Korean Journal of Veterinary Research最新文献_第3页

Anticancer effect of metformin alone and in combination with 2-deoxy-D-glucose on mouse T cell lymphoma EL4 cells 二甲双胍单用及联合2-脱氧-d -葡萄糖对小鼠T细胞淋巴瘤EL4细胞的抗癌作用

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230026

Si-Yeon Kim, Hong-Gu Joo

{"title":"Anticancer effect of metformin alone and in combination with 2-deoxy-D-glucose on mouse T cell lymphoma EL4 cells","authors":"Si-Yeon Kim, Hong-Gu Joo","doi":"10.14405/kjvr.20230026","DOIUrl":"https://doi.org/10.14405/kjvr.20230026","url":null,"abstract":"Metformin is a treatment used widely for non-insulin-dependent diabetes mellitus with few side effects and acts by inhibiting hepatic gluconeogenesis and glucose absorption from the gastrointestinal tract. Lymphoma is one of the most common hematological malignancies in dogs. Chemotherapy is used mainly on lymphoma, but further research on developing anticancer drugs for lymphoma is needed because of its severe side effects. This study examined the anticancer effects of metformin alone and in combination with 2-deoxy-D-glucose (2-DG), a glucose analog, on EL4 cells (mouse T-cell lymphoma). Metformin reduced the metabolic activity of EL4 cells and showed an additive effect when combined with 2-DG. In addition, cell death was confirmed using a trypan blue exclusion test, Hochest 33342/propidium iodide (PI) staining, and Annexin V/PI staining. An analysis of the cell cycle and mitochondria membrane potential (MMP) to investigate the mechanism of action showed that metformin stopped the G2/M phase of EL4 cells, and metformin + 2-DG decreased MMP. Metformin exhibited anticancer effects as a G2/M phase arrest mechanism in EL4 cells and showed additive effects when combined with 2-DG via MMP reduction. Unlike cytotoxic chemotherapeutic anticancer drugs, metformin and 2-DG are related to cellular glucose metabolism and have little toxicity. Therefore, metformin and 2-DG can be an alternative to reduce the toxicity caused by chemotherapeutic anticancer drugs. Nevertheless, research is needed to verify the in vivo efficacy of metformin and 2-DG before they can be used in lymphoma treatments.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful treatment of a severe open wound in a raccoon dog (Nyctereutes procyonoides): antibiotic susceptibility testing supported 成功治疗一只浣熊(原yonoides)的严重开放性伤口:支持抗生素敏感性试验

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230028

Myeongsu Kim, Haerin Rhim, Seulgi Gim, Chang-Eun Lee, Hakyoung Yoon, Jae-Ik Han

引用次数: 0

Antioxidant and anti-inflammatory activities of Lespedeza cuneata in Coal fly ash-induced murine alveolar macrophage cells 胡枝子对粉煤灰诱导小鼠肺泡巨噬细胞的抗氧化和抗炎活性

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230022

Abdul Wahab, Hwayong Sim, Kyubin Choi, Yejin Kim, Yookyeong Lee, Byungwook Kang, Yu Seong No, Dongyeop Lee, Inseo Lee, Jaehyeon Lee, Hwajun Cha, Sung Dae Kim, Evelyn Saba, Man Hee Rhee

{"title":"Antioxidant and anti-inflammatory activities of Lespedeza cuneata in Coal fly ash-induced murine alveolar macrophage cells","authors":"Abdul Wahab, Hwayong Sim, Kyubin Choi, Yejin Kim, Yookyeong Lee, Byungwook Kang, Yu Seong No, Dongyeop Lee, Inseo Lee, Jaehyeon Lee, Hwajun Cha, Sung Dae Kim, Evelyn Saba, Man Hee Rhee","doi":"10.14405/kjvr.20230022","DOIUrl":"https://doi.org/10.14405/kjvr.20230022","url":null,"abstract":"Lespedeza cuneata (LC) is a perennial plant used in herbal medicine to treat numerous diseases, including prostatic hyperplasia, diabetes, early atherosclerosis, and hematuria. Reference collections of bioactive compounds of LC are crucial for the determination of their pharmacological properties. However, little is known regarding its anti-oxidative and anti-inflammatory effects in alveolar macrophage (MH-S) cells. This study examined whether LC can inhibit reactive oxygen species and Coal fly ash (CFA) induced inflammation in MH-S cells. The anti-oxidative effects of LC were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, anti-inflammatory effects were examined using nitric oxide (NO) assay, and cytotoxicity was analyzed using methyl thiazolyl tetrazolium assay. The expression of inflammatory cytokine genes was assessed through a reverse-transcription polymerase chain reaction. Our results revealed that LC exhibited high radical scavenging activity and a dose-dependent (7.8–1,000 μg/mL) inhibition of oxidation as compared to ascorbic acid and Trolox. It also inhibited CFA-induced NO production in MH-S cells. Moreover, it suppressed the CFA exposure-mediated expression of pro-inflammatory mediators and cytokines, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. These results suggest that LC is a potent antioxidant and anti-inflammatory agent that can be useful as a nutraceutical product.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forensic analysis of toxic substances in fatalities with suspected companion animal cruelty 对疑似虐待伴侣动物的死亡事件进行有毒物质的法医分析

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230017

Jeong Woo Kang, Ah-Young Kim, Hyun Young Chae, Hanae Lim, Suncheun Kim, Bok-Kyung Ku, Kyunghyun Lee

{"title":"Forensic analysis of toxic substances in fatalities with suspected companion animal cruelty","authors":"Jeong Woo Kang, Ah-Young Kim, Hyun Young Chae, Hanae Lim, Suncheun Kim, Bok-Kyung Ku, Kyunghyun Lee","doi":"10.14405/kjvr.20230017","DOIUrl":"https://doi.org/10.14405/kjvr.20230017","url":null,"abstract":"The increasing prevalence of toxic substance-exposure in pets in South Korea endangers the health and safety of numerous companion animals, and has become a cause for concern. Notably, the annual incidence of forensic analysis in pets has increased by more than 150% in South Korea, mainly in populous regions such as Seoul, Incheon, and Gyeonggi. In response to this growing issue, veterinary forensic examinations were conducted on 549 dogs and cats from 2019 to 2022. This study revealed the presence of various toxic substances, including pesticides, insecticides, and drugs such as analgesics, anesthetics, antidepressants, and muscle relaxants, in pets. Among the 38 different toxins identified in pets, coumatetralyl, methomyl, terbufos, and buprofezin were the most frequently detected. In this study, toxic substances for pets were identified based on the “toxic agent list for humans,” developed by the National Forensic Services, because no list of toxic agents for animals currently exists and data regarding potentially toxic substances for dogs and cats is limited. This is one of the limitations of this study, and necessitates the establishment of a toxic agent list for animals. Continued monitoring and research is also recommended to reveal the incidence, causes, and solutions of toxicity in animals.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic resonance imaging features of syringobulbia in small breed dogs 小品种犬脊髓畸形的磁共振成像特征

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230030

Young-Mok Song, In Lee, Yu-Mi Song, Ho-Jung Choi, Young-Won Lee

引用次数: 0

Immune-mediated hemolysis after administration of human intravenous immunoglobulin in a dog: a case report 人静脉注射免疫球蛋白后犬的免疫介导溶血:一例报告

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230025

Minji Kim, Youngju Kim, Hyeona Bae, Rankyung Jung, Minjeong Kang, Sumin Cha, Kyu-Woan Cho, Dong-In Jung, Do-Hyeon Yu

引用次数: 0

Biomechanical comparison of bone staple techniques for stabilizing tibial tuberosity fractures 钉骨技术稳定胫骨粗隆骨折的生物力学比较

Korean Journal of Veterinary Research Pub Date : 2023-09-30 DOI: 10.14405/kjvr.20230020

Kyu-Tae Park, Min-Yeong Lee, Hwi-Yool Kim

{"title":"Biomechanical comparison of bone staple techniques for stabilizing tibial tuberosity fractures","authors":"Kyu-Tae Park, Min-Yeong Lee, Hwi-Yool Kim","doi":"10.14405/kjvr.20230020","DOIUrl":"https://doi.org/10.14405/kjvr.20230020","url":null,"abstract":"This study compared the biomechanical properties of bone-stapling techniques with those of other fixation methods used for stabilizing tibial tuberosity fractures using 3-dimensionally (3D)-printed canine bone models. Twenty-eight 3D-printed bone models made from computed tomography scan files were used. Tibial tuberosity fractures were simulated using osteotomy. All samples were divided into 4 groups. Group 1 was stabilized with a pin and tension-band wire; group 2, with a pin and an 8 mm-wide bone staple; group 3, with 2 horizontally aligned pins and an 8 mm-wide bone staple; and group 4 with a 10 mm-wide bone staple. Tensile force was applied with vertical distraction until failure occurred. The load and displacement were recorded during the tests. The groups were compared based on the load required to cause displacements of 1, 2, and 3 mm. The maximum failure loads and modes were recorded. The loads at all displacements in group 4 were greater than those in groups 1, 2, and 3. The loads at 1, 2, and 3 mm displacements were similar in groups 1 and 3. There was no significant difference between groups 1 and 3. Groups 1 and 4 provided greater maximum failure loads than groups 2 and 3. Failure occurred because of tearing of the nylon rope, tibial fracture, wire breakage, pin bending, and fracture around the bone staple insertion. In conclusion, these results demonstrate that the bone-stapling technique is an acceptable alternative to tension-band wire fixation for the stabilization of tibial tuberosity fractures in canine bone models.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental infection of a porcine kidney cell line with hepatitis A virus 甲型肝炎病毒感染猪肾细胞系的实验研究

Korean Journal of Veterinary Research Pub Date : 2023-06-30 DOI: 10.14405/kjvr.20230019

Dong-Hwi Kim, Da-Yoon Kim, Jae-Hyeong Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, C. Song, Sang-Won Lee, I. Choi

引用次数: 0

Effect of resynchronization programs according to the ovarian status on pregnancy outcomes in dairy cows 奶牛卵巢状态再同步方案对妊娠结局的影响

Korean Journal of Veterinary Research Pub Date : 2023-06-30 DOI: 10.14405/kjvr.20230011

J. Jeong, U. Kim, Illhwa Kim

引用次数: 0

Small intestinal epiploic foramen entrapment in a seven-month pregnant Thoroughbred mare: a case report 怀孕7个月的纯种母马小肠大网膜孔卡压1例

Korean Journal of Veterinary Research Pub Date : 2023-06-30 DOI: 10.14405/kjvr.20230014

Hye-shin Hwang, Seyoung Lee, Kyung-won Park, Eun-bee Lee, Taeyoung Kang, Jong-pil Seo, Hyohoon Jeong

引用次数: 0