naga Lakshmi","doi":"10.37855/jah.2018.v20i03.37","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.37","url":null,"abstract":"Fifty nine chrysanthemum (Chrysanthemum morifolium Ramat.) genotypes were evaluated for their performance under southern zone of Andhra Pradesh at HRS Farm, Anantharajupeta, Y.S.R. Kadapa, Andhra Pradesh. Significant differences were observed among the genotypes for all vegetative, flowering and yield traits studied. Genotype Selection-5 (56.50 cm) recorded maximum plant height, and plant spread was maximum in two genotypes Chandini and Violet (51.83 cm). Genotypes Sugandha Yellow and Sugandha White produced maximum (11.67) number of primary branches/plant while least in Yellow Cherub (2.33). Earliest bud appearance was observed in genotype Violet (61.67 days), whereas genotype Meera (113.52 days) was late. The highest number of flowers/spray and spray length was produced by genotype Yellow Star and Selection-2 (41.67 and 44.87 cm), respectively. Flower diameter was maximum in Firtation (10.06 cm), however, number of flowers/plant was highest in HYDC7 (287). The weight of single flower and 100 flower weight was maximum in Mother Teresa (4.96 g and 495.50 g). Keeping in view of these characters, genotypes Mother Teresa, Firtation and Chrysanthemum Yellow were found to be highly suitable to grow under Rayalaseema conditions of Andhra Pradesh for loose flower production. Based on hierarchical cluster of the morphological traits, the genotypes were grouped into three. Results of principal component Analysis (PCA) were also in conformity with the results of cluster analysis. Key word: Chrysanthemum, genotype, morphological traits, cluster analysis and PCA","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44902926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Gerami, P. R. Moghaddam, R. Ghorbani, A. Hassani
{"title":"Influence of planting date and plant density on morphological characteristics, seed yield and essential oil percentage of oregano (Origanum vulgare L.)","authors":"F. Gerami, P. R. Moghaddam, R. Ghorbani, A. Hassani","doi":"10.37855/jah.2018.v20i03.30","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.30","url":null,"abstract":"Over utilization of medicinal plants from wild habitat is causing genetic diversity loss of these valuable plants. In order to determine the optimum planting date and density of oregano for successful production, a field experiment was conducted at the Faculty of Agriculture, Urmia University, Iran. The experimental design was split-plot based on randomized complete blocks with three planting dates (April 19, May 5 and May 20) as main plots and four plant densities (8.33, 10, 12.5 and 16.67 plants m-2) were assigned as sub-plots, all of which replicated three times. Results showed that planting date had significant effects on all traits except the stem proportion and 1000 seed weight. The highest values for all traits were obtained from the first date of planting, whereas values decreased with later planting dates. Also, stem number, plant spread, fresh and dry herb yield, seed yield and essential oil yield significantly influenced by plant density. With increase in plant density, stem number in per plant and plant spread decreased. The maximum fresh and dry herb yield (10618.23 and 2934.36 kg ha-1, respectively) and essential oil yield (42.786 kg ha-1) were obtained at the highest plant density. In conclusion, first planting date with higher plant density caused the greater essential oil yield, fresh and dry herb yield.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43973690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}