IF 4.3 3区生物学

Methods Pub Date : 2025-08-27 DOI: 10.1016/j.ymeth.2025.08.011

Minsoo Kim , Su A Kim , Jeong Min Kim , Hee Young Kim , Ho Yeong Yoon , Sung Won Park , Daegyun Park , Ji Sook Han , Ki Tae Suk

{"title":"Validation of combo ichroma as a reliable concentration-based alternative for AST and ALT measurement in liver disease monitoring","authors":"Minsoo Kim , Su A Kim , Jeong Min Kim , Hee Young Kim , Ho Yeong Yoon , Sung Won Park , Daegyun Park , Ji Sook Han , Ki Tae Suk","doi":"10.1016/j.ymeth.2025.08.011","DOIUrl":"10.1016/j.ymeth.2025.08.011","url":null,"abstract":"<div><h3>Background</h3><div>Traditional assays for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) measure enzymatic activity, which degrades during long-term frozen storage, threatening the accuracy of the assay. Instead, Combo ichroma (CI), a fluorescence immunoassay that quantifies AST and ALT concentrations, is a robust alternative for retrospective and point-of-care liver function testing, free from the influence of long-term storage.</div></div><div><h3>Methods</h3><div>Serum samples from 256 individuals (controls and patients with hepatitis, cirrhosis, or liver cancer) were collected and stored at −80 °C for an average of three years. AST and ALT were measured using CI, the 7180 clinical analyzer (HT), and Atellica CH 930 analyzer performed immediately after collection (HL). Correlations between AST, ALT, and AST/ALT ratios were analyzed. Random Forest Regression (RFR) models using CI or HT data were developed to predict HL-derived AST/ALT ratios.</div></div><div><h3>Results</h3><div>CI-AST showed strong correlation with HL-AST across all groups (R2 > 0.95), outperforming HT-AST, especially in controls (R2 = 0.58). CI-ALT moderately correlated with HL-ALT (R2 = 0.87), surpassing HT-ALT (R2 = 0.71). AST/ALT ratios varied across methods due to ALT variability, but RFR using CI data accurately predicted HL ratios (R2 = 0.85–0.91). Subgroup analysis confirmed CI’s superior concordance across etiologies.</div></div><div><h3>Conclusions</h3><div>CI enables activity-independent, reliable measurement of AST and ALT even after extended storage, outperforming enzymatic assays in precision and correlation. Its simplicity, and compatibility with machine learning models position CI as a promising tool for liver enzyme diagnostics in both clinical and resource-limited settings.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 66-75"},"PeriodicalIF":4.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized CUT&Tag enables robust epigenome profiling in Schizosaccharomyces pombe 优化的CUT&Tag能够在裂糖菌pombe中实现稳健的表观基因组分析

IF 4.3 3区生物学

Methods Pub Date : 2025-08-22 DOI: 10.1016/j.ymeth.2025.08.010

Cheng-Zhi Huang , Kang-Di Zhou , Wei Ma

{"title":"Optimized CUT&Tag enables robust epigenome profiling in Schizosaccharomyces pombe","authors":"Cheng-Zhi Huang , Kang-Di Zhou , Wei Ma","doi":"10.1016/j.ymeth.2025.08.010","DOIUrl":"10.1016/j.ymeth.2025.08.010","url":null,"abstract":"<div><div>We optimized permeabilization for CUT&Tag in <em>S. pombe</em>, enabling robust H3K9me3 profiling using lightly fixed permeabilized sepheroplasts, overcoming limitations of ChIP-seq including crosslinking artifacts and high cell input. We established an optimized Cleavage Under Targets and Tagmentation (CUT&Tag) protocol for high-resolution epigenome profiling inusing Critical permeabilization refinements identified Lywallzyme as the optimal enzyme for spheroplast generation (>95 % efficiency in 60 min at 10 mg/mL), outperforming Zymolyase-20 T and combinatorial treatments. Systematic parameter optimization revealed concentration-dependent digestion kinetics and an inverse cell load-efficiency relationship (5 × 10<sup>5</sup> cells achieving > 90 % conversion in 50 min at 5 mg/mL). Validated through H3K9me3 mapping in wild-type and <!-->strains (10⁶ cells/replicate), this approach captured specific heterochromatic enrichment at centromeres/telomeres with complete signal ablation in mutants, while reduced spike-in DNA (0.2 pg) significantly enhanced signal-to-noise ratios. The protocol enables robust epigenomic analysis with minimal cell input and enhanced resolution.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 49-53"},"PeriodicalIF":4.3,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and α-tocopherol analysis in a clinical setting 体积干燥血斑微采样：一个可持续的，病人友好的，实用的方法视黄醇和α-生育酚分析在临床设置

IF 4.3 3区生物学

Methods Pub Date : 2025-08-22 DOI: 10.1016/j.ymeth.2025.08.006

Chaweewan Suwanvecho , Lenka Kujovská Krčmová , František Švec

{"title":"Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and α-tocopherol analysis in a clinical setting","authors":"Chaweewan Suwanvecho , Lenka Kujovská Krčmová , František Švec","doi":"10.1016/j.ymeth.2025.08.006","DOIUrl":"10.1016/j.ymeth.2025.08.006","url":null,"abstract":"<div><div>Dried blood spot (DBS) technique has gained significant attention due to the growth of decentralized diagnostics. This technique reduces the number of hospital visits for patients and the workload for personnel in specialized hospitals. This microsampling method provides an environmentally friendly (green) and patient-friendly alternative to conventional phlebotomy. Challenges related to sample heterogeneity in traditional DBS cards have been overcome by the volumetric DBS sampling using new types of commercially available devices. Due to the unstable nature of the analytes, commercial volumetric DBS devices allow blood sampling at primary care units in remote settings and facilitate transport it via temperature-controlled systems. Blood sample stability has improved from 24 h at 4–8 °C to 30 days at −80°C. DBS also requires over 1000 times less shipping and storage space than liquid blood. We optimized the DBS method to require only 10 µL of blood and achieve extraction efficiencies of over 90 % for retinol when the result from validated method is the reference value. However, α-tocopherol recovery varied from 53 to 75 % depending on the filter paper type used. Furthermore, we successfully developed a liquid–liquid extraction method for both analytes from whole blood, with over 90 % recovery. Our approaches eliminate the need for separate serum and erythrocyte extractions, simplify sample preparation, and reduce reagent use and energy consumption. Both devices enable reliable volumetric collection. Our approach makes micronutrient monitoring more accessible and enables sample collection in decentralized settings. This aligns with the objectives of green analytical chemistry and universal health coverage.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 40-48"},"PeriodicalIF":4.3,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coffee – a ubiquitous substitute for uranyl acetate in staining of biological ultrathin sections for electron microscopy studies 咖啡-在电子显微镜研究的生物超薄切片染色中，醋酸铀酰的普遍替代品

IF 4.3 3区生物学

Methods Pub Date : 2025-08-21 DOI: 10.1016/j.ymeth.2025.08.009

Claudia Mayrhofer , Robert Zandonella , Willi Salvenmoser , Ilse Letofsky-Papst

{"title":"Coffee – a ubiquitous substitute for uranyl acetate in staining of biological ultrathin sections for electron microscopy studies","authors":"Claudia Mayrhofer , Robert Zandonella , Willi Salvenmoser , Ilse Letofsky-Papst","doi":"10.1016/j.ymeth.2025.08.009","DOIUrl":"10.1016/j.ymeth.2025.08.009","url":null,"abstract":"<div><div>The use of uranyl acetate, a staining agent successfully used for decades in electron microscopy of biological specimens, is now strictly regulated by law due to its toxicity and radioactivity. It is even banned in some laboratories. In the meantime, there are a number of substitutes on the market, none of which comes close to the very good staining results of uranyl acetate, or only partially, and some of which are also toxic. In this paper, two alternatives to uranyl acetate are presented, namely coffee, which is used in countless households, and pure chlorogenic acid, which is a component of coffee. We used the well-known zebrafish as biological test material, focusing on the mitochondrial membranes. The staining ability of coffee and chlorogenic acid compared with commercially available staining agents as well as uranyl acetate is assessed by the interference contrast between membranes and their environment. This work also describes how a subjective impression of good or bad contrast can be cast into an objective and comparable numerical value.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 76-82"},"PeriodicalIF":4.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting Anisotropic Orientation in Nanoparticle-Aided Cryo-Electron Microscopy Sampling 利用纳米粒子辅助低温电子显微镜取样的各向异性取向

IF 4.3 3区生物学

Methods Pub Date : 2025-08-21 DOI: 10.1016/j.ymeth.2025.08.008

Yeeun Kim , Changin Kim , Yongsoo Yang , Hyotcherl Ihee

{"title":"Exploiting Anisotropic Orientation in Nanoparticle-Aided Cryo-Electron Microscopy Sampling","authors":"Yeeun Kim , Changin Kim , Yongsoo Yang , Hyotcherl Ihee","doi":"10.1016/j.ymeth.2025.08.008","DOIUrl":"10.1016/j.ymeth.2025.08.008","url":null,"abstract":"<div><div>Measuring the conformational distribution of small proteins is essential to understanding their role in biological systems. Multi-Tilt Nanoparticle-aided cryo-electron microscopy sampling (MT-NACS) was devised to measure the three-dimensional interparticle distance distribution (<em>P</em>(<em>d</em>)) of two gold nanoparticles (AuNPs) labeled on a protein by taking cryogenic electron microscopy (cryo-EM) images at multiple-tilt angles. However, tracking the same particles in a pseudo-tomographic manner during the multi-tilt cryo-EM experiments and data analysis requires extensive time and effort. Here, we report that proper incorporation of AuNP pair angle distribution allows reliable determination of the <em>P</em>(<em>d</em>) only using the cryo-EM images collected at a single tilt angle. The trends of structural changes in calmodulin (CaM) measured by single-tilt angle NACS (ST-NACS) and MT-NACS are consistent, as the tendencies of changes in the <em>P</em>(<em>d</em>) of AuNP-labeled CaM measured by both methods are similar. Our approach provides an efficient tool for measuring the conformational distribution and structural transition of small proteins.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 54-65"},"PeriodicalIF":4.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR-generated DNA templates enable efficient, rapid, and cost-effective mRNA synthesis pcr生成的DNA模板能够高效、快速、经济地合成mRNA。

IF 4.3 3区生物学

Methods Pub Date : 2025-08-16 DOI: 10.1016/j.ymeth.2025.08.007

Naveen Kumar , Nicholas C. Hazell , Jiani Bei , Tina Nguyen , Haitao Hu

{"title":"PCR-generated DNA templates enable efficient, rapid, and cost-effective mRNA synthesis","authors":"Naveen Kumar , Nicholas C. Hazell , Jiani Bei , Tina Nguyen , Haitao Hu","doi":"10.1016/j.ymeth.2025.08.007","DOIUrl":"10.1016/j.ymeth.2025.08.007","url":null,"abstract":"<div><div><em>In vitro</em> transcription (IVT) is a widely used technique for mRNA synthesis in both basic research and the development mRNA-based vaccines and therapies. The efficiency of IVT critically depends on the quality and integrity of the linear DNA templates. The conventional method for template DNA preparation involves plasmid propagation in bacteria followed by enzymatic linearization, which is labor-intensive and costly. Here, we describe a cell-free, PCR-based approach for generating high-quality, high-yield linear DNA templates. We extensively compared the PCR-based method with the conventional plasmid-based approach in terms of IVT efficiency, mRNA production, and the immunogenicity of the resulting mRNA-LNP (lipid nanoparticle) vaccines. Compared to the plasmid-derived DNA, the PCR-based method yielded higher amounts of both DNA templates and transcribed mRNA, while maintaining mRNA quality and integrity. Importantly, mRNA-LNP vaccines encoding the SARS-CoV-2 spike protein, generated from both methods, elicited robust and comparable immune responses in mice, with no significant differences observed between the two template methods. Our findings highlight the advantages of PCR-generated DNA templates as a rapid, efficient, and cost-effective alternative for mRNA synthesis, with broad applications in vaccine and therapeutic development.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 31-39"},"PeriodicalIF":4.3,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights of Surface Enhancing Raman Spectroscopy for Biomedical Application 表面增强拉曼光谱在生物医学上的应用。

IF 4.3 3区生物学

Methods Pub Date : 2025-08-12 DOI: 10.1016/j.ymeth.2025.08.005

Neha Sharma , Anita Singh , Ratneshwar Kumar Ratnesh , Archana Adhana , Lalita Tyagi , Jay Singh

{"title":"Insights of Surface Enhancing Raman Spectroscopy for Biomedical Application","authors":"Neha Sharma , Anita Singh , Ratneshwar Kumar Ratnesh , Archana Adhana , Lalita Tyagi , Jay Singh","doi":"10.1016/j.ymeth.2025.08.005","DOIUrl":"10.1016/j.ymeth.2025.08.005","url":null,"abstract":"<div><div>Raman spectroscopy is a powerful, non-invasive analytical technique that enables rapid identification of molecules based on their unique spectral fingerprints. Its sensitivity has been significantly enhanced through the use of metal nanoparticles in Surface-Enhanced Raman Spectroscopy (SERS), where molecules adsorbed on rough metallic surfaces or colloids produce Raman signals amplified by several orders of magnitude. This enhancement has opened new possibilities for molecular detection, particularly in surface chemistry and biomedical diagnostics.</div><div>In clinical applications, timely and accurate diagnosis is critical, yet conventional bioanalytical methods often require multiple biochemical tests, leading to delays that are especially problematic in emergency settings. SERS provides a promising alternative, offering high sensitivity, specificity, and rapid analysis with minimal sample preparation.</div><div>This review explores the integration of Raman spectroscopy—especially SERS—for both <em>in vivo</em> and <em>ex vivo</em> biomedical diagnostics. It covers sample preparation techniques, spectral data interpretation, and the correlation of Raman signals with disease-specific biomarkers. Special focus is given to the application of Raman-based methods in diagnosing brain disorders, various cancers, drug abuse, and COVID-19. Finally, the article discusses future prospects and challenges to guide the continued advancement of biomedical Raman technologies.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 16-30"},"PeriodicalIF":4.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF-CRIB: A 3D-printable device to facilitate immunofluorescence experiments and its application in screening and characterizing cells expressing a degradable form of ERK2 IF-CRIB：一种3d打印设备，用于促进免疫荧光实验，并用于筛选和表征表达可降解形式ERK2的细胞。

IF 4.3 3区生物学

Methods Pub Date : 2025-08-09 DOI: 10.1016/j.ymeth.2025.08.004

Elsa Berliocchi , Cercina Onesto , Gilles Pagès , Philippe Lenormand , Roser Buscà

{"title":"IF-CRIB: A 3D-printable device to facilitate immunofluorescence experiments and its application in screening and characterizing cells expressing a degradable form of ERK2","authors":"Elsa Berliocchi , Cercina Onesto , Gilles Pagès , Philippe Lenormand , Roser Buscà","doi":"10.1016/j.ymeth.2025.08.004","DOIUrl":"10.1016/j.ymeth.2025.08.004","url":null,"abstract":"<div><div>Immunofluorescence-based detection of proteins in fixed cells is a powerful tool for research in cell and developmental biology. While a variety of immunofluorescence protocols exist, they can be time consuming or require expensive equipment which may not be accessible to all laboratories. A common challenge in these protocols is the numerous washing steps, particularly in experiments with numerous conditions. To address this, here we introduce the IF-CRIB device, a 3D-printable wash rack specifically designed for applications involving a high number of round coverslips with adherent cultured cells. We detail its design and the 3D printing process which can be easily used by any laboratory and we highlight that it facilitates the numerous washing steps. In addition, we present the IF-Express protocol, an optimized and effective method that enables fast and consistent immunofluorescence results. As an example of the utility of the IF-CRIB device and the IF-Express protocol, we describe their application in the screening and characterization of several NIH3T3 cell clones expressing a degradable form of ERK2 kinase (ERK2-dTAG) after treatment with the dTAG-13 compound. The generation of ERK2-dTAG clones involves a <em>knock-in</em> strategy. We provide a detailed methodology for clone selection, immunofluorescence screening, and characterization of ERK2-dTAG, including degradation kinetics, dose–response analysis, and nuclear translocation assays to assess ERK2-dTAG functionality. The IF-CRIB device and IF-Express protocol has been proven to be efficient for the obtention and characterization of ERK2dTAG-expressing clones thereby offering a powerful framework for studying ERK2 dynamics in cell biology and disease models.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"243 ","pages":"Pages 1-15"},"PeriodicalIF":4.3,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of immunoregulatory peptides from gut microbiota modulating Epstein-Barr virus-induced gene 3 (EBI3) using a novel specific AlphaLISA™ assay 使用一种新的特异性AlphaLISA™检测方法鉴定肠道微生物群中调节eb病毒诱导基因3 （EBI3）的免疫调节肽

IF 4.3 3区生物学

Methods Pub Date : 2025-08-05 DOI: 10.1016/j.ymeth.2025.08.002

Sothea Touch , Maria Lucia Orsini Delgado , Parfait Evouna-Mengue , Lydie Surand , Souphalone Luangsay , Naeemunnisa Mohamed Anesary , Francesco Strozzi , Laurent Chêne , Antonietta Cultrone

{"title":"Identification of immunoregulatory peptides from gut microbiota modulating Epstein-Barr virus-induced gene 3 (EBI3) using a novel specific AlphaLISA™ assay","authors":"Sothea Touch , Maria Lucia Orsini Delgado , Parfait Evouna-Mengue , Lydie Surand , Souphalone Luangsay , Naeemunnisa Mohamed Anesary , Francesco Strozzi , Laurent Chêne , Antonietta Cultrone","doi":"10.1016/j.ymeth.2025.08.002","DOIUrl":"10.1016/j.ymeth.2025.08.002","url":null,"abstract":"<div><div>Gut microbiota-derived compounds are pivotal in modulating host immunity by regulating the functions of various key innate and adaptive immune cells. Epstein-Barr virus-induced gene 3 (EBI3) serves as the beta subunit shared by the heterodimeric cytokines interleukin (IL)-27 and IL-35. Both these cytokines have been documented to inhibit the development of T helper 2 (Th2) and T helper 17 (Th17) cells, while enhancing the function of regulatory T cells (Tregs). EBI3, itself, has also been shown to regulate cell-mediated immune responses. Despite their critical roles in maintaining immune homeostasis, there is a significant lack of robust, high-throughput-compatible assays to evaluate the secretion of IL-27, IL-35, or EBI3.</div><div>In this study, we detail the development of a novel amplified luminescent proximity homogeneous assay (AlphaLISA™) to quantify EBI3 secretion by tolerogenic dendritic cells. We utilized this assay to screen a library of 9739 small proteins derived from the human gut microbiota to identify compounds that could stimulate EBI3 secretion.</div><div>Our findings revealed the immunoregulatory potential of VAC18, an unknown protein from <em>Fusicatenibacter saccharivorans</em> (Clostridiumcluster XIVa) which significantly induces the secretion of both EBI3 and IL-27. This is the first study to demonstrate the effect of gut microbiota derived peptides on the balanced secretion of EBI3 and IL-27.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"242 ","pages":"Pages 148-158"},"PeriodicalIF":4.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biosensors: structure, principles, classification, and application in bio-manufacturing 生物传感器的研究进展：结构、原理、分类及其在生物制造中的应用。

IF 4.3 3区生物学

Methods Pub Date : 2025-08-05 DOI: 10.1016/j.ymeth.2025.07.011

Weiguang Gao , Qi Wang , Weili Gong , Lan Zheng , Qingai Liu , Lihe Zhang , Yaohong Ma

{"title":"Recent advances in biosensors: structure, principles, classification, and application in bio-manufacturing","authors":"Weiguang Gao , Qi Wang , Weili Gong , Lan Zheng , Qingai Liu , Lihe Zhang , Yaohong Ma","doi":"10.1016/j.ymeth.2025.07.011","DOIUrl":"10.1016/j.ymeth.2025.07.011","url":null,"abstract":"<div><div>Bio-manufacturing, as a frontier field of the new round of global scientific and technological revolution and industrial change, is becoming a core driving force for the future development of the bioeconomy. However, the bio-manufacturing process itself contains complex metabolic mechanisms and fine process control, which undoubtedly increases its technical difficulty. Especially at a time when the field of intelligent bio-manufacturing is developing rapidly, more stringent requirements have been placed on the ability to sense key biochemical information during the bio-manufacturing process. Biosensors based on biomolecular recognition provide powerful technical support for real time monitoring and precise control of key biochemical information that can rapidly sense the production process, and their applications have made remarkable progress. This article reviews the structure, classification, and working principle of biosensors and discusses their applications in bio-manufacturing, such as real-time monitoring of key biochemical parameters, intracellular and extracellular metabolite concentrations, and high-throughput screening for precise control and optimization of bioprocesses. The article also analyses the challenges facing biosensors, including the need for stability and reliability enhancements, and future directions. Researchers are developing new biometric components and sensor materials with advanced signal conversion techniques, while microelectronics and nanotechnology are driving the miniaturization and integration of sensors. These advances are expected to make biosensors more useful in microbial fermentation and biotechnology.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"242 ","pages":"Pages 123-142"},"PeriodicalIF":4.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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