Journal of the University of Malaya Medical Centre最新文献

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THE ASSOCIATION BETWEEN PERFECTIONISM AND SOCIAL ANXIETY AMONG ADOLESCENTS IN SELANGOR, MALAYSIA 马来西亚塞兰戈青少年完美主义与社交焦虑的关系
Journal of the University of Malaya Medical Centre Pub Date : 2020-01-21 DOI: 10.22452/JUMMEC.VOL23NO1.1
Chuah Peng Aik, M. Rabbani, Mansour Amini
{"title":"THE ASSOCIATION BETWEEN PERFECTIONISM AND SOCIAL ANXIETY AMONG ADOLESCENTS IN SELANGOR, MALAYSIA","authors":"Chuah Peng Aik, M. Rabbani, Mansour Amini","doi":"10.22452/JUMMEC.VOL23NO1.1","DOIUrl":"https://doi.org/10.22452/JUMMEC.VOL23NO1.1","url":null,"abstract":"Social anxiety is explained as noticeable anxiety or fear in one or more social situations where the individual is exposed to possible analysis by others. This research is aimed to determine the association between perfectionism and social anxiety among adolescents in Selangor, Malaysia. A final sample of 327 participants, between 13 to 18 years old, was collected. Data collection was conducted in two public secondary schools and two private high schools in Selangor. This survey included three instruments of analysis: The Child-Adolescent Perfectionism Scale, Kutcher Generalized Social Anxiety Disorder Scale for Adolescents and Demographic Questionnaire. The results showed that there is a significant positive association between perfectionism and social anxiety. This study has proved the positive association between perfectionism and social anxiety among adolescents in Selangor, Malaysia. Therefore, it is important to identify those students with these issues and guide them in an effective way to overcome the issues.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"23 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42745530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ETHNIC DIFFERENCES IN URINARY INCONTINENCE AMONG WOMEN AGED 55 YEARS AND OVER: RESULTS FROM THE MALAYSIAN ELDERS LONGITUDINAL RESEARCH (MELOR) 55岁及以上女性尿失禁的种族差异:马来西亚老年人纵向研究(MELOR)的结果
Journal of the University of Malaya Medical Centre Pub Date : 2020-01-21 DOI: 10.22452/JUMMEC.VOL23NO1.4
Gayaithiri Ramasandran, K. Yen, Sumaiyah Mat, S. Kamaruzzaman, Chin Ai Vyrn, Ong Teng Aik, A. Razack, T. Pin
{"title":"ETHNIC DIFFERENCES IN URINARY INCONTINENCE AMONG WOMEN AGED 55 YEARS AND OVER: RESULTS FROM THE MALAYSIAN ELDERS LONGITUDINAL RESEARCH (MELOR)","authors":"Gayaithiri Ramasandran, K. Yen, Sumaiyah Mat, S. Kamaruzzaman, Chin Ai Vyrn, Ong Teng Aik, A. Razack, T. Pin","doi":"10.22452/JUMMEC.VOL23NO1.4","DOIUrl":"https://doi.org/10.22452/JUMMEC.VOL23NO1.4","url":null,"abstract":"Background: Urinary incontinence is a common problem among older women. The objective of this study was to determine the prevalence of urinary incontinence among older women aged 55 years and over in the Klang valley, the most densely populated urban area in Malaysia, and to determine any potential ethnic differences.Methods: This study used cross-sectional data from the Malaysian Elders Longitudinal Research (MELoR) study. A total of 863 participants were included, with a mean age of 67.97 ± 7.50 (S.D.). They were selected by simple random sampling from the electoral rolls of three parliamentary constituencies.Results: The prevalence of urinary incontinence was 30.8%. Mixed urinary incontinence (UI) was present in 44.7%, stress UI in 39.1% and urge UI in 16.2%. Ethnic Malays (38%) were more likely than ethnic Indians (32.3%) and Chinese (29.7%) to have UI. Ethnic Malays were also more likely to have stress UI and Indians were more likely to have urge UI .Conclusion: The prevalence of UI in this study was comparable to other Asian and worldwide studies. The significant association between ethnicity and UI, however, has not been reported in any previous studies. Further studies should identify factors which may determine these ethnic differences in UI.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"23 1","pages":"18-22"},"PeriodicalIF":0.0,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47451371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A RARE CASE OF AN ACUTE ON CHRONIC THORACOABDOMINAL AORTIC DISSECTION 一例罕见的急慢性胸腹主动脉夹层
Journal of the University of Malaya Medical Centre Pub Date : 2020-01-01 DOI: 10.22452/JUMMEC.VOL23NO2.15
A. Mubarak, W. N. A. Shukri, A. Ismail
{"title":"A RARE CASE OF AN ACUTE ON CHRONIC THORACOABDOMINAL AORTIC DISSECTION","authors":"A. Mubarak, W. N. A. Shukri, A. Ismail","doi":"10.22452/JUMMEC.VOL23NO2.15","DOIUrl":"https://doi.org/10.22452/JUMMEC.VOL23NO2.15","url":null,"abstract":"Aortic dissection (AD) is a rare cardiovascular emergency with a high rate of mortality. Most patients present with typical signs and symptoms of AD. However, atypical presentation increases risk of delay in diagnosis and treatment. Patients may present with syncope, seizures or other neurological symptoms which may deceive the diagnosis of AD. Dissection can be in the acute or chronic phase, but there has been no documentation of an acute dissection which occurs in a chronic dissection. We hereby to report a case of an acute on chronic thoraco-abdominal dissection.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"23 1","pages":"103-106"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68653031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE CONCEPTUAL FRAMEWORK: ESTABLISHING HEALTH LITERACY MODULE FOR INDIGENOUS PEOPLE IN MALAYSIA 概念框架:为马来西亚土著人民建立卫生知识普及模块
Journal of the University of Malaya Medical Centre Pub Date : 2019-12-23 DOI: 10.22452/jummec.vol22no2.10
Norma Alias, M. Jaafar, A. Yusof, M. Isa
{"title":"THE CONCEPTUAL FRAMEWORK: ESTABLISHING HEALTH LITERACY MODULE FOR INDIGENOUS PEOPLE IN MALAYSIA","authors":"Norma Alias, M. Jaafar, A. Yusof, M. Isa","doi":"10.22452/jummec.vol22no2.10","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.10","url":null,"abstract":"This paper proposed a conceptual model on developing a new Health Literacy Module for Indigenous People in Malaysia. This proposed model could be used as a reference for researchers to develop new strategies to overcome health issues on Indigenous People. The proposed model incorporated three (3) phases which are (i) Phase 1: To determine the Knowledge, Attitude & Practice (KAP) on parasitic infections among Indigenous People, (ii) Phase 2: To develop self-useful kit for Indigenous People according to the needs in Phase 1, (iii) Phase 3: To develop a health literacy module of parasitic infections as a referral for Indigenous People. This research will have a significant contribution to provide an in-depth knowledge on identification of health care problems related to parasitic infections towards the development of health literacy module for Indigenous People. Authors will propose new or adapted or/and adopted tools/products/guidelines to be established based on the findings of this research. The findings are aimed to improve quality of life for Indigenous People in Malaysia.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"22 1","pages":"64-67"},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41370261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PRACTICES, ATTITUDES AND AWARENESS OF SUBURBAN ADOLESCENCE SCHOOL CHILDREN TOWARDS ORAL HYGIENE: SCHOOL- BASED SURVEY 郊区青少年口腔卫生行为、态度和意识的学校调查
Journal of the University of Malaya Medical Centre Pub Date : 2019-12-20 DOI: 10.22452/jummec.vol22no2.9
V. N. Ahmad, Norazuwa Hazman, Alya Syazana Razali, Zutifarihiah Rasdi, Rahayu Zulkapli
{"title":"PRACTICES, ATTITUDES AND AWARENESS OF SUBURBAN ADOLESCENCE SCHOOL CHILDREN TOWARDS ORAL HYGIENE: SCHOOL- BASED SURVEY","authors":"V. N. Ahmad, Norazuwa Hazman, Alya Syazana Razali, Zutifarihiah Rasdi, Rahayu Zulkapli","doi":"10.22452/jummec.vol22no2.9","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.9","url":null,"abstract":"Background: Oral hygiene is importance in maintaining a good oral health as it may affect the general healthof the body. Adolescence features a stage of significant caries activity for countless individuals. Thus, thepurpose of this study was to assess the knowledge, attitude and practice among adolescence suburban schoolchildren towards oral hygiene.Methods: A cross-sectional study in which self-administered questionnaire was distributed to 300 studentsat selected suburban public schools in Selangor based on stratified sampling. The questionnaire comprised ofsocio-demographic characteristics, knowledge about oral hygiene, attitude towards oral hygiene and practicefor prevention of oral disease. Students who had previously resided in urban areas for a minimum period of1 year in the past 5 years were excluded. Data were analyzed by using descriptive analysis and Chi-squaredtest in SPSS version 21.Results: A total of 252 answered questionnaires were returned. The results of the study indicated low level ofawareness for oral hygiene; 75.4 % showed lack of knowledge on dental plaque, 81.3% did not use floss, only46.0 % brush their teeth twice per day and 27.8% visited a dentist last 6 months ago. A good attitude towardsoral hygiene is still lacking, nevertheless a majority of the respondents have slight negative view toward dentalvisit which is mostly influenced by their anxiety.Conclusion: This study revealed deficient of oral hygiene awareness among adolescence suburban schoolchildren in certain aspects of dental hygiene. It therefore highlights that urbanization does not always correlatewith high level of oral hygiene awareness.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"22 1","pages":"56-63"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41971593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ANTHROPOMETRIC CHARACTERISTICS OF MALAYSIAN COMPETITIVE POWERLIFTERS WITH PHYSICAL DISABILITIES 马来西亚残疾运动员的人体测量学特征
Journal of the University of Malaya Medical Centre Pub Date : 2019-12-03 DOI: 10.22452/jummec.vol22no2.8
M. Hamid, S. Ghazali, S. Karim
{"title":"ANTHROPOMETRIC CHARACTERISTICS OF MALAYSIAN COMPETITIVE POWERLIFTERS WITH PHYSICAL DISABILITIES","authors":"M. Hamid, S. Ghazali, S. Karim","doi":"10.22452/jummec.vol22no2.8","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.8","url":null,"abstract":"Studies on the anthropometric, physical and physiological characteristics among Malaysian Paralympic powerlifters are limited. This study examined the sociodemographic, clinical information and anthropometric physical parameters of Paralympic powerlifters in Malaysia. \u0000A cross-sectional study was conducted during a Powerlifting Workshop and National Championship in 2016. A structured questionnaire was used to collect data on athlete’s sociodemographic, sports participation history and medical information. All participants underwent a structured physical medical examination and anthropometric assessments. \u0000Fifty-two powerlifters took part in this study. Mean age of participants was 24.50±SD8.25 year. Majority of participants were men (82.7%) and most had spinal cord injury (34.6%) or amputation of the lower limb (26.9%). Most powerlifters competed at district and state level championships and 42.3% had represented Malaysia at international competitions. Women powerlifters had a significantly higher amount of body fat compared to men (35.61% vs 19.80%; p=0.003). Men power-lifters had significantly longer arm and forearm length (30.10±IQR3.00 cm vs 23.00±IQR2.13 cm; p=0.020).","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":"22 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45025323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
HEALTHCARE PROVIDERS’ KNOWLEDGE TOWARDS MEDICATION USE IN BREASTFEEDING: AN INTERVENTIONAL STUDY 医疗保健提供者对母乳喂养药物使用的认识:一项干预性研究
Journal of the University of Malaya Medical Centre Pub Date : 2019-11-05 DOI: 10.22452/jummec.vol22no2.6
Nurul Izzaty Ramli
{"title":"HEALTHCARE PROVIDERS’ KNOWLEDGE TOWARDS MEDICATION USE IN BREASTFEEDING: AN INTERVENTIONAL STUDY","authors":"Nurul Izzaty Ramli","doi":"10.22452/jummec.vol22no2.6","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.6","url":null,"abstract":"Purpose: Most of postpartum women have to take at least one medication; so safety of medication needs to be considered when prescribing and dispensing the medication to them. In 2017, 11% of 815 questions received by Pharmacy Drug Information Services at seven clinics in Dungun were related to medication use in breastfeeding. Thus, this study was carried out to evaluate attitude of healthcare providers (HCPs) and to investigate the effect of knowledge about medication use in breastfeeding among HCPs; pre and post educational intervention. \u0000 Method: An interventional study was conducted among medical officers, assistant medical officers, pharmacists and pharmacist assistants from seven clinics in district of Dungun, Malaysia. The questionnaires were distributed during pre and post-intervention period. The interventions in this study were continuous medical education (CME) and booklet regarding medication use for breastfeeding women. Data were entered and analyzed using Statistical Package for Social Studies, (SPSS) and presented as frequencies, means, and standard deviations. \u0000Result: A total of 50 HCPs were enrolled in this study and over 20% of them advised mother to discontinue breastfeeding whenever she had to take any medication. The knowledge of HCPs about medication use in breastfeeding women was significantly improved (Z= -5.917, p<0.001) after the educational intervention.  \u0000Conclusion: CME and simplified booklet tend to have positive impact on the knowledge of HCPs regarding medication use in breastfeeding.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44119651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RELATIONSHIP BETWEEN ISOKINETIC LEG STRENGTH AND KNEE FRONTAL PLANE PROJECTION ANGLE DURING SINGLE LEG SQUAT AMONG MALE JUNIOR ATHLETES 男青年运动员单腿深蹲时等速腿部力量与膝关节正面投影角度的关系
Journal of the University of Malaya Medical Centre Pub Date : 2019-11-05 DOI: 10.22452/jummec.vol22no2.7
Mohamad Saufi Zuhan Mail, Norain Mohd Azhar, Nor Fatinah Affandi, S. Shaharudin, S. Agrawal, Lee Ming Chee
{"title":"RELATIONSHIP BETWEEN ISOKINETIC LEG STRENGTH AND KNEE FRONTAL PLANE PROJECTION ANGLE DURING SINGLE LEG SQUAT AMONG MALE JUNIOR ATHLETES","authors":"Mohamad Saufi Zuhan Mail, Norain Mohd Azhar, Nor Fatinah Affandi, S. Shaharudin, S. Agrawal, Lee Ming Chee","doi":"10.22452/jummec.vol22no2.7","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.7","url":null,"abstract":"Background: Those with increased dynamic knee valgus are vulnerable to increased risk of non-contact kneeinjuries. However, studies on the top down kinetic chain of lower limb mechanics during dynamic motionssuch as single leg squat (SLS) among trained males were scarce.Objective: The objective of the study was to evaluate the relationship between isokinetic hip and knee strengthand frontal plane projection angle (FPPA) of the knee joint during SLS.Methods: Thirty-two male junior athletes (twelve cyclists, ten runners and ten squash players) were screenedfor excessive dynamic knee valgus (DKV) prior to participation. Only those within the normal value of DKV wereincluded. Their hip and knee isokinetic strength in sagittal plane were evaluated at 60o/s of angular velocityfor both legs using dynamometer. Two dimensional knee FPPA was evaluated during SLS at 60o of knee flexion.Pearson correlation was evaluated between knee FPPA during SLS and isokinetic leg strength.Results: Correlations between knee FPPA and hip and knee isokinetic strength were not statistically significantexcept between knee flexion peak torque/body weight (r = -0.35, p = 0.05) and hamstring to quadriceps ratio(r = -0.39, p = 0.03) of non-dominant leg.Conclusions: Isokinetic hip and knee strength and knee FPPA during SLS was correlated only for non-dominantleg during SLS among male junior athletes. DKV during SLS may be reduced through strengthening the musclesaround hip and knee joints.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43723987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
INCIDENCE, PATTERNS AND RISK FACTORS OF INJURY & ILLNESS AMONG ATHLETES DURING THE MALAYSIAN HIGHER EDUCATION GAMES (SUKIPT) 2018 2018年马来西亚高等教育运动会期间运动员受伤和疾病的发生率、模式和风险因素
Journal of the University of Malaya Medical Centre Pub Date : 2019-11-05 DOI: 10.22452/jummec.vol22no2.3
R. Muhammad Harith, A. Shariff
{"title":"INCIDENCE, PATTERNS AND RISK FACTORS OF INJURY & ILLNESS AMONG ATHLETES DURING THE MALAYSIAN HIGHER EDUCATION GAMES (SUKIPT) 2018","authors":"R. Muhammad Harith, A. Shariff","doi":"10.22452/jummec.vol22no2.3","DOIUrl":"https://doi.org/10.22452/jummec.vol22no2.3","url":null,"abstract":"Background: Despite regularly participating in international and national level multisport events, there is still limited data on the pattern of injuries and illnesses and factors associated with injuries and illnesses in Malaysia. Such information is crucial to instil preventive measures because sustaining injuries during competition could hamper the athlete’s performance. Methods: This cross-sectional study investigated the incidence, patterns and risk factors of injuries and illnesses among athletes throughout SUKIPT 2018, from the 2nd to the 10th of February 2018. All injuries and illnesses treated by tournament medical personnel were reported using a standardised online injury reporting form. This form was adapted from the injury surveillance form used by the International Olympic Committee. Results: A total of 6071 athletes from 80 contingents took part in SUKIPT 2018. During the nine days of competition 323 injuries and 48 illnesses were reported, resulting in an incidence of 5.3 injuries and 0.8 illness per 100 athletes. Approximately 6% of the athletes sustained at least one injury or illness. Conclusion: In summary, the incidence of injuries and illnesses among athletes during SUKIPT was 5.3 and 0.8 per 100 athletes, respectively. Muscle strain/rupture/tear was the most common pattern of injury while collision with another athlete was the most frequent mechanism of injury. Meanwhile, the respiratory system was the most commonly affected by illness and infection was the most prevalent cause of illness.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45998956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CELL SURFACE PROTEINS OF CRYPTOCOCCUS NEOFORMANS MEDIATE ADHERENCE AND INTERNALISATION INTO MAMMALIAN CELLS 新型隐球菌的细胞表面蛋白介导哺乳动物细胞的粘附和内化
Journal of the University of Malaya Medical Centre Pub Date : 2019-11-01 DOI: 10.22452/JUMMEC.VOL22NO2.4
K. Choo, V. Chin, P. Chong, S. H. Ho, V. Yong
{"title":"CELL SURFACE PROTEINS OF CRYPTOCOCCUS NEOFORMANS MEDIATE ADHERENCE AND INTERNALISATION INTO MAMMALIAN CELLS","authors":"K. Choo, V. Chin, P. Chong, S. H. Ho, V. Yong","doi":"10.22452/JUMMEC.VOL22NO2.4","DOIUrl":"https://doi.org/10.22452/JUMMEC.VOL22NO2.4","url":null,"abstract":"Cryptococcus neoformans is an encapsulated fungal pathogen that caused severe disease primarily in immunocompromised patients. Adherence and internalization of microbial pathogens into host cells are often begin with engagement of microbes to the surface receptors of host. However, the mechanisms involved remain poorly understood. In this study, we investigated association of cell surface determinants of C. neoformans with mammalian cells. Our results showed that treatment with trypsin, but not paraformaldehyde or heat killing, has reduced host-cryptococci interaction, suggesting the involvement of cell surface proteins (CSPs) of C. neoformans in this interaction. We extended our investigations to determine roles of CSPs during cryptococci-host cells interaction by extracting and conjugating CSPs of C. neoformans to latex beads. Conjugation of CSPs with both encapsulated and acapsular C. neoformans increased the association of latex beads with mammalian alveolar epithelial cells, alveolar macrophages and monocyte-derived macrophages. Further examination on the actin organisation of the host cells implied the involvement of actin-dependent phagocytosis in the internalisation of C. neoformans in CSP-conjugated latex beads. We hypothesized that CSPs present on the cell wall of C. neoformans mediate the adherence and actin-dependent phagocytosis of cryptococci by mammalian cells. Our results warrant further studies on the exact role of CSPs in pathogenesis during cryptococcosis.","PeriodicalId":39135,"journal":{"name":"Journal of the University of Malaya Medical Centre","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46380848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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