BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77306

L. Sharapova, N. Yurina

{"title":"In vitro reconstitution of complexes of stress HliA protein with pigments","authors":"L. Sharapova, N. Yurina","doi":"10.3897/biorisk.17.77306","DOIUrl":"https://doi.org/10.3897/biorisk.17.77306","url":null,"abstract":"Proteins similar to Hli (high light inducible) proteins of cyanobacteria are present in all photosynthetic eukaryotes and are necessary for survival in various stressful conditions, although their exact function is not fully understood. In current study, the recombinant stress-induced protein HliA of cyanobacterium Synechocystis was isolated and characterised for the first time. The synthetic gene of HliA protein was created and cloned into plasmid for expression of recombinant protein with Hisx6-tag at the C-terminus in bacteria. Recombinant HliA protein of Synechocystis was isolated by metal-affinity chromatography. The HliA protein was reconstituted with chlorophyll a and carotenoids. Using circular dichroism spectroscopy, it was shown that chlorophyll a and carotenoids interact in vitro with the HliA protein. The binding of pigments to the HliA protein favours the protective function of this protein. Apparently, Hli proteins are involved in the coordinated delivery of pigments for the biogenesis of photosynthetic complexes, thereby reducing the risk of accumulation of phototoxic free chlorophyll molecules. Current results are important for understanding the processes of photoprotection in either cyanobacteria or algae and higher plants.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43580415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-monitoring geochemical research of the river sediments in the area of Ada Tepe gold mining site (Eastern Rhodopes) Pre-monitoring东罗多彼Ada Tepe金矿矿区河流沉积物地球化学研究

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77466

D. Zhelev, Rumen Penin

{"title":"Pre-monitoring geochemical research of the river sediments in the area of Ada Tepe gold mining site (Eastern Rhodopes)","authors":"D. Zhelev, Rumen Penin","doi":"10.3897/biorisk.17.77466","DOIUrl":"https://doi.org/10.3897/biorisk.17.77466","url":null,"abstract":"The article depicts the geochemical properties of the landscapes in the Ada Tepe gold mine area before its launching. The research is conducted by examining the heavy metals (Cu, Pb, Zn, Co, Cr, Mn and Ni) content in samples of river sediments in the local landscapes. The research aims to analyse the concentration of heavy metals before the launch of gold mining. The study implements the coefficient of Clarke concentration. The deviation from the background concentrations is a ratio between the element concentration in the collected environmental samples and the Clarke value of the element. The coefficient has a scale from 0 to a particular positive value, corresponding to the level of enrichment of the sample in comparison to the background Clarke value. The values corresponding to the Clarke concentration are equal to 1, the lower values are between 0 and 1 (dispersion) and any value higher than 1 is a case of concentration (enrichment). The obtained results display the researched territory as a natural background area. The content of heavy metals in the river sediments of the researched area (mg/kg, median value) by chemical elements is Cu (15), Zn (72), Pb (17), Mn (461), Ni (35), Co (8) and Cr (60). That is the reason it could be defined as not impacted by human activities and it is not influenced by natural geochemical anomalies. Heavy metals do not pollute the researched landscapes before mining. This outcome is obtained by the geochemical content of the investigated heavy metals in the river sediments.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43836588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of proline and methyl jasmonate priming on in vitro seed germination and seedling development of Chelidonium majus L 脯氨酸和茉莉酸甲酯引发对大白菜离体种子萌发和幼苗发育的影响

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77465

Iva Doycheva

{"title":"Influence of proline and methyl jasmonate priming on in vitro seed germination and seedling development of Chelidonium majus L","authors":"Iva Doycheva","doi":"10.3897/biorisk.17.77465","DOIUrl":"https://doi.org/10.3897/biorisk.17.77465","url":null,"abstract":"Drought, salinization and heavy metal pollution of soils are main stress factors with an increasing impact on the deterioration of soil quality, yield and crop quality. Seed priming shows good results in improving seed germination, seedling growth and plant development. Proline (Pro) and metyl jasmonate (MeJA) show stimulating activity and help plants overcome stress. The study investigated the effect of Pro, MeJA and hydropriming on seeds sown on water agar supplemented with different concentrations of heavy metals (Cd, Pb, Zn) (HM), NaCl or Polyethylene glycol 6000 (PEG 6000). Chelidonium majus is a medicinal species which is grown as a crop in some parts of Europe. It is an ingredient in some remedies and is becoming an increasingly popular object of research regarding its biological activities. The low concentrations of all heavy metals applied increased the germination of all variants of seeds – control, hydroprimed and those which were Pro and MeJA primed. Seed priming with Pro and MeJA promoted high germination percentage of seeds germinated on water agar with NaCl. PEG 6000 at its higher concentration (5%) slightly increased the seed germination of all variants. The growth of roots and hypocotyls was inhibited by HM and NaCl. However, PEG 6000 slightly influenced their growth.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49435270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Light and auxin treatments affect morphogenesis and polyphenolics productivity in Artemisia alba Turra cell aggregates in vitro Light和生长素处理对体外培养的黄花蒿细胞聚集体形态发生和多酚类物质生产的影响

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77452

Dobrina Pecheva, K. Danova

{"title":"Light and auxin treatments affect morphogenesis and polyphenolics productivity in Artemisia alba Turra cell aggregates in vitro","authors":"Dobrina Pecheva, K. Danova","doi":"10.3897/biorisk.17.77452","DOIUrl":"https://doi.org/10.3897/biorisk.17.77452","url":null,"abstract":"Artemisia alba Turra is an essential oil-bearing shrub, with a Euro-Mediterranean distribution widespread in the south-eastern parts of Europe. Phytochemical investigations have evidenced the presence of volatile mono- and sesquiterpene derivatives, as well as non-volatile sesquiterpenoids, flavonoids and phenolic acids contributing to the anti-inflammatory, antimicrobial, antioxidant and pro-apoptotic activity of different preparations, obtained from the plant. The current research aims at elucidation of the potential for biotechnological polyphenolic compounds productivity of non-differentiated cell lines of the plant. For this purpose, non-differentiated cell aggregates were initiated from either leaf or root explants of the sterile grown plant. They were cultivated either in the dark or at 16/8 h photoperiod in liquid media, supplemented with N6-benzyladenine (BA) as auxin. The cytokinin effects of indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) were compared. It was established that NAA supplementation was superior to IBA and light treatment – to dark growth conditions in terms of polyphenolics productivity. In addition, NAA supplementation led to better expressed compaction and larger size of the cell aggregates as compared with IBA. The results of the present experiment indicate that secondary metabolites productivity in vitro is a dynamic process closely related to the plant’s growth and development and is in close relation to the interactions of the plant with its environmental conditions.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48300546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal changes in the pro/antioxidant status of mussels Mytilus galloprovincialis (Lamarck, 1819) from Bulgarian Black Sea coastal habitats 保加利亚黑海沿岸栖息地贻贝Mytilus galloprovincialis（Lamarck，1819）促/抗氧化状态的季节变化

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77279

E. Tsvetanova, A. Georgieva, N. Chipev, A. Alexandrova

{"title":"Seasonal changes in the pro/antioxidant status of mussels Mytilus galloprovincialis (Lamarck, 1819) from Bulgarian Black Sea coastal habitats","authors":"E. Tsvetanova, A. Georgieva, N. Chipev, A. Alexandrova","doi":"10.3897/biorisk.17.77279","DOIUrl":"https://doi.org/10.3897/biorisk.17.77279","url":null,"abstract":"The pro/antioxidant status of marine macrozoobentic organisms is being increasingly applied in environmental monitoring and conservation programs. The oxidative stress level in marine bivalves can provide valuable information not only on the health of the organisms and their populations, but also on the current state of habitats and ecosystems. The aim of the present study was to make the first comprehensive investigation of the seasonal changes in the antioxidant activity in different organs (gills, digestive gland and foot) of M. galloprovincialis from representative Bulgarian Black Sea coastal habitats. The lipid peroxidation and glutathione levels, as well as activities of the antioxidant enzymes catalase, superoxide dismutase, glutathion peroxidase, glutathion reductase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase of the organs were measured spectrophotometrically. Our hypothesis was that enhanced environmental pressure during the summer season, induced by multiple factors (biogenic, abiogenic and anthropogenic) led to weakening of the antioxidant protection in mussels at the beginning of autumn. The reaction of the mussel organism to the multiple stress factors was specific for the target organ and the type of the biomarker. Significant differences were present in the activity of the antioxidant system in mussels from the northern and southern coastal locations. The seasonal changes in the pro/antioxidant status of mussels were primarily due to specific seasonal changes in factors concerning the marine environment at the concrete locality. Further research is obviously needed to confirm the present results and provide a more complete data of seasonal and spatial changes in the antioxidant defense system of mussels from the Bulgarian Black Sea coastal area and their implementation in biomonitoring programs.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48998824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the mode of action of Origanum vulgare spp. hirtum methanolic extract and essential oil on Chlamydomonas reinhardtii On土豆泥甲醇提取物和精油对莱茵衣藻的作用模式

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77313

M. D. Todorova, P. Parvanova, T. Todorova, Georgi D. Dronchev, M. Nikolova, S. Berkov, S. Chankova

{"title":"On the mode of action of Origanum vulgare spp. hirtum methanolic extract and essential oil on Chlamydomonas reinhardtii","authors":"M. D. Todorova, P. Parvanova, T. Todorova, Georgi D. Dronchev, M. Nikolova, S. Berkov, S. Chankova","doi":"10.3897/biorisk.17.77313","DOIUrl":"https://doi.org/10.3897/biorisk.17.77313","url":null,"abstract":"Aim: To reveal whether methanolic extract and essential oil from Origanum vulgare subsp. hirtum in doses causing even low levels of mortality in aphids, would have harmful effects on plants-genotoxic, mutagenic and/or DNA damaging. Materials and methods: Aerial parts of Origanum vulgare ssp. hirtum from the ex-situ collection of IBER, BAS during flowering were collected. Extraction and isolation procedures, as well as GC/MS analysis of essential oil and methanolic extract were performed by standard protocols. The components were identified by comparing their relative retention times to the retention times of authentic standards, and with mass spectra with the NIST. Test system: Chlamydomonas reinhardtii strain 137 C+ (WT). Endpoints: “clonal” assay, the test of “visible mutations”, constant field gel electrophoresis. Statistics: GraphPad Prism version 6.04 (San Diego, USA) and One-way Analysis of Variance ANOVA with multiple comparisons using the Tukey method. Results: A good correlation was observed between chemical composition of essential oil and methanolic extract, and their mode of action. Our genotoxic and double strand breaks results demonstrated mild genotoxic and statistically non-significant DNA damaging potential of methanolic extract and concentration-dependent well - expressed genotoxic and DNA damaging potential of essential oil. A good relationship between increased double strand breaks levels and decreased survival might be related to one of the main constituents of essential oil, suspected to be carvacrol. No mutagenic effect for ME and EO was found. Conclusion: Well-expressed toxic/genotoxic capacity of essential oil, as well as its capacity to damage DNA inducing double strand breaks, but the absence of mutagenic potential, could be considered as a good reason to recommend Origanum vulgare subsp. hirtum essential oil as a promising candidate for purposes of “green” technologies.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44458512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant products with acetylcholinesterase inhibitory activity for insect control 具有乙酰胆碱酯酶抑制活性的昆虫防治植物产品

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77052

B. Georgiev, M. Nikolova, I. Aneva, A. Dzhurmanski, B. Sidjimova, S. Berkov

{"title":"Plant products with acetylcholinesterase inhibitory activity for insect control","authors":"B. Georgiev, M. Nikolova, I. Aneva, A. Dzhurmanski, B. Sidjimova, S. Berkov","doi":"10.3897/biorisk.17.77052","DOIUrl":"https://doi.org/10.3897/biorisk.17.77052","url":null,"abstract":"Acetylcholinesterase (AChE) inhibitors are widely used in Alzheimer’s treatment, but they are also crucial for their action on organophosphorus insecticides. The latter exert their toxicity by inhibiting the AChE enzyme in insects, leading to their death. Amaryllidaceae alkaloids have been proven to be potent AChE inhibitors. In the present study methanolic extracts and essential oils being obtained from species of Asteraceae, Lamiaceae, Brassicaceae and Amaryllidaceae were evaluated in vitro for AChE inhibitory activity. Ellman’s colourimetric method, with modifications, was used for AChE activity evaluation. According to the activity level, the tested plant products were divided into three categories. First: plant products with strong activity comparable to that of galanthamine; second: plant products with medium activity, with IC50 value about 1 mg/ml and the last group with low activity, with IC50 value greater than 1 mg/ml. Essential oils of Origanum vulgare subsp. hirtum Ietswaart., Satureja pilosa Vel., Monarda fistulosa L., Thymus longedentatus (Degen & Urum.) Ronniger and the methanolic extract of Leucojum aestivum L. showed the most potent activity and were referred to as the first group. Carvacrol was identified as the main component of the most active essential oils. In L. aestivum extract, galanthamine was found as the main alkaloid. The obtained results indicate that essential oils and alkaloid-rich plant extracts possess the strongest AChE inhibitory activity. This gives us a reason to recommend these plant products to be tested for insecticidal activity in the future.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48400921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Correlation between bacterial abundance, soil properties and heavy metal contamination in the area of non-ferrous metal processing plant, Southern Bulgaria Correlation在保加利亚南部有色金属加工厂区域细菌丰度、土壤性质和重金属污染之间的关系

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77458

Radina Nikolova, Michaella Petkova, N. Dinev, A. Kenarova, S. Boteva, D. Berov, G. Radeva

引用次数: 3

Application of residual sludges from wastewater treatment technologies for construction of biofertiliser 污水处理剩余污泥在生物肥料建设中的应用

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77463

M. Belouhova, Dobromira Yaneva, Y. Topalova

{"title":"Application of residual sludges from wastewater treatment technologies for construction of biofertiliser","authors":"M. Belouhova, Dobromira Yaneva, Y. Topalova","doi":"10.3897/biorisk.17.77463","DOIUrl":"https://doi.org/10.3897/biorisk.17.77463","url":null,"abstract":"To stimulate plant development in phytoremediation or in the cultivation of non-food crops in potentially contaminated soils, a biotechnologically created product could be applied. The aim of this study was to explore the possibility of creation of biofertiliser, based on activated sludge combined with bacterial strain with detoxifying and plant growth promoting properties. The presented study is focused on the effect of phenol in the following concentrations: 5 mg/l, 100 mg/l, 250 mg/l, 500 mg/l and 1000 mg/l on the metabolic activity of Brevibacillus laterosporusBT271. The gradual increased concentration of phenol was used to study the metabolic activity of mineralised activated sludge and B. laterosporusBT271. The CTC/DAPI staining showed high activity of the bacteria even at the highest concentration. The greatest amount of biomass was accumulated at 5 mg/l phenol (4.44 × 107 cells/ml). At this toxicant concentration, a total dehydrogenase activity of 5.72 × 10-4 μg H+/ml*min was found. Studies of the metabolic activity of microorganisms in experiments involving a combination of mineralised activated sludge, B. laterosporusBT271 and phenol at three concentrations (5 mg/l, 250 mg/l and 1000 mg/l) showed the highest value for dehydrogenase activity in the variant with average phenolic concentration (up to 6.39 × 10-6 μg H+/ml*min. The results proved the detoxification potential of B. laterosporusBT271 when different concentrations of phenol were present. The combination of a mineralised activated sludge and selected highly active biodegrading B. laterosporusBT271 showed valuable properties of detoxification and metabolic activity and keep these potentials up to 1000mg/l phenol.","PeriodicalId":38674,"journal":{"name":"BioRisk","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41747063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of some environmental factors on the distribution of zooplankton complexes in Mandra Reservoir, in Southeastern Bulgaria 保加利亚东南部Mandra水库一些环境因素对浮游动物群落分布的影响

BioRisk Pub Date : 2022-04-21 DOI: 10.3897/biorisk.17.77368

Eleonora Fikovska, D. Kozuharov, M. Stanachkova

引用次数: 1

BioRisk最新文献