Journal of Biomechanical Science and Engineering最新文献_第2页

Advancing FRAP for cell studies: Where there is a new method, there is a new field 推进FRAP用于细胞研究:有了新方法，就有了新领域

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00028

Takumi Saito, S. Deguchi

引用次数: 0

Mechano-neurophysiological model of fingertip to simulate tactile response during Braille reading under multiple frictional conditions 模拟多种摩擦条件下盲文阅读时指尖触觉反应的机械神经生理模型

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00102

Toru HAMASAKI, Yuko NAKAHIRA, Daisuke YAMADA

{"title":"Mechano-neurophysiological model of fingertip to simulate tactile response during Braille reading under multiple frictional conditions","authors":"Toru HAMASAKI, Yuko NAKAHIRA, Daisuke YAMADA","doi":"10.1299/jbse.23-00102","DOIUrl":"https://doi.org/10.1299/jbse.23-00102","url":null,"abstract":"The evaluation of tactile sensitivity involving frictional behavior via computational models can further accelerate the development of industrial products in terms of usability. This study aimed to confirm the capability of a previously proposed mechano-neurophysiological model, representing the basic mechanical (i.e., finger skin deformation) and neurophysiological (i.e., neural activities of slowly adapting type-1 (SA1) afferents) functions in the tactile sensation process, for simulating tactile responses during Braille reading under multiple frictional conditions. A previous psychophysical experiment on tactile recognition during Braille reading reported that the misread rate was significantly higher at frictional coefficient (μ) = 2.62 than at μ = 0.56, whereas no significant differences were observed between the misread rates at μ = 0.25 and 0.77. The Braille reading experiment was simulated using the mechano-neurophysiological model to achieve the present aim. The simulation results revealed marginal differences in the SA1 responses between μ = 0.25 and 0.77, and the correlation coefficients between the SA1 responses and Braille patterns were 0.98 at μ = 0.25 and 0.96 at μ = 0.77, suggesting a limited influence of friction on Braille recognition. However, the SA1 responses varied considerably between μ = 0.56 and 2.62, and the correlation coefficients were 0.97 at μ = 0.56 and 0.49 at μ = 2.62, implying a relatively strong influence of friction. The simulation results supported the above-mentioned findings of the previous psychophysical experiment, thereby demonstrating that the mechano-neurophysiological model qualitatively determined the tendency of influence of friction on Braille recognition.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"460 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135560156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Model blood for simulating red thrombus formation owing to stagnant blood flow using hypercoagulable skim milk solution 用高凝脱脂牛奶溶液模拟因血流停滞而形成的红色血栓

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00350

Tsutomu TAJIKAWA, Mitsuru HIRONO, Masayuki TANAKA, Ryosuke YANO, Kyoko NAGIRA

{"title":"Model blood for simulating red thrombus formation owing to stagnant blood flow using hypercoagulable skim milk solution","authors":"Tsutomu TAJIKAWA, Mitsuru HIRONO, Masayuki TANAKA, Ryosuke YANO, Kyoko NAGIRA","doi":"10.1299/jbse.23-00350","DOIUrl":"https://doi.org/10.1299/jbse.23-00350","url":null,"abstract":"The thrombus formation process has yet to be fully simulated, which necessitates the design of a novel method for the quantitative assessment of device thrombogenicity that complements animal experiments/ex vivo experiments using animal blood and computational fluid dynamics analysis. This study aimed to develop a model blood capable of simulating the formation of red thrombus owing to stagnation of blood flow by applying the phenomenon of milk clot formation. To render the rheological properties of skim milk solution used as a model blood closer to those of human blood, the ratio of the amounts of casein in skim milk, calcium chloride, and rennet (constituents of the solution of the model blood) was varied and the rheological properties of the clot formation process of human blood and skim milk solution were measured using a cone-plate viscometer. Comparisons of the rheological properties of human blood and skim milk solutions during clotting revealed that during clot formation of human blood, four characteristic quantities of the time-series change in rheological properties were observed: the viscosity before clotting, coagulation start time, viscosity increase rate, and first yield viscosity. Further, hypercoagulable skim milk solutions with rheological properties similar to those of human blood were prepared by adjusting the solution composition ratio. When this model blood was circulated in a closed-loop circuit with a saccular aneurysm model, the growth rate of skim milk clot formation in the aneurysm model was significantly different in a flow diverter stent model and a micro-porous covered stent. The variation in porosity between these two stent models has a direct impact on the rate of embolisation. The proposed blood model can effectively replicate the formation of red thrombus, providing a valuable means to accurately and quantitatively assess the therapeutic efficacy of embolisation devices.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135605086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atomic force microscopy for investigating cell and tissue mechanics as heterogeneous and hierarchical materials 原子力显微镜用于研究细胞和组织力学作为异质和分层材料

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00339

Takaharu OKAJIMA, Kaori KURIBAYASHI-SHIGETOMI

{"title":"Atomic force microscopy for investigating cell and tissue mechanics as heterogeneous and hierarchical materials","authors":"Takaharu OKAJIMA, Kaori KURIBAYASHI-SHIGETOMI","doi":"10.1299/jbse.23-00339","DOIUrl":"https://doi.org/10.1299/jbse.23-00339","url":null,"abstract":"Atomic force microscopy (AFM) has been extensively used to measure the mechanical properties of single cells and tissues with a high force sensitivity. AFM has been established to quantify mechanical differences between cells, e.g., between normal and disease cells, and between untreated (controlled) and treated cells. However, since these biological samples are intrinsically heterogeneous and hierarchical materials, AFM often suffers from the quantification of cell and tissue mechanics due to the high spatial resolution of AFM from the nanoscale to the microscale, comparable to the spatial variation and fluctuation of living systems. Thus, it is still challenging to elucidate universal nano- and micro-mechanical features of living systems using AFM data. This review addresses how AFM can quantify the heterogeneities and hierarchies of cell systems. For single-cell mechanical analysis, AFM has been combined with micropatterned substrate to control cell shape and precisely define the AFM measurement within cells, allowing us to analyze the cell-to-cell mechanical variation. For tissue mechanical analysis, we introduce AFM with a wide-scan range to map multicellular samples from a few hundred to millimeter scales, depending on the type of scanner, allowing us to quantify the spatial mechanical variation in multicellular systems. The reliability and the possibility of AFM to apply mechanics studies on cells and tissues with a range of Pascal (Pa) to MPa are addressed.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136306833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement in the precision of capillary refill time measurements for diagnosing hypovolemic status 提高诊断低血容量状态的毛细血管再充血时间测量的精度

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00002

Shukei Sugita, Takato Mizuno, Y. Ujihara, Masanori Nakamura

引用次数: 0

Extracting quantitative relationships between cell motility and molecular activities (Analytical approaches and implications) 提取细胞运动和分子活动之间的定量关系(分析方法和意义)

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00336

Yuichi SAKUMURA, Katsuyuki KUNIDA

{"title":"Extracting quantitative relationships between cell motility and molecular activities (Analytical approaches and implications)","authors":"Yuichi SAKUMURA, Katsuyuki KUNIDA","doi":"10.1299/jbse.23-00336","DOIUrl":"https://doi.org/10.1299/jbse.23-00336","url":null,"abstract":"Despite considerable advancements in biological measurement technologies, capturing the simultaneous temporal changes in various biomolecular concentrations remains a challenge. Overcoming this technical difficulty via data preprocessing could not only clarify the principles of biological functions but also reduce the costs associated with advancing measurement technologies. This review introduces a novel approach to harmonizing heterogeneous time-series data related to molecular signaling and cellular movement. In response to this challenge, we developed and employed a motion-trigger average (MTA) algorithm. The MTA comprehensively screens and averages intracellular molecular activities that coincide with targeted velocity patterns of the moving cell edge. Given that the MTA filters out cell individuality-dependent noise from the data, a straightforward regression equation can correlate edge moving velocities with the molecular activities of various species within the cell. This methodology not only integrates fragmented datasets but also enables the reuse of past data for new analyses. The crux of our discovery is the elucidation of the role that Rho GTPases play in regulating cellular edge dynamics, a finding made possible by adopting the MTA algorithm. Our study suggests that the MTA could become an indispensable tool in data-driven biology, potentially paving the way for considerable insights into dynamic cellular behaviors and the underlying biological principles.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136304442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monocular camera-based 3D human body pose estimation by Generative Adversarial Network considering joint range of motion represented by quaternion 基于四元数关节运动范围生成对抗网络的单目摄像机三维人体姿态估计

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.22-00305

Akisue Kuramoto, Kosuke Mizukoshi, M. Nakashima

引用次数: 0

The effect of stiff substrates on the collective migration of A549 cells 硬底物对A549细胞集体迁移的影响

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00298

Saori SASAKI, Zhaoyi DU, Ryu TAKAHASHI, Qi SUN, Toshihiro SERA, Susumu KUDO

{"title":"The effect of stiff substrates on the collective migration of A549 cells","authors":"Saori SASAKI, Zhaoyi DU, Ryu TAKAHASHI, Qi SUN, Toshihiro SERA, Susumu KUDO","doi":"10.1299/jbse.23-00298","DOIUrl":"https://doi.org/10.1299/jbse.23-00298","url":null,"abstract":"The Young’s modulus of normal lung tissues ranges from 1 to 5 kPa, whereas fibrotic or lung tumor tissues can be ~30 times stiffer. However, the lung parenchyma microscopically consists of various tissues, and cancer cells in tumors contact stiff collagen-containing fibers during invasion. In this study, we investigated the effect of stiff substrates on A549 cell migration. We fabricated stiff polydimethylsiloxane (PDMS) substrates with different stiffness levels (1.4, 3.4, and 18.3 MPa) by adjusting the cross-linking agent ratio. We examined the distance and the trajectory orientation of A549 cell migration on PDMS substrates and much stiffer 7.7 GPa glass with scratch-wound assays. A549 cells exhibited a collective sheet-like migration pattern toward the wounded area on stiff substrates. The migration range at 18.3 MPa was significantly higher than at 1.4 and 3.4 MPa, and that on glass was significantly higher than that at 18.3 MPa. The cell trajectory orientation on stiff PDMS substrates gradually increased and became constant, whereas that on glass was constant from the initial migration. Our results revealed that stiff substrates affect A549 cells migration in this range.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135755112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of ground reaction forces and moments and joint kinematics and kinetics by inertial measurement units using 3D forward dynamics model 利用三维正演动力学模型预测惯性测量单元的地面反作用力和力矩以及关节运动学和动力学

Journal of Biomechanical Science and Engineering Pub Date : 2023-01-01 DOI: 10.1299/jbse.23-00130

Naoto HARAGUCHI, Kazunori HASE

{"title":"Prediction of ground reaction forces and moments and joint kinematics and kinetics by inertial measurement units using 3D forward dynamics model","authors":"Naoto HARAGUCHI, Kazunori HASE","doi":"10.1299/jbse.23-00130","DOIUrl":"https://doi.org/10.1299/jbse.23-00130","url":null,"abstract":"Predicting the ground reaction force (GRF) and ground reaction moment (GRM) with a biomechanical model-based approach has an advantage for biomechanical gait analysis in situations where a statistical model cannot be used due to a lack of training data. However, the current prediction methods using a biomechanical model have some issues for clinical application. The present study developed a new inertial measurement unit (IMU)-based method to predict the GRF, the GRM, and the joint kinematics and kinetics with a 3D biomechanical model and simple system. The present method predicts them using a 3D forward dynamics model that computationally generates human movements that minimize the hybrid cost function defined by physical loads and errors between the motion of the model and that of the participants recorded by six IMUs, which allows the prediction system to use only a relatively small number of IMUs. We investigated the prediction accuracy during walking by comparing the new method with a conventional analysis using a force plate and motion capture system. As a result, we observed strong and excellent correlations between the prediction and measurement of the anterior GRF, vertical GRF, sagittal GRM, hip flexion angle, knee flexion angle, hip flexion torque, and ankle dorsiflexion torque. Considering the accuracy of previous studies and that required for gait analysis, the present method could predict them with practical accuracy due to estimated biomechanically valid motions based on optimization using the hybrid cost function that includes a biomechanical evaluation. Moreover, the prediction system has an advantage for clinical applications because the present method observed practical accuracy that has the potential to be applied to some sports analysis and can analyze 3D motion with a simple system consisting of a small number of hardware components and a software.","PeriodicalId":39034,"journal":{"name":"Journal of Biomechanical Science and Engineering","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135443085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of mechanosensing and mechanoresponse mechanisms in osteoblasts and osteocytes: in vitro experiments targeting subcellular components 成骨细胞和骨细胞的机械感应和机械反应机制的研究:针对亚细胞成分的体外实验

Journal of Biomechanical Science and Engineering Pub Date : 2022-01-01 DOI: 10.1299/jbse.22-00267

Nobuhiko Nakao, Taiji Adachi

引用次数: 0